Intestinal trefoil factor(ITF)is a low molecular weight polypeptide expressed in intestine. ITF is also found in the brain,respiratory,eyes and so on.To study recombinant ITF will have an important impact for the treatment of gastrointestinal disease, respiratory tract disease, eyes disease and so on.

Postoperative pancreatic fistula (POPF) is the most common complication after pancreaticoduodenectomy (PD) and remains a challenge to pancreatic surgeons.In pancreaticojejunostomy,the methods of reconstruction,experience and operational techniques of surgeons are closely related to POPF.Based on a literature review and on personal experience,the author presented the basic requirements and principle in reconstructing a safe pancreaticojejunostomy (PJ).The traditional methods of pancreaticojejunostomy and their recent developments were evaluated,with their advantages and disadvantages compared.The indications of various types of PJ and the special skills required were summarized.According to his own experience,the author described in detail his recommended method of anastomosis.

Objective To explore the ways to strengthen the mode and effect of the standard community management of the diabetes,and the management model of diabetic mellitus that adopt to current situation of China.Methods 80 diabetics patients were divided into intensive group and common group,and the period of the treatment is three consecutive months.We observed the levels of FBG,2hPG,HbA1c and the cost of drugs.Results The levels of FBG(6.82 ± 1.23),2hPG[(9.51 ± 2.18)mmol/L]and HbA1c(7.52 ± 1.31)％ in intensive group were significantly lower than common group[(8.65 ± 2.31)mmol/L、(12.22 ± 3.11)mtmol/L,(10.32 ± 3.51)％](t =3.12,3.25,3.56,3.69,all P ＜ 0.05).The cost of drugs in intensive group[(621.5 ± 204.5)yuan]was significantly lower than common group[(796.5 ± 240.6)yuan](t =3.69,P ＜ 0.05).Conclusion The ways to strengthen the mode could spend less but control disease well,and improving the existing community diabetes management should be truly effective in reducing medical costs.

Objective To investigate the feasibility,technique and clinical significance of preserving the great auricular nerve in parotid benign tumors surgery during the first postoperative year.Methods Fifty-two patients with parotid benign tumors were randomly divded into 2 groups.24 patients (group sacrificed) underwent classic parotidectomy with sacrifice of the great auricular nerve.The surgeons spare the nerve in the 32 patients with FOCUS ultrasonic harmonic scalpel (group preserved).Results After surgery,both groups showed lower levels of subjective sensation and sensory function test at auricle.These alterations were less pronounced in group preserved.Both groups showed improvement over time.In group preserved the sensory function reached normal level by 12 months after surgery.The recuperation in group sacrificed was partial at 12 months after operation.Conclusion The great auricular nerve preservation during parotidectomy is feasible,It can decrease sensory disturbance in the early postoperative period and avoid the permanent sequelae that occur when the nerve is sacrificed.The FOCUS ultrasonic harmonic scalpel technique can reduce the operation time.

Objective To investigate the changes and clinical significance of hemorrheology and platelet parameters examination in patients with hypertension. Methods Hemorrheology and platelet parameters were detected in 78 patients with hypertension and in 72 healthy subjects as control group. Then difference were compared. Results The hemorrheology and platelet parameters in he patients with hypertension at stage Ⅰ were no different in comparison with the control group(all P＞0.05) ,which at stage Ⅱ and Ⅲ were significantly different in comparison with stage Ⅰ and the control group ( all P ＜ 0. 05). and at stage Ⅲ were significantly different in comparison with stage Ⅱ(all P＜0.05). Conclusion The changes ofhemorheology and platelet parameters were closely related with hypertension,which were helpful to the clinical diagnosis of hypertension and had important significance to the therapy, monitor and prognosis of hypertension.

OBJECTIVE: To study the effects of extracts of the seed of Litchi (ESL) on the expression of estradiol receptor (ER) and progestogen receptor (PR) in mammary tissue and apoptosis of mammary glandular cells in rats with hyperplasia of mammary glands (HMG). METHODS: The HMG model was induced by intramuscularly injecting with 0.5 mg?kg-1 estradiol benzoate for 25 days, subsequently intramuscularly injecting with 4 mg?kg-1 progesterone for 5 days. 60 healthy nonfertile SD rats were divided into normal group, model group, rujieping group and high-dose, medium-dose and low-dose ESL groups, treating with distilled water, distilled water, 3.3 g?kg-1 Rujieping and 44, 22, 11 g?kg-1 ESL via i.g. gtt once a day for 30 days, respectively. Immunohistochemical method was applied to observe the expression of ER and PR in rat mammary tissue and apoptosis of mammary glandular cells. Uterus index was also calculated. RESULTS: High-dose and medium-dose of ESL can inhibit the expression of ER and PR of mammary gland in HMG rats and induce apoptosis of mammary glandular cells(P

As a distinct clinicopathological entity, mediastinal gray zone lymphoma cannot be classified by using differential diagnostic criteria. The cases represent a spectrum of tumors having characteristics of both primary mediastinal large B-cell lymphoma (PMBL) and classic Hodgkin lymphoma (cHL). In the 2008 WHO Classification, a novel category designated B-cell lymphoma, unclassifiable, with features intermediate between diffuse large B-cell lymphoma and classical Hodgkin lymphoma (BCLu) has been created to include these neoplasms. The main features of BCLu are clearly different from those of conventional mediastinal large B-cell lymphomas. Diagnosis of BCLu requires a multiparameter approach incorporating morphological, immunophenotypic, immunohistochemical and other features. These lymphomas generally have a more aggressive clinical course and poorer outcome. There is no consensus on the optimum treatment, while the recommended therapies for aggressive B-cell lymphomas might be effective options.

Essential hypertension is a multifactorial disease. Many experimental studies have elucidated the role of oxidative stress and atherosclerosis in the pathogenesis of essential hypertension. Apolipoprotein E is a plasma protein that is found to have antioxidant properties, and it also protects against atherosclerosis. Interestingly, the biological function of apolipoprotein E is strongly affected by polymorphisms in its gene. Based on this evidence, our aim was to investigate the association of apolipoprotein E gene polymorphisms with essential hypertension. Methods: This study was conducted on 70 hypertensive patients and 73 control participants recruited from the Balok governmental health clinic in Kuantan, Pahang. The polymerase chain reaction restriction fragment length polymorphism assay (PCR-RFLP) was used for determination of the apolipoprotein E genotype. Our results were also verified later by direct sequencing of the amplicons. Results: There was no significant association of apolipoprotein E allele or genotype frequencies with hypertensive disease or blood pressure levels, although the E4 allele was slightly more frequent in the hypertensive patients than in the control group (OR=1.055; 0.471–2.359, CI 95%). To improve the precision of the study and to settle the controversies among similar studies meta-analysis was performed; however it revealed a net nonsignificant association between the apolipoprotein E4 allele with essential hypertension in the combined population. Conclusion: Our data and the meta-analysis findings provide evidence that apolipoprotein E gene polymorphism has no direct significant association with hypertension.

Objective To investigate the clinical efficacy and safety between laparoscopic and transabdomi -nal hysterectomy ,reduce the occurrence of complications measures .Methods On 154 cases of hysterectomy indica-tions were nonrandomly divided into two groups ,one group for the observation group of 79 cases of laparoscopic opera-tion,another group was control group of 75 patients with transabdominal operation;two groups had no significant differ-ence in age and disease .The clinical effect ,postoperative complications and safety between the two groups during and after operations were observed .Results The operation time of the laparoscopic group (72.0 ±13.2) min was signifi-cantly shorter than that of the control group (98.0 ±20.7)min (t=3.396,P=0.021);The bleeding quantity of the laparoscopic group(96.0 ±75.4)mL was significantly shorter than that of the control group (159.4 ±98.0)mL(t=9.680,P=0.014);The aerofluxus time of the laparoscopic group (17.2 ±4.2) h was significantly shorter than that of the control group(28.6 ±6.1)h(t=9.760,P=0.013).The average postoperative hospital stay time was significantly shorter in laparoscopic group(5.2 ±1.9)d than those in abdominal group(9.5 ±3.2)d (t=-5.025,P=0.018). The analgesic used in the laparoscopic group (3/89)was significantly shorter than that in the control group (48/78) (t=-8.820,P=0.000).Conclusion Laparoscopic hysterectomy is safe and effective .It is worth to promote in clinical,while improving the operation skills is the key to reduce the complications .

It is very important for the research nurses to announce the purpose, to prepare and transfuse the agents, to observe the therapic effects and to collect the data in clinical trials of nutrition agents. Our experiences include: ①The research knowledge of observing nurses must be trained. ②The management system should be standard and specific. ③The health education should be careful. ④The dose of testing agents must be precise. ⑤The sample collection must be correct.