Objective To investigate the imaging characteristics of primary abdominal cocoon in order to improve diagnosis level.Methods The imaging data of 5 cases of primary abdominal cocoon proved by surgery and pathology were retrospectively analyzed.Results Abdominal plain X-ray suggested intestinal obstruction in 3 out of 5 cases.The gastrointestinal barium meal showed cauliflower signal in all 5 cases;CT suggested a conglomeration of multiple small bowel loops in the 5 cases and the intestinal loops seemed to be encapsulated in a thickened capsule.Conclusion Abdominal cocoon should be taken into consideration when gastrointestinal barium meal shows cauli flower signal and CT displays conglomeration.

Objective To explore the diagnostic value of multislice spiral CT enterography(MSCTE) in patients with primary tumors of the small intestine.Methods Clinical data of 40 patients with primary tumors of the small intestine from Jan.2011 to Dec.2013 were analyzed.All patients were underwent MSCTE examination.Analyzed the coincidence rate between diagnostic results and pathological diagnosis.Results Pathological diagnosis of all patients showed that 15 cases were with small intestinal stromal tumors,10 cases with primary intestinal adenocarcinoma,9 cases with intestinal lymphoma and 6 cases with leiomyoma.The diagnoses of MSCTE were as follows:Twelve cases were with gastro intestinalstromaltumors,10 cases with primary intestinal adenocarcinoma,9 cases with intestinal lymphoma and 9 cases with leiomyoma.Consistency test(Kappa test) results showed that there was a good consistency between two dignosis methods(Kappa values =0.71).The coincidence rate of MSCTE in the diagnosis of primary tumors of the small intestine was 92.5％.Conclusion MSCTE is a convenient and safe method with high degree of accuracy,which provides a broad prospect for the diagnosis of primary tumors of the small intestine.

Interleukin-10 (IL-10) is an immuno-regulatory cytokine,which is well known for its immunosuppressive function.IL-1O can act on a variety of immune cell subsets and play an immunosuppressive effect through a variety of ways.It plays an important role in the tumor immune escape.Recent studies show that IL-10 also has an immune-stimulating function,which is presumably a pluripotent cytokine.This paper will combine the latest literature to summary the positive and negative regulatory roles of IL-10,mediation ways in the tumor and the relationships with prognosis.

Objective:To analyze the changes of chemical compositions and relative contents of volatile constituents in raw Xanthi-um sibiricum Patr. and fried Xanthium sibiricum Patr. Methods: Headspace solid phase micro extraction ( HS-SPME) combined with gas chromatography-mass spectrometry ( GC-MS) was used to study the volatile components in raw and fried products of Xanthium sibir-icum Patr. . A peak area normalization method was applied to calculate the relative percentage content of each composition. Results:Totally 26 and 27 kinds of compounds were isolated and identified respectively from raw and fried xanthium including 19 species and re-spectively accounting for 68. 69% and 90. 08% of the total volatile components. In the raw products, the components with high con-tents were camphor (15. 3%), eucalyptus oil alcohol (9. 6%) and borneol (6. 89%), while in the fried products, those were men-thol (24. 09%), borneol (18. 48%) and eucalyptol (7. 19%). Conclusion:The relative contents of components with effects on rhi-nitis in the fried products are obviously higher than those in the raw products. HS-SPME-GC-MS can be used to detect the volatile com-positions and their relative contents in the raw products and fried products of Xanthium sibiricum Patr. . From the changes of volatile components, theoretical basis can be laid for the components with certain efficacy in raw and fried Xanthium sibiricum Patr. , and scien-tific basis can be provided for their further study and comprehensive utilization.

Myasthenia gravis is an autoimmune disorder resulting from the production of antibodies against acetylcholine receptors leading to the destruction of the postsynaptic membrane at the neuromuscular junction.Myasthenic crisis is defined as any myasthenia gravis exacerbation necessitating mechanical ventila-tion or post-operation extubation delayed beyond 24 hours because of respiratory weakness.Most patients presenting with myasthenic crisis have an identifiable risk factor.The diagnosis of myasthenic crisis should be suspected in all patients with respiratory failure,particularly those with unclear etiology.Acute management of myasthenic crisis includes ventilatory therapy,removal of the offending trigger and immunomodulatory treat-ments.The latter includes plasma exchange or intravenous immunoglobulin,and high dose corticosteroids therapy.

Objective To study the mechanism of the effects of db cAMP on the inhibition of cell proliferation and phenotypic changes in transformed cells. Methods The progression of cell cycle was analyzed by the flow cytometry.The transforming phenotype of C 3H 10 T 1/2 cells was observed by the soft agar colony formation.Northern blotting and radioimmunoassay were used to analyze the level of CaM expression.The activity of PKⅡ was determined through ? 32 p ATP incorporation assay. Results The proliferation of transformed C 3H 10 T 1/2 cells was inhibited markedly by 1mmol/L db cAMP.The db cAMP treated transformed cells were accumulated at G 1 phase.After treatment with db cAMP,the soft agar colony formation efficient in transformed cells was decreased evidently,simultaneously,cells altered in morphology,from rounded to flattened shape.In contrast to normal cells,CaM level and PKⅡ activity increased in transformed cells.However,both CaM level and PKⅡ activity in transformed cells treated by db cAMP decreased obviously.Conclusion\ The results indicate that not only alteration of CaM expression,but also the changes of the PKⅡ activity are involved in account for the cell transformation and induced differentiation of transformed cells.It is possible that the role for CaM in the cell transformation and induced differentiation may be mediatied by the effect of PKⅡ. \;[

Objective: To study the quality standard of Fangtuoshengfaling Lotion (Radix et Rhizoma Rhei, Radix Sophorae Flavescentis, Radix Polygoni Multiflori, Radix Angelicae Sinensis, Radix Astragali seu Hedysarion, etc.). Methods: Radix et Rhizoma Rhei, Radix Sophorae Flavescentis were identified by TLC. The content of 2,3,5,4′ tetrahydroxystibene 2 O ? D glucoside in Radix Polygoni Multiflori of lotion was assayed by HPLC. Results: The linearity of 2,3,5,4′ tetrahydroxystibene 2 O ? D glucoside was found in the range from 0.36～1.84ng. The average recovery was 96.866% and RSD =0.898%( n =5). Conclusion: The method was found to be simple, accurate and with a good reproducibility and may be used for the quality control of the Lotion.

The investigation was conducted among eight untrained male and six well-trained male cyclists with the purpose of finding out the relationship between muscle fiber composition and lactate threshold. Muscle biopsy samples were taken of vastus lateralis to determine the composition of fiber type (Ⅰ, Ⅱ). Maximal oxygen uptake was measured in a progressive cycle ergometer test, while blood lactate threshold was determined on the basis of a systematic increase in BLa above baseline warm-up values. Analyses show that the percentage of type Ⅰ is closely correlated with lactate threshold in both groups of subjects and that muscle fiber composition plays (?) important role in determining lactate threshold. Therefore, an assessment of muscle fiber composition is useful for spotting athletes with high potential for endurance performance.

Acute kidney injury(AKI) is defined as functional and structural disorder or signs of renal damage including any defect from blood and urine test,or tissue imaging that is less than 3 months.The current treatment for AKI includes therapeutic agents and renal replacement therapy (RRT).The goal of RRT is to remove endogenous and exogenous toxins,inflammatory mediator,pathogenic factor,and to maintain fluid,electrolyte,and acid-base balance until renal function returns.The principal methods of RRT include intermittent hemodialysis,continuous renal replacement therapy,peritoneal dialysis,plasmaphresis exchange and hemoperfusion.In this article,we discussed the timing,choice of therapeutic mode,indication and common technical issues of RRT in pediatric AKI.

ObjectiveTo investigate the effect of bone marrow-derived mesenchymal stem cells (MSCs) on collagen-induced arthritis(CIA) and the possible mechanisms. MethodsMSCs from C57BL/6 mice bone marrow were isolated and expanded. Three DBA-I mice were as normal control group (Sodium Chloride injected on day 0 and 21) after primary immunization. After the bovine collagen Ⅱ induced-CIA models were established, fifteen male mice were randomly divided into 3 groups according to treatment regi-mens: CIA control group(0.2 ml Sodium Chloride injected through caudal vein on day 0 and 21), prevention group(1×10+6 cells MSC on day 0) and treatment group. (1×10+6 cells MSC on day 21 after primary immun-ization). The observed parameters included arthritis index (Al), articular pathology changes, levels of serum TNF-α, IL-1β detected hy ELISA. In addition, the percentage of CD4+CD25+Foxp3+Treg cells(Tregs) in CD4+ T cells of spleen or lymphoid node from mice were also analyzed by flow cytometry(FCM). Results①The Al and articular pathological score of the prevention group and treatment group were lower than the CIA control group(P<0.05). ②The levels of serum TNF-α and IL-1β of normal control, prevention group and treatment group were significantly decreased compared with CIA control group(P<0.05), and positively correlated with Al or articular pathological score (P<0.05). ③The percentage of Tregs from spleen and lymph nodes was significantly increased in MSCs treated mice(P<0.05). Conclusion MSCs transplantation is effective for CIA and suppression of TNF-α, IL-1β and up-regulation of Tregs by MSCs may be the possible mechanism in CIA.