Intestinal trefoil factor(ITF)is a low molecular weight polypeptide expressed in intestine. ITF is also found in the brain,respiratory,eyes and so on.To study recombinant ITF will have an important impact for the treatment of gastrointestinal disease, respiratory tract disease, eyes disease and so on.

Postoperative pancreatic fistula (POPF) is the most common complication after pancreaticoduodenectomy (PD) and remains a challenge to pancreatic surgeons.In pancreaticojejunostomy,the methods of reconstruction,experience and operational techniques of surgeons are closely related to POPF.Based on a literature review and on personal experience,the author presented the basic requirements and principle in reconstructing a safe pancreaticojejunostomy (PJ).The traditional methods of pancreaticojejunostomy and their recent developments were evaluated,with their advantages and disadvantages compared.The indications of various types of PJ and the special skills required were summarized.According to his own experience,the author described in detail his recommended method of anastomosis.

Objective To explore the ways to strengthen the mode and effect of the standard community management of the diabetes,and the management model of diabetic mellitus that adopt to current situation of China.Methods 80 diabetics patients were divided into intensive group and common group,and the period of the treatment is three consecutive months.We observed the levels of FBG,2hPG,HbA1c and the cost of drugs.Results The levels of FBG(6.82 ± 1.23),2hPG[(9.51 ± 2.18)mmol/L]and HbA1c(7.52 ± 1.31)％ in intensive group were significantly lower than common group[(8.65 ± 2.31)mmol/L、(12.22 ± 3.11)mtmol/L,(10.32 ± 3.51)％](t =3.12,3.25,3.56,3.69,all P ＜ 0.05).The cost of drugs in intensive group[(621.5 ± 204.5)yuan]was significantly lower than common group[(796.5 ± 240.6)yuan](t =3.69,P ＜ 0.05).Conclusion The ways to strengthen the mode could spend less but control disease well,and improving the existing community diabetes management should be truly effective in reducing medical costs.

Objective To investigate the feasibility,technique and clinical significance of preserving the great auricular nerve in parotid benign tumors surgery during the first postoperative year.Methods Fifty-two patients with parotid benign tumors were randomly divded into 2 groups.24 patients (group sacrificed) underwent classic parotidectomy with sacrifice of the great auricular nerve.The surgeons spare the nerve in the 32 patients with FOCUS ultrasonic harmonic scalpel (group preserved).Results After surgery,both groups showed lower levels of subjective sensation and sensory function test at auricle.These alterations were less pronounced in group preserved.Both groups showed improvement over time.In group preserved the sensory function reached normal level by 12 months after surgery.The recuperation in group sacrificed was partial at 12 months after operation.Conclusion The great auricular nerve preservation during parotidectomy is feasible,It can decrease sensory disturbance in the early postoperative period and avoid the permanent sequelae that occur when the nerve is sacrificed.The FOCUS ultrasonic harmonic scalpel technique can reduce the operation time.

Objective To explore the effect of naphthalene acetic acid(NAA) on animal cells. Methods K562 cells were treated in vitro with different doses of NAA (0, 50 and 100?mol/L). The cell proliferation, cell cycle, cell division index and apoptosis was observed by means of MTT, cell enumeration, and flow cytometry. The sister chromatid exchange (SCE) and micronucleus were tested. Results The proliferation of K562 cell was inhibited by NAA compared with that of the controls; the cell cycle was arrested at G1, and the cell division index reduced; apoptosis cells increased lightly in NAA experiments; the frequency of SCE and micronuclear formation were increased by NAA treatment. Conclusion NAA has antiproliferative activities on K562 cell.

Objective To investigate clinical efficacy of the NB-UVB phototherapy combined with calcipotriol ointment and halometasone cream in the treatment of psoriasis.Methods 90 patients with psoriasis were selected and randomly divided into control group and observation group with 45 cases of patients.In both two groups,calcipotriol ointment,1 time/d,were used combined with halometasone cream,1 time/d,with a total of 4 weeks.On the basis of these,patients of observation group were added NB-UVB phototherapy (Germany Waldmann7001K ultraviolet light therapy device),1 time every other day with starting dose of 0.5J/cm2,each increment of 0.1J/cm2,for treatment of 15 times.Before and after treatment in patients of two groups,PASI score and clinical efficacy were observed.Visual analogue scale (VAS) were was used to observe itching skin lesions before and after treatment.By using Dermatology Life Quality Index (DLQI),qualities of life in two groups of patients before and after treatment were evaluated.observe two patients before and after treatment expression levels of serum IL-17A,IL-22 and IL-1βwere also observed in patients of the two groups.Results The total efficiency of the observation group was 88.89％,which of the control group was 80.00％,the clinical efficacy of the observation group which was higher than that of the control group,and the difference was statistically significant (x2 =5.23,P ＜ 0.05).Before treatment,the difference of PASI,VAS score and DLQI in the two groups of patients were not statistically significant (P ＞ 0.05).After treatment,scores in the two groups of patients were significantly decreased,and the degree of decrease in the observation group was higher than the control group,the difference was significant (P ＜ 0.05).Before treatment,the differences in serum IL -17A,IL-22 and IL-1β expression levels of both two groups were not statistically significant (P ＞0.05).After treatment,these expression levels of the two groups of patients decreased significantly,and the decreased of observation group was more significantly than that of the control group (P ＜ 0.05).Conclusion NB-UVB phototherapy combined with calcipotriol ointment and halometasone cream in treatment of psoriasis vulgaris patients can significantly improve patients' skin lesions,and can improve patients' quality of life score,which is worthy of promotion.

With the development of perinatal medicine,the survival rate of preterm infants has risen these years,and the incidence of bronchopulmonary dysplasia (BPD) has also increased as a result.BPD is a chronic lung disease which is caused by multiple factors,It has complicated etiology and pathogenesis,high mortality early after birth,and high incidence of later adverse outcomes in respiratory and nervous systems,which seriously affect the survival rate and quality of life in preterm infants.This article will summarize the current situation in prevention and treatment of BPD.

Objective:To explore therapeutic effects of trimetazidine combined atorvastatin calcium on chronic heart failure (CHF) .Methods:According to random number table ,a total of 80 CHF patients were randomly and equally divided into routine treatment group and combined treatment group (received trimetazidine and atorvastatin calcium based on routine treatment group) .NYHA class improvement ,6min walking distance (6MWD) ,left ventricular ejection fraction (LVEF) , left ventricular end‐systolic dimension (LVESd ) , plasma levels of brain natriuretic peptide (BNP ) , C reactive protein (CRP) and adverse drug reactions (ADR) were compared between two groups before and six months after treatment .Re‐sults:Compared with routine treatment group after treatment ,there was significant rise in total effective rate of heart function improvement (77.5% vs .95.0% ) in combined treatment group , P=0.023. Compared with routine treatment group ,there were significant rise in 6MWD [ (346.6 ± 47.3) m vs .(396.4 ± 49.2) m] and LVEF [ (44.5 ± 4.8)% vs . (49.3 ± 5.4)% ] ,and significant reductions in LVESd [ (54.2 ± 4.1) mm vs .(51.1 ± 4.3) mm] ,plasma levels of BNP [ (348.4 ± 61.3) pg/ml vs .(251.4 ± 53.6) pg/ml] and CRP [ (9.1 ± 2.2) mg/L vs .(6.4 ± 1.6) mg/L] in combined treatment group , P<0.05 or <0.01. No severe ADR was found in two groups .Conclusion:Trimetazidine combined ator‐vastatin calcium can significantly improve cardiac function and exercise capacity ,improve quality of life and ventricular re‐modeling in CHF patients ,it's safe and reliable ,which is worth extending .

In order to provide highly purified preparation of human ieukoregulin (LR) for the exploration of its anticancer mschatiism, crude preparations of LR and lymphotoxin(LT) derived from human spleen cell cultures induced with phytohem-agglutinin and diterp;ne ester l2-O-tetradecanoylphorbol-i3-acetate were purified by using four purification nmhois : (a) Con A-Sepharose 48 affinity chromatography, by which crude human LR or LT was purified to maximum specific activity of 85 333 U/mg protein or 13333 U/mg protein with a recovery of 28.1% or 31.9%, respectively 5 (b) DEAE-Sjphadex A-50 ion-exchange chromatography, by which partially purified LR or LT obtained after Con A-Sepharose 4B affinity chromatography was further purified 2.44-fold or 3.8o-fold with a recovery of 96% or 92%, respectively ; (c) DEAE-22-celluiose ion-exchange chromatography, by which crude human LR or LT was purified to maximum specific activity of 11 294 U/mg protein or 13 176 U/mg protein with a recovery of 87.5% or 89.6%.respectively ; (d) Blue-Sepharose CL-6B affinity chromatography, by which crude human LR or LT was purified to maximum specific activity of 51.89 U/mg protein or 2594 U/mg protein with a recovery of 53.75% or 5t3.G7%, respectively.Our data indicate that Con A-Sepharose 4B affinity chromatography, DEAE-Sephadex A-50 ion-exchange chromatography.DEAE-22-cellulose ion-exchange chromatography and Blue-Sepharose CL-6B affinity chromatography may be useful in the purification of LR or LT.

OBJECTIVE: To study the effects of extracts of the seed of Litchi (ESL) on the expression of estradiol receptor (ER) and progestogen receptor (PR) in mammary tissue and apoptosis of mammary glandular cells in rats with hyperplasia of mammary glands (HMG). METHODS: The HMG model was induced by intramuscularly injecting with 0.5 mg?kg-1 estradiol benzoate for 25 days, subsequently intramuscularly injecting with 4 mg?kg-1 progesterone for 5 days. 60 healthy nonfertile SD rats were divided into normal group, model group, rujieping group and high-dose, medium-dose and low-dose ESL groups, treating with distilled water, distilled water, 3.3 g?kg-1 Rujieping and 44, 22, 11 g?kg-1 ESL via i.g. gtt once a day for 30 days, respectively. Immunohistochemical method was applied to observe the expression of ER and PR in rat mammary tissue and apoptosis of mammary glandular cells. Uterus index was also calculated. RESULTS: High-dose and medium-dose of ESL can inhibit the expression of ER and PR of mammary gland in HMG rats and induce apoptosis of mammary glandular cells(P