2.Research development of proteomics in pancreatic cancer.
Li NING ; Tai-ping ZHANG ; Yu-pei ZHAO
Acta Academiae Medicinae Sinicae 2005;27(5):640-643
This review covers the development of proteomics in pancreatic cancer, including the research strategy, technology, content, and problems.
Cell Line, Tumor
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Humans
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Mass Spectrometry
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methods
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Pancreatic Neoplasms
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chemistry
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diagnosis
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physiopathology
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Protein Array Analysis
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methods
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Proteomics
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methods
3.Effect of botulinum toxin type A on the expression of substance P, calcitonin gene-related peptide, transforming growth factor beta-1 and alpha smooth muscle actin A in wound healing in rats.
Lin WANG ; Ning-zheng TAI ; Zhi-hong FAN
Chinese Journal of Plastic Surgery 2009;25(1):50-53
OBJECTIVETo investigate the effect of botulinum toxin type A on the expression of substance P (SP), calcitonin gene-related peptide (CGRP), transforming growth factor beta-1 (TGF-beta1) and alpha smooth muscle actin A (alpha-SMA) in wound healing.
METHODS60 rats were randomly divided into group C (control) group L (low-dose) and group H (high-dose), with 20 rats in each group. The wound-healing model was established by excision of four full-thickness skin (1 cm x 1 cm, around the injection site) on the back of all SD rats on the 7th day after BTA injection. The wound size was measured and the expression of SP, CGRP, TGF-beta1 and alpha-SMA in wound granulation tissue was assayed by immunohistochemical staining and computerized image analysis before operation, and 3 days, 7 days and 14 days after operation.
RESULTSAll the wounds healed 14 days after operation. The wound size in L and H group was not significantly different with that in C group on the 3rd day and 7th day after operation. The positive immuno-staining of SP, CGRP, TGF-beta1 and alpha-SMA in group L and H was significantly weaker than those in C group. Meanwhile, the positive immuno-staining of all above substances in H group was weaker than those in L group significantly.
CONCLUSIONSBotulinum toxin type A can decrease the expression of SP, CGRP, TGF-beta1, and alpha-SMA in wound healing in a dose-dependent manner with no effect on the healing time.
Actins ; metabolism ; Animals ; Botulinum Toxins, Type A ; pharmacology ; Calcitonin Gene-Related Peptide ; metabolism ; Male ; Rats ; Rats, Sprague-Dawley ; Skin ; drug effects ; metabolism ; Substance P ; metabolism ; Transforming Growth Factor beta1 ; metabolism ; Wound Healing ; drug effects
4.Relationship between Leu72Met polymorphism of Preproghrelin gene and type 2 diabetes mellitus and diabetic nephropathy
jia-mei, JIANG ; yong-ning, SUN ; li-mei, LIU ; tai-shan, ZHENG ; nian-song, WANG ; feng, WANG
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(07):-
0.05). Conclusion Preproghrelin-Leu72Met is not significantly associated with T2DM and DN in Shanghai Han populations,while T2DM with AA genotype is characterized by significant declination in urine microalbumin when compared with CA and CC genotypes.Leu72Met polymorphism(C→A)may postpone the development of microalbuminuria in T2DM subjects.
5.Proteomic analysis of membrane proteins of endomembrane system in human pancreatic cancer cell.
Bo PAN ; Yu-pei ZHAO ; Li NING ; Tai-ping ZHANG ; Quan LIAO
Chinese Journal of Surgery 2010;48(6):461-465
OBJECTIVETo analyze and identify the membrane proteins of endomembrane system in human pancreatic cancer cell by proteomics.
METHODSMembrane protein was extracted from pancreatic cancer cell lines Capan-1, MiaPaCa-2, Panc-1. The membrane protein mixture of the three pancreatic cancer cell lines were separated by two-dimensional electrophoresis (2-DE). Positive dots of staining 2-DE gel were identified by MALDI-TOF mass spectrometry and PMF matching, and then evaluated by bio-informatics searching in NCBI and ExPASy databases. Information of membrane proteins were acquired like sequence, molecular weight, isoelectric point, location and biological functions.
RESULTSForty-nine membrane proteins out of 166 protein dots which could be seen on the 2-DE gel were identified as channel carrier proteins (4 proteins), signal transduction proteins (5 proteins), transcription regulatory and translation modification protein (7 proteins), proliferation and apoptosis related proteins (4 proteins), invasion and migration associated proteins (2 proteins), cytoskeleton proteins (3 proteins), metabolism pathway proteins (14 proteins), and function unknown protein (10 proteins).
CONCLUSIONSEndomembrane proteins of pancreatic cancer cell play key roles in tumor malignant behavior like proliferation, metabolism, motility, adhesion and migration. These membrane proteins might become candidate biomarkers or targets of therapy of pancreatic cancer.
Biomarkers, Tumor ; analysis ; Cell Line, Tumor ; Electrophoresis, Gel, Two-Dimensional ; Humans ; Membrane Proteins ; analysis ; Pancreatic Neoplasms ; metabolism ; Peptide Mapping ; Proteomics ; methods ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
6.Screening for peptides of anti-rotavirus by phage-displayed technique.
Ning YAO ; Lun-Guang YAO ; Xiang-Man ZHANG ; Tai-Lin GUO ; Yun-Chao KAN
Chinese Journal of Biotechnology 2007;23(3):403-408
In this study, a 15-mer phage display peptide library was employed to pan against human rotavirus immobilized on solid phase. 4 different peptides were selected and could bind with rotavirus particles specifically. Plaque reduction neutralization test and MTT analysis results indicated that 3 of the peptides can inhibit rotavirus infecting in vitro. A peptide which sequence is QSNPIHIITNTRNHP showed the best efficiency--93% neutralization infectivity. Two other peptides, A and B, showed 40% and 50% neutralization infectivity respectively. Amino sequence analysis results indicate the 3 peptides containing 2 conserved motifs: SNPIHII and NIP. No putative trypsin hydrolysis site was found in C peptide, however, 4 and 3 potential sites were found in A and B peptides respectively. Using trypsin inhibitor, both A and B peptides showed the similar antiviral effect as that of C peptide. It suggests that the intactness of the 2 conserved motifs play an important role in counteracting virus infection. According to the results of this study, peptide C is hopeful to be exploited as an antiviral peptide drug.
Amino Acid Sequence
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Animals
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Antiviral Agents
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isolation & purification
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pharmacology
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Cell Line
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Cell Survival
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drug effects
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Dose-Response Relationship, Drug
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Drug Evaluation, Preclinical
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Humans
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Molecular Sequence Data
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Neutralization Tests
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Peptide Library
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Peptides
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chemistry
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immunology
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pharmacology
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Protein Binding
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Rotavirus
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drug effects
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growth & development
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immunology
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Sequence Analysis, Protein
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Viral Plaque Assay
7.Effect of botulinum toxin type A injection on hypertrophic scar in rabbit ear model.
Lin WANG ; Ning-Zheng TAI ; Zhi-Hong FAN
Chinese Journal of Plastic Surgery 2009;25(4):284-287
OBJECTIVETo investigate the effect of botulinum toxin type A (Botox A) injection on hypertrophic scar in rabbit ear model.
METHODSThe hypertrophic scar model was established in 16 Japanese rabbits' ears. These wounds were divided into two groups as group T (treated with Botox A, n = 48) and group S (not treated, n = 48). The wounds healing times and scar hypertrophy were observed with 8 specimen of normal skin at the rabbit ears as sham group B. HE stain was used to assess the hypertrophic index (HI). The expression of collagen I and III was tested by western-blot. The cell cycle of fibroblasts was studied by flow cytometry.
RESULTSThe HI was significantly lower in group T than in group S (P < 0.01). The expression of collagen I and III, as well as the ratio of I to III, was markedly stronger in group S than in group T (P < 0.01). Compared with group T, more fibroblasts were in G2-M in group S and fewer in G0-G1 (P < 0.05).
CONCLUSIONSLocal injection of Botox A can inhibit the formation of hypertrophic scar and the activity of fibroblasts in rabbit ear model. It can significantly decrease the expression of collagen I and III in hypertrophic scar, as well as the ratio of collagen I to III. It serves as the basis for the treatment of hypertrophic scar with Botox A.
Animals ; Botulinum Toxins, Type A ; pharmacology ; Cells, Cultured ; Cicatrix, Hypertrophic ; metabolism ; pathology ; Ear ; pathology ; Female ; Fibroblasts ; drug effects ; Injections, Subcutaneous ; Rabbits ; Wound Healing ; drug effects
8.Study on selective isolation of volatile oil in the seed of Fructus foeniculi.
Cheng WANG ; Ning-bo GONG ; Qi-tai ZHENG ; Wen-sheng GUO ; Yang LU
China Journal of Chinese Materia Medica 2003;28(3):240-242
OBJECTIVETo study the selective isolation of a single chemical component from volatile oil of Fructus foeniculi by inclucion method.
METHODThe host molecule was selected and a single chemical component isolated from volatile oil by the host-guest recognition.
RESULTX-ray single crystal analysis showed that 1,1,6,6-tetraphenylhexa-2, 4-diyne-1, 6-diol could successfully include 4-[1-propenyl] benzaldehyde from volatile oil of Fructus foeniculi.
CONCLUSIONThe host-guest inclusion technology can be used to isolate a single component selectively from mixture.
Crystallization ; Crystallography, X-Ray ; methods ; Foeniculum ; chemistry ; Molecular Conformation ; Oils, Volatile ; chemistry ; isolation & purification ; Plants, Medicinal ; chemistry ; Seeds ; chemistry
9.Comparison of bonding properties of five adhesives in primary dentin.
Yue CUI ; Zu-tai ZHANG ; Li-hua GE ; Ning DING ; Dong-mei YANG
Chinese Journal of Stomatology 2013;48(6):338-342
OBJECTIVETo evaluate the microtensile bond strength (µTBS) of five dentin adhesives and their respective fracture modes.
METHODSThe flat dentine surfaces of 75 primary teeth were randomly divided into five groups,which was treated with FL-BondII(group A), Clearfil Protect Bond(group B), Clearfil SE Bond(group C), Adper(TM) Easy One(group D), and Single Bond 2(group E) respectively. The µTBS was determined with microtensile tester and the fracture mode was observed by scanning electron microscope(SEM).
RESULTSThe mean µTBS for group A,B,C,D and E was (28.3 ± 2.2), (32.4 ± 2.5), (38.3 ± 2.8), (32.9 ± 3.4) and (23.2 ± 1.9) MPa respectively. There was significant difference between group C and group A,E (P < 0.01), and no significant difference between group C and group B,D. There was no significant difference between group A and group E (P > 0.05). The SEM indicated that there was no significant difference in the fracture mode.
CONCLUSIONSThe bonding property of Clearfil Protect Bond is equivalent to Clearfil SE Bond and Adper(TM) Easy One, superior to Single Bond 2 and more suitable for primary dentin bonding .
Adhesives ; chemistry ; Bisphenol A-Glycidyl Methacrylate ; chemistry ; Child ; Dental Bonding ; methods ; Dentin ; ultrastructure ; Dentin-Bonding Agents ; chemistry ; Denture Retention ; Humans ; Materials Testing ; Microscopy, Electron, Scanning ; Molar ; Resin Cements ; chemistry ; Surface Properties ; Tensile Strength ; Tooth, Deciduous
10.Identification of Pterocephalus hookeri.
Xin-lu GUAN ; Yu-ning YAN ; Zi-he REN ; Tai-ming WEI ; Fu-yin LI ; Ying-shan ZHANG
China Journal of Chinese Materia Medica 2004;29(11):1027-1030
OBJECTIVETo study the identification method of Pterocephalus hookeri.
METHODThe microscopical, Physicochemical and TLC methods were used.
RESULT AND CONCLUSIONThe convenient and effective identification methods for P. hookeri were established, which provide basis for its quality standard and development.
Chromatography, Thin Layer ; Drugs, Chinese Herbal ; analysis ; Magnoliopsida ; anatomy & histology ; chemistry ; Pharmacognosy ; Plant Leaves ; anatomy & histology ; chemistry ; Plant Roots ; anatomy & histology ; chemistry ; Plants, Medicinal ; anatomy & histology ; chemistry ; Quality Control