Objective To investigate clinical and MRI features of the reversible posterior encephalopathy syndrome (RPES).Methods Clinical and MRI materials of 4 RPES cases confirmed by integrated clinical diagnosis were retrospectively analyzed.Results All the cases presented with a rise of blood pressure.The neural symptom include severe headache (2 cases),dysphoria (3 cases),blur of vision (2 cases),impairment of impermanent memory (1 case),seizure (2 cases).In MRI,the lesions mainly systematically located in posterior parietal lobe and occipital lobe in all cases,or randomly located in frontal lobe (2 cases).Lesions demonstrated high signal on T_2 weighed and water-suppressed sequences in MRI.The lesions dominantly located in cortex in 2 cases,in both cortex and subcortieal white matter in 1 case and in subcortical white matter in 1 case.Four cases resolved their lesions when re-examined. Conclusions RPES represents such characteristic MRI findings as symmetrical distribution of reversible lesions in posterior brain with involvement of cortex or subcortieal white matter.When combined with hypertension and neural symptoms caused by involvement of posterior brain structure,it is helpful for early differential diagnosis of this syndrome.

OBJECTIVE:To establish a method for the simultaneous determination of chlorogenic acid,hesperidin and baicalin in Shubiaoling granule. METHODS:HPLC was performed on the column of SAGA Tri-Sal C18 with mobile phase of acetonitrile-0.5%phosphoric acid(gradient elution)at a flow rate of 1.0 ml/min,detection wavelength was 320 nm,column temperature was 30℃,in-jection volume was 10μl. RESULTS:The linear range was 2.463-240.5μg/ml for chlorogenic acid(r=0.999 9),6.577-642.3μg/ml for hesperidin(r=0.999 8)and 1.708-166.8 μg/ml for baicalin(r=0.999 8);RSDs of precision,stability and reproducibility tests were no more than 1.8%;recoveries were 97.02%-102.40%(RSD=1.9%,n=6),96.55%-100.20%(RSD=1.6%,n=6) and 97.51%-100.70%(RSD=1.2%,n=6),respectively. CONCLUSIONS:The method is simple,accurate with high sensitivity and good reprosucibility,and can be used for the simultaneous determination of chlorogenic acid,hesperidin and baicalin in Shubiaoling granule.

The detection of blood flow velocity has great significance for blood vessel monitoring and the research of cardiovascular pathogenesis. Blood flow velocity measurement based on ultrasonic is becoming more and more popular in doctors and patients compare to several other techniques in nowadays, as they are non-invasive, cheap and fast. Most of the traditional ultrasonic blood flow velocity measurement methods are based on the Doppler frequency shift, but theses methods have some limitations, such as angle dependence, limited spatial resolution and so on. Therefore, blood flow velocity techniques based on non-Doppler frequency shift also get rapid development in recent years. This article mainly summarizes the techniques of blood flow velocity estimation based on ultrasonic in these two aspects.
Blood Flow Velocity ; Humans ; Ultrasonography

Objective: To measure the level of T-lymphocyte subsets and immunoglobulin in severe traumatic brain injury and to explore the clinical significance. Method: 48 cases with severe traumatic brain injury were measured in T-lymphocyte subsets and immunoglobulin of vein blood before and after the rehabilitation therapy. By comparing with the control group and combining clinical evaluation to analyze the immune functional state in the state of TBI and the prognosis. Result:The cellular and humoral immune were inhibited distinctly. The level of CD3 + ,CD4 + ,CD4 +/CD8 +, IgG and IgA of TBI group were lower than the level of the control group. In prognosis, the early immune state of severe disability+vegetable state group was inhibited more seriously than good recovery + mild disability group. Conclusion: The cellular and humoral immune of severe traumatic brain injury were in inhibited state. The severe inhibition of immune, the worse the prognosis.

ROS-mediated oxidative stress involved in a variety of cellular signal transduction, FOXO3a transcription factor is an intersection in regulating a variety of cellular oxidative stress. FoxO3a has been extensively studied in regulating oxidative stress because of its rather complex and pivotal regulation of cell proliferation, cell cycle arrest, ROS scavenging and apoptosis. This review will elucidate the FOXO3a’s regulatory mechanisms and describe the target genes involved. It will also provide the clinical significance and strategies to target FOXO3a to regulate oxidative stress.

BACKGROUND:In order to overcome the shortcomings of single materials, antibiotics-loaded hydroxyapatite/titanium composites have attracted people’s attentions.
OBJECTIVE:To evaluate the biocompatibility of vancomycin/hydroxyapatite/titanium nanotubes.
METHODS:Mouse osteoblasts, MC-3T3-E1, were co-cultured with titanium (Cp-T), TiO2nanotubes, and vancomycin/hydroxyapatite/titanium nanotubes, respectively. Cel morphology and growth were observed after 1, 3 and 5 days of co-culture under inverted microscope and scanning electron microscope. The cel proliferation was detected by AO-EB method. The total protein, calcium and alkaline phosphatase levels were detected at 7 and 14 days of co-culture.
RESULTSAND CONCLUSION:The MC-3T3-E1 cels with good viability and morphology adhered wel on the surface of vancomycin/hydroxyapatite/titanium nanotubes compared with those on the surface of pure titanium and TiO2nanotubes under the scanning electron miscroscope. Moreover, there were a large amount of pseudopodia on the surface of composite nanotubes. Compared with the other two groups, the cel number on the surface and the levels of intracelular calcium and alkaline phosphatase were al higher in the vancomycin/hydroxyapatite/titanium nanotubes group. These findings suggest that the vancomycin/hydroxyapatite/titanium nanotubes have good biocompatibility and no cytotoxicity.

Objective To evaluate the prognostic significance of lymph node ratio (LNR) in axillary lymph node positive breast cancer.Methods Eight hundred and three cases axillary lymph node positive breast cancer patients without distant metastasis were systematically treated in the Obstetrics and Gynecology Hospital of Fudan University from 2006 to 2014,at least 10 lymph node removed in each case.Clinicopathological data including 5-year disease-free survival rate (5y-DFSR) and 5-year overall survival rate (5y-OSR) were described.Factors related with prognosis were analyzed by univariate analysis.Prognostic difference was compared among different LNR stage in each axillary lymph node pathological stage(pN).Prognostic significance of pN and LNR was compared by multivariate analysis.Results Mean lymph nodes removed were 15.47±4.70 lymph,and median positive lymph nodes were 4 lymph in 803 cases axillary lymph node positive breast cancer patients.Altogether 159 cases of local recurrence and distant metastasis and 99 cases of breast cancer-related death occurred during median follow-up of 61 months.Five-year DFSR was 77％ and 5y-OSR was 83％.Log-rank univariate analysis showen that pT,pN,LNR,lymphovascular invasion and ER status were related to DFS and OS.Five-year DFSR and OSR for pN1,pN2,pN3 were 89％,68％,59％ and 93％,78％,63％,respectively,whereas 5y-DFSR and 5y-OSR for LNR1,LNR2,LNR3 were 90％,69％,56％ and 94％,80％,57％,respectively.There was statistically significant difference among different LNR in pN1 and pN2 (pN1:DFS:P=0.005,0S:P=0.024;pN2:DFS:P=0.017,0S:P=0.000),but not in pN3,inspite of difference tendency (DFS:P =0.165,OS:P =0.075).In multivariate analysis,when pN or LNR were entered into the Cox regression mode respectively,both were the independent prognostic factors of DFS(P＜0.001) and OS(P＜0.001).When pN and LNR were entered into the Cox hazard regression model at the same time,LNR remained as the independent prognostic factor of DFS and OS (P ＜ 0.001),but pN lost significance (DFS:P =0.461,OS:P=0.162).Conclusion LNR is independent prognostic factor for positive axillary lymph node breast cancer.

Objective To evaluate the clinicopathological characteristics of mucinous breast carcinoma (MBC) and its prognosis.Methods 112 cases of MBC treated in Obstetrics and Gynecology Hospital of Fudan University between Jan 2005 and Dec 2014 were enrolled retrospectively together with 1 157cases of invasive ductal carcinoma (IDC) for comparison.There were 71 cases of pure MBC (PMBC) and 41cases of mixed MBC (MMBC).PMBC and MMBC were compared with each other,and were also compared with IDC respectively.Results PMBC had smaller tumor mass,higher expression rate of hormone receptors (all P＜0.05),lower rate of lymph node metastasis (7.0％ vs.40.6％,x2 =32.663,P ＜0.001) when compared with IDC.The 5 year disease free survival (DFS) and overall survival (OS) of PMBC were both better than those of IDC (DFS:94.6％ ±3.0％ vs.81.3％ ± 1.1％,x2 =7.265,P =0.007;OS:92.4％ ±5.3％ vs.88.5％ ± 1.0％,x2 =4.059,P =0.044).MMBC had relatively larger tumor mass,higher expression rate of hormone receptor,but had no difference in the rate of lymph node metastasis (48.8％ vs.40.6％,x2 =3.417,P =0.332) when compared with IDC.There was no statistically significant difference in 5 yearDFSandOSbetweenMMBCandIDC (DFS:79.1％ ±7.1％ vs.81.3％±1.1％,x2 =0.167,P=0.683;OS:84.5％ ±7.2％ vs.88.5％ ± 1.0％,x2 =0.123,P =0.726).PMBC had relatively smaller tumor mass,lower rate of lymph node metastasis,but had no difference in the expression rate of hormone receptors.The 5 year DFS and OS of MMBC were both better than those of MMBC (DFS:94.6％ ± 3.0％ vs.79.1％±7.1％,x2 =6.772,P =0.009;OS:92.4％ ±5.3％ vs.84.5％ ±7.2％,x2 =6.401,P=0.036).Lymph node status was the only statistically significant prognostic factor of MBC by COX multivariate analysis.Conclusions PMBC has better prognosis than MMBC and IDC owing to its lower rate of lymph node metastasis.MMBC has higher rate of node metastasis than PMBC,hence similar prognosis with IDC.

BACKGROUND: Present studies have proved that titanium coating nanotubes cannot only promote the biological activity of the material itself, but also be used as a drug carrier loading antibiotics and growth factors. OBJECTIVE: To investigate the antibacterial properties of vancomycin/hydroxyapatite/titanium dioxide nanotubes in vitro and in vivo. METHODS: The releasing property in vitro of vancomycin/hydroxyapatite/titanium dioxide nanotubes and vancomycin/titanium dioxide nanotubes were detected. And 1010/L Staphylococcus aureus dilution was put onto the commercial titanium, titanium dioxide nanotube and vancomycin/hydroxyapatite/titanium dioxide nanotube, respectively. Twenty-four hours later, the bacterial growth and activity was observed by scanning electron microscope and confocus scanning electron microscope, respectively. RESULTS AND CONCLUSION: Scanning electron microscope showed: the number of Staphylococcus aureus was the least on the vancomycin/hydroxyapatite/titanium dioxide nanotube, and the bacterial morphology was destroyed. Confocus scanning electron microscope observed: the number of bacteria and viable bacteria was the least on the vancomycin/hydroxyapatite/titanium dioxide nanotube, and the most on the commercial titanium. Besides, the releasing time of vancomycin from the hydroxyapatite/titaniumdioxide nanotube was up to 18 days, but the releasing time of vancomycin was only 4 hours from the titanium dioxide nanotube. In conclusion, the vancomycin/hydroxyapatite/titanium dioxide nanotube has good antibacterial property and slow-releasing performance.

Objective To monitor the association between the expression of ve-cadherin and mesentery microcirculation blood flow of sepsis CASP rats,Analysis the relationship of each other.Methods Based on the standard criteria,we established sepsis CASP rats,and the rats were divided into several groups according to the gauge of the vein detained needle in the ascending colon,every group has six rats.The groups consisted of Sepsis A group 22 G(0.9 mm× 25 mm,33 ml/min),sepis B group 20 G(1.1 mm ×32 mm,54 ml/min),sepsis C group 18 G(1.3 mm ×32 mm,80 ml/min),sepsis D group 14 G(2.0 mm ×45 mm,270 ml/min),in addition we established the control group.Real-time observation was performed by microscope on the 6 h's blood velcocity of each sepsis CASP rats group.Then we applied the immunohistochemistry to quantitatively analyze the expression of ve-cadherin of each group.Results The blood velcocity of the mesentery microcirculation in control group was (583.21 ±52.39) μm/s,it was higher compared with the sepsis D group(213.30 ±52.39) μm/s (P ＜0.05),the blood velocity in sepsis A group was (482.71 ± 58.62) μm/s,higher compared with the sepsis D group(P ＜ 0.05).The score of the immunohistochemistry quantitative analysis of mesentery ve-cadherin in control group was 11.17 ±0.34,higher compared with the sepsis D group(5.43 ±0.43)(P ＜0.01).The score of sepsis A group was 10.07 ±0.30,higher compared with the sepsis D group(P＜0.05).Conclusions The expression of ve-cadherin of sepsis CASP rats had positive correlation with the blood velocity of the mesentery microcirculation of sepsis CASP rats,and it can indirectly reflect the degree of the sepsis.