1.DNA Sequence Analysis of HPV type 33 in the Genital Tract of Woman
Anarkhuu.B ; Battogtokh.Ch ; Bayarmaa.Es ; Banyar Than Naing ; Atsushi Watanabe ; Takashi Shimada
Innovation 2008;5(1):49-52
Our present study investigated DNA sequence analysis of Human Papillomavirus (HPV) typc-33 in sexually active women.
In present study 22 HPV-33 positive Endocervical specimens were obtained by use of Polymerase Chain Reaction (PCR), from total 500 participants, and further analyzed by DNA sequencing of the Long Control Region (LCR), E6 and E7 genes. For the genes LCR and E6 13 samples, for the gene E7 all 22 HPV-33 positive samples were sequenced by Applied Biosystcms.
All 22 HPV-33 positive participants were Mongolian nationality. Most common Non-prototype-Likc variant in LCR is HPV-33 LCR-17 (11/13). one HPV-33 LCR-5 (1/13), and only one Prototype was found (1/13). In the E6, 12 samples were variant 33-E6-6 (12/13), and one prototype was found. lntheE7, 13 Prototype (13/22), 11 Non-prototype-Like variants were found. From the sequence result of gene sites in LCR, E6 and E7 most common HPV-33 variant in Mongolia is MN-17-6-0 (10/13), HPV-33 MN-0-6-0 (1/13), HPV-33 MN-5-6-0 (1/13) and one Montreal variant were found HPV-33 MT-17-0-0 (1/13).
We identified 3 new variants of 11PV-33 which we called MN (Mongolia). From sequence result in 3 sites of genes, LCR is more variable compare with E6 and E7. F.6 were variable compare with E7.
' ' Health Sciences University of Mongolia, Ulaanhaatar, Mongolia 4,6 Department of Biochemistry and Molecular Biology, Nippon Medical School, Tokyo. Japan
2.Comparison of Helicobacter pylori Virulence Factors, Intcrleukin-1 Polymorphisms ni Patients and Endoscopic and Histological I-indiums of Stomach and Duodenum.
Bayasgalan.P ; Anarkhuu.B ; B. Munguntsetseg ; Ochbadrakh.B ; Bolormaa.E ; Maslov. V.E ; Narantsetseg.B ; Narmandali.B ; Gan-Orshih.L ; Ovunchimeg.J ; Tserenlham.S ; MunhjargalJ ; Enh-Ulzii.M ; Enh-Amar.A ; Oyuntsetseg.Kh ; Bira.N ; Suvd.B ; Khoshayar.T
Innovation 2009;6(2):72-77
BACKGROUND
The aim of the study is to detect and define the role of H. pylori virulence factors and host IL-1 polymorphisms to prevent from further gastric cancer.
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5ml of blood samples were collected from each of 42 patients who had abdominal complaint, after informed consent was obtained. All patients were Mongolian nationality. The biopsy specimens were stored in liquid nitrogen and homogenized before DNA isolation. After tissue lysation with proteinase K. DNA isolation was performed with "Promege" tissue kit. according to the manufacturer's instructions. PCR amplification of H. pylori gene loci was performed for the cagA gene and the vacAs mosaics vac As 1 and vacAs2.
RESULTS
Result of histological findings shows 84.7% from all patients were diagnosed with II. pylori infection 83% (35/42). Histologically LI'G 50% (42/21). Gastric atrophy 30% (42/13). Intestinal metaplasia 9% (42/4). Gastroduodcnal ulcer 4% (42/2), Dys¬plasia 11% (42/5), Adinocarcinoma 2% (42/1), 3 patients (42/3, 7%) were none patho¬logic change. 62% (26/42) patients infected with H. pylori, as determined by Urease test. H. pylori were investigated in all 42 patients and 83% (35/42) were infected with II. pylori, as determined by histology (haematoxylin- eosin and (iiemsa-stained). Strain characteristics of H. pylori were investigated in all 42 patients and 83% (35/42) were infected with //. pylori, as determined by UreC PCR.
Result of histological findings revealed Bacilla form 48,5% (17/35), Coccoid form 28,5% (12/35), mixed form 14% (5/35) from all patients were found //. pylori. 76% (13/17) of all patients were revealed coccoid form of H. pylori were taken anti-//. pylori treatment.
The vacAs 1 genotype was found in 38% (16/42) of all UreC+ patients, and cagA was found in 23% (10/42) of UreC+ patients. 16.9% of all patients were IL-RN*2 positive (7/42), (IL-1B 31C/51 IT).