Application of imatinib has made a lot of chronic myeloid leukemia (CML) patients longterm survival,but the CML BCR/ABL1 kinase domain mutations (KDM) will result in drug resistance of imatinib.There are many KDM types.The different types appear randomly.The leukemic clone with different KDM characteristics has a certain evolution.In the period of imatinib treatment,a higher degree of drug resistance mutations in cells likely to develop as the dominant clone.It is suggested that the treatment of imatinib-resistant CML in addition to efforts to develop next-generation tyrosine kinase inhibitors outside,but also consider the use of traditional medicine to help cure.

Polymerase chain reaction (PCR) technique for the diagnosis of hematological disease brought about revolutionary progress. It has become indispensable and basic technology for genotyping of acute and chronic leukemia and diagnosis of myeloproliferative disorders, thalassemia, hemophilia and other genetic diseases. Based on the PCR technology, multiple nested-PCR, real-time fluorescence quantitative PCR (Q-PCR), denaturing high pressure liquid chromatography (DHPLC), capillary electrophoresis, the molecular hybridization and directly sequencing technique have been developed. The multip]e nested-PCR can be used to examine a variety of leukemia genes and confirm the type of leukemia. The application of Q-PCR has effectively solved the problem of pollution of the PCR and it can be used to monitor minimal residual disease of leukemia. Capillary electrophoresis is more suitable for gene fragment length change. DHPLC can be applied for high-throughput screening of gene mutation. Sequencing is a reliable method and it has been used for routine diagnosis.

With the fierce competition of higher education market,the public university and college face the pressure from mana-gement toward economic operation.This article tries to make some study on what the economic operation innovation of the public higher education institutions is,to make a synopsis analysis on disadvantageous factors and advantageous factors,and to put forward some suggestions on its promising system.

Thirty-four patients with acne vulgaris were treated with pricking and ear-pressure. Acupoints were Baihui (GV 20), Dazhui (GV 14), Taodao (GV 13), Shenzhu (GV 12), Shendao (GV 11), Lingtai (GV 10), Feishu (BL 13), and Sanyinjiao (SP 6), for priking. Lung (MA-IC1), Stomach (MA-IC),Endocrine (MA-IC3), Subcortex (MA-AT1), EarShenmen (MA-TF1) and Cheek (MA-L) were applied for ear-pressure. After 4 courses' treatments, 33 cases obtained effectiveness and 1 case ineffectiveness.

Immunophenotyping with flow cytometry can clearly identify multiple myeloma cells and monitor minimal residual disease by using the characteristic of light chain restriction in plasma cells and some cell surface markers( eg CD138, CD38, CD56, CD117 ). This technology may provide important evidence in the diagnosis of multiple myeloma.

Objective To observe the efficacy of rosiglitazone treatment in impaired glucose tolerance (IGT) with hyperinsulinemia. Methods 56 IGT patients were divided into two groups randomly:control group (28 cases) and rosiglitazone group (28 cases). Improving life style were given to control group,while improving life style and rosiglitazone (4mg/d) were given to rosiglitazone group for 12 weeks. The body mass index (BMI),fasting and postprandial plasma glucose,insulin and insulin sensitivity index (ISI) were observed. Results In control group the fasting and postprandial plasma glucose and insulin showed no difference between pre-and post-treatment (P>0.05).The levels of the fasting and postprandial plasma glucose and insulin dropped obviously in rosiglitazone group compared to both the baseline levels and the levels of control group at 12 weeks of treatment(P<0.05). The BMI and body weight dropped obviously in control group at 12 weeks of treatment (all P<0.05),but showed no difference in rosiglitazone group (P>0.05). The ISI increased markedly in rosiglitazone group (P<0.05),but showed no difference in control group (P>0.05). Conclusions The rosiglitazone treatment increases insulin sensitivity obviously,improves first-phase insulin secretion,and postpones the progression to T2DM from impaired glucose tolerance in patients with IGT

To compare the degree of glycemic excursions by dynamic glucose monitoring in type 2 diabetes in the elderly when their HbA_1c≤6. 5, The mean blood glucose level and hypoglycemia incidence were observed. The amplitude of glycemic excursions revealed by DGMS was significantly lower in insulin glargine group than in mixed insulin group (P<0.05). The incidence of hypoglycemia were lower in glargine group than in mixed insulin group(P<0.05).

Cutaneous wound healing is a complicated multiatep process with numerous mediators that act in a network of activation and inhibition processes. Nonhealing chronic wounds decrease the quality of Life. Recombinant growth factors, currently used in clinic, fail to provide a sustained repair function at the wound due to their inadequate biological availability and transient half-time. Recent attention has focused on gene therapy, which might become a significant treatment modality for those wound healing pathologies refractory to other wound management approaches. This review discusses the potentials and limitations of current genetherapy for the treatment of wounds, current ongoing clinical trials, and possible future directions in this exciting field.

Objective To investigate the effect of protamine(PS)coated polylactic acid(PLA)nanocapsule on the immune response of murine bone marrow-derived dendritic cells(BMDCs).Methods PLA nanocapsules were prepared by W1/O/W2 method.Protamine was used to modify the surface of PLA nanocapsule that encapsulated ovalbumin(OVA),a model anti-gen.The prepared OVA/PLA/PS nanocapsules were used to stimulate BMDCs.Flow cytometry was used to analyze the ex-pression of surface molecules CD80,CD83,CD86,MHCⅠ,and MHC Ⅱ,and the uptake of nanocapsule.The levels of IL-12 p70 secreted by BMDCs were detected by ELISA.Results OVA/PLA/PS nanocapsules could significantly up-regulate the expres-sion of CD80,CD83,MHCⅠ,and MHC Ⅱ in BMDCs,and increase the secretion of IL-12 p70 by BMDCs.Furthermore,OVA/PLA/PS nanocapsules could enhance the uptake efficiency of OVA by BMDCs.Conclusion OVA/PLA/PS nanocapsule can en-hance the immune response of BMDCs,and may become a good drug delivery carrier.

Mantle cell lymphoma (MCL) is a rare subtype of B-cell non-Hodgkin's lymphomas (NHLs). MCL comprises distinct subtypes with different pathological characteristics and clinical features. However, MCL remains incurable by intensified first-line regimens con-taining cytarabine and involving consolidation with high-dose therapy and autologous stem cell transplantation. Recently discovered somatic mutations and aberrant intracellular signaling pathways have been demonstrated to play an essential role in the pathogenesis of MCL. The related novel therapeutics, such as Btk inhibitors, PI3K inhibitors, and immune modulators, have exhibited promising ther-apeutic effects on untreated or even relapsed/refractory MCL. The development of an efficient combination therapy is urgently need-ed to improve the survival of MCL patients in the future.