Acetonitril was used to extract ketoprofen from plasma. The revese phase HPLC was carried out with UV detector at 253nm column Nucleosil ODS 5m (4,6*250 mm); mobile phase: MeCN: AcOH: Triethylamin: H2O (50: 0.5: 0.3: 48.7 v/v); flow rate 0.85 ml/min. Experimental results proved that the method was stable, accurate and precise
Plasma ; Ketoprofen ; Chromatography, High Pressure Liquid

At the Department of internal neurology of Cho Ray Hospital from Dec 2000 to Jan 2003. 130 patients with acute hemisphere cerebral ischemic stroke were studied. Among them, 34 patients could not evaluated by transcranial Doppler sonography, only 79 had transcranial Doppler sonography (TDS) results and MRI results, which confirmed cerebral infarctus. TDS found 30 normal cases, 28 cases with asymmetric indices reduced, 18 cases increased, 1 case of localized acceleration, 2 cases of obstructive cerebral artery in the side of clinical symptom. MRI found 17 normal cases, 62 cases of stricture of cerebral arteries. Sensitivity and specificity of TDS accounted for 74.2% and 82.4% in comparing with cerebro-vascularogramme made by MRI.
Cerebrovascular Accident ; Brain Ischemia ; Cerebral Angiography ; Ultrasonography, Doppler, Transcranial

120 healthy subjects were selected and divided into two groups to study the cutaneous conductivity at 12 couples of Yuan point, group 1 including 60 subjects aged between 21 to 25 (30 males, 30 females), group 2 including 60 subjects aged between 50 to 67 (30 males, 30 females). The results: there was no difference in cutaneous conductivity at 12 couple of Yuan point of the lest and right body in both groups of ages and sexes. In female, cutaneous conductivity at 12 couple of Yuan point were diferent depending on menstrual cycle, considerably lower in the latter haft of the cycle compared to the first half of the cycle. In the same age group, cutaneous conductivity at 12 couple of Yuan point in male was lower than that in female. In the same gender group, cutaneous conductivity at 12 couple of Yuan point of the group 1 was lower that of the group 2
Administration, Cutaneous ; Adult ; Medicine, Traditional

Most of chemical drafts that treatment malignant disease at blood- forming organs have side effects as: reduce aleukemia, glomerule, blood pigment, increase ure, creatinin or nauseate, voltmit, lose hair. However, rate and level are different in each of drafts. The other drafts, that made side effects are Doxorubicin + Ara.C (P3), MOPP (P2), CHOP (P1) and VAD (P4). Ara.C + Depersolon (P7), Hydrea + Ara.C (P6) drafts and especially is only use single Hydrea dafts that has little side-effect
Hematologic Neoplasms ; drug therapy ; Pharmaceutical Preparations

The potency of 33 lots of Japanese encephalitis vaccine produced during the year period 2000-2001 at the Company for Vaccine and Biologicals No1 was evaluated by Shewhart chart. Data showed that no any potency of these lots falls outside the range of one standard deviation 1SD. Results showed the good stability of production procedure, the efficacy of produced vaccine of the manipulation and of the practical conditions
Japanese Encephalitis Vaccines ; Encephalitis

At the National Institute of Pediatrics, the cause of acute respiratory inflammation was studied. Most of pathogenic bacteria isolated from patients with pneumonia and bronchitis were S.pneumoniae (48%) and H.influenzae (44%). The methods using antibiotics dilution on agar to determine the minimum concentration of bacteria and PCR to determine the penicillin resistant gene sequences were used. Results showed that 58% of strains of S.pneumoniae were resistant to penicillin with a sensitivity 19%; 74% resistant to cefotaxin, sensitivity 16%. Other beta-lactamase antibiotics except ampicillin (resistance 35%, sensitivity 30%)
Anti-Bacterial Agents ; bacteria ; Respiratory Tract Diseases

Garlic (Allium sativum L.) belongs to the family Liliaceae. It has been used worldwide for long time as spice and folk medicine. Sulfur-containing compounds from Allium sativum L. are especially noticed because these compounds in Allium sativum L. is significantly higher than those in other plants, and due to its pharmacological activities in sulfur-containing medications such as penicillin and sulfonamide antibiotics. From the liquid obtained by garlic cloves press, four sulfur-containing compounds were isolated. Their structures have been elucidated using MS and NMR spectroscopic methods to be ajoene (1), 2-vinyl-4H-1, 3-dithiine (2), diallyl trisulfide (3) and methyl allyl trisulfide (4)
Garlic ; Sulfur ; Chemistry

Sliding scale insulin was often applied for emergency patients. However there is no study on this therapy in Viet Nam. We used this therapy for 51 new onset hyperglycemic patients (glycemia > 200 mg/dl ) from 6/1998 to 2/2003. Regular insulin was injected when blood glucose>200 mg/dl, then blood glucose level was monitored by finger stick sugar Q4h. If glucose 200-300mg/dl, given 5IU of insulin, glucose>300 mg/dl, given 10 U.The goal blood glucose < 200mg/dl. Results: The average time to lower the blood glucose level to the target level was about 46h. The result after 7 days showed that glycemia was controlled < 200mg/dl and <150 mg/dl in 92% and 62% patientss, respectively.There was no severe hypoglycemia documented during the study.
Insulin ; Therapeutics ; Hyperglycemia ; Stress

In vitro bioavailability of ketoprofen 200mg long acting tablets by the oral were studied. Using basket dissolution tester at 120rpm. Released ketoprofen was determined by a UV – spectrophotometer at 260nm. Ketoprofen plasma levels on carefully chosen 6 healthy volunteers were within treatment which reached from 4 to 36 hours. 200mg ketoprofen LP tablets gradually released ketoprofen within 24 hours and released about 80% after 18 hours. Pharmacological parameter of ketoprofen 200mg long acting tablets have the same value compared with the results which have been published
Biological Availability ; Pharmaceutical Preparations ; Ketoprofen ; tablets ; drugs

Using white Swiss mouse at 3-4 weeks of age for injection of Japanese Encephalitis (JE) virus. Every 8 lots of the mouse had been infected with Nakayama and Beijing-1 strains by injection of 0.03ml into their brains, then followed up within 3 days. The paralysed mice were collected at the 69th hour of the beginning and at the 120th hour of the end. The interval between collection time was every 3 hours. In this study, the rate of mice with paralysis due to Beijing-1 strain was higher than those due to Nakayama strain (94.99% and 92.97%, respectively). The authors suggested that use of Beijing-1 strain to produce JE vaccine is more effective than Nakayama strain.
Japanese Encephalitis Vaccines ; Mice