1.Susceptibility of experimental animals to reinfection with Clonorchis sinensis.
Woon Mok SOHN ; Hongman ZHANG ; Min Ho CHOI ; Sung Tae HONG
The Korean Journal of Parasitology 2006;44(2):163-166
The present study observed the resistance to reinfection with Clonorchis sinensis in various experimental animals including mice, guinea pigs, rabbits, and dogs, as well as rats and hamsters. The resistance rates to reinfection in rats, mice, hamsters, guinea pigs, rabbits, and dogs were 79.7%, 58.0%, -12.6%, 54.8%, 62.6%, and 6.0%, respectively. Worms recovered from reinfected rats and mice were immature, and significantly smaller than those from the primarily infected (P < 0.01), whereas those from other animals were fully matured to adults. These findings indicate that the protective response against reinfection with C. sinensis is prominent in rats and mice, and that they may be a good animal model to investigate the mechanism of resistance to reinfection with C. sinensis.
Rats
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Rabbits
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Praziquantel/therapeutic use
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Mice
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Guinea Pigs
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Dogs
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Cricetinae
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Clonorchis sinensis/immunology/*physiology
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Clonorchiasis/drug therapy/immunology
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Anthelmintics/therapeutic use
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Animals, Laboratory/immunology/*parasitology
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Animals
2.Evaluation of IgG4 Subclass Antibody Detection by Peptide-Based ELISA for the Diagnosis of Human Paragonimiasis Heterotrema.
Pewpan M INTAPAN ; Oranuch SANPOOL ; Penchom JANWAN ; Porntip LAUMMAUNWAI ; Nimit MORAKOTE ; Yoon KONG ; Wanchai MALEEWONG
The Korean Journal of Parasitology 2013;51(6):763-766
A synthetic peptide was prepared based on the antigenic region of Paragonimus westermani pre-procathepsin L, and its applicability for immunodiagnosis for human paragonimiasis (due to Paragonimus heterotremus) was tested using an ELISA to detect IgG4 antibodies in the sera of patients. Sera from other helminthiases, tuberculosis, and healthy volunteers were used as the references. This peptide-based assay system gave sensitivity, specificity, accuracy, and positive and negative predictive values of 100%, 94.6%, 96.2%, 100%, and 88.9%, respectively. Cross reactivity was frequently seen against the sera of fascioliasis (75%) and hookworm infections (50%). Since differential diagnosis between paragonimiasis and fascioliasis can be easily done by clinical presentation and fascioliasis serology, this cross reaction is not a serious problem. Sera from patients with other parasitoses (0-25%) rarely responded to this synthetic antigen. This synthetic peptide antigen seems to be useful for development of a standardized diagnostic system for paragonimiasis.
Adult
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Animals
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Antibodies, Helminth/*blood
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Antigens, Helminth/*diagnostic use
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Clinical Laboratory Techniques/*methods
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Cross Reactions
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Enzyme-Linked Immunosorbent Assay/methods
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Humans
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Immunoglobulin G/*blood
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Paragonimiasis/*diagnosis
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Paragonimus/*immunology
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Parasitology/*methods
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Predictive Value of Tests
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Sensitivity and Specificity