The present work which was investigated in July 1979, was to observe the wormicidal effects of the external mucous substances of 9 species of fresh water fishes (Cyprinus carpio, Parasilurus asotus, Anguilla japonica, Ophicephalus argus, Carassius carassius; golden crusian carp, Misgurnus anguillicaudatus, Zacco platypus, Pseudorasbora parva and Carassius carassius; crusian carp) on cercaria, liberated metacercaria and adult of Clonorchis sinensis. The mucous substances extracted by ether from the above 9 species of fishes were separated into many spots on the silica gel thin layer chromatography, and divided into many fractions in the silica gel column chromatography which used petroleum ether (30 percent) and chloroform (70 percent) as a solvent. On the silica gel thin layer chromatography of ether extracts from 9 species of fresh water fishes, each of the Rf. values which had wormicidal effect on the cercaria of C. sinensis were different from others; Rf. value of C. carassius (crusian carp) was 0.937, 0.709 in O. argus, 0.612 in A. japonica, 0.576 in P. asotus, 0.451 in C. carpio, 0.701 in Z. platypus, 0.385 in C. carassius(golden crusian carp) and 0.15 in P. parva. Time for wormicide was different from each other, too. It took 14 min. in a case of C. carassius(crusian carp), 25 min. in Z. platypus, 26 min. in C. carassius(golden crusian carp), 28 min. in C. carpio, 30 min. in P. asotus, 35 min. in O. argus, 40 min. P. parva and 180 min. in A. japonica. But any of the spots of M. anguillicaudatus did not show wormicidal effect on the cercaria within 10 hours. In the silica gel column chromatography of ether extracts from 9 species of fresh water fishes, the fractions which had wormicidal effect on the metacercaria of C. sienesis were different from each other; in a case of C. carassius(crusian carp), the first fraction only had wormicidal effect, the 2nd in P. asotus, the 3rd in O. argus, the 4th in C. carassius(golden crusian carp), the 2nd in C. carpio and the 4th in P. parva. Time for wormicide was 40 min. in C. carassius(crusian carp), 52 min. in P. asotus, 74 min. in C. carassius(golden crusian carp), 92 min. in O. argus, 95 min. in C. carpio and 140 min. in P. parva, but any of the fractions of A. japonicas, M. anguillicaudatus and Z. platypus did not show wormicidal effect within 10 hours. The fractions which had wormicidal effect on the metacercaria of C. sinensis had also the same effect on the adult, but it took longer time to kill them. It took 3 hours in a case of C. carassius(crusian carp), 4 hrs. in C. carassius(golden crusian carp), 5 hrs. in P. asotus, 6 hrs. and 6 min. in O. argus, 6 hrs. and 10 min. in C. carpio and half and 6 hrs. in P. parva. But any of the fractions of A. japonica, M. anguillicaudatus and Z. platypus did not show wormicidal effect within 24 hrs.
parasitology-helminth-trematoda ; Clonorchis sinensis ; mucous substance ; Cyprinus carpio ; Parasilurus asotus ; Anguilla japonica ; Ophicephalus argus ; Carassius carassius ; Misgurnus anguillicaudatus ; Zacco platypus ; Pseudorasbora parva

In order to know relationship between the clavate cells of epidermis and infectivity of metacercariae of C. sinensis. histological investigation of epidermis was carried out on the 26 species of fresh-water fish collected from the main rivers in Korea. Results were summarized as it follows: In general clavate cell had a single nucleus at its center. However it was observed in Liobagrus mediadiposalis and Pelteobagrus fuluidraco that clavate cell had two nuclei with and independent so-called light halo of Oxner and in some cases had none of them. In following species; Misgurnus anguillicaudatus, Cobitis taenia, Liobagrus mediadiposalis, Pelteobagrus fuluidraco, Parasilurus asotus and Anguilla japonica, scales were not detected or buried in the dermis layer, and the epidermis was mostly consisted of clavate cells. Among these species, it was observed that no metacercariae were found. In case of Cyprinus carpio, Carassius carassius (crusian carp and golden crusian carp), Sarcocheilichthys czerskii, Pungtungia herzi and Coreoleuciscus splendius, scales were exposed over the epidermis, in which clavate cells were abundantly found. In these species, a few of the metacercariae were detected. On the other hand, no clavate cells were observed in Pseudorasbora parva , Zacco platypus, Microphysogobio koreensis, Gnathopogon majimae, Gnathopogon coreanus, Acheilognathus signifer, Acheilognathus yamatsutae, Cultriculus eigenmanni, Coreoperca herzi, Pseudoperilampus uyekii, Pseudoperilampus notatus and Pseudogobio esocinus. These fishes with exposed scales on the epidermis were well known to serve as the second intermediate hosts of C. sinensis. From the above results obtained, it was suggested that the existance of clavate cells in the epidermis of the fresh-water fish seems to be correlated with the infectivity of the metacercariae of C. sinensis.
parasitology-helminth-trematoda-Clonorchis sinensis ; host ; Liobagrus mediadiposalis ; Pelteobagrus fuluidraco ; Misgurnus anguillicaudatus ; Cobitis taenia ; Liobagrus mediadiposalis ; Pelteobagrus fuluidraco ; Parasilurus asotus ; Anguilla japonica ; Cyprinus carpio ; Carassius carassius ; Sarcocheilichthys czerskii ; Pungtungia herzi ; Coreoleuciscus splendius ; Pseudorasbora parva ; Zacco platypus ; Microphysogobio koreensis ; Gnathopogon majimae ; Gnathopogon coreanus ; Acheilognathus signifer ; Acheilognathus yamatsutae, Cultriculus eigenmanni ; Coreoperca herzi ; Pseudoperilampus uyekii ; Pseudoperilampus notatus ; Pseudogobio esocinu