Background: Plasma lipids are affected not only by dietary intake and life style but also by genetic factor. Objective: To discover the relationship between plasma lipids and the presence of LPL S447X and PAF-AH V279F in the bodies of rural Vietnamese population. Subject and Method: One hundred and ninety three subjects were randomly selected from rural areas in Ho Chi Minh city. The presence of LPL S447X and PAF-AHV279F polymorphism was determined using an Invader assay, and the plasma lipid levels were measured by an enzymatic method. A profile regarding the dietary intake was also made for each subject. Result and Conclusion: The frequency of LPL 447SX/XX (12.2%) polymorphism, PAF-AH Val279Phe mutation allele frequency (5.4%) in the Vietnamese was closely similar to that of other Asian populations. LPL447X polymorphism was found to be significantly associated with a higher HDL-C level in comparison to LPL 447S. LPL S447X affects plasma HDL-C and TG concentration in Vietnamese rural population.
Genetic factors ; plasma lipids ; rural area

OBJECTIVES: To investigate the relationship between dyslipidemia and obesity status among Viet-namese adolescents. METHODS: In this case-control study, 282 adolescents (6–11 years), including 88 obese cases and 194 normal-weight controls, were recruited from a population-based cross-sectional study from two provinces in Vietnam. The anthropometric, blood lipid, and other laboratory test results of the study subjects were analyzed. RESULTS: Obese children tended to have more visceral fat (Pearson's r = 0.795, p < 0.0001) than subcutaneous fat (Pearson's r = 0.754, p < 0.0001), and this difference was associated with an increase in blood triglyceride level (Pearson's r = 0.232, p < 0.05) and a strikingly high rate of hypertriglyceridemia (38.6%). We also found that birth weight and parental body mass index were related to the status of obesity among the study subjects. However, only birth weight was significantly higher in the obese group than in the normal weight group. These findings indicate the effect of prenatal nutrition on childhood obesity. Furthermore, high-birth weight children had a surprisingly high rate of obesity. CONCLUSION: Together, our data suggest that obesity increased the risk for hypertriglyceridemia, which was, at least partially, due to prenatal nutrition.
Adolescent ; Birth Weight ; Body Mass Index ; Case-Control Studies* ; Child ; Cross-Sectional Studies ; Dyslipidemias ; Humans ; Hypertriglyceridemia* ; Intra-Abdominal Fat ; Obesity ; Parents ; Pediatric Obesity* ; Subcutaneous Fat ; Triglycerides ; Vietnam*

Activation of Toll-like receptor-4 (TLR-4) in articular chondrocytes increases the catabolic compartment and leads to matrix degradation during the development of osteoarthritis. In this study, we determined the proteomic and genomic alterations in human chondrocytes during lipopolysaccharide (LPS)-induced inflammation to elucidate the underlying mechanisms and consequences of TLR-4 activation. Human chondrocytes were cultured with LPS for 12, 24, and 36 h to induce TLR-4 activation. The TLR-4-induced inflammatory response was confirmed by real-time PCR analysis of increased interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF-α) expression levels. In TLR-4-activated chondrocytes, proteomic changes were determined by two-dimensional electrophoresis and matrix-assisted laser desorption/ionization-mass spectroscopy analysis, and genomic changes were determined by microarray and gene ontology analyses. Proteomics analysis identified 26 proteins with significantly altered expression levels; these proteins were related to the cytoskeleton and oxidative stress responses. Gene ontology analysis indicated that LPS treatment altered specific functional pathways including ‘chemotaxis’, ‘hematopoietic organ development’, ‘positive regulation of cell proliferation’, and ‘regulation of cytokine biosynthetic process’. Nine of the 26 identified proteins displayed the same increased expression patterns in both proteomics and genomics analyses. Western blot analysis confirmed the LPS-induced increases in expression levels of lamin A/C and annexins 4/5/6. In conclusion, this study identified the time-dependent genomic, proteomic, and functional pathway alterations that occur in chondrocytes during LPS-induced TLR-4 activation. These results provide valuable new insights into the underlying mechanisms that control the development and progression of osteoarthritis.
Annexins* ; Blotting, Western ; Chondrocytes* ; Cytoskeleton ; Electrophoresis ; Gene Ontology ; Genomics ; Humans* ; Inflammation ; Interleukin-1beta ; Interleukin-6 ; Osteoarthritis ; Oxidative Stress ; Proteomics ; Real-Time Polymerase Chain Reaction ; Spectrum Analysis ; Tumor Necrosis Factor-alpha

Background: Hypertension is a common disease in adults, also the leading cause of cardiovascular disease and premature death worldwide. The prevalence of hypertension has increased, especially in low- and middle-income countries. Therefore, hypertension screening is important to provide the basis and evidence for the development of timely intervention policies and programs. The objective of the “May Measurement Month” 2022 program is to investigate the prevalence of hypertension, drug treatment and blood pressure control in people in Thua Thien Hue province and to assess the risk factors surrounding hypertension. Subjects and Methods: This cross–sectional study collected data from volunteer adults (≥ 18 years old) in Thua Thien Hue province from 7/2022 to 8/2022. Sitting blood pressure had been measured in triplicate according to standardized specified methods of the International Society of Hypertension. Results: Average age 54.07 ± 18.17, there were 627 people (23.4%) with hypertension at the time of screening, 245 of which (39.1%) had never been diagnosed with hypertension before screening at the time. 724 people were on treatment with 1 or more types of drug, however, there were 337 people (46.5%) having their blood pressure not controlled. The statistics showed that 84.9% of volunteers were using at least 1 drug to treat hypertension. Related factors of hypertension are age, sex, overweight/obesity status, smoking, medical history of diabetes and medical history of COVID-19. The rate of subjects who had history of COVID-19 was 36.4%, including 31.6% of hypertension patients. Conclusion: The hypertension percentage of Thua Thien Hue population is still high and the rate of blood pressure control is still limited. There is a relationship between hypertension proportion and history of COVID-19. It is necessary to publish timely intervention measures to reduce the rate of hypertension and its risks to people.

Backgroud: The aim of this study is to update on antibiotic resistance of common pathogenical bacteria isolated in Hue University of Medicine and Pharmacy Hospital (Hue UMP Hospital). Methodology: Use of the agar disk diffusion method to test the susceptibility to antimicrobial agents of 3709 bacterial strains from infected patients hospitalized in Hue UMP Hospital in 2020 - 2022. Results: Among 3709 strains of pathogenical bacteria isolated, S.aureus was found with the rate of 29.9%, followed by E. coli (24.5%), Pseudomonas aeruginasa (17.8%), Enterococcus spp. (11.8%), Klebsiella spp (9.7%) and Acinetobacter spp (4.1%). The proportion of bacterial isolates has changed, but Staphylococcus aureus is still highest rate. S.aureus is resistant to many antibiotics, but MRSA strains have decreased significantly, from 73.3% in 2020 to 62.5% in 2022. Pseudomonas aeruginosa was resistant to some of the group A recommended antibiotics such as ceftazidime, piperacillin-tazobactam with the rate of 56.6% and 48.7%. The percentage of E. coli with ESBL strains (+) was at 28.2% - 30.3%. Enterococus spp strains are still sensitive to vancomycin (83.1% - 91.9%). The rate of Klebsiella ESBL (+) is only 6.9% to 8.2%. The strains of Acinetobacter spp were highly resistant to Piperacillin (100%) and Ceftriaxone (96.5%) but they are still sensitive to imipenems 70 - 71%, highly sensitive to Doxycillin (95.2%) and Cefotaxime (88.4%). Conclusion: Many bacterial strains are resistant to many commonly antibiotics. Providing timely, regular, and effective management of antibiotic resistance patterns for common pathogenic bacteria in hospitals, will help reduce the risk of bacterial resistance.