Acetonitril was used to extract ketoprofen from plasma. The revese phase HPLC was carried out with UV detector at 253nm column Nucleosil ODS 5m (4,6*250 mm); mobile phase: MeCN: AcOH: Triethylamin: H2O (50: 0.5: 0.3: 48.7 v/v); flow rate 0.85 ml/min. Experimental results proved that the method was stable, accurate and precise
Plasma ; Ketoprofen ; Chromatography, High Pressure Liquid

120 healthy subjects were selected and divided into two groups to study the cutaneous conductivity at 12 couples of Yuan point, group 1 including 60 subjects aged between 21 to 25 (30 males, 30 females), group 2 including 60 subjects aged between 50 to 67 (30 males, 30 females). The results: there was no difference in cutaneous conductivity at 12 couple of Yuan point of the lest and right body in both groups of ages and sexes. In female, cutaneous conductivity at 12 couple of Yuan point were diferent depending on menstrual cycle, considerably lower in the latter haft of the cycle compared to the first half of the cycle. In the same age group, cutaneous conductivity at 12 couple of Yuan point in male was lower than that in female. In the same gender group, cutaneous conductivity at 12 couple of Yuan point of the group 1 was lower that of the group 2
Administration, Cutaneous ; Adult ; Medicine, Traditional

At the National Institute of Pediatrics, the cause of acute respiratory inflammation was studied. Most of pathogenic bacteria isolated from patients with pneumonia and bronchitis were S.pneumoniae (48%) and H.influenzae (44%). The methods using antibiotics dilution on agar to determine the minimum concentration of bacteria and PCR to determine the penicillin resistant gene sequences were used. Results showed that 58% of strains of S.pneumoniae were resistant to penicillin with a sensitivity 19%; 74% resistant to cefotaxin, sensitivity 16%. Other beta-lactamase antibiotics except ampicillin (resistance 35%, sensitivity 30%)
Anti-Bacterial Agents ; bacteria ; Respiratory Tract Diseases

Garlic (Allium sativum L.) belongs to the family Liliaceae. It has been used worldwide for long time as spice and folk medicine. Sulfur-containing compounds from Allium sativum L. are especially noticed because these compounds in Allium sativum L. is significantly higher than those in other plants, and due to its pharmacological activities in sulfur-containing medications such as penicillin and sulfonamide antibiotics. From the liquid obtained by garlic cloves press, four sulfur-containing compounds were isolated. Their structures have been elucidated using MS and NMR spectroscopic methods to be ajoene (1), 2-vinyl-4H-1, 3-dithiine (2), diallyl trisulfide (3) and methyl allyl trisulfide (4)
Garlic ; Sulfur ; Chemistry

In vitro bioavailability of ketoprofen 200mg long acting tablets by the oral were studied. Using basket dissolution tester at 120rpm. Released ketoprofen was determined by a UV – spectrophotometer at 260nm. Ketoprofen plasma levels on carefully chosen 6 healthy volunteers were within treatment which reached from 4 to 36 hours. 200mg ketoprofen LP tablets gradually released ketoprofen within 24 hours and released about 80% after 18 hours. Pharmacological parameter of ketoprofen 200mg long acting tablets have the same value compared with the results which have been published
Biological Availability ; Pharmaceutical Preparations ; Ketoprofen ; tablets ; drugs

A random study on skin temperature change at 12 couples of Yuan point was carried out on 120 subjects. These subjects were divided into two groups: 60 subjects with duodenal ulcer and 60 healthy subjects served as control. Results: There were difference in skin temperature of bilateral Thai xung point in both male and female with gastric-duodenal ulcer (P<0.05-0.01). Temperature of 12 couples of Yuan point in this group was higher than those in control group (P<0.05-0.001)
Peptic Ulcer ; Skin

The fluidextract of PG (2ml contains 1 g radix of PG) was used to evaluate its cardio vascular effects on dog blood pressure, frog isolated intestine. Our experimental results show that: The fluidextract of PG in dose of 0.7g/kg body of weigh (correspond to the clinical dose) per oral route decreases blood pressure by 24.1% in comparison with initial level. PG influences both on the heart frequency and on the heart amplitude depending on the utilizing doses. Low doses have stimulating effect whereas high doses inhibiting one. PG with our experimental doses provoke vasodilatation but don't influence on the intestinal peristalsis
Cardiovascular Agents ; Panax

Studying retrospectively 117 cases of atherosclerosis in the Hospital of Hematology and Blood Transfusion in Ho Chi Minh city showed that: atherosclerosis is very common in elderly and the form of calcified plaque accounts for highest rate. Atherosclerosis is usually associated with other pathological status such as CHA, blood lipid disorder, diabetes and obesity
Blood Coagulation Factors ; Arteriosclerosis ; Blood

Objectives: To evaluate the influence of Ganoderma lucidum on cholesterol drunk - induced hyperlipidemia rats. Methods: Rats were received cholesterol in the oral daily during 8 consecutive weeks in order to hyperlipidemia rats. From 5th week, extract of Ganoderma lucidum (GL) at the doses of 2g/kg, 4g/kg administered orally was investigated in cholesterol drunk - induced hyperlipidemia rats. Results: GL significantly decreased plasma triglycerid, total cholesterol, LDL - C, but increased HDL - C. The effect of GL on plasma lipid level at the oral doses of 2g/kg, 4g/kg was equal to each other and to Lopid (gemfibrozil) at the dose of 140mg/kg. Conclusions: GL have a hypolipidemia effect on rats.
Hyperlipidemias ; Ganoderma ; Cholesterol ; Animal Experimentation

Vnukovo-32 (cell culture rabies virus vaccine strain) was propagated experimentally in primary hamster kidney cell (PHKC), primary Chick embryo fibroblast cell (CEF) and human diploid cell (WI-38). These 3 cell lines were infected with Vnukovo-32 by the same mount of infection (MOl) - lLD50/l000 cells. The result showed that the Vnukovo-32 was adapted on all experimental cell lines, met the criteria of master seed (the titer ranging 6.4-7.5 log LD50/ml), and reached the highest titer on the 7th - 9th day postinfection in three cell groups. Moreover, the highest titer of Vnukovo-32 was met in human diploid cell (WI-38), then in PHCK, and the lowest was in CEF. From these results, it is suitable to use WI-38 to propagate Vnukovo-32 for master and working seed. The procedure for propagation of Vnukovo-32 is set up.
Rabies Vaccines ; Cells