Objective To study the correlation between diabetes and the new discovered angiopietin-related growth factor (AGF)that participates in energy metabolism.MethodsA total of 24 male C.57BL/6 mice were divided into three groups:controlled group; fed with high-fat diet( HFD); fed with high-fat diet for 10 weeks and then treated with rosiglitazone ( HFD + RSG) ; The relative level of AGF mRNA expression in livers was measured by determining a ratio of PCR products of AGF to that of β-actin gene.Oral glucose tolerance test,and insulin resistance index (HOMA-IR) were used to measure blood glucose level and insulin sensitivity.Results Compared with the controls,the expression of AGF mRNA in liver decreased significantly in HFD group( P ＜0.05),and increased significantly in HFD + RSG group( P＜0.01 ).A negative correlation was found between AGF mRNA expression in mice livers and HOMA score ( r =-0.516,P ＜ 0.05 ).Conclusions AGF as a new hepatocyte derived circulating factor counteracts obesity and is related to insulin resistance.

Objective of electroacupuncture(EA) of Alzheimer′s disease(AD) rat learning and memory ability and plasma SOD , MDA content in brain P53 ,P21 expression .Methods The normal 36 SD rats ,were randomly divided into sham operation group , model group and EA group .EA group acupuncture “Baihui” ,“Taixi” ,“Zusanli” treatment ,through the Morris water maze test in rats behavioral changes ,spectrophotometric observation of SOD and MDA in rat plasma levels ;observation of rat brain P53 ,P21 ex-pression by immunofluorescence .Results Compared With the model group ,group EA escape latency ,longer in the third quadrant of the activities of the time ,the number of crossing platform position increased ,decreased the elevated plasma SOD level ,MDA lev-el ,reduce the expression of brain P53 ,P21 ,and the difference was statistically significant (P<0 .01) .Conclusion electro-acupunc-ture treatment can improve the Aβ1-42-induced AD rat spatial learning and memory ability ,may be lowered P53 ,P21 protein ex-pression increase SOD activity and MDA .

Objective To study the effects of miR-130b on the neuronal migration in the developing embryonic mice cortex.Methods Pregnancy E15.5 mice were selected and plasmid of miR-130b was injected into the lateral ventricle of the embryonic brain.Two days after eletroporation,murine embryos were collected and cut into frozon coronal slices,then surveyed the neuronal migration under the fluorescence microscope.Results The neuronal migration rates were higher in miR-130b overexpression embryonic cortex,of which 75.1％ eletroporated neurons migrated into MZ,compared with the 22.1％ in control group.On the contrary,only 7.9％ eletroporated neurons stayed in the VZ/ SVZ in the experimental group,compared with the 69.3％ in control group.Conclusions miR-130b accelerates the neuronal migration in the developing embryonic mice cortex,its regulational role is worth studying.

ObjectiveTo investigate the clinical and imaging features of nonaneurysmal perimesencephalic subarachnoid hemorrhage (NAPSAH).MethodsThe patients with NAPSAH were retrospectively enrolled, and their clinical and imaging features were collected and analyzed.Results In 489 patients with subarachnoid hemorrhage treated over the same period, a total of 23 (4.7%) with NAPSAH were diagnosed with cranial CT and CT angiography.Subarachnoid blood centered in the prepontine cistern in 4 patients (17.39%), interpeduncular cistern in 8 (34.78%), ring pool in 7 (30.43%), and quadrigeminal cistern in 4 (17.39%).Two patients were transferred to other hospital for treatment, and the remaining 21 patients had a good outcome without complications and neurological deficits.Among them, the modified Rankin scale scores in 17 patient were 0, and 4 were 1.Their cardinal symptoms were forgetfulness, irritability, anxiety, and other subjective symptoms.They were improved after receiving psychological counseling and anti-anxiety treatment.ConclusionsThe incidence of NAPSAH is low.The clinical symptoms in patients with NAPSAH are mild, the outcomes are better without obvious complications.

Objective To observe the influence of cervical traction and manipulation on hemorheology of cervical vertigo patients.Methods 59 patients in outpatients of our hospital from January 2012 to August 2013 were recruited and divided into a control group(29 patients)and a treatment group(30 patients)randomly. The control group was treated with flunarizine, and the treatment group was additionally treated with cervical traction and manipulation on the basis of the control group. After 4 therapeutic causes, evaluation of cervical vertigo symptoms and functions(ESOV)were performed and hemorheological changes were observed in both groups.Results① ESOV comparison: ESOV sores in both groupswere obviously improved after the treatment than the same group before the treatment(t=11.028, 5.825,all P<0.01)the treatment group were significantly lower than that in the control group after treatment(t=3.6057,P<0.01).② hemorheology changes comparison: whole blood viscosity(high-, medium- , low shear), whole blood reduction viscosity, erythrocyte aggregation index, hematocrit in the treatment group were improved obviously after the treatment than before(t=3.356, 13.245, 3.650, 7.443, 9.912, 3.113,P<0.05 orP<0.01). The whole blood viscosity(high-, medium-shea), blood reduction viscosity, erythrocyte aggregation index in the control group were obviously improved than the before(t=2.144, 4.216, 3.287, 3.039, 6.442,P<0.05 orP<0.01). The difference between the two groups were also obvious after the treatment(P<0.05 orP<0.01).Conclusions Cervical traction and manipulation combined with flunarizine can elevate ESOV score, improve hemorheology and therapeutic effect in patients with cervical vertigo.

This paper focuses on the requirements of joint operations under informationization in the future,and system-atically analyzes the characteristics of medical protection with regard to epidemic causes and surveillance,prevention meas-ures,mental health,and nuclear,chemical and biological protection.This paper elaborates on the basic principles of medical protection and raise proposals about health and epidemic protection so as to provide references for health support of PLA during joint operations.

HL-60 cells treated with 80?g/ml notogensenosidc R_1, were induced to granulocytic differentiation to 68% (among which metamyelocytes 32%, banded neutrophils 30% & segmented neutrophils 6%). Further experiment showed that the ability of the cell to reduce nitroblue tetrazolium (NBT) dye, phagocytosis, as complement receptors and activities of acid phosphatase and ?-glucuronidase were all enhanced after differentiation. There were significant differences between the treated groupand the control group in NBT positive rate (62% v. s. 10%, P

Objective To explore the difference between the results of the cross-disciplinary joint teaching method and the traditional teaching method in medical humanity courses.Method 128 students in one military medical university were selected to be randomly assigned to the study group and the control group,using the cross-disciplinary joint teaching mode (study group,n=64) and traditional teaching mode (control group,n=64) in medical humanity courses.For the joint teaching mode,we compiled the teaching materials of medical humanities,formed the teaching team and organized the academic communication after class by social media.After all courses were finished,the subject cognition and approval of whole students were evaluated by the unified arranged examination and questionnaire.Then the data was processed by SPSS 18.0.Results On the aspect of subject cognition,the total scores of the study group were higher than control group in health law [(76.2 ± 3.34) vs.(72.5 ± 4.76);t=3.32,P=0.000] and medical ethics [(75.3 ±2.93) vs.(68.3 ± 3.10);t=4.01,P=0.000].On the aspect of subject approval,the recognition of the students of the study group in these three subjects were more than those of the control group except the selfevaluation of medical psychology (P=0.000).Conclusion The application of cross-disciplinary joint teaching method on medical humanity courses contributes evidently to the promotion of the medical humanity quality of clinical medical students.To implement this model,however,multi-sided resources are needed for colleges to coor-dinate,for perfect results require more practical exploration.

Frailty is a pre-disabled stage and associated with increased vulnerability to adverse health outcomes. Frailty has high preva-lence in older population. There are 2 principal emerging models of frailty, which are the phenotype model and the frailty index model. Many studies have developed tools to detect frailty, including self-report scales, such as PRISMA-7, Groningen Frailty Indicator (GFI), Ed-monton Frail Scale (EFS) and Simple Frailty Questionnaire (FRAIL);and single assessments, such as gait speed, timed up-and-go test, grip strength, pulmonary function test. Intervention of exercise and/or nutrition are benefical for frail older adults.

Objective: To study the effect of volatile oils from Foeniculum Vulgare Mill, anisaldehyde, anethole and water extracts on the percutaneous penetration of 5 fluorouracil. Methods: With Valia Chien horizontal diffusion cell and HPLC method to observe the effect of volatile oils of Foeniculum Vulgare Mill, anisaldehyde, anethole on the percutaneous penetration of 5 fluorouracil. Results: The volatile oils of Foeniculum Vulgare Mill, anethole, anisaldehyde possessed the high promoting action on percutaneous absorption of 5 fluorouracil, and their enhancement ratio are 7.14, 4.17, 9.54, respectively. Conclusion: The volatile oils of Foeniculum Vulgare Mill, anethole, anisaldehyde enhance the skin permeation of 5 fluorouracil effectively.