Objective To observe the effect of enzymes-hydrolyzed wheat bran(EHWB) on antioxidant system in diabetic rats.Methods Diabetes mellitus models were induced by alloxan injection and EHWB was used to test its effect on anti-oxidative capacity;the EHWB was given intragastrically(i.g.),sodium ferulate(SF) as a positive group.The level of blood glucose,total anti-oxidative capacity(T-AOC),the activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and xanthine oxidase(XOD),and the content of malondiadehyde(MDA) were detected respectively in serum and liver of rats.Results EHWB significantly decreased the level of blood glucose and increased the level of T-AOC,as well as the activities of GSH-Px and SOD,and decreased the activity of XOD and the content of MDA.However,there was no difference between the groups feeding SF and EHWB.Conclusion EHWB can be used to decrease blood glucose and improve antioxidant ability in diabetes mellitus rats,the effect of EHWB is similar to SF.The effect of decreasing blood glucose of EHWB is related to protecting liver.

Objective To prospectively assess the association between cardiac resynchronization therapy (CRT )and left atrial volume .Methods Fourteen patients with chronic heart failure ,sinus rhythm and left bundle branch block were enrolled in this study and prepared for CRT implantation .Clinical and echocardiographic evaluations were performed before CRT implantation and 6 months later .Followings were parameters of the left atrial and left ventricular volume:left ventricular end-systolic volume index (LVESVi)and end-diastolic volume index(LVEDVi) ,maximal LAV index(iLAVmax) ,etc ,LAV index before atrial systole(iL-AVpre)and minimal LAV(iLAVmin) .CRT responders were defined as those whose reduction rates were more than 10% in iL-VESV in 6-month follow-up .Results Responders(71 .4% )and non-responders(28 .6% )had similar baseline ,clinical characteristics and pre-implantation LV volumes .However ,iLAVmax in the responders was remarkably lower than that in non-responders .CRT therapy significantly reduced LVESVi(P<0 .01) ,LVEDVi(P<0 .001) ,iLAVmax(P=0 .004) ,iLAVpre(P=0 .003)and iLAVpost (P<0 .01) ,but when compared with the non-responders ,iLAVmax was much lower in responders .Correlation analysis showed that there was a significant correlation of LVEDVi with iLAVmax ,iLAVpost(P<0 .05) .Conclusion CRT therapy can significant-ly improve iLAVpre and iLAVmax may be a predictor of LV reverse remodeling .

Objective To investigate the expression and activation of MMP-2, MMP-9 in cerebral tissues of cerebral ischemia-reperfusion rats and the effects of doxycycline on the cerebral edema. Methods Cerebral ischemia-reperfusion model was established by middle cerebral artery occlusion (MCAO). The rats were randomly divided into a sham-operated and 6 reperfusion groups according to different reperfusion periods (3 h, 6 h, 12 h, 24 h, 72 h, and 120 h groups). Dry weight method was used to evaluate hemispheric water content of the ischemic side after treatment with doxycycline. Western blotting analysis and Gelatin zymography were used to determine the expression and activity of MMP-2 and MMP-9 in the ischemic cerebral tissues. Results The protein expression and activity of MMP-2 were significantly increased in the ischemia tissue at 3 h and 120 h after reperfusion(P<0.01) ; the protein expression and activity of MMP-9 began to increase at 6 h after reperfusion (P<0. 01) , reached the peak at 24 h, and then returned to the basal levels at 120 h after reperfusion (P>0.05). The hemispheric water content of the ischemic side gradually increased with reperfusion periods compared with the sham-operated group. Dexycycline significantly reduced the hemispheric water contents compared with the normal saline at the same time points(P<0.05 or P<0.01). Conclusion MMP-2, MMP-9 can induce vasogenic brain edema by degrading basal membrane of capillary vessel. The alteration of MMP-2, MMP-9 expression and activities are likely associated with brain edema during cerebral ischemia-reperfusion.

Bronchial Diseases ; diagnosis ; surgery ; Bronchoscopy ; Child ; Humans ; Tuberculosis ; diagnosis ; surgery

Child ; Female ; Humans ; Job Syndrome

Objective To determine the clinical features of Klebsiella pneumoniae pneumonia and antimicrobial resistance of Klebsiella pneumoniae, providing the basis for rational use of antibiotics. Methods Eighty-six strains of Klebsiella pneumoniae were isolated from the sputum specimen which collected from the inpatients in our hospital from Dec 2008 to Dec 2010,which were identified with Bio Merieum Vitek 2. A total of 86 children were enrolled in the study, which were divided into hospital infection/colonization group (n = 68) and community infection group (n = 18). Clinical information were recorded. Extended spectum β-lactamase (ESBLs) and antibiotic susceptibility of Klebsiella pneumoniae were determined. Results Klebsiella pneumoniae pneumonia in children mainly occured in infants, especially in infants with basic diseases.Clinical manifestations of Klebsiella pneumoniae pneumonia had no much difference from common pneumonia. The detection rate of ESBLs was high(76. 7%). ESBLs were found in 64 strains of the hospital infection/colonization group (94. 1% ,64/68) ,while in 2 strains of the community infection group (11. 1% ,2/18).There was a significant difference between the two groups (P ＜0. 01). The drug resistance of hospital infection/colonization group was significantly higher than that of community infection group. The strains of hospital infection/colonization group were sensitive with cefotan, piperacillin/tazobactam, carberpenem, amikacin and ciprofioxacin only, while were highly resistant to penicillins, cephalosporins and monoamine antibiotics.The average hospital stay of the hospital infection/colonization group was (15.0 ± 7. 1) d, while the other was (8. 2 ± 3. 8) d. There was a very significant difference between the two groups in average length of stay (P＜0.01). Conclusion Klebsieila pneumoniae pneumonia in children mainly occurs in infants with basic diseases. The symptoms are untypical. Klebsiella pneurmoniae multi-drug resistance is a serious problem,which must be paid attention to. We should make a difference between the two groups when antibiotics were prescribed.

Bronchial Neoplasms ; diagnostic imaging ; etiology ; Child ; Hemangioendothelioma, Epithelioid ; diagnostic imaging ; physiopathology ; Humans ; Male ; Radionuclide Imaging ; Tomography, X-Ray Computed ; methods

Objective To investigate the association of the gene polymorphism of endothelial nitric-oxide synthase(eNOS) with pregnancy induced hypertension(PIH). Methods Sixty-six gravidas with PIH and 91 normal pregnant women(control) in the third trimester were investigated. The G894T mutation at exon 7 of the endothelial nitric-oxide synthase gene was studied by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results The frequencies of eNOS GG, GT and TT genotypes were 62.1%, 34.8% and 3.1% in PIH group and (79.1%,) 20.9% and 0.0% in control group, respectively (P0.05). Conclusions The variant (G-T) of 894 polymorphism site of eNOS is associated with the genesis of PIH, but not related to the severity of PIH. T allele may be a susceptible gene to PIH.

Objective: To evaluate the toxicokinetics and in vivo accumulation of norcantharidin lipid microsphere for injection (NCTD-LM) in Beagle's dogs following 90 d repeated iv administration by comparing with sodium demethylcantharidate injection (NCTD-I). Methods: An ultra high performance liquid chromatography-tandem mass spectrum method was employed to determine the NCTD in plasma of Beagle's dog after 1, 44, and 90 d repeated iv administration. The toxicokinetic parameters were calculated from the plasma concentration. Results: After the first iv administration of 0.8, 1.6, and 3.2 mg/kg NCTD-LM to Beagle's dog, the AUC0-t were (2.22 ± 0.53), (4.77 ± 1.13), and (13.43 ± 3.64) h∙mg/L, respectively, while the t1/2 were (1.37 ± 0.18), (1.64 ± 0.42), and (1.98 ± 0.25) h, respectively. After the 90 d repeated iv administration of 0.8, 1.6 and 3.2 mg/kg NCTD-LM to Beagle's dog, the AUC0-t were (3.58 ± 0.95), (11.4 ± 2.0), and (23.5 ± 3.9) h·mg/L, respectively, while the t1/2 were (3.87 ± 1.90), (5.75 ± 3.29), and (5.84 ± 2.45) h, respectively. For NCTD-I, the AUC0-t were (9.07 ± 2.09) and (14.1 ± 3.0) h∙mg/L respectively after 1 and 90 d repeated iv administration, while the t1/2 were (2.84 ± 1.34) and (3.53 ± 1.26) h. 15 and 30 d after the end of administration, the concentration of NCTD in plasma of Beagle's dog in each group were all dropped below the lowest limit of quantification (LLOQ). Conclusion: NCTD displays a nonlinear elimination at the dose range of 0.8-3.2 mg/kg after iv administration of NCTD-LM to Beagle's dog. Over the 90 d repeated iv administration period, the plasma concentration levels, AUC0-t, and t1/2 for both NCTD-LM and NCTD-I are increased with time increasing. However, no accumulation of NCTD is observed for the both formulations.

Objective • To investigate the distribution of geriatric syndromes in older adults in order to provide reference for the care of different types of the elderly. Methods • This study described distribution of 13 geriatric syndromes of 590 older adults from 5 hospitals and 6 nursing homes in Shanghai based on k-means clustering. The data were collected by Chinese version of continuity assessment record and evaluation (CARE) between May and November 2017. Results • 97.8% of the 590 older adults suffered from at least 1 geriatric syndrome. The top 5 geriatric syndromes were impairment in activities of daily living (80.7%), cognitive impairment (80.5%), polypharmacy (44.7%), visual impairment (45.3%), and hearing impairment (42.9%). The results of k-means clustering showed that the geriatric syndrome was divided into 4 clusters. Cluster 1 (named high incidence group of geriatric syndromes) exhibited high prevalence of impairment in activities of daily living, cognitive impairment, visual impairment, hearing impairment and delirium. On average, each older adult had 5.6±1.1 geriatric syndromes. Cluster 2 (named extremely high group) exhibited the highest prevalence of geriatric syndromes, excluding pain, depression and pressure ulcer. On average, each older adult had 8.3±1.1 geriatric syndromes. Cluster 3 was named low incidence group of geriatric syndromes, each older adult having 2.0±1.2 geriatric syndromes. The incidence of polypharmacy and fall was relatively high. Cluster 4 (named medium group) exhibited high prevalence of impairment in activities of daily living, cognitive impairment, polypharmacy, urinary incontinence and fall. On average, each older adult had 4.0±1.3 geriatric syndromes. Conclusion • Classification of geriatric syndromes is helpful to identify the high-risk older adults who may suffer certain patterns of geriatric syndromes, and thus to carry out targeted interventions.