1.Angiopoietin-2 and tumor angiogenesis
Journal of International Oncology 2012;39(5):352-355
Tumor angiogenesis is an important way of rapid proliferation and metastasis for malignancies,and a variety of cellular and molecular factors involved in the process.Angiopoietin-2(Ang-2) is one of the important vascular endothelial growth factor,which influences tumor angiogenesis mainly through releasing vascular structures and damaging the vessel stability.Recent researches reveal that Ang-2 modulates tumor angiogenesis interacting with other vascular growth factors.With the new research progress,Ang-2 will not only be an important target for antiangiogenic therapy,but also further improve clinical efficacy of antitumor therapy combined with other signal pathways as common targets.
2.Therapeutic Observation of Acupuncture-moxibustion plusMEI Te-lan’s Manipulations for Lumbar Intervertebral Disc Herniation (Blood Stagnation Pattern)
Anhong LI ; Shuangchun AI ; Hong ZHANG ; Longdi WU ; Jing CHEN
Shanghai Journal of Acupuncture and Moxibustion 2016;(1):58-62
Objective To observe the clinical efficacy of acupuncture-moxibustion plusMEI Te-lan’s manipulations in treating lumbar intervertebral disc herniation (LIDH) due to blood stagnation.Method Totally 248 LIDH patients due to blood stagnation were randomized into a treatment group of 123 cases and a control group of 125 cases. The treatment group was intervened by acupuncture-moxibustion plusMEI Te-lan’s manipulations, while the control group was by acupuncture-moxibustion plus tuina at lower back. The short-form McGill Pain Questionnaire (SF-MPQ) and Oswestry disability index (ODI) were observed before and after intervention, and the clinical efficacies were compared between the two groups.Result The total effective rate and recovery-markedly effective rate were respectively 80.5% and 52.0% in the treatment group, versus 72.8% and 20.0% in the control group. There was a significant difference in comparing the recovery-markedly effective rate between the two groups (P<0.05). In the treatment group, the Pain Rating Index (PRI) score, Visual Analogue Scale (VAS) score, Present Pain Intensity (PPI) score, and ODI score were significantly changed after 3-day, 6-day, and 20-day treatment compared to the scores before treatment (P<0.05). After 20-day treatment, the PRI, VAS, PPI, and ODI scores of the treatment group were significantly different from that of the control group (P<0.05).Conclusion Acupuncture-moxibustion plusMEI Te-lan’s manipulations is an effective method in treating LIDH due to blood stagnation.
3.Overexpression of Spinacia oleracea betaine aldehyde dehydrogenase (SoBADH) gene confers the salt and cold tolerant in Gossypium hirsutum L.
Xiaoli LUO ; Juanli XIAO ; Zhian WANG ; Anhong ZHANG ; Yingchuan TIAN ; Jiahe WU
Chinese Journal of Biotechnology 2008;24(8):1464-1469
The open reading frame of Spinacia oleracea Betaine Aldehyde Dehydrogenase (SoBADH) was retrieved from Spinacia oleracea and inserted into the Agrobacterium tumefaciens binary vector pBin438, which was driven by CaMV35S promoter, and produced the new binary vector pBSB. A. tumefaciens LBA4404 carrying this plasmid was used in genetic transformation of plants. Forty-five primary transgenic plants were detected by PCR and verified by the Southern blotting from 65 regenerated plants, of which 27 transgenic plants had only one copy of T-DNA. The Northern blotting and Western blotting analysis indicated that the SoBADH gene had been transcribed mRNA and expression protein in the transgenic cotton lines. The testing of SoBADH activity of transgenic plant leaves showed that the enzyme activity was much higher than that of the non-transgenic cotton. The growth of transgenic plants was well under the salinity and freezing stress, whereas the non-transgenic plant grew poorly and even died. Challenging with salinity, the height and fresh weight of transgenic plants was higher compared with those of non-transgenic plants. Under the freezing stress, the relative conductivity of leaf electrolyte leakage of the transgenic cotton lines was lower than that of non-transgenic plants. These results demonstrated that the SoBADH gene could over express in the exogenous plants, and could be used in genetic engineering for cotton stress resistance.
Adaptation, Physiological
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Betaine-Aldehyde Dehydrogenase
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biosynthesis
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genetics
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Cold Temperature
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Gossypium
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enzymology
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genetics
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Plants, Genetically Modified
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enzymology
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genetics
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Salinity
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Spinacia oleracea
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enzymology
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genetics
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Stress, Physiological
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genetics
4.Impact of the interaction between metabolic syndrome and smoking on the risk of cardiovascular events
Anhong ZHENG ; Nianchun PENG ; Miao ZHANG ; Qiao ZHANG ; Lixin SHI ; Ying HU ; Rui WANG ; Juan HE
Chinese Journal of Endocrinology and Metabolism 2023;39(7):581-587
Objective:To investigate the effect of the interaction between metabolic syndrome and smoking on the risk of subsequent cardiovascular events.Methods:Urban residents aged 40 and above in the Yunyan District of Guiyang City were selected from " Risk Evaluation of cAncers in Chinese diabeTic Individuals: A lONgitudinal(REACTION) Study". The baseline survey started in 2011 and general information including gender, age, medical history, lifestyle habits, and smoking status were collected. Additionally, biochemical indicators related to metabolic syndrome(MS) were measured. The study participants were then followed up, and the first cardiovascular events occurring after the initial survey were recorded. The average follow-up period was 10.07±1.49 years. The interaction between metabolic syndrome and smoking on subsequent cardiovascular events was analyzed using Cox proportional hazards models.Results:The study included a total of 7 275 individuals, among whom 639 experienced cardiovascular events. After adjusting for multiple variables, compared to non-smokers without metabolic syndrome(MS), smokers with MS showed a higher risk of cardiovascular events, with a hazard ratio( HR) of 6.54(95% CI 4.88, 8.78). This risk was higher than that of individuals with MS who never smoked [ HR 1.39(95% CI 1.11, 1.75)] and non-MS smokers [ HR 2.48(95% CI 1.77, 3.49)]. There was an additive interaction between MS and smoking on the occurrence of cardiovascular events, with a relative excess risk due to interaction(RERI) of 3.30(95% CI 1.89, 4.70), an attributable proportion(AP) of 0.55(95% CI 0.43, 0.59), and a synergy index(S) of 3.07(95% CI 1.94, 4.84). Furthermore, when stratifying the duration of smoking cessation, long-term quitters(≥8 years) showed a lower risk of cardiovascular events compared to current smokers, regardless of whether they had MS. The hazard ratios were 0.45(95% CI 0.26, 0.78) for individuals with MS and 0.42(95% CI 0.19, 0.95) for individuals without MS. Conclusions:There is an additive interaction between smoking and MS on the risk of cardiovascular events. The coexistence of both factors significantly increases the risk of cardiovascular events.
5.Research advances of portal vein thrombosis in perioperative period of liver transplantation
Junkai FENG ; Anhong ZHANG ; Zhening YAN ; Yanbo MA
Chinese Journal of Organ Transplantation 2023;44(4):243-251
Correlated with such hepatic-systemic factors as cirrhosis, inflammation and immunity, portal vein thrombosis (PVT) is common in perioperative period of liver transplantation (LT) recipients.It affects negatively surgical procedures and outcomes due to its insidious onset and atypical clinical symptoms.With continuous improvements of LT techniques and refining of medical imaging, researchers have gained further insights into the pathophysiological processes, screening, diagnoses, evaluations, classifications and perioperative managements of PVT.This review focused upon perioperative managements of LT recipients with PVT to enhance the clinical problem-solving capability and long-term patient survival.
6.Clinical study of novel coronaviral infection in recipients of liver transplantation from deceased donation
Rui ZHANG ; Hezhao ZHANG ; Zhiyong SHI ; Anhong ZHANG ; Jun XU
Chinese Journal of Organ Transplantation 2023;44(11):682-688
Objective:To explore the effects of novel coronaviral infection on the outcomes of liver transplantation recipients.Methods:The relevant clinical data were retrospectively reviewed for 5 liver transplantation recipients infected with novel coronavirus during perioperative period at First Hospital of Shanxi Medical University.At the same time, "liver transplantation" and "novel coronavirus" and "2019 novel coronavirus" or "SARS-CoV-2" were employed as Chinese and English keywords respectively for searching the relevant literature items of CNKI, Wanfang and PubMed from the inception of database until February 2023.The impact of perioperative novel coronaviral infection was examined on the outcomes of liver transplantation recipients.Results:From November 2022 to January 2023, liver transplantation was performed in 5 recipients of donated liver after death.Two cases tested positive for real-time polymerase chain reaction(RT-PCR)at Day 14/20 pre-operation and converted into negative post-operation.Three cases were infected with SARS-CoV-2 at Day 1/10/16 post-operation.And one of them became feverish again at Day 11 post-infection.With a peak temperature of 39.5 degrees, it rapidly progressed into multiple organ failures of heart, lung and kidney.After active interventions, organ function gradually recovered.Through literature searches, 9 eligible English literature items were retrieved.However, there was no Chinese entry.Among 15 liver transplantation recipients with SARS-CoV-2 infection, one died from septic shock, another from graft failure and the remainders survived.Conclusions:liver transplantation recipients infected with SARS-CoV-2 and negative RT-PCR pre-operation are asymptomatic.Without signs of pneumonia on chest CT, transplantation may be performed.For post-liver transplantation recipients with early SARS-CoV-2 infection, close monitoring of important organ functions such as heart, lung, liver and kidney, timely antiviral regimen and tapered use of immunosuppression can achieve a decent prognosis.
7.Application and clinical evaluation of ultrasound-guided biliary drainage tube replacement technology
Anhong ZHANG ; Ruixin ZHANG ; Jie MA ; Bo QIU ; Xin YI ; Zhihua LU ; Lijie ZHENG ; Hanguang DONG ; Tian HAN ; Li ZHANG ; Yuanhui JIANG ; Jun XU
Journal of Clinical Hepatology 2022;38(11):2542-2545
Objective To summarize the preliminary application results of ultrasound-guided biliary drainage tube replacement, present the corresponding technical points, and discuss the operation strategy and clinical application value. Methods The clinical data of 60 patients who underwent ultrasound-guided biliary drainage tube replacement in Qilu Hospital of Shandong University between August 2014 and August 2020 were retrospectively analyzed. The operation procedure, clinical applications, and postoperative complications were summarized and analyzed. Results Fifty-eight of the 60 patients (96.67%) were successfully replaced with drainage tubes along the original sinus. Among them, dilated sinus tracts of 47 patients were placed with coarse-grade drainage tubes, and dilated sinus tracts of the remaining 11 patients were placed with the original type of drainage tubes, with the mean operation time of 15.8(12.0-19.0) min under local anesthesia. In total, bile was drained from 28 patients receiving PTCD drainage, 23 patients receiving gallbladder drainage, and 9 patients receiving T-tube drainage. The post-operation evaluation revealed that the drainage situation has improved, with a 100% effective rate. No obvious abnormality was found in the postoperative follow-up visit. Conclusion The replacement of drainage tube under ultrasound guidance is simple, safe and feasible, and it provides further promotion in clinical practice with sufficient data support.