The fluidextract of PG (2ml contains 1 g radix of PG) was used to evaluate its cardio vascular effects on dog blood pressure, frog isolated intestine. Our experimental results show that: The fluidextract of PG in dose of 0.7g/kg body of weigh (correspond to the clinical dose) per oral route decreases blood pressure by 24.1% in comparison with initial level. PG influences both on the heart frequency and on the heart amplitude depending on the utilizing doses. Low doses have stimulating effect whereas high doses inhibiting one. PG with our experimental doses provoke vasodilatation but don't influence on the intestinal peristalsis
Cardiovascular Agents ; Panax

Objectives: To evaluate the influence of Ganoderma lucidum on cholesterol drunk - induced hyperlipidemia rats. Methods: Rats were received cholesterol in the oral daily during 8 consecutive weeks in order to hyperlipidemia rats. From 5th week, extract of Ganoderma lucidum (GL) at the doses of 2g/kg, 4g/kg administered orally was investigated in cholesterol drunk - induced hyperlipidemia rats. Results: GL significantly decreased plasma triglycerid, total cholesterol, LDL - C, but increased HDL - C. The effect of GL on plasma lipid level at the oral doses of 2g/kg, 4g/kg was equal to each other and to Lopid (gemfibrozil) at the dose of 140mg/kg. Conclusions: GL have a hypolipidemia effect on rats.
Hyperlipidemias ; Ganoderma ; Cholesterol ; Animal Experimentation

On white rats, high doses of paracetanol (2g/kg of body mass) and carbonetetrachloride (CCl4 –1.4ml/kg body mass administrated orally) increased dramatically serum transaminase AST and ALT, reduced liver capacity of cholesterol and total protein synthetizations. Granulous degeneration, lipid degeneration and necrosis focus were observed pathohistologically. Linh chi mushroom fluid extracts with the dose of 4g/kg of body mass reversed these biochemical indicators and restored nearly normally the damaged tissues. Similar results were obtained with the oral dose of 25mg Silimarin brand name preparation/kg body mass.
Agaricales ; Reishi ; Liver ;

Background: Like other countries in the world, Vietnam has seen the appearance of many infectious diseases such as SARS, influenza A/H1N1. Therefore, monitoring and quick response to infectious diseases, increasingly require enhancing the capacity of test systems. \r\n', u'Objectives: To determine the capacity for microbiological diagnosis and etiology of communicable diseases at the provincial centers for preventive medicine.\r\n', u'Subjects and methods: With the application of the cross-sectional approach, the study was conducted on microbiology diagnostic capacity for communicable diseases surveillance and response system in 55 Provincial Preventive Medicine Centers (PPMCs)\r\n', u'Results: Laboratory testing and confirmation (isolation and bio-chemical tests, gram stain) are only available for common nitrobacteria such as Escherichia coli, Vibrio cholera, Shigella, Salmonella, and some of the respiratory bacteria as streptococcus, meningococcus, etc... in most PPMCs. ELISA/MACELISA technique for detecting virus pathogen such as Arbo viruses (Dengue, Japanese B encephalitis) and Hepatitis B, HIV is also a focus of these PPMCs. However, for diseases caused by other viruses like Polio, Rota, measles, influenza, PPMCs have only the ability to collect specimens. \r\n', u'Conclusions: Most of the cases reported in the surveillance reports are based on clinical signs, only. Case confirmation was done by hospital/regional or national laboratory. \r\n', u'\r\n', u'
microbiological diagnosis ; provincial centers for preventive medicine

Background: The strategy of Integrated Management of Childhood Illness (IMCI) was developed as an approach/tool for reducing the childhood mortality in developing countries. IMCI was approved by the Ministry of Health of Viet Nam and has been implemented in more than 3500 communal health centers nationwide. Aims: 1) To discover the quality of health care services for children under-5 and the situation of IMCI in health facilities. 2) To propose solutions to improve the quality of health care services for children. Materials and method: The cross-sectional study using quantitative and qualitative approaches conducted 58 direct observations in health workers, interviewed 58 caretakers, comprehensively interviewed 20 local leaders and technical staffs, facilitated 12 focal group discussions and reviewed 120 records of 12 commune health centers. Results: IMCI was considered by all of the participants as a comprehensive approach to improving the quality of childhood health care at first-level health facilities. IMCI contributed towards improved case management skills of health workers, improved the supply of essential drugs and supplies for child health care. As a result, the quality of health services for children under-5 has been promoted (93% of illness children were correctly assessed and classified, 84.6% of them were correctly treated and counseled). Health workers in Ly Nhan district, Ha Nam province adhered to 8.4+/-1.5 out of 10 essential steps of comprehensive child health care. Contrarily, this indicator was low in the districts of Bac Giang province (4.8+/-1.5), not much different to untrained IMCI health workers. Conclusion: IMCI is a useful strategy to improving the quality of child health care. But there were some difficulties that affected the implementation of this strategy in first-level health facilities.
IMCI ; Child health care

Background: DNA biosensor is based on micro-nano technologies aimed at developing a rapid diagnostic device of infectious diseases and diseases related to genetic change. Biosensors are compact size, high sensitivity and low cost\r\n', u'Objectives: To evaluate effect of biosensor in detecting specific gene fragments of Herpes Simplex virus type 1 and 2 (HSV)\r\n', u'Subjects and method: The electrical signals were recognized by means of transducer and from electrochemical detection of the hybridization between the probe 5\ufffd?AT CAC CGA CCC GGA GAG GGA C-3\ufffd?which were covalently immobilised onto the surface of micro electrodessensors in 3-aminopropyltri-ethoxysilance (APTS)-the conducting polymer matrix and the target (specific DNA sequences of HSV in the sample.\r\n', u'Results: The DNA sensor offers a very high sensitivity, a fast response time, less than 1 min with the DNA target concentration up to 1nM in aqueous media at room temperature.However, in order to detect target DNA in the real samples, samples must be extracted DNA, denatured DNA sequences from a double fiber to single fiber. The measurement should be done soon\r\n', u'Conclusion: The results show a large promise to develop quickly DNA sensors for widely application in bio-medical research \r\n', u'\r\n', u'
Biosensing Techniques ; CNBP protein ; human ;

Background: Investment to meet the man power requirements are recognized as urgent; especially to efficiently implement the National Strategy of Preventive Medicine. To strengthen the capability of provincial preventive medicine centers, the Ministry of Health has approved Decision No 05/2006QD-BYT for functions, tasks, authorization and organizational structure of Provincial Preventive Medicine Centers (PPMCs). Objectives: The study was conducted to evaluate the manpower and organization structure of northern PPMCs and provide recommendations for policy makers. Subjects and method: Using the cross-sectional descriptive method, the study covered the preventive medicine centers of 29 northern provinces between Jan to Jun 2007. The information was collected by interviews and self-reported questionnaires. Results:23/29 PPMCs have not met the criteria of man power stated in the Circular 08/2007/TTLB-BYT-BNV. Only 9/29 PPMCs were well organized in accordance with Decision 05/2006/QD-BYT of the Ministry of Health. The average number of staffs in PPMCs was 50+15. Medical staffs accounted for 53%, out of which 21.1% had postgraduate degrees; 32.2% had graduate degrees and 23.3% had been trained in preventive care. Conclusion: To meet the requirements provided by the Decision No05/2006QD-BYT, the man power and training for staffs in PPMCs should be improved and strengthened.
Man power ; Preventive medicine.

Background: In recent years, due to the outbreak of new infectious diseases, re-emerging diseases and bio-terrorist threats, the biological safety for laboratories is essential\r\n', u'Objectives: to evaluate knowledge and practices related to biosafety of researchers in microbiological laboratories\r\n', u'Subjects and method: The study was carried out in the period 2006-2007. Questionnaires and checklists were used for the direct interview the knowledge and observe the practices related to biological safety of 97 laboratory technicians from microbiology laboratories of 22 provincial centers for preventive medicine, which represent for all areas in Vietnam.\r\n', u'Results: The percentage of technicians defines correctly the hazardous groups of some common pathogens are 8.2-33%. The percentage of technicians define correctly the transmission routes of Bacillus anthracis, Staphylococus, Streptococcus are 1%, 15% and 19.6%, respectively. The opinion that thay can wear the laboratory blouse out of laboratories, bring personal belongings into the laboratory and pipeting by mouth are 21.6%, 50.5% and 23.7%, respectively. Regarding laboratory practices: The percentage of technicians does not use gloves is 37.8%; pipeting by mouth: 22.6%. Over 40% technicians do not disinfect working area or washing hands with alcohol after experiments\r\n', u'Conclusion: The results of this study are a basis for planning programs to train, supervise and improve the operational quality of the microbiological laboratory of the provincial preventive health care centers.\r\n', u'\r\n', u'\r\n', u'
Health Knowledge ; Attitudes ; Practice ; Biotechnology/ standards ; Safety/ standards ;

Background: Acute lower respiratory tract infection, mainly pneumonia, were the main reasons cause death for children under 5 years old. Objectives: Determine the isolated rate of bacteria inpatients under 5 years old with acute lower respiratory tract infection in Ha Noi and antibiotic resistance of pneumococcal isolated form patients. Subjects and method: Patients under 5 years old with acute lower respiratory tract infection in National hospital of pediatrics and Bach Mai hospital from 01/2002. Using quantitative culturedand PCR method. Results: Out of total 164 patients with lower respiratory tract infection, there were 91 diagnosed pneumonia by chest X-ray, 73 cases of acute bronchitis. 73,6% of the pneumococcal isolated were penicillin resistance (gPRSP) with different genes such as pbp 1a+2x+ab. Most of the S.pneumoniae strains were serotype 19F or 23F. There were no statistic differences by comparison charactersistics of weight, vessel, subclinical symptoms such as: dissolved oxygen level (S\xac\xacp\xac\xac\xac\xacO\xac2\xac), the amount of leucocyte in blood. However, temperature of pneumonia patients was higher than bronchitis patients, breathing of pneumonia patients was also faster than bronchitis patients. Isolated bacteria with amount \ufffd?106 cfu/ml was H.influenzae, S.pneumoniae and Moraxell catarrhalis in pneumonia group, bronchitis group was 28,8% and control group was 17,1%. Conclusion: Penicillin, erythoromycin and co-trimoxazole resistance rate of S.pneumoniaein patients with acute lower respiratory tract infection was high. Quantitative cultured method has prognostic value in diagnosis pneumonia.
Genes ; MDR/ drug effects ; immunology ; Streptococcus pneumoniae/ growth & ; development ; Anti-Bacterial Agents

Background: Plasma lipids are affected not only by dietary intake and life style but also by genetic factor. Objective: To discover the relationship between plasma lipids and the presence of LPL S447X and PAF-AH V279F in the bodies of rural Vietnamese population. Subject and Method: One hundred and ninety three subjects were randomly selected from rural areas in Ho Chi Minh city. The presence of LPL S447X and PAF-AHV279F polymorphism was determined using an Invader assay, and the plasma lipid levels were measured by an enzymatic method. A profile regarding the dietary intake was also made for each subject. Result and Conclusion: The frequency of LPL 447SX/XX (12.2%) polymorphism, PAF-AH Val279Phe mutation allele frequency (5.4%) in the Vietnamese was closely similar to that of other Asian populations. LPL447X polymorphism was found to be significantly associated with a higher HDL-C level in comparison to LPL 447S. LPL S447X affects plasma HDL-C and TG concentration in Vietnamese rural population.
Genetic factors ; plasma lipids ; rural area