The present paper reports a determination of copper, zinc and aluminium in caudate nuclei in 8 adult cadavers and in 15 newborn cadavers by atomic absorption spectrophotometry using the graphite furnace atomizer. The results showed that the normal content of copper in the adult caudate nucleus was 24.04?8.93mg/kg dry tissue, zinc 129.92+89.17mg/kg and aluminium 2.38?0.77mg/kg. In the newborn cadavers, the normal contents of copper zinc and aluminium were 11.00+5.49, 75.35+27.52 and 1.72?0.56mg/kg dry tissue respectively. These results indicate that the data Iisted above in adults are higher than in newborn in fants.

Object To survey the worldwide medicinal plant resources of Illicium L Methods On the basis of herbarium, we studied on more than 10 000 speciemens of Illicium L from 121 Herbaria in 18 countries and the reviewed literature Results Fifteen species in Illicium L in the world belong to the medicinal plants, and they distribute in China, India, Burma and Vietnam In addition, the province scale distribution, habitat preference, and medicinal value are provided for each of the 15 medicinal plants Conclusion The medicinal plant resources of Illicium L from China are the most abundant in the world, and should be rationally exploited and widely utilized

In order to study the light propagation in biological tissue, we analyzed the divergent beam propagation in turbid medium. We set up a Monte Carlo simulation model for simulating the divergent beam propagation in a semi-infinite bio-tissue. Using this model, we studied the absorbed photon density with different tissue parameters in the case of a divergent beam injecting the tissue. The simulation results showed that the rules of optical propagation in the tissue were found and further the results also suggested that the diagnosis and treatment of the light could refer to the rules of optical propagation.
Computer Simulation ; Light ; Models, Biological ; Monte Carlo Method ; Optics and Photonics ; Scattering, Radiation

Objective:Peripherally inserted central catheter(PICC) has been application in clinical for many years,but the training in the knowledge about maintenance wasn't univesal systematically yet.To analyze the existed problems on the clinical peripherally inserted central catheter in washpipe technology and find out countermeasures. Methods: The self-made questionnaire was used to survey a total of 46 nurses in every department and make on-site operation demonstration. Results: Only 12 nurses' operation was completely correct and the correct rate was 26.1percent,which was related to the training,manager's attention,nurses' participate awareness. Conclusion: The training on nurses' operation of red tube needed to be strengthen.

Lyme disease is continuing worldwide spread and its prevalence still presents raising tendency.The causative agent is Borrelia Burgdorferi(Bb) and the special biological intermedia is ixodid ticks(Ixodes scaputaris).The current study on the genetic strain of Bb and the interpretation of laboratory diagnosis(microscopic,immunologic and biomolecular test)results as well as the preparation of vaccine for the disease are made in this review.

Autophagy is a physiological process which delivers the mutant cytoplasmic proteins and dysfunctional subcellular organs into lysosomes for degradation to generate fuel in the deficiency conditions. It is mainly classified into macroautophagy, microautophagy and chaperon-mediated autophagy (CMA), as well as the selective autophagy such as mitophagy and aggrephagy. This review mainly introduces the key molecular markers of macroautophagy, CMA and mitophagy.
Autophagy ; Humans ; Lysosomes ; Mitochondrial Degradation ; Molecular Chaperones

BACKGROUND: Although clinical methods for autologous peripheral blood stem cell separation and identification arerelatively more, there is no unified standard. Moreover, little is reported on immune effects on lung adenocarcinomaduring autologous peripheral blood stem cell transplantation.OBJECTIVE: To study the effect of autologous peripheral blood stem cell transplantation on immunological function oflung adenocarcinoma rats by exploring the separation and identification methods of autologous peripheral blood stemcells.METHODS: The mononuclear cells were isolated from the peripheral blood of Wistar rats by density gradientcentrifugation method and then identified. Eighteen Wistar rats were randomly divided into routine treatment group andcell transplantation group, with nine rats in each group. Animal models of lung adenocarcinoma were established in therats, and underwent no treatment in the routine treatment group but injection of autologous peripheral blood stem cellsuspension (0.2 mL, 2.5×106/L) via the tail vein in the cell transplantation group. Immune indicators were detected in twogroups after treatment. Three rats from each group were anesthetized and scarified at 4, 10, 14 days after treatment,respectively. Afterwards, we detected the effects of autologous peripheral blood stem cell transplantation onimmunological function of lung adenocarcinoma rats.RESULTS AND CONCLUSION: Newly isolated mononuclear cells from rat peripheral blood showed a round or ovalshape and were relatively small in size. After 5 days of culture, autologous peripheral blood stem cells grew rapidly, andthe round cells began to spread to both ends and became spindle-shaped, thereby forming small colonies. Autologousperipheral blood stem cells induced in vitro experienced three stages successively: cell arrest, logarithmic and plateauphases. Flow cytometry showed that sorted cells were identified as autologous peripheral blood stem cells by expressingCD90, CD133 and Flk-1. After 4, 10 and 14 days culture, the expression of CD133 increased first and then descended,and Flk-1 showed an increasing trend. Compared with the routine treatment group, the expression of tumor necrosisfactor-α induced protein 6 in the cell transplantation group was significantly higher (P < 0.05), while the expression ofnuclear factor κB was significantly lower (P < 0.05). In conclusion, the isolation and identification methods of autologousperipheral blood stem cells are simple and can be used to secrete a large number of nutritional factors in the treatment oflung adenocarcinoma, which can enhance body immunity in the treatment.

Autophagy is a physiological process which delivers the mutant cytoplasmic proteins and dysfunctional subcellular organs into lysosomes for degradation to generate fuel in the deficiency conditions. It is mainly classified into macroautophagy, microautophagy and chaperon-mediated autophagy (CMA), as well as the selective autophagy such as mitophagy and aggrephagy. This review mainly introduces the key molecular markers of macroautophagy, CMA and mitophagy.

DNA methylation is one of the regulators on the expression of genes,and the expression of genes can be ”silenced” by hypermethylation of the tumor suppressor genes,which is in close relationship with carcinogenesis.Gastric cancer is caused by multiple factors and multiple genes alteration and prolonged interaction.among them ,There is considerable relation between the hypermethylation of the tumor suppressor genes and the gastric carcinogenesis.

As antigen-presenting cells, DCs are the most potent and unique,they can stimulate the primitive T cells. They play a major role in initiating the immune response. Tumor infiltrating dendritic cells (TIDCs) can reflect the antitumor immune capability of the host. The density of TIDC perhaps is associated with the prognosis in some cancers. The disablement of TIDCS is partly due to the phenotype they have. Perhaps it is the immunosuppressive factors secreted by tumor cells that have changed their phenotype.The studies about TIDCs provide the basis for DC-mediated antitumor immune therapy,and suggest the use of DC to treat tumors through different approaches.