Nuclear magnetic resonance (NMR) spectroscopy can be used to both identify and quantify chemicals from complex mixtures. Over the last several decades, significant technical and experimental advances have made quantitative nuclear magnetic resonance (qNMR) a valuable analytical tool for quantitative measurements of a wide variety of samples. This particular approach is now being exploited to characterize the metabolomes of many different biological samples and is called quantitative metabolomics or targeted metabolic profiling. In this review, some of the strengths, limitations of NMR-based quantitative metabolomics will be discussed as well as the practical considerations necessary for acquisition with an emphasis on their use for bioanalysis. Recent examples of the application of this particular approach to metabolomics studies will be also presented.

ObjectiveTo evaluate the effect of compound oxymatrine injection combination with chemotherapy in patients with advanced ovarian carcinoma.MethodsEighty-six patients with advanced ovarian carcinoma were divided by random digits table method into observation group(43 cases) receiving compound oxymatrine injection plus chemotherapy(paclitaxel plus carboplatin) and control group (43cases) treated with chemotherapy(p aclitaxel plus carboplatin) alone.The change of white blood cell,adverse reaction and effect of the patients were observed.ResultsThe white blood cell of observation group after treatment was obviously higher than that of control group [ (4.7 ± 1.4) × 109/L vs.(3.2 ± 1.6) × 109/L,P ＜ 0.05 ].The rates of nausea and vomiting,transaminase elevating and kidney damage of observation group were obviously lower than those of control group[ 16.3％ (7/43),14.0％ (6/43),20.9％ (9/43) vs.34.9％(15/43),32.6％ (14/43),44.2％ ( 19/43 ),P ＜ 0.05 ].The complete remission rate of observation group was significantly higher than that of control group[55.8％(24/43) vs.27.9％(12/43),P ＜ 0.05].Conclusion Addition of compound oxymatrine injection to chemotherapy for patients with advanced ovarian carcinoma can effectively protect patients against the decreasing of the white blood cell and attenuate the side effects brought by chemotherapy.

ObjectiveTo investigate the effect of humanized nursing in ovarian cancer patients to improve treatment compliance and negative emotional state. Methods120 cases of ovarian cancer patients from October 2009 to October 2010 in our hospital were chosen as the research object.They were divided into the control group and the observation group according to randomization methods with 60 cases in each group.The control group patients were taken routine care.The patients in the observation group were taken humanized nursing on the basis of routine care.The treatment compliances during treatment were compared.The anxiety,depression scores for the two groups of patients before and after treatment were compared.Based on the three indicators,the effect of humanized nursing in ovarian cancer patients to improve treatment compliance and negative emotional state was evaluated. ResultsThe treatment compliance during treatment for the two groups was compared,the full compliance of patients of the observation group was significantly higher than the control group.The difference between the two groups was statistically significant.The anxiety,depression scores before treatment for the two groups were compared.There was no significant difference between the two groups.The anxiety and depression scores showed significant improvement after the treatment,but improvement level of the observation group was significantly higher than the control group.The difference between the two groups was statistically significant. ConclusionsHumanized nursing can improve the treatment compliance and negative mood of ovarian cancer patients effectively,it is conducive to the rehabilitation of patients,and is worthy of clinical application.

Objective To investigate the correlation of serum alpha-fetoprotein (AFP) with clinical pathological factors and prognosis of hepatocellular carcinoma ( HCC ).Methods Two hundred and twenty patients with HCC who hospitalized in our hospital from January 2007 to June 2009 were enrolled in this study.The relationship of AFP levels before operation to clinical pathological factors such as pathological grade,tumor size,tumor encapsulation,tumor numbers,portal vein tumor thrombus,TNM stage and postoperative recurrence were analyzed.Results Serum AFP concentration before operation were related with tumor size ( x2 =11.76,P ＜ 0.05 ),tumor encapsulation ( x2 =17.07,P ＜ 0.05 ),portal vein tumor thrombus ( x2 =8.944,P ＜ 0.05 ),higher TNM stage ( x2 =4.929,P ＜ 0.05 ) and early postoperative recurrence rate ( x2 =5.569,P ＜ 0.05 ).However,it had no correlation with pathological edmondson grade tumor numbers.Conclusion HCC patients with high serum AFP concentration often has greater tumor without encapsulation,high occurrence rate of portal vein tumor thrombus and higher TNM stage,they are more likely to early postoperative recurrence.Serum AFP before operation is useful not only for diagnosis,but also as a prognostic biomarker to predict prognosis and recurrence.

The mechanisms underlying early neurological deterioration (END) after ischemic stroke are not clear.There are no reliable predictive factors and effective preventive measures for END.The glutamatemediated excitotoxicity plays a very important role in the cascade reaction of ischemic events.High level of glutamate in plasma is one of the important predictors for END.Studies have shown that the polymorphism in the promoter of the excitatory amino acid transporter-2 gene is a potential cause for individual susceptibility to END.Some therapeutic strategies of interrupting the glutamatergic pathways may be as the strategies of intervention END.

[Objective] To compare the clinical cure effects of different operations on myomectomy, and screen out best clinical treatment for hysteromyoma in child-bearing period. [Method] Trace back and analyse 200 cases of such patients, divide them into laparotomy group, laparoscope group, vaginal group and UAE group under different therapies, compare cure effect analysis. [Result] On operation time, operation bleeding and evacuation time, the UAE group ,vaginal and laparoscope groups were successively better than the laparotomy group; but on post-operation medication rate and average in-hospital days, the UAE group was successively less than laparoscope, vaginal and laparotomy groups. On complication rate,the laparoscope was successively less than UAE, vaginal and laparotomy groups.[Conclusion] 4 therapies have respective advantages, UAE and laparoscope myomectomy have little trauma, quick re-covery and reliable cure effect, however, laparotomy can't be completely replaced owing to its wide indications.

Objective: Through summarizing the main problems and challenges faced in the medical health system reform in China , suggestions are given: the government needs to erhance the health investment and play the role of market mechanism , make the use of electionical information technique, further change the community system of implementing medicine community system, implement the medical service supervision system.

Objective To investigate the effect of mifepristone on the expression of β-endorphin in the ectopic focus of adenomyosis.Methods The expression of β-endorphin was measured by the immunochemistry method.Results During proliferative phase and secretory phase,the β-endorphin of mild dysmenorrhea were higher than that of midrange dysmenorrhea(t=2.672,2.711,all P＜0.05),the β-endorphin of midrange dysmenorrhea was higher than that of severe dysmenorrhea(t=2.341,2.365,all P＜0.05),the difference was significant.After being cured by mifepfistone,the expression of β-endorphin in the ectopic focus of adenomyosis was higher than that of control group (t=2.478,2.356,all P＜0.05),still lower than that of normal endometrial tissue,(t=2.123,2.233,all P＜0.05),the difference was significant.Conclusion The expression of β-endorphin in the ectopic focus of adenomyosis decreased significandy accompanied with the aggravation of the dysmenorrhea,and the mifepfistone could upregulate the β-endorphin and relieve the dysmenorrhea.

Objective To investigate the correlation between the expression of P-SAPK/JNK and neuronal apoptosis in the striatum during permanent middle cerebral artery occlusion (pMCAO) in rats.Methods Fifty-four male Sprague-Dawley rats were randomly divided into sham operation group and pMCAO 1-,3-,6-,12-,and 24-hour groups (n =9 in each group).Apoptotic neurons in the striatum during cerebral ischemia were detected by TUNEL assay,the nuclear translocation of P-SAPK/JNK in the striatum by immunohistochemical staining expressions of P-SAPK/JNK protein by Western blot.Results The numbers TUNEL- and P-SAPK/JNK-positive cells in the striatum at 1 hour after pMCAO increased significantly (P =0.000 1),and reached the peak at 6 hours.The numbers of TUNEL-positive cells decreased at 12 hours,however,it still higher than the sham operation group (P =0.000 2).Western blot analysis showed that the expression of P-SAPK/JNK after pMCAO increased significantly,and the time-course change was in accord with the result of immunohistochemical staining Neuronal apoptosis in the striatum was significantly positively correlated with the expression of P-SAPK/ JNK (r =0.984,P =0.000 4).Conclusions Cerebral ischemia may induce neuronal apoptosis in the striatum through the activation of P-SAPK/JNK.

Intracranial atherosclerotic stenosis mostly occurs in Asians,blacks and Hispanicsis,which is the most important reason for the occurrence and recurrence of ischemic stroke.The current studies mainly concentrate on the aspect of the relationship between the intracranial atherosclerotic stenosis and the traditional risk factors.With the development of genetic technology,the relationship between the genetic factors and intracranial atherosclerotic stenosis has also received increasing attention.This article reviews the advances in research on the traditional risk factors for intraeranial atherosclerotic stenosis and genetic research.