PCR-RFLP and DNA sequencing techniques were used for Pyrimethamine (PYR) and Cycloguanil (CYC) resistant mutations. The study results showed that 46 of 58 Plasmodium falciparum isolates were genotype resistant to pyrimethamine (79.31%). Thirty-one isolates were taken in vitro microscopic examination and revealed that 26 of 31 isolates were PYR resistance (83.87%) and 6 of 31 isolates of CYC resistance (19.35%). The resistant levels of these isolates were related to the mutations in DHFR genes
Plasmodium falciparum ; Pyrimethamine ; Chloroguanide ; malaria

Background: Ha Vi poultry market is a concentrated and spontaneous poultry business place. Ensuring environmental hygiene is not respected.\r\n', u'Objectives: To evaluate infectious situation of airborne fungi at a poultry market\r\n', u'Subjects and methods: From 10/2006 to 9/2007 a survey on the profile of airborne fungi was carried out at the Havi poultry - market. Total fungal spores averaged at 2.753 \xb1 576 cfu/m3, the highest sample is 7.120 cfu/m3\u2022 More than 10 different fungi were isolated. \r\n', u'Results: The common fungi are Cladosporium (30.13%), Penicillium (26.86%), Aspergillus (20.97%, composed of A.niger, A fumigatus, A.jlavus, A.clavatus ...), Fusarium (8.15%), some rare fungi (Rhizopus, Rhizomucor, Mucor, Alternaria, Curvularia, Absidia, Bipolaris ... ) 13.89%. The concentration of airborne fungi at the market was higher than at distance of 300 and 1000 m. The concentrations of total fungi and of each species are affected by some meteorological factors: they were higher in the end of spring, early of summer and autumn, lowest in the winter. The concentrations of Cladosporium, Penicillium were found to be positive correlate with humidity, Aspergillus, Fusarium with temperature. \r\n', u'Conclusion: It is necessary to propose the methods that can reduce influence of airborne fungi pollution to health of people \r\n', u'
Airborne fungi ; Cladosporium ; Penicillium ; Aspergillus ; Fusarium.

Background: Mitral valve diseases are the most frequent in the cardiac valvular acquired diseases. Valvuloplasty is a very good surgical treatment, also its techniques are difficult. Objective: (1) To describe features of valvular lesion and technique of valvuloplasty. (2) To evaluate the effects of these operations. Subject and Method: 95 cases of mitral valve reconstruction were studied and described retrospectively about pre- operative features, mitral valvuloplasty, and post-operative results. Results: Pre-operative cardiac failure >=2 nd level (NYHA) is 96.8%, with 100% of mitral valve insufficiency, 28.4% associated with valvular stenosis. Mean age of patient is 34.5 +/- 13.2. Each case must do an average 4/15 valvuloplasty techniques, the most frequent are: mitral valvular ring (67.4%), commissurotomy (57.9%), cordage section (45.3%)\u2026 Early post \u2013 operative result is very good: no mortality, mitral valvular function is improved significantly than pre-operative (p<0.001), no severe mitral stenosis or insufficiency. Long-term post operative control (3 months, 3 years, 5 years post \u2013 operation) present the stability of this valvuloplasty with high survival frequency (96.8% in 3 months, 96.2% in 3 years, 93.4% in 5 years), in the 78.5% ranges a good level, re-operation is only required in 3 cases with valvular replacement. Conclusions: Although the difficult technique of valvuloplasty \u2013 especially in rheumatic lesion, mitral valve reconstruction is a good and safe technique to treat the mitral valve diseases.
Alkali burn ; Steroid

Background: Flail chest is a severe condition of thoracic trauma, and it requires diagnosis and treatment as soon as possible. From year 2000, we developed an improved technique of open fixation in order to adapt the situations of Vietnam health settings. Objectives: To report preliminary results of application of improved open fixation technique in Viet Duc Hospital from 2001 to 2006. Subjects and method: This descriptive, prospective and retrospective study involved 19 patients with flail chest due to closed thoracic trauma or multiple traumas, treated by open fixation technique. The parameters included features of patients, characteristics of this technique and postoperative progression.Results: Of 19 patients, there were 16 males and 3 females, mean age: 47.8 years. All patients presented obvious signs of flail chest preoperatively, but only 9 cases were treated by open fixation technique and pleural drainage immediately. This technique canbe applied in all sugical settings with single local anaesthesia, and any kind of common surgical threads. The most suitable initial pulled weight is 2000 g. All cases of flail chest required pleural drainage. All patients had good outcomes with open fixation technique, no death. There were 3 patients with postoperative complications, all were severe multiple trauma patients. Conclusion: Improved open fixation technique applied in Viet Duc Hospital is simple, effective and safe, and can be widely used in all surgical settings.
Flail Chest ; Flank Pain/ therapy ;

Background: Chronic obstructive pulmonary disease (COPD) remains a major public health problem. It is the fourth leading cause of chronic morbidity and mortality in the United States, and is projected to rank fifth in 2020 in burden of disease caused worldwide. Objective: To research the epidemiology of COPD in the population of Bac Giang city and finding out the risk factors of COPD. Subjects and method: Cross-sectional survey of general population sample of 2,104 men and women with the age \ufffd?0 years in 30 wards of 11 communes of Bac Giang city. Data on respiratory symptoms, diseases, and risk factors were collected. Lung function tests were performed with Spiroanalyzer snoo, Japan. Results and conclusions: The incidence of COPD was 2.3%; in men 3.0% and in women 1.7%. The incidence of simple chronic bronchitis was 6.4%. People who smoked had higher rate of COPD than the others (OR = 2.8). The incidence of smoking in COPD was 55.9%. The three most important risk factors of COPD was the age \ufffd?0; smoking >15 pack/year and medical history of asthma.
Pulmonary Disease ; Chronic Obstructive/ epidemiology

Background: Human Heparansulphate interacting protein (hHip) has been shown to participate in biological processes of cells. Several studies indicated that hHip transcript is up regulated in several of cancer tissues including those of thyroid, colon, breast and prostate. Antibody against hHIP is necessary for methods to evaluate protein level of HIP in cancer tissues. Objectives:The aims of study was to induce anti hHIP antibody in rabbit and purify and conserve purified anti hHIP antibody. Subjects and method: The study included 9 adult and healthy rabbits with the weight 2 - 2.5kg. Immunization hHIP peptide-KLH in rabbit. Purify anti hHIP antibody using affinity chromatography. Results: The results shown synthesize hHIP peptide and conjugate it with carrier protein. Sensitive rabbit better meet with hHIP-KLH antibody. The Ig concentration obtained in sensitive rabbit was rather high and equal. Immunization hHIP-KLH successfully in rabbit. Obtainment valuable amount of anti hHIP antibody. Conclusion: Successfully induce and purify anti hHIP antibody from rabbit. Establish a standard protocol for polyclonal antibody against small peptide in rabbit.\r\n', u'\r\n', u'
Carrier Proteins/ administration & ; dosage ; chemistry ; Rabbits

Background: Fungal keratitis is a serious ocular infection that can cause corneal scarring and blindness. Currently, diagnosis of fungal pathogens remains a difficult problem. Objectives: To investigate the application of semi-nested PCR targeted ITS genes for detection of fungal agents causing keratitis. Material and method: Ten identified fungal strains, 4 bacterial strains, 20 scraping samples from patients with suspected fungal keratitis and 2 scraping samples from patients with suspected bacterial keratitis were tested using semi-nested PCR. Results: Semi-nested PCR showed positive results for the samples of identified fungal strains and for the 20 scraping samples from patients with suspected fungal keratitis. Neither samples of bacterial strains nor scraping samples from suspected bacterial keratitis patients gave positive PCR results. Conclusion: Semi-nested PCR is a robust tool for specific and rapid detection of fungal agents causing keratitis.
Fungal keratitis ; semi-nested PCR

Objective: The purpose of this study was to identify factors associated with twin pregnancy following day 3 double embryo transfer (DET). Methods: This retrospective cohort study incorporated data from 16,972 day 3 DET cycles. The participants were women aged between 18 and 45 years who underwent in vitro fertilization with intracytoplasmic sperm injection (IVF/ICSI) at My Duc Assisted Reproduction Technique Unit (IVFMD), My Duc Hospital, located in Ho Chi Minh City, Vietnam. Results: Of the 16,972 day 3 DET cycles investigated, 8,812 (51.9%) resulted in pregnancy. Of these, 6,108 cycles led to clinical pregnancy, with 1,543 (25.3% of clinical pregnancies) being twin pregnancies. Factors associated with twin pregnancy included age under 35 years (odds ratio [OR], 1.5; 95% confidence interval [CI], 1.32 to 1.71; p<0.001) and cycles involving the transfer of at least one grade I embryo. Relative to the transfer of two grade III embryos, the risk of twin pregnancy was significantly elevated following the transfer of two grade I embryos (OR, 1.40; 95% CI, 1.16 to 1.69; p<0.001) or a combination of one grade I and one grade II embryo (OR, 1.27; 95% CI, 1.05 to 1.55; p=0.001). Conclusion By analyzing a large number of IVF/ICSI cycles, we identified several predictors of twin pregnancy. These findings can assist medical professionals in tailoring treatment strategies for couples with infertility.

Background: peripheral vascular wounds/trauma is a severe type of surgical emergency, the increasing rate due to the rapid increase of traffic, labor and living accidents. Subjectives and Method: retrospective study, all patients with peripheral vascular wounds/traumas (alone or combination in multiple trauma) were emergency operated at Viet Duc Hospital from January 2004 to June 2006. Results: A total of 310 patients with peripheral vascular injuries in the study, of which accounted for 62.3% of injury, trauma group accounted for 37.7%. The mean age of 30. In first aid, pressed tape accounted for high rate (71.5%). Rate of popliteal vascular trauma due to fractures around the knee accounted for 65.5%, the rate of brachial vascular trauma with fractures around the elbow was 50%. Rate of late diagnosis of arterial trauma was 14.6%, higher than the arterial wounds (2.6%). The rate of ultrasound for forelimbs - where vessels were many wounds, was lower than that for hindlimbs - where vessels were many traumas (44% vs 67.9%). For vascular wounds, more directly vascular connection and more intervention on veins, whereas, for arterial trauma, more vascular graft. Rate of complications was low (7.4%), with no deaths. Conclusions: Overall, the results of emergency surgery for peripheral vascular wounds/trauma were well. No cases was death from vascular lesions, rate of complications was low.
Blood Vessels/ injuries ; Emergencies ;

Objective: This study aimed to investigate the impact of hyperandrogenism (HA) on the outcomes of ovulation induction (OI) using gonadotropin and intrauterine insemination (IUI) in patients with polycystic ovary syndrome (PCOS). Methods: This was a retrospective cohort study including 415 patients undergoing OI using gonadotropin and IUI treatment between January 2018 and December 2020 at a single infertility center. Baseline characteristics, clinical and laboratory parameters, and pregnancy outcomes were investigated. Results: Among the study population, there were 105 hyperandrogenic (25.3%) and 310 non-hyperandrogenic patients (74.7%). The live birth rate was lower in the HA group than in the non-HA group, but this difference did not reach statistical significance due to the limited sample size (14.3% vs. 21.0%, relative risk=0.68; 95% CI, 0.41–1.14, p=0.153). No predictive factors for live birth were identified through logistic regression analysis. Conclusion HA did not negatively affect the outcomes of OI using gonadotropin and IUI cycles in Vietnamese women with PCOS. The result may not be applicable elsewhere due to the large variation in the characteristics of women with PCOS across races and populations.