1.Infantile scurvy masquerading as bone tumour.
Rehan Ul HAQ ; Ish Kumar DHAMMI ; Anil K JAIN ; Puneet MISHRA ; K KALIVANAN
Annals of the Academy of Medicine, Singapore 2013;42(7):363-365
Ascorbic Acid
;
administration & dosage
;
Child
;
Child Nutrition Disorders
;
complications
;
diet therapy
;
Diagnosis, Differential
;
Femoral Neoplasms
;
diagnosis
;
Femur
;
diagnostic imaging
;
Humans
;
Male
;
Protein-Energy Malnutrition
;
complications
;
diet therapy
;
Radiography
;
Scurvy
;
diagnosis
;
diet therapy
;
etiology
;
physiopathology
;
Treatment Outcome
2.Expression and lytic efficacy assessment of the Staphylococcus aureus phage SA4 lysin gene.
Anil Kumar MISHRA ; Mayank RAWAT ; Konasagara Nagaleekar VISWAS ; ABHISHEK ; Sujeet KUMAR ; Manjunatha REDDY
Journal of Veterinary Science 2013;14(1):37-43
Treatment of bovine mastitis caused by Staphylococcus (S.) aureus is becoming very difficult due to the emergence of multidrug-resistant strains. Hence, the search for novel therapeutic alternatives has become of great importance. Consequently, bacteriophages and their endolysins have been identified as potential therapeutic alternatives to antibiotic therapy against S. aureus. In the present study, the gene encoding lysin (LysSA4) in S. aureus phage SA4 was cloned and the nucleotide sequence was determined. Sequence analysis of the recombinant clone revealed a single 802-bp open reading frame encoding a partial protein with a calculated mass of 30 kDa. Results of this analysis also indicated that the LysSA4 sequence shared a high homology with endolysin of the GH15 phage and other reported phages. The LysSA4 gene of the SA4 phage was subsequently expressed in Escherichia coli. Recombinant LysSA4 induced the lysis of host bacteria in a spot inoculation test, indicating that the protein was expressed and functionally active. Furthermore, recombinant lysin was found to have lytic activity, albeit a low level, against mastitogenic Staphylococcus isolates of bovine origin. Data from the current study can be used to develop therapeutic tools for treating diseases caused by drug-resistant S. aureus strains.
Animals
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Base Sequence
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Cloning, Molecular
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Gene Expression Regulation, Viral/physiology
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Mucoproteins/genetics/*metabolism
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Phylogeny
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Polymerase Chain Reaction/methods
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Recombinant Proteins
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Staphylococcus Phages/genetics/*metabolism/physiology
;
Staphylococcus aureus/*virology
3.Morin ameliorates myocardial injury in diabetic rats via modulation of inflammatory pathways
Vipin Kumar VERMA ; Salma MALIK ; Ekta MUTNEJA ; Anil Kumar SAHU ; Vaishali PRAJAPATI ; Prashant MISHRA ; Jagriti BHATIA ; Dharamveer Singh ARYA
Laboratory Animal Research 2024;40(1):51-63
Background:
High blood glucose levels in diabetes lead to vascular inflammation which accelerates atherosclerosis. Herein, Morin was orally administered in male Wistar rats, at the dose of 40 mg/kg for 28 days, and on the 27th and 28th day, ISO was administered to designate groups at the dose of 85 mg/kg s.c., to induce myocardial infarction.
Results:
Free radical generation, including ROS, in diabetes following ISO administration, leads to the activation of both intrinsic and extrinsic pathways of apoptosis. Morin significantly (p ≤ 0.05) reduced oxidative stress (GSH, MDA, SOD), cardiac injury markers (CK-MB, LDH), inflammation (TNF, IL-6), and apoptosis (Bax, BCl 2 , Caspase-3). In addition, it also reduced insulin and blood glucose levels. Akt/eNOS, Nrf2/HO-1, MAPK signaling pathways, and Insulin signal transduction pathways were positively modulated by morin pre-treatment.
Conclusions
Morin attenuated oxidative stress and inflammation and also modified the activity of various molecular pathways to mitigate cardiomyocyte damage during ISO-induced MI in diabetic rats.