It is very important for the research nurses to announce the purpose, to prepare and transfuse the agents, to observe the therapic effects and to collect the data in clinical trials of nutrition agents. Our experiences include: ①The research knowledge of observing nurses must be trained. ②The management system should be standard and specific. ③The health education should be careful. ④The dose of testing agents must be precise. ⑤The sample collection must be correct.

The portable dynamic electrocardiosignal data collector is the important part in the dynamic ECG analysis system.In this paper,the writer introduces the principle of intelligent data collector system,which takes microcontroller as core,and discusses the points of hardware design and the arithmatic of data pretreatment software.

Objective To investigate the resistance of clinical isolates of P.aeruginosa in China during 2005.Methods Clinical isolates of P.aeruginosa from 7 teaching hospitals in China were collected and antimicrobial susceptibility were tested by Kirby-Bauer method.Results A total of 2 323 clinical isolates of P.aeruginosa were collected.The resistance rates of P.aeruginosa to antimicrobial agents from low to high were amikacin(22.5%),cefoperazone-sulbactam(22.8%),cefepime(27.6%),ceftazidime(28.9%),imipenem(31.3%),ciprofloxacin(31.7%),meropenem(33.7%),piperacillin-tazobactam(34.4%),aztreonam(36.8%),piperacillin(44.2%),ticarcillin-clavulanic acid(50.9%).Multi-drug resistant(MDR)and pan-drug resistant(PDR)strains accounted for 8.4% and 4.2%,respectively.Some(36.7%-53.4%)of the strains resistant to other antimicrobial agents were still susceptible to amikacin.Therefore,combination therapy of ?-lactams plus an aminoglycoside is usually indicated for serious infections caused by P.aeruginosa.By 'interpretative reading' of the susceptibility pattern of 'predictive drugs',we can predict the underlying resistance mechanisms.Conclusions The surveillance data indicate that the resistance of P.aeruginosa in China is relatively serious and its resistance mechanisms are very complicated.Close attention should be paid to this problem by physicians and clinical laboratory.

AIM: To compare the efficacy and safety of oxiracetam and donepezil in treating Alzheimer disease with mild and moderate MMSE scores.METHODS: 68 patients were randomly divided into oxiracetam group(p.o.,n=34) and donepezil group(p.o.,n=34).The MMSE and Blessed-Roth measuring scales were used to evaluate the curative effects before treatment and three months after treatment.RESULTS: There were significant differences between the indexes expect for activities of daily living before and after treatment in two groups(P0.05).CONCLUSION: The curative effect and safety of oxiracetam and donepezil in treating mild and moderate Alzheimer disease are similar.

Differential treatment based on signs and symptoms is the special points of TCM,the identification of syndrome is the basis of differential treatment.Scientific research on syndrome plays a leading role in unifying pathogenesis of dyslipidemia,and certainly improves the research level of dyslipidemia in TCM.According to the status quo of lipid abnormality research,the research in this field can be conducted in two aspects in priority:firstly,the research of subjective symptoms can provide some basis for TCM research;secondly,the associativity of TCM syndromes and the metabolic syndromes is advantageous for exploring the nature and rules of lipid abnormality in TCM,it will be helpful for the improvement of lipid abnormality differentiation and treatment.

Objective To investigate the clinical features,pathological features and prognostic factors of young patients with endometrial cancer.Methods One hundred and ten cases of endometrial cancer under 40 in Jiangning Hospital Affiliated to Nanjing Medical University from January 1st 2008 to January 1st 2012 were selected as the study group while one hundred and ten cases of cervical cancer over 40 in the same period as the control group.The clinical features and pathological features were compared between the two groups.Univariate and multivariate analysis were used to determine the prognostic factors.Results The occurrence rate of abnormal vaginal bleeding (74.5% (82/110)),vaginal drainage (22.7% (25/110)) and infertility (38.2% (42/110)) in the study group were significantly lower than those in the control group (59.1%(65/110),4.5%(5/110),3.6%(4/110),χ2=4.30,12.23,16.53,P<0.05),while the production time ((1.2±0.6) times),combination of hypertension (2.7% (3/110)) and diabetes (1.8% (2/110)) were significantly lower than those in the control group ((2.3±0.9)times,14.5% (16/110),10.0%(11/110),t=-5.69,χ2=23.61,8.26,P<0.05).The histological classification of the two groups was mainly based on endometrioid adenocarcinoma,and the pathological types were mainly based on adenocarcinoma,the differences were not statistically significant (χ2=0.75,1.56,P>0.05).There were differences in histology,PR,muscle,and lymph node metastases (χ2=5.39,4.29,4.89,P<0.05).Multivariate analysis of prognostic factors in young patients with endometrial cancer showed that histology,PR and muscle metastasis were independent risk factors for the prognosis of young endometrial cancer patients.Conclusion There are differences in clinical features and pathological features between young and elderly endometrial cancer patients.The census and prevention among young women should be strengthened and the individualized treatment and the prevention and control system should be upgraded.

Sepsis is one of the most important causes of death in critically ill children.The leading cause of sepsis is bacterial infection.The early diagnosis of bacterial infection allows initiation of appropriate antimicrobial therapy that strongly correlates with positive outcomes.At present,the methods of detection and identification of bacteria in our country are still at the level of blood culture and biochemistry,and they have the weakness of time-consuming process and lower positive rate.Therefore,it is very important to establish a rapid and sensitive diagnostic method for early diagnosis of bacterial infection.In recent years,with the rapid development of molecular detection technology,some new molecular biological technologies can not only improve the detection accuracy and sensitivity,but also reduce the detection hours and expand the detection pathogen spectrum.Nowadays,they have been applied to bacteria classification and identification.There are two kinds of molecular biological technology that are applied at present.One is based on nucleic acid detective technology,such as nucleic acid amplification,DNA sequencing,gene chips,etc.The other is based on proteome technologies,such as biological mass spectrometry.In this paper,the application of recent molecular biological techniques in the rapid detection of bacteria is reviewed.

Objective To reduce the recurrence rate and improving the hearing recovery effect in chronic otitis media patients with obstructive lesions in the attic, through the low bone intact-bridge tympanomastoidectomy.Methods A total of 23 patients with little severe or severe localized attic lesions granulation wrapped in ossicular chain blocking the entrance of tympanic antrum or affecting the attic drainage were included in this study.We have modified the intact-bridge tympanomastoidectomy to low bone intact-bridge tympanomastoidectomy (At the tympanic antrum entrance and lateral attic wall, we retained a more narrow low bone bridgestructure), completely cleared the localized lesions, released the activities limited ossicular chain and unobstructed the attic and middle tympanum drainage channel.And patients were followed up for 0.5~3 years.Results Before operations, the average pure tone auditory hearing threshold of all 23 patients was 43.91±9.90 dB HL,the preoperative air-bone gap was 24.04±5.10 dB.While the postoperative stable pure tone hearing test results were 33.17±7.63 dB HL and 14.70±4.52 dB, respectively.All patients had postoperative dry ear within 3 months.There was no recurrence of tympanic membrane perforation during follow-up.One patient had tympanic effusion(secretory otitis media) had accepted grommet insertion, 6 months after T-tube insertion, the tube was removed, and was followed up to 1 year.Conclusion The low bone intact-bridge tympanomastoidectomy by preservation of low bone bridge used in patients with chronic otitis media and obstructive lesions in the attic middle tympanum could reduce the recurrence rate and improve the recovery of hearing.

The scaffold material is one of the most key topics about the study of bone tissue engineering. The scaffold material of bone tissue engineering is divided into natural biomaterials and artificial synthesized biomaterials. Each kind of the materials includes organic materials and inorganic materials. Each scaffold material has its virtues and shortages. Although the existing scaffold materials merely carry some effects of the ideal materials require, the optimal scaffold may be designed using the necessary evidences provided by analyzing their virtues and shortages. This article summarized the study about the tissue engineering scaffold materials, such as porcelain and ceramics material, polymers material, natural organism-derived material and composite material.

Objective To explore the application of BCG polysaccharide nucleic acid injection in the treatment of skin diseases.Methods Selected in June 2015 to August 2016 period, in our hospital for skin disease treatment of 200 patients. According to the type of skin disease is divided into chronic eczema, chronic urticaria, alopecia areata and flat warts four groups, each group contains 50 patients. And then each group of patients were randomly divided into two groups, one group as a control group, a group as the observation group, each group of 25 cases. The control group was treated with traditional drug therapy, and the observation group needed to increase the use of BCG polysaccharide nucleic acid injection. The treatment period is for three months. To observe and compare the treatment effect of the control group and the experimental group after three months, that is, to observe and compare patients with chronic eczema, chronic urticaria, alopecia areata and flat warts of the four different types of skin diseases after three months of treatment effect. Results In the patients with chronic eczema, the effective rate of treatment was 96.0％ in the observation group and 68.0％ in the control group. The effective rate of the treatment group was significantly higher than that of the control group (P<0.05). The effective rate was 88.0％ in the observation group and 60.0％ in the control group. The effective rate of the treatment group was significantly higher than that of the control group (P<0.05). In the patients with alopecia areata, The effective rate of treatment was 84.0％ in the observation group and 72.0％ in the control group. The effective rate of the treatment group was significantly higher than that of the control group (P<0.05). In the patients with flat warts, The effective rate was 92.0％ and the effective rate was 68.0％ in the control group. The effective rate of the treatment group was significantly higher than that of the control group (P<0.05). In the different types of skin diseases, the effective rate of treatment was obvious Higher than the control group, the treatment effect was significantly better than the control group (P<0.05). Conclusion For patients with skin diseases using BCG polysaccharide nucleic acid injection for treatment, compared to the traditional drug treatment better. For the different types of skin diseases, the use of BCG polysaccharide nucleic acid injection for treatment can get the best therapeutic effect, so BCG polysaccharide nucleic acid injection in different types of skin disease clinical treatment is a worthy of use and Its promotion of the treatment.