Objective To investigate the effects of combined treatment of suberoylanilide hydroxamic acid(SAHA)and TNF?related apoptosis inducing ligand(TRAIL)on proliferation and morphology change of triple?negative breast cancer cell MDA?MB?231. Methods The effects of combination treatment of SAHA and TRAIL on proliferation and morphology change of MDA?MB?231 cells were monitored by RTCA. Morphology changes of MDA?MB?231 cells by different treatment factors were observed through time?lapse live cell imaging acquisition. Results Real?time cell proliferation assays showed that a synergistic effect were found when MDA?MB?231 cells were treated with combination of SAHA and TRAIL , and reached the best effect with 5μmol/L SAHA and 50 ng/mL TRAIL. The results of time?lapse live cell imaging acquisition showed that the growth inhibition of MDA?MB?231 cells with combined treatment of SAHA and TRAIL were more obvious than that with treatment of SAHA or TRAIL alone. Conclusion The combined treatment of SAHA and TRAIL induces a synergistic effect on growth inhibition in triple?negative breast cancer cell line MDA?MB?231.

Objective To investigate the clinical rating scale appropriate to evaluate neurological impairment of ischemic stroke subtypes classified by Trial of Org 10172 in Acute Stroke Treatment (TOAST) criteria. Methods 289 cases of hospitalized patients with ischemic stroke in neurological wards of the first affiliated hospital of Dalian medical university from December 1,2007 to December 31, 2009 were analyzed prospectively. Results The component ratio of ischemic stroke subtypes on the basis of TOAST etiological classification were as follows: UND (36.6%) was the largest, SAO (29.8%) followed,OC (1.7%) was the lowest. In three-month mortality, CE (15.5%) was the most, LAA (11.8%) followed, SAO (1.2%) was the lowest. In three-month relapse rate, LAA (17.6%) was the most, CE (8.6%) followed, SAO (2.4%) was the lowest. The best validity of scale assessment about LAA, CE,UND, SAO subtypes were MESSS (-0.812), ESS (0.816), NIHSS (-0.807), NIHSS (-0.795) respectively, both reliability and operability were set as medium. Conclusion In ischemic stroke subtypes classified by TOAST criteria, UND had the highest incidence (SAO has the highest incidence in the specific etiology), CE mortality was the highest, LAA had the highest rate of recurrence, SAO mortality and relapse rate were the lowest. In ischemic stroke subtypes classified by TOAST criteria, MESSS was the most suitable scale for LAA, the most appropriate scale for CE was ESS, the most appropriate scale for UND, and the SAO was NIHSS.

Objective To explore mechanism of epigallocatechin-3-gallate (EGCG) on improvement of cognitive function and alleviation of hippocampal insulin resistance in APP/PS 1 transgenetic mice. Method 12 months old female APP/PS 1 mice were randomly divided into 3 groups:model group(Tg), EGCG low dose group (Tg/EGCG-L), high dose group(Tg/EGCG-H). C 57 BL/6 J mice were utilized as control. learning and memory ability in 4 group mice were detected by morris water maze test(MWM). The hippocampal TNF-α/JNK signal and IRS-1 pSer 312 expression were detected by Western blot and immunohistochemical staining. Results Compared with NT mice, Tg mice showed a marked prolongation of the escape latency and swimming distance in the MWM test(P<0.05);Abnormal activation of TNF-α/JNK signaling and increased IRS-1 pSer 312 expression in the hippocampus of Tg mice(P<0.05). EGCG-treated Tg mice showed significantly improvement of all these abnormal changes(P<0.05). Conclusion EGCG treatment is able to alleviate hippocampal insulin resistance and improve cognitive function in the APP/PS 1 mice. which may be partly attributed to the reduction of TNF-α/JNK signaling activity in this AD mouse model.

BACKGROUND: Because of the special structure of enamel fluorosis, the traditional bonding adhesives often lower the bonding strength and increase bracket loss rate, which affected the therapeutic effects seriously.OBJECTIVE: To observe the clinical bracket loss rate differences among the 3 different bonding adhesives in treating moderate and severe dental fluorosis.METHODS: A total of 15 cases (total 288 teeth) with dental fluorosis were selected, including 6 males and 9 females, aged 13-26 years. All cases were randomly divided into 3 groups, and treated by using Beijing-Tianjin enamel adhesive bonding brackets,photo-curing-flowable Resin + Single Bond 2 adhesive bonding brackets and a small amount of dental fluorosis of enamel surfaces were ground and bonding bracket, the loss rate of brackets were observed.RESULT AND CONCLUSION: The total loss rates of the Beijing-Tianjin-enamel adhesive bonding bracket group, photo-curing flowable resin + Single Bond 2 adhesive bonding bracket group, and a small amount of dental fluoresis of enamel surfaces were ground and bonding bracket group were 65.6%, 20.5%, and 2.5%, respectively. There was significant difference in bracket loss rate among bonding adhesives (P< 0.01). It suggested that a small amount of surface enamel were ground and bonding bracket got the best effect, followed by photo-curing fiowable resin + Single Bond 2 adhesive bracket bonding group, both of them were significantly better than the Beijing-Tianjin-enamel adhesive bracket bonding group. Because veneering directly requires to grind part of the tooth structure which is difficult to accept for some patients, therefore, under the premise of not destroying the tooth structure, using photo-curing fiowable resin + Single Bond 2 adhesive bonding of brackets instead of the traditional Beijing-Tianjin enamel adhesive bonding of brackets can effectively reduce the loss rate of brackets for moderate and severe dental fluorosis.

To observe the clinical effects of Depulpin in deciduous teeth. It was suggested that Depulpin was an effective and safe drug for pulp devitalizer in children.

Objective To investigate the correlation between self-nursing and the quality of life,thereby provide the basis of enhancing the patients' quality of life.Methods A total of 119 patients with type 2 diabetes metillus (T2DM) in the People's Hospital of Changyi City,Shandong Province,were selected,then they were investigated with Exercise of Self-care Agency Scale (ESCA) and Diabetes Specific Quality of Life Scale (DSQL).The correlations between self-nursing and the quality of life were analyzed.Results The self-nursing total score in 118 efficient patients was (108.36±21.94),and the low level,intermediate level and high level accounted for 2.54％ (3/118),64.41％ (76/118) and 33.05％ (39/118),respectively.The total score of DSQL was (69.95±9.74),among all factors,physiology and mentality played a significant role in the quality of life.According to Pearson correlation analysis,the self-nursing ability and the quality of life was positively correlated.Conclusions In the process of nursing intervention to T2DM,nurses should value the improvement of self-nursing ability,solidify the correct awareness of T2DM,eventually to elevate the quality of life.

Tumor necrosis factor-related apoptosis-inducing ligand(TRAIL) is a new anti-tumor biological agents which is very hot in recent years through its death receptor-induced apoptosis of tumor cells,and non-toxic to normal cells,and has synergy with the chemotherapy drug,but there is also resistance mechanisms.This article will review the biological characteristics of TRAIL and its receptors,TRAIL-induced apoptosis of tumor cells,as well as the mechanism of TRAIL in leukemia in the progress of treatment.

Corin is a newly identified membrance protease which responsible for converting pro-B-type natriuretic peptide to biologically active B-type natriuretic peptide.B-type natriuretic peptide play an important role in regulating blood pressure and cardiac function.It is a biological marker of heart failure.So Corin plays a role in reducing blood volume, blood pressure, regulating body fluid balance, and improving cardiac function.Corin may be used as a biomarker for heart failure or other cardiovascular disease.Recent studies have found that Corin is associated with the occurrence and development of cardiovascular diseases, such as heart failure and acute coronary syndrome.The article mainly expound the biological characteristics of Corin, testing methods and its clinical application in cardiovascular disease status.

Professor XUE Bo-shou skills in treating internal stubborn disease by ancient prescriptions.This article lists XUE Bo-shou treating reactive arthritis,seborrheic alopecia,chloasma,lung cancer by Huangqi Chifeng decoction.His clinic experience can be used as references by clinic practice.

Objective To investigate the association between the polymorphisms of apolipoprotein M gene (ApoM) and the risk for cerebral infarction in Han population in northern China.Methods The polymorphisms of ApoM gene were identified by PCR-DNA sequencing analysis and were subsequently detected by PCR-RFLP in 560 patients with cerebral infarction and 550 control subjects in northern China. Statistic analysis was conducted using the SPSS 10.0 program. The linkage disequilibrium (LD) was analyzed by EH and SHEsis softwares. Results Three single nucleotide polymorphisms were found in intron 1 and intron 5 of ApoM gene. The frequencies of GA+AA genotype and A allele of rs805264, GT +TT genotype and T allele of rs707922, CA+AA genotype and A allel of rs707921 were significantly higher in the patients (33.8% and 17.9%, 34.5% and 18.3%, 33.4% and 17.8%, respectively) than in controls (21.1% and 11.2%, 21.8 % and 11.5%, 20.9% and 11.1%, respectively). The LD was found in rs805264, rs707922 and rs707921 of ApoM gene (χ2=2595.03, P<0.01). There was strong LD between each pair of the three markers (D′=0.972 to 0.992). Multiple logistic regression analysis revealed that the A-T-A haplotype of the ApoM gene was an independent risk factor for cerebral infarction (OR=1.780;95%CI=1.333-2.376, P<0.01). Conclusions GA genotype and A allele of rs805264, GT genotype and T allele of rs707922, CA genotype and A allele of rs707921 may be genetic risk factors for cerebral infarction. A-T-A haplotype of ApoM gene may be a susceptible genotype of cerebral infarction.