Aim To identify the GABA transporter subtypes and to find its new associated member at the blood brain barrier.Methods Labeled by in vitro infusion of magnetic beads through carotid arteries,the brain microvessels without intact neural cells were sorted in magnetic fields,and identified by RT PCR.The homologous primer of GAT superfamily and the tRNA from isolated brain microvessels were used in the RT PCR to amplify target DNA.The PCR products were isolated by polyacrylamide gel electrophoresis(PAGE) and cloned, sequenced rospectively.The sequences were screened in dbEST of Genbank by Blast.Results Seven DNA bands were isolated from RT PCR products of isolated brain microvessels by PAGE. B3,B5 complete sequences were highly homologous with rat partial GAT 2 and BGT 1 respectively,B7 complete sequence was highly homologous with rat partial TAUT. The other 4 EST of B1(Accession No:CF358965), B2(CD568346), B4(CF358966) and B6(CD568347) were submitted to dbEST,they were homologous with some sequences in Genbank,but were not homologous with GAT members.Conclusion GAT 2 and BGT 1 of GAT and TAUT were localized at the blood brain barrier which might be responsible for the GABA transport across the blood brain barrier.The genes and their functions of 4 EST associated with GAT need to be clarified.

Objective To explore the relationship between Monoamine oxidase A (MAOA) receptor gene,catechol-O-methyltransferase (COMT) receptor gene,dopamine D3 receptor(DRD3) gene and 5-HT2C receptor gene(5-HT2c) of Han population in northern China and obsessive compulsive disorder.Methods Polymerase chain reaction amplification determination of MAOA-T1460C,COMT-Val158Met,DRD3-Ser9Gly,5-HT2c-C ys23Ser four loci receptor gene polymorphism in 164 patients with OCD patients including 103 core pedigrees of fragment length polymorphism,and association and linkage disequilibrium (TDT)analysis.Results There was no significant difference of MAOA-T1460C,COMT-Val158Met,DRD3-Ser9Gly,5-HT2c-Cys23Ser four receptor gene in the patient group and the control group of genotype and allele distribution difference(P＞0.05),four receptor gene loci were in accordance with the balance of the H-W,the MAOA-T1460C receptor gene in female patients group and control group,the early group and control group,which has forced thinking and difference of compulsive behavior group and the control group,only the obsessional group and the control group of genotype and allele distribution was statistically significant(P＜0.05),and the family group between chain(P=0.0001) ;5-HT2c-Cys23Ser receptor gene in the case group and the control group,male both forced thinking and compulsive behavior group and control group differences in genotype and allele distribution was statistically significant (P＜ 0.05),and between family groups exist chain (P=0.0389) ; COMT-Val158Met receptor gene in the control experiments were no significant difference(P＞0.05),and with the house group does not exist between the chain (P=0.0622) ;DRD3-Ser9Gly receptor gene in the control experiments were no significant difference(P＞0.05),and with the family groups there is no chain(P=0.1101).Conclusion MAOA-T1460C receptor gene polymorphism and 5-HT2c-Cys23Ser receptor gene polymorphisms may be the susceptible gene of obsessive compulsive disorder.

New progress of myelodysplastic syndomes (MDS) in the 55th ASH annual meeting on 5-azacytidine and decitabine was reviewed.Except in older females,the survival time for a diagnosis of MDS in the United States does not seem to have substantially improved since the introduction of 5-azacytidine into the market in 2004.However,clinical and mechanism data suggest that judicious,mechanism-based application and optimization of these agents will have increasingly meaningful impact.Moreover,these drugs are exemplars of an inspiring alternative to the traditional cytotoxic paradigm and should pave the way for additional relatively nontoxic but broadly effective agents.

Objective To study the changes of apoptosis in brain after traumatic injury(TBI)treated with exogenous vascular endothelial growth factor(VEGF)gene therapy in oder to find out the role of exogenous VEGF gene in protectiog brain tissue.Method The injurea cerebral cortex from the rat models of brain with traumatic injury was injected with adenovirus(adenovirus,Ad)as the carrier of VEGF-165 gene(Ad-VEGF-165 Gene).RT-PCR and Western blot were used to detect the expression of VEGF mRNA and VEGF protein in the brain 6 h,24 h,3 d,7 d and 14 d after injury,and apoptosis in the injured location of brain was also detected by TUNEL successively after Ad-VECF-165 gene therapy.Results With exogenous Ad-VEGF-165 applied to the locally injured brain tissue after injury,VEGF mRNA and VEGF protein showed consistent expression and their expressions were significantly higher than those in trauma group and vehicle control group.Compared with the trauma group.the apoptosis in the gene therapy group 24 h,3 d and 7 d after injury presented a.significant reduction,and had close relationship with VEGF.(Control group:4.17±0.73;TBI group,24 h:47.18±6.01,3 d:79.44±11.23;TBI+VEGF group.24 h:28.72±5.31,3 d:54.18±7.66;P＜0.05).Conclusions The exogenous VEGF gene therapy administered to have protective effects on the local brain tissue in rats with traumatic injury in a certain time.

Objective To study the follow-up value of MRI in the sequela hypoxic-ischemic encephalopathy(HIE).Methods MRI features of HIE in 81 neonates were analyzed retrospectively,every child had two cranial MRI scans,the first scan at the end of the newborn stage,the second scan at 4 months to 4 years.There were 66 full-term neonates and 15 preterm neonates.Results Of 81 cases,79 cases were abnormal on second MRI examination,including myelinogenetic abnormal in 59 cases(72.8%),external hydrocephalus(EH) in 53 cases(65.4%),brain malacia in 26 cases(32.1%),cerebaral atrophy in 25 cases(30.1%),white matter decrease in 24 cases(29.6%) and dysplasia of corpus callosum in 9 cases(11.1%).EH(26/35cases) and myelinogenetic abnormal(16/35 cases) in mild HIE group,brain malacia(12/29 cases) in moderate HIE group,cerebaral atrophy(16/17 cases) and white matter decrease(15 /17 cases)in severe HIE group were often seen.Conclusion MRI findings can reflect the pathological changes of sequela of HIE,and provide objective basis for diagnosis and assessment of prognosis.

The indoor air formaldehyde pollution has some adverse effects on human health. A number of researches have been reported on appropriate fitment and other measures to reduce the harm of indoor air formaldehyde pollution. The research progress of pollution control measures and the adverse effect of formaldehyde on human health were reviewed in the present paper.

Objective To study the compound oral contraceptives(COC)application in patients with missed abortion after.Methods 600 cases of missed abortion from March 2016～2017 year in March in our hospital,were randomly divided into observation group(n=300)and control group(n=300),the observation group was treated with compound oral contraceptive therapy,the control group were given routine treatment,compared two groups of patients.Results The observation group of patients with vaginal bleeding time,menstrual recovery time was significantly shorter than the control group(P<0.05),endometrial thickness was significantly greater than the control group(P<0.05),the incidence of Intrauterine Residue and uterine cavity adhesion rate,pelvic infection rate was significantly lower than the control group(P<0.05); the two groups of patients with postoperative weight change in March the amount of vaginal bleeding,compared no significant difference,no statistical significance.Conclusion The application of compound oral contraceptives in the treatment of missed abortion patients after curettage,shorten the time of vaginal bleeding,reduce the amount of vaginal bleeding,reduce the incidence of postoperative complications and promote postoperative recovery of menses.

OBJECTIVE:To study the safety of polylatic acid used as sustained-release drug carrier in brain.METHODS:The toxicity and apoptosis of in vitro primary cultured cortical neurons in rats were evaluated using CCK-8 and Hoechst33258 staining after treated with polylatic acid and compared with the normal control group.Self-made polylatic acid blank tablet was implanted into rat brain and after 30 days the neuronal function was assessed through behavior ability testing,HE staining and Nissl staining,compared with sham groups and normal control group.RESULTS:When neurons were cultured for 7,9 and 12 days in polylatic acid group,there was no significant difference in absorbance value of CCK-8 and apoptosis rate between normal control group and polylatic acid group.Light inflammatory was observed in rat brain tissue in polylatic acid blank tablet group after 30 days,as compared with sham group.But the morphology,number and function of neuron had no obvious change.CONCLUSION:Polylactic acid has no significant inhibitory and inducing apoptosis effect on in vitro cultured cortical neurons and also has no obvious toxicity on brain tissue.

Twenty four hours after occluding of the middle cerebral artery in rats, thecirculating neutrophils were in activated state and the lipidperoxidation increased in ische-mic brain tissue. Tanshinone inhibited the chemoluminescence of the circulating neutrophilphagocytosis and decreased leukocyte infiltration in infarcted brain tissue. It alsodecreased lipoperoxide, malondialdehyde and increased SOD activity in ischemic cerebralregion. In addition, it reduced cerebral infarction size in the model. The correlationbetween inhibitory effects on leukocyte and reduction in ischemic cerebral damage wassignificant. Dexamethasone used as a positive control had similar effects on neutrophils andischemic cerebral injury. It was shown that the prophylactic effcts of Tanshinone oncerebral infarction might be mainly due to its inhibition on leukocyte function.

Objective To investigate the changes of region cerebral homodynamic and cerebral vascular autoregulation in traumatic brain injury(TBI) by dynamic CT perfusion(CTP) imaging in rat.Methods Forty-five SD rats underwent CTP,including 10 control rats and 35 TBI rats,on 1,6,24 h,and day 3,7,14,21 after TBI.The region of interest(ROI) was located near traumatic area within 2 mm and mirror area of relative brain hemisphere.False color pictures and dynamic rCBF,rCBV,MTT and PS were obtained in different time.Pathological examination was also done.Results The rCBF,rCBV on 1 h after TBI was lower than that of control group,and significantly higher on 6-24 h after TBI.They reached the peak on day 3.PS had a similar change compared with rCBF,rCBV,but a longer duration.MTT showed to moderate changes except an isolated tide on 3 days after TBI.The rCBF on mirror area of relative brain hemisphere was almost stable except 3 days after TBI,which was lower than that of control group.Meanwhile,the rCBV on mirror area of relative brain hemisphere was normal.Conclusion CTP is an advanced technique to investigate the changes of region cerebral homodynamic and cerebral vascular autoregulation in TBI.The early hypoperfusion and next hyperperfusion around traumatic areas after TBI suggest that the dysfunction of cerebral vascular autoregulation should be committed to the change of homodynamic in TBI.