2.A comparison of metabolomic changes in type-1 diabetic C57BL/6N mice originating from different sources.
Seunghyun LEE ; Jae Hwan KWAK ; Sou Hyun KIM ; Jieun YUN ; Joon Yong CHO ; Kilsoo KIM ; Daeyeon HWANG ; Young Suk JUNG
Laboratory Animal Research 2018;34(4):232-238
Animal models have been used to elucidate the pathophysiology of varying diseases and to provide insight into potential targets for therapeutic intervention. Although alternatives to animal testing have been proposed to help overcome potential drawbacks related to animal experiments and avoid ethical issues, their use remains vital for the testing of new drug candidates and to identify the most effective strategies for therapeutic intervention. Particularly, the study of metabolic diseases requires the use of animal models to monitor whole-body physiology. In line with this, the National Institute of Food and Drug Safety Evaluation (NIFDS) in Korea has established their own animal strains to help evaluate both efficacy and safety during new drug development. The objective of this study was to characterize the response of C57BL/6NKorl mice from the NIFDS compared with that of other mice originating from the USA and Japan in a chemical-induced diabetic condition. Multiple low-dose treatments with streptozotocin were used to generate a type-1 diabetic animal model which is closely linked to the known clinical pathology of this disease. There were no significantly different responses observed between the varying streptozotocin-induced type-1 diabetic models tested in this study. When comparing control and diabetic mice, increases in liver weight and disturbances in serum amino acids levels of diabetic mice were most remarkable. Although the relationship between type-1 diabetes and BCAA has not been elucidated in this study, the results, which reveal a characteristic increase in diabetic mice of all origins are considered worthy of further study.
Amino Acids
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Amino Acids, Branched-Chain
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Animal Experimentation
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Animal Testing Alternatives
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Animals
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Ethics
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Japan
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Korea
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Liver
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Metabolic Diseases
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Metabolomics*
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Mice*
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Models, Animal
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Pathology, Clinical
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Physiology
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Streptozocin
3.Role of Echocardiography in Small Animal Research.
Journal of the Korean Society of Echocardiography 1999;7(1):5-11
No abstract available.
Animal Experimentation*
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Animals*
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Echocardiography*
4.Continue to study on influences of Morinda citrifolia L. Rubiaceae fruits on experimental animals immuno-suppressed by radiation
Pharmaceutical Journal 2005;347(3):16-19
Study on the extract of Morinda citrifolia (MC) fruit with the ratio of 5:1 (1g extract was prepared from 5g of dried MC fruit) in order to determine the effects of extract on radiation-induced immunosuppressive mice. The results showed that: oral administration of this extract with daily dose of 6 g/kg in 9 consecutive days (3 days before and 6 days during receiving gamma ray) has increased the relative weight of spleen and thymus, total leukocyte count, number of lymphocytes, natural killers and monocytes, rate of immune rosette forming cells, hemolytic halo, and the skin test with OA antigen, compared with the mice group that has been only received gamma ray without drug
Morinda
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Animal Experimentation
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Radiation
5.Experimental study on hemoregulation effects of cracetin
Journal of Practical Medicine 2005;530(11):39-42
Method of dissemination intravascular coagulation (DIC) was used on experimental rabbits by intravenous thrombin solution 15 IU. Then rabbits were divided into 3 groups: the control group (8 rabbits) received NaCI 0.9% solution 2 ml/kg W.b/24h; the study group (10 rabbits) received mixed pollution cracetin 10.5 mg/kg/24h; the compare drug group (10 rabbits) received Tanakan solution 4.8 mg/kg w.b. All 3 rabbits groups were orally received in one continuous week. On experimental model of DIC at the dose of 10.5 mg/kg W.b/24h orally within 7 days, cracetin elongated the values of the Howell time, Quick time, thrombin time, partial thrombin time. The drug cracetin increased amount of fibrinogen, restored the number of thrombosis that were consumed more in the progress of forming blood coagulation before.
Blood
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Animal Experimentation
6.Sub-chronic toxicity of 16-piperazinoethanol-10 alpha-triluoromethyl anhydroihydroartemisinin (BB134) in experimental animal
Journal of Malaria and parasite diseases Control 2003;0(6):15-22
The BB134's sub-chronic toxicity in rabbits was investigated at NIMPE's laboratory. BB134 was orally administered at the dose of 9mg/kg of body weight per day for 28 consecutive days. The influence of BB134 on rabbit's laboratory indices and cardiovascular system were observed during and after the BB134 administration. The BB134 had not change the normal indices of development as well as the biochemical and some hematological indices of the experimental rabbits. During the study time the rabbits acted and ate normally. The body weight was increased significantly. SGOT, SGPT, bilirubin and creatinine as well as leukocytes, leukocyte formula and hemoglobin had no change. However erythrocytes decreased significantly by day 14. BB134 was also found not to affect significantly on rabbits' cardiovascular system (rabbits' heart rhythms and cardiovascular waves such as P, QP, QRS, T and QT of the control and treated groups were not changed significantly
Malaria
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Animal Experimentation
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Artemisinins
7.Evaluation of Dose Distribution Using a Radiophotoluminescence Glass Dosimeter in Biobeam8000 Gamma Irradiation Device.
Sang Hun SHIN ; Sunghyun LEE ; Kihong SON ; Hyun Ho LEE ; Kum Bae KIM ; Haijo JUNG ; Young Hoon JI
Korean Journal of Medical Physics 2011;22(4):198-205
Gamma irradiator is widely used for cell, animal experiment, irradiation for blood, dose measurement, and education. Biobeam8000 gamma irradiator (STS Steuerungstechnik &. Strahlenschutz GmbH, Braunschweig, Germany, Cs137, 81.4 TBq) that KIRAMS (Korea Institute of Radiological and Medical Science) has is a irradiation device that enables to be used in large-capacity of 7.5 L and extensive area. Cs-137 source moves range of 24 cm back-and-forth in a regular cycle in beaker for uniform irradiation and a beaker that puts a specimen like existing radiation irradiator such as Gammacell3000 rotates 360degrees during irradiation. Precise dose information according to the location of radiation source would be needed because of the movement of radiation source, whereas radiation could be uniformly irradiated in comparison with existing gamma irradiator. In this study, dose distribution of the inside beaker located in Biomeam8000 gamma irradiator was measured using glass dosimeter, and dose evaluation and distribution regarding dose linearity and dose reproducibility were implemented based on measurement results. This aims to show guideline for efficient use of irradiator based on measurement result when doing experiment or radiation exposure.
Animal Experimentation
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Germany
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Glass
8.The extrapolation method in dosage is equivalent in effectiveness between man and experimental animals
Pharmaceutical Journal 1999;282(10):7-9
Introduction on the factors influence dosage that is equivalent in effectiveness between animals. They comprised drug metabolism, minimum concentration have with effect in serum, the effective time, cellular respiration, organic weight rate comparison with body weight, heat production, cytochrom C, volume of kidney and body surface.
Dosage Forms
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Animal Experimentation
9.Study on the roborant effect of drug by swimming test in the experimental animal
Pharmaceutical Journal 1999;274(2):7-9
A study on the roborant effect of drug by swimming test in the experimental rat or mice was carried out. The swimming time calculated from standing swimming until rat was exhausted that can not swim. There were 2 method of calculation, including average swimming time and second swimming time comparing with the first swimming time.
Pharmaceutical Preparations
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Animal Experimentation
10.Evaluate the influences of pentafluoropro pyloxydihydroartemisinin and mefloquine on some nervous functions of white rats
Pharmaceutical Journal 2005;347(3):12-16
The study was carried out on 100 healthy rats, weight of 12020g which were divided into 10 groups. Raw materials: BB103 pure powder and mefloquine. Results: In rats received BB103 with doses of 50 mg/kg and mefloquine 50 mg/kg in 5 days, there were no significant changes on process of conditioned reflex, speed of reflex establishment was stable, response time and extinguishing time of reflex were similar to those of the control group (p>0.05). In rats received BB103 with doses of 100 mg/kg and mefloquine 100 mg/kg in 5 days, there were significant changes on speed of reflex establishment and response time (p<0.01-0.05). There were differences on speed of reflex establishment, speed of stable reflex in rats received BB103 and mefloquine (p<0.01-0.05)
Artemisinins
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Rats
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Animal Experimentation