Objective To increase the recognition of the clinicians and laboratorians to hereditary spherocytosis for reducing the misdiagnosis and missed diagnosis ,and improving the diagnostic level .Methods The data in 4 cases of definitely diagnosed heredi‐tary spherocytosis in our hospital were retrospectively analyzed ,and the misdiagnosis reasons were analyzed and summarized .Results In 4 cases ,2 cases were misdiagnosed as Mediterranean anemia ,1 case was misdiagnosed as hemolytic anemia and 1 case as hemo‐globin disease .Conclusion Correctly mastering the key points of the diagnosis ,strengthening the communication between the labo‐ratorians and clinicians ,paying attention to the red blood cells morphologic examination under microscope and high level diagnosis a‐bility of blood cell morphology are the important means to reduce the misdiagnosis and missed diagnosis and improve the diagnostic accuracy rate .

Objective To study the effect of calcium dobesilate on serum Hs-CRP and TNF-α and other micro-inflammatory in patients with maintenance hemodialysis. Methods 74 patients with chronic renal failure undergoing maintenance hemodialysis from April 2015 to December 2016, were randomly divided into the control group(n=39) and the observation group(n=35). All patients are in line with the diagnostic criteria of the guidelines for the diagnosis and treatment of chronic renal failure, established by the Chinese Academy of traditional Chinese Medicine. All patients in the two groups were given atorvastatin, at this basis, the observation group was given calcium dobesilate. After treatment, hypersensitive inflammatory markers, such as C-reactive protein (hs-CRP), tumor necrosis factor -α (TNF-α), as well as nutrient index plasma albumin (ALB) and the pre-albumin (PA) level in the two groups were compared. Results The hs-CRP, TNF-α in the observation groupwere lower than that in the control group, the differences was statistically significant (P<0.05). The ALB, PA in the observation group was significantly higher than that in the control group, the differences was statistically significant (P<0.05). Conclusion Atorvastatin combined with calcium dobesilate was used in the patients with maintenance hemodialysis which can improve thenutritional status and inflammatory conditions, it is safety and is worth popularizing,.

With the development of the medical imaging and computer aided technology,the trend of the medical imag es visualization from2D to3D is focused.This paper introduces the methods and ke y technology of3D medical images visualization,and discusses its applications to medical diagnoses and clinic therapy.There is a promising direction in the fiel ds of virtual endoscope,computer assisted operation and long-distance operation.

Objective To analyze the recent changes in features of joint injury caused by the fast running railroad train. Methods 1279 joint injuries o f the 10214 cases of train injury after the speeding up of the railway services from 1997 to 2003 were analyzed to find out their particular causal conditions, the severity of the injury, and the classification of the injuries, the correlat ion between which and recovery was also tested by AIS-ISS grading. Results Afte r the speeding up of the railway services, the incidence of joint injuries rose from 33.54% to 34.12%,the mortality rose from 22.88% to 30.33%,the multipl e joint severances rose from 19.84% to 34.19%,the open joint injury rose from 31.71% to 63.15%,and the mortality caused by multiple joint severances rose from 21.19% to 49.07%. Conclusions The incidence of multiple traumas resulted from the train is much higher than those resulted from other causes. Multiple t raumas caused by train are very difficult to deal with. The multiple organ dysfu nction syndrome (MODS) is a main factor to cause the death.

Seventy C57BL/6 mice were divided into control group,lard group,lard transferred into safflower oil group,and safflower oil group.After 20 weeks,the white adipose tissues were taken to analyse the gene differentiational expression prolife.The results showed that body weight,blood glucose and lipids,and insulin levels in lard group were higher than those in control group( all P＜0.05 ),which were decreased after lard was transferred into safflower oil for 10 weeks( all P＜0.05 ).Safflower oil regulated some of the orexigenic genes,anorectic genes,and the genes involved in energy expenditure in adipose tissues such as opioid receptor,glucagon,and PPARα.

ObjectiveTo investigate the effect of weight self-management in reproductive-aged obese women with polycystic ovary syndrome(PCOS).Methods A total of 56 reproductive-aged obese women with PCOS were divided into two groups with the control group administered medicine to reduce the lipid,blood glucose and metformin and the experiment group managed with weight self-management in addition of the medicine treatment as in the control group.All of them were treated for six months.The number of women with oligomenorrhea,body mass index(BMI),waist-hip ratio(WHR),serum total cholesterol(TC),triglyceride(TG),fasting blood glucose(FBG), fasting insulin(FINS),homeostasis model assessment(HOMA)were measured before and after intervention for comparisons.Result The number of women with oligomenorrhea together with the levels of TC,TG,FBG,FINS and HOMA were significantly reduced in the two groups(allP<0.05),and the number of women with oligomenorrhea and the levels of BMI,TC and HOMA were decreased more than those in the control group(allP<0.05).Conclusion The weight self-management on the basis of drug therapy for reproductive-aged obese women with PCOS can significantly reduce the levels of BMI,WHR,TC,TG,FBG,FINS and HOMA,enhance the effect of drug therapy, reduce the incidence of oligomenorrhea and improve the metabolic disorders.

The selective toxicity of the mitochondria-specific cationic fluorescent dye rhodamine 123 (Rh-123) on HeLa cells in culture was studied. In this report, we demonstrate that with continuous exposure, Rh-123 markedly inhibited the growth of HeLa cells but had little effect on normal human kidney fibroblasts. With continuously exposed to Rh-123, the growth rate, colony forming ability, and mitotic index of HeLa cells were decreased. The mechanism of toxicity of Rh-123 on HeLa ceils was investigated by EM and enzyme cytochemistry stain. The mitochondria of carcinoma cells were the main targets for the inhibitory action of Rh-123, since they selectively accumulated the dye. At the dosage of Rh-123 which was toxic to HeLa cells, the structure and function of mitochondria were disrupted, as the mitochondriarelated enzymes, i. e., ATPase, LDH, and SDH were inhibited. The possible mechanism of the action of Rh-123 on HeLa cells is briefly discussed.

It expounds the knowledge of causa morbi and mechanism of insomnia, viewing that it is the result of complex reasons functioning to the body, its attack is to some extent related with individual constitution and characteristics, its mechanism is multiple too, the pathology shows deficiency, heat, sputum and stasis.

Objective To study the relationship between lung inflammation and lung angiotensin Ⅱ ( ANG Ⅱ) in ventilator-induced lung injury ( VILI) and assessed the efficiency of the angiotensin-converting enzyme inhibitor captopril to attenuate VILI in rats. Methods Totally 21 adult male Sprague-Dawley rats were randomly assigned into 3 groups,high-volume,0 positive end-expiratory pressure ( HVZP) group; captopril injection ( 100 mg/kg i. p. ) in 30 min before HVZP ventilation ( HVZP + CAP group) ; no ventilation group ( control) . The blood gas tensions and mean arterial pressure were measured after a polyethylene catheter was placed in one carotid artery and a plastic cannula was inserted into the trachea. The protein contents and contents of macrophage inflammatory protein-2 ( MIP-2) in bronchoalveolar lavage fluid ( BALF) and lung ANGⅡ were determined by ELISA. The changes of lung pathology were observed by HE staining. Results Mean arterial pressure was significantly lower in the HVZP + CAP group than in the HVZP group after 2 hour’s ventilation. Total protein levels were significantly higher in BALF recovered from HVZP-ventilated rats than from controls. BALF MIP-2 and lung ANG Ⅱ were significantly higher in the HVZP group than in the control and HVZP + CAP groups. Lung ANG Ⅱ level was correlated positively with BALF contents of total protein and MIP-2. Conclusion Captopril has the efficiency to attenuate VILI by reducing inflammatory cytokines. Our results suggest that VILI is partly mediated by the local angiotensin system.

Objective To reveal the transmiting discipline of parameters and define time window for therapy of ischemia penumbra by continuous detection of diffusion tensor imaging(DTI), and evaluate the relations between cortical spinal tracts(CST) and myodynamia by diffusion tensor tract(DTT). Methods 71 patients of cerebral infarction with different timing were imaged with conventional MRI, DWI and DTI, measuring DCavg, FA, RA , 1-VR of different ROI and performing DTT in ten patients. Results DCavg of lesions in different phases were [(0.275?0.022),(0.349?0.019),(0.465?0.023),(0.538?0.035)]?10~ -3mm~2/s, respectively. DCavg value showed tendency of reducing obviously, elevating gradually and false-normalizing (P