AIM: To investigate the effect and safety of laser peripheral iridoplasty combined with iridectomy in the unmanageable acute angle - closure glaucoma by medication.
METHODS:Totally 19 cases (21 eyes) with acute angle-closure glaucoma, including 15 cases ( 17 eyes ) with primary glaucoma and 4 cases (4 eyes) with intumescent cataract - induced glaucoma, were recruited into the study. The intraocular pressure ( IOP ) of all cases were still >21mmHg after 24h drug treatment, and then were treated by laser peripheral iridoplasty combined with iridectomy. The visual accurity, IOP, cornea, peripheral anterior chamber depth, anterior chamber angle and complications were observed at 24h after the surgery.
RESULTS:The mean IOP of all cases was reduced from 53. 09±11. 01mmHg before the surgery to 14. 98±4. 21mmHg at 24h after the treatment, with significant statistical difference ( P< 0. 01 ). The visual acurity of all cases increased in different degrees from handle move to 0. 3 to 0-1-1. 0 at 24h after the treatment. In all cases, cornea edema reduced or cleared up, peripheral anterior chamber depth increased, and anterior chamber angle reopened in different degrees. Complications included iris hemorrhage in 11 eyes (52. 4%), mild iritis in 21 eyes (100%). No cornea burn was encountered.
CONCLUSION: Laser peripheral iridoplasty combined with iridectomy is an effective and safe method for the treatment of the unmanageable acute angle - closure glaucoma by medication.

Exposure to traffic-related air pollutants(TRAP)has been implicated in airway allergic diseases.Recent findings include epidemiologic and mechanistic studies that shed new light on the impact of TRAP on allergic rhinitis(AR)and the biology underlying this impact.These studies have found that oxidative stress induced by TRAP could affect the axis of epithelial cell-dendritic cell-T cell towards a T-helper 2 immune response,which is the major mechanism between TRAP and AR.Further,epigenetics and microRNA might be involved in this process.Our review will summarize the most recent findings in each of these areas.
Air Pollutants ; toxicity ; Air Pollution ; Humans ; Oxidative Stress ; Rhinitis, Allergic ; etiology ; Vehicle Emissions ; toxicity

The genes of P53,retinoblastoma (Rb),C-myc and human papilloma virus (HPV) were determined by PCR method in specimens of 33 patients with laryngeal carcinoma and 4 normal laryngeal tissues as a control group.There were 23 males and 10 females,ranging in age from 40 to 11 years old.Results showed that the mutation rate of P53 and Rb gene were 69.69%(23/33) and 48.48%(16/33),respectively;c\|myc gene amplification rate and HPV insertion rate were 58.62% (17/29) and 72.72%(24/33),respectively.There were significant difference(P＜0.05)in comparison between positive and negative expression above these genes.The expression rate were 50.00%(12/24),45.83%(11/24)and 66.67%(16/24)of C-myc,Rb and P53 genes respectively in 24 cases who were HPV positive of laryngeal carcinoma.The positive rates were 73.68%(14/19),63.16%(12/19),57.89%(11/19)and 84.21%(16/19) of HPV,C-myc,Rb and P53,respectively in 19 cases who have heavy smoking (20/day,20 years smoking history).Above results suggested that there were closed relationship in the C-myc gene activation and P53,Rb genes inactivation with smoking and HPV infection.It was believed that carcinogen factors on laryngeal carcinoma was the result of factors,genes and stages interaction.

The paper analyzed the necessity of the testing items for blood transfusion compatibility.By means of a comprehensive evaluation of the testing requirements of blood transfusion labs,and a basis on characteristics of blood transfusion profession and technical specifications in clinical transfusion,the paper probed into building appropriate lab examination techniques in terms of ABO blood type testing item,RhD blood type testing item and screening test,and cross-matching testing items,for the purpose of achieving the safety and effectiveness of clinical blood use.

Objective To investigate the protein and mRNA expression of NF-κB and zinc finger protein A20 in the keloid and normal skin and to explore its underlying molecular biological mechanism.Methods The protein and mRNA expression of NF-κB p65 and zinc finger protein A20 in keloid and normal skin was detected by immunohistochemistry and real time quantitative reverse transcription-polymerase chain reaction (RT-PCR).Results The protein and mRNA expression of NF-κB p65 in keloid tissues was higher than those in normal skin tissues (P＜0.05).The protein and mRNA expression of zinc finger protein A20 in keloid tissues was lower than those in normal skin tissues (P＜0.05).Conclusions NF-κB signal pathways and zinc finger protein A20 could play a role in keloid pathogenesis.

Objective To investigate the incidence,treatment and prognosis of neonatal respiratory failure (NRF) in the Neonatal Intensive Care Unit of Children's Hospital of Hebei Province.Methods The medical data of 626 NRF newborns admitted to the Neonatal Intensive Care Unit of Children's Hospital of Hebei Province from January 1st,2008 to December 31st,2008 were collected,and the pathogenesis of NRF,management,risk factors of their deaths and cost for the disease were studied.The results were compared with the national study in the same period and the local study of 2004-2005 by Chi-square test.Results During the 12 consecutive months,the incidence of NRF in neonatal intensive care unit was 38.9％ (626/1608).The mortality in hospital was 22.5％ (141/626),among which,most deaths (134,95.0％) occured after early discharge required by the parents.The mortality decreased by 14.7％ compared with that during 2004 2005 (37.2％,113/304),and was also lower than that (24.7％,1683/6864) in the national study at the same period.Pneumonia/sepsis ( 34.8 ％,218/626 ),neonatal respiratory distress syndrome ( 31.6 ％,198/626 ),and meconium aspiration syndrome (10.7％,67/626) were leading primary causes of NRF.The major complications were pneumonia/sepsis (5.4 ％,34/626),intracranial hemorrhage (4.6 ％,29/626) and persistent pulmonary hypertension (3.2％,20/626 ).The percentage of NRF babies requiring pulmonary surfactant rose from 14.1％ (43/304) during 2004-2005 to 23.6％ (149/626) in 2008,which almost reached the national level of 26.8％ (1840/6864).Pulmonary surfactant treatment was accepted by 70.2％ (139/198) patients with neonatal respiratory distress syndrome.12.1％ (24/198)of neonatal respiratory distress syndrome infant was administered immediate intubation-surfactant-extubation.Seventeen (2.7％) patients accepted nitric oxygen treatment.Nasal continuous positive airway pressure was given to 76.5％(479/626) patients,which was higher than before (47.1％,143/304).Conventional mechanical ventilation rate decreased from 72.7％ (221/304) to 49.8％ (312/626),and high frequency oscillatory rate increased from 0.7％ (2/304) to 10.5％ (66/626),which were similar to the national level.Of the 480 patients discharged after recovery,the mean time of hospital stay was (15.1 ± 4.0) days,and the mean medical cost was (12752 ± 5148) CNY.Conclusions The patient number increases and the medical skills and treatment methods of our hospital improve a lot.Utilization of pulmonary surfactant,nasal continuous positive airway pressure and high frequency oscillatory ventilation increase significantly.However,the burden of NRF is still heavy.

AIM: To establish an HPLC for determining notoginsenoside R1,ginsenoside Rg1,ginsenoside Rb1 in Xueshuantong Injection (total saponin of Radix et Rhizoma Notoginseng).METHODS: The analysis was performed on UltimateTM XB C18 column(4.6 mm ? 250 mm,5 ?m) with a gradient mobile phase of acetonitrile(A)water(B) (0-30 min,20% A→38% A;30-31 min 38% A→20% A;31-40 min,20% A) at a flow rate of 1.0 mL/min.The column temperature was maintained at 40 ℃,and the detection wavelength was set at 203 nm.RESULTS:The linear range of notoginsenoside R1 fell within 0.40 -4.01 ?g (r =0.999 9 ) and the average recovery was 99.36 % (RSD = 0.72% ,n = 6).The linear range of ginsenoside Rg1 fell within 1.47-14.69 ?g (r = 0.999 9) and the average recovery rate was 99.41% (RSD = 0.52% ,n = 6).The linear range of ginsenoside Rb1 fell within 1.50-15.03 ?g(r = 0.999 9) and the average recovery rate was 99.47% (RSD = 0.50% ,n = 6).CONCLUSION: This method is accurate,simple,rapid and can be used for the quality control of Xueshuantong Injection.

Most compounds in blood are blocked from flowing into brain by blood-brain barrier(BBB) to keep central nerve system normal, but this barrier will be open in different diseases with different mechanisms. As these mechanisms are understood more and more clearly, it will do help to protect blood-brain barrier and treat diseases which are correlative to blood-brain barrier. At the same time, it may also be helpful to let drugs entering central nerve system through BBB.

Objective Mapping the sites of earliest activation in AF patients with rheumatic heart disease. Finding out the ratio of AF originate from the pulmonary veins. Methods There were 9 patients with valvular atrial fibrillation (1 male, 8 females age (42?13) years, histories of rheumatic heart disease (11?9) years, and mitral valve area 1.01?0.02 cm2) involved in the research. 3 patients had organized thrombus in left atrial appendage. 6 patients with persistent AF received anticoagulation therapy (warfarin 2 or 3 weeks) and drug cardioversion (amiodarone 400 mg, three times per day, for 7 days) before procedures, while other 3 patients with paroxysmal AF received neither anticoagulation therapy nor drug cardioversion. All patients received percutaneous balloon mitral valvotomy (PBMV). After finished PBMV, four multipolar electrode catheters were placed in the high right atrium (HRA), coronary sinus (CS), left atrium (LA) and pulmonary veins (PVs). S1S2 and S1S2S3 programmed stimuli were delivered in HRA, CS, LA and PVs respectively. For the patients who failed to induce AF, burst stimuli were used. Results 11 AF generating sites, which induced by S1S2 and S1S2S3 programmed stimuli, could be confirmed by identification of the earliest regions of atrial activation for the first AF cycle. However, 1 AF obtained by 260 ms RR interval burst stimuli, affirmed by shortest activation cycle length. All confirmed 12 AF original sites were original as following: RA (n=4), LA (n=1), CS (n=2); PVs (n=5). Among the 5 PVs original sites, 3 was from left superior pulmonary vein, while the other 2 were from right superior pulmonary vein and left inferior pulmonary respectively. Conclusion PVs could be the ectopic origin of valvular AF.

Objective To investigate the effects of methylmercury on outward potassium currents in isolated outer hair cells of guinea pig cochlea.Methods The whole-cell patch clamp technique was used.Calcium sensitive outword potassium currents were recorded when the concentrations of mercurial were 1/1000 LD_(50),1/100 LD_(50) and 1/10 LD_(50).Results After methylmercury poisoning,amplitudes of the outword potassium currents I_(K(Ca)) were(1.48?0.28),(1.36?0.16) and(1.22?0.13)nA.Compared with control group,the amplitudes in three methylmercury poisoning groups were decreased by 8%,16% and 25%(P