Objective To evaluate the feasibility and clinical efficacy of laparoscopic biliary reoperation. Methods From May 2000 to May 2007, 105 patients received laparoscopic biliary reoperation, including choledocholithotomy and T-tube drainage in 96 cases, Roux-en-Y anastomosis of the gallstone/hepatic-duct in 5, and liver resection in 4. Results The operations were completed in all the 105 patients without severe complications. The operation time was 60-185 min [mean, (125.3?23.2) min]. Intraoperative blood loss was 15-310 ml [mean, (21.1?8.6) ml]. The patients stayed at hospital for 5-9 days after the operation. The 5 patients, who underwent Roux-en-Y anastomosis of the gallstone/hepatic-duct, were followed up for 6 to 65 months [mean, (13.2?8.6)months]. Partial liver resection was performed in 2 cases (resection of the left hepatic lobe in 2, and the right lobe in 2); they were followed up for 8-25 months [mean, (10.2?3.6)months]. In the 96 patients, who received laparoscopic choledocholithotomy and T-tube drainage, the follow-up were lasted for 3-72 months [mean, (13.2?9.6)months]. None of the patients who were followed up had recurrence of gallbladder stone. Conclusions Laparoscopic biliary reoperation is feasible and safe with minimal trauma to patients.

Objective-To-discuss-the-value-of-fast-track-surgery-(-FTS-)-in-perioperative-period-of-laparoscopic-cholecystectomy-.-Methods-We-selected-200-cases-of-laparoscopic-cholecystectomy-in-our-hospital-from-January-2012-to-December-2012.The-cases-were-randomly-divided-into-either-traditional-group-(control-group)-or-fast-track-surgery-group-(FTS-group),-with-100-cases-in-each-group-.The-operation-time-,-intraoperative-blood-loss-,-time-to-get-out-of-bed-after-operation-,-time-to-intake-semi-liquid-diet,-time-to-flatus,-time-to-defecation,-and-length-of-hospital-stay-were-compared-.-Results-Compared-with-the-control-group-,-the-FTS-group-had-shorter-time-to-semi-liquid-diet-[(11.3-±2.0)-h-vs.(50.2-±8.7)-h,-t=-43.976,-P=0.000],-shorter-ambulation-time-[(6.2-±1.5)-h-vs.(14.3-±1.7)-h,-t=-35.728,-P=0.000],-shorter-flatus-time-[(12.0-±4.4)-h-vs.(24.9-±5.4)-h,-t=-18.519,-P=0.000],-shorter-defecation-time-[(15.8-±5.3)-h-vs.(25.2-±4.3)-h,-t=-13.773,-P=0.000],-and-shorter-postoperative-hospital-stay-[(3.2-±1.3)-d-vs.(4.2-±2.1)-d,-t-=-4.048,-P=0.000].-Conclusion-Fast-track-surgery-is-feasible-and-safe-,being-helpful-for-patient-’-s-postoperative-recovery-.

Objective To study the feasibility of the ultrasonic scalpel in the management of cystic blood vessels during laparoscopic cholecystectomy(LC). Methods Abdominal blood vessels of two dogs were managed by ultrasonic scalpel under the conditions of two step output power,low tension,low holding force and blunt scalpel head.The blood vessels were sealed and arteries and veins of (1～11)mm were cut.On this basis,the technique was applied to the management of cystic vessels during laparoscopic cystic and biliary operation. Results The sealing and cutting effect was good for the blood vessels of less than 9mm 140/150(93 3%).No blood oozing was found at the cutting ends of blood vessels during and after operation.In all 706 cases of laparoscopic gallbladder and biliary duct operations,no bleeding was found at the cutting ends of blood vessels during and after operation. Conclusions Ultrasonic scalpel can be directly used to seal and cut cystic artery during laparoscopic cholecystectomy.

Objective To summarize the experience on the prevention of severe complications of endoscopic thyroidectomy.Methods Twelve cases of bilateral lesions underwent endoscopic thyroidectomy via precordial approach and 41 cases of unilateral lesions, via subaxillary approach. A subcutaneous channel, with a width of about 5 cm from the incision site to the thyroid, was made by using self-made instruments. The CO 2 pressure was set at 4 mm Hg. By ultrasonic scalpel the lesions were divided and removed. Results There were 41 cases of thyroid adenoma (unilateral, 34 cases; bilateral, 7 cases) and 12 cases of nodular goiter (unilateral, 7 cases; bilateral, 5 cases). Postoperative subcutaneous hematoma occurred in 1 case and was cured by conservative management. No nerve damage or parathyroid complications were observed. Conclusions Application of ultrasonic scalpel and continued low cavity pressure are effective means for preventing complications in endoscopic thyroidectomy.

Objective To sum up the experience of laparoscopic assisted radical resection for colorectal cancer. Methods Radical resection was operated on 40 patients with colorectal cancer under laparoscope. According to the Dukes’ classification, 21 patients were classified as stage A, 16 patients as stage B and 3 patients, stage C.There were 2 cases of cecum cancer, 4 cases of ascending colon cancer, 13 cases of sigmoid colon cancer, and 21 cases of rectum cancer. Results Laparoscopic assisted operations were accomplished in all the 40 patients. The operation time was 110.8?23.5 min and the intraoperative blood loss was 150.4?23.2 ml. The patients began to ambulate 12.2?3.8 hours after the surgery and the time to first passing flatus was 39.3?4.2 hours. Follow- up for 2～72 months (mean, 38.5 months) found no metastasis, local recurrence, and port or incision implantation in all the patients but one,who refused chemotherapy and had a cancer recurrence one year after surgery. Conclusions Laparoscopic radical resection is applicable to colorectal cancer at Dukes’ stage A, B, or C, in which the lymph nodes can be removed as thoroughly as open radical surgery.

Objective To explore the application value of hand-assisted laparoscopic splenectomy and pericardial devascularization in the treatment of liver cirrhosis and portal hypertension. Methods Hand-assisted laparoscopic operations of splenectomy in 10 patients and pericardial devascularization in 13 patients were completed by using the LigaSure device and harmonic scalpel. Results All the operations were completed successfully under laparoscope. Of splenectomy, the operative time was 63?15.5 min and the estimated blood loss was 32.4?21.2 ml. Of pericardial devascularization, the operative time was 115.3?25.5 min and the estimated blood loss was 52.4?24.2 ml. All the patients got out of bed and moved around at 12.2?3.8 h after operation. Conclusions [WTBZ]Hand-assisted laparoscopic splenectomy and pericardial devascularization using the LigaSure device and harmonic scalpel has advantages of high reliability, little blood loss, and quick postoperative recovery. The procedure can be used in the management of portal hypertension and hypersplenism.

Objective To investigate expression of histone deacetylase 5 (HDAC5) in normal and colorectal cancer (CRC) tissues and its mechanism.Methods Immunohistochemistry was used to detect the expression of HDAC5 in CRC tissues.The relationship between HDAC5 expression content and survival prognosis was analyzed.Proliferation,immigration and apoptosis changes were observed after knockdown of HDAC5.Results Positive expression of HDAC5 in tumor tissue was related with poor prognosis of CRC patients,and HDAC5 could inhibit the LS174T cell proliferation by regulating the SIX1 expression.Conclusion Our results reveal that HDAC5 expression is variant in CRC tissues,and it is correlated with prognosis of colorectal cancer,and can affect cell proliferation by regulating the SIX1 expression.

Objective To investigate expression of histone deacetylase 5 (HDAC5) in normal and colorectal cancer (CRC) tissues and its mechanism.Methods Immunohistochemistry was used to detect the expression of HDAC5 in CRC tissues.The relationship between HDAC5 expression content and survival prognosis was analyzed.Proliferation,immigration and apoptosis changes were observed after knockdown of HDAC5.Results Positive expression of HDAC5 in tumor tissue was related with poor prognosis of CRC patients,and HDAC5 could inhibit the LS174T cell proliferation by regulating the SIX1 expression.Conclusion Our results reveal that HDAC5 expression is variant in CRC tissues,and it is correlated with prognosis of colorectal cancer,and can affect cell proliferation by regulating the SIX1 expression.

OBJECTIVE: To develop a noninvasive method for prediction of 1p/19q codeletion in diffuse lower-grade glioma (DLGG) based on multimodal magnetic resonance imaging (MRI) radiomics. METHODS: We collected MRI data from 104 patients with pathologically confirmed DLGG between October, 2015 and September, 2022. A total of 535 radiomics features were extracted from T₂WI, T₁WI, FLAIR, CE-T₁WI and DWI, including 70 morphological features, 90 first order features, and 375 texture features. We constructed logistic regression (LR), logistic regression least absolute shrinkage and selection operator (LRlasso), support vector machine (SVM) and Linear Discriminant Analysis (LDA) radiomics models and compared their predictive performance after 10-fold cross validation. The MRI images were reviewed by two radiologists independently for predicting the 1p/19q status. Receiver operating characteristic curves were used to evaluate classification performance of the radiomics models and the radiologists. RESULTS: The 4 radiomics models (LR, LRlasso, SVM and LDA) achieved similar area under the curve (AUC) in the validation dataset (0.833, 0.819, 0.824 and 0.819, respectively; P>0.1), and their predictive performance was all superior to that of resident physicians of radiology (AUC=0.645, P=0.011, 0.022, 0.016, 0.030, respectively) and similar to that of attending physicians of radiology (AUC=0.838, P>0.05). CONCLUSION Multiparametric MRI radiomics models show good performance for noninvasive prediction of 1p/19q codeletion status in patients with in diffuse lower-grade glioma.
Humans ; Magnetic Resonance Imaging ; Chromosome Aberrations ; Area Under Curve ; Glioma/genetics* ; ROC Curve