Objective:To develop a new nucleic amplication method for detection of Chlamydia trachomatis DNA by gap ligase chain reaction(G-LCR).Methods:A G-LCR DNA amplification assay that targeted the outer major membrane protein gene(omp1)of CT was established to detect CT infection.The sensitivity and specificity of a newly developed G-LCR test was examined by the use of highly purified elementary bodies (EBs).DNA fragments of different species and from other bacteria1 were detected with G-LCR and routine polymerase chain reaction (PCR).Results:Using G-LCR,DNA fragments of 54bp were amplified from five different species.The sensitivity could be improved to detect out 2 chlamydial elementary bodies.G-LCR detected ten-fold EBs than PCR.No signal was observed when C.pneumoniae and other bacteria were used as templates.Conclusion:G-LCR is sensitive,rapid and specific for detection of Chlamydia trachomatis.

In view of the fact that the Pharmaceutical Patent Law will be put into effect in China,the authors suggest that the development of new drugs in China should be relied mainly rather on creating than on copying. However,some of the effective drugs should be still modelled on foreign products. We should pay great attention to the research work on pharmaceutical preparations and the development of Chinese patent medicine, intermediates of preparation and reagents.

It is generally agreed that motivation is one of the most important factors influencing the students success or failure in learning a language. Motivation, according to Harmer, is an internal drive that encourages the learner to pursue a course of action(1983: 3). Expressed more colloquially, when students perceive an attractive goal that they wish to achieve, they may do whatever is necessary to reach it. Then they are strongly motivated.This article aims at introducing the classification teaching method into English teaching. Studies suggest that classification teaching method has positive effects on motivation.

Fatal Outcome ; Humans ; Infant, Newborn ; Kidney ; abnormalities ; Male ; Pneumothorax ; etiology ; Respiratory Distress Syndrome, Newborn ; etiology ; Syndrome

Objective:To explore the relations between freshmen's self-identity status,personality characteristics and mental health status.Methods:188 new students were asked to complete the questionnaires of mental health scale and personality scale from the China college students mental health evaluation system,and eollnge students self- identity status scale.Results:①the most status of identity is achievement(43.1%),then the identity diffusion (30.9%);②male students in compare with female,were more likely in achievement status(60.7?13.8/53.9?11.4,P0.406);③the achieve- ment status had obvious negative correlation with depression,dependency(r=-0.17～-0.24),the diffusion status had obvious positive correlation with depression,social-withdrawal,and impulsion(r=0.15～0.34);④active,ac- commodating,tough-minded personality characteristics had obviously negative correlation with mental symptoms(r= -0.16～-0.56).Conclusions:encourage college students to form the achievement ego-identity status,to be ac- tive,accommodating and tough-minded,will be helpful for their psychological health.

Mammalian target of rapamycin (mTOR)is an atypical serine/threonine protein kinase,which is a key protein kinase regulating cell growth,proliferation,translation,metabolism and autophagy.It's first known beca- nuse inhibited by rapamycin.Research indicated that mTOR plays a key regulating role in physiological and patho- logical process of cardiovascular system.The present article mainly reviewed research progress of mTOR signal path- way effect in cardiovascular system.

Objective To investigate the positive and suspicious rates of BV, SRV, SIV and STLV-1 antibody of the cynomolgus monkeys introduced from Laos, dynamic monitoring of virus antibody during the establishment of SPF cyno-molgus monkey population, and then comparing the positive rate of virus antibody in the baby monkeys between the common with SPF populations.Methods Continuous monitoring four kinds of virus in the monkeys was carried out using special reagent kits and analyzed the data.Results In the introduced cynomolgus monkeys (n=1998), the positive rate of BV antibody was 52.35%, suspicious rate of BV antibody was 8.31%, and negative rate of BV antibody was only 39.34%, the positive rates of SRV and STLV-1 antibody were 7.45%and 8.56%, respectively, and positive or suspicious SIV anti-body in the cynomolgus monkeys was not detected.After screening and establishment of the SPF population, the positive rates of BV, SRV and STLV-1 antibody were 5.24%, 1.01%and 0.4%, respectively, in the year 2010.Then after five years of screening and elimination, by the end of 2014, the positive rates of BV, SRV and STLV-1 antibodies were 0.82%, 0.27%and 0.27%, respectively, and positive or suspicious SIV antibody in the cynomolgus monkeys was not de-tected.The BV antibody positive rate of the baby monkeys was 9.71%and the suspicious rate was 1.85%in the common population, but only 0.22%of the baby monkeys in the SPF population.Conclusions Continuous monitoring virus anti-bodies and eliminating positive and suspicious animals have important significance in the establishment of a SPF cynomolgus monkey population.

Objective:To optimize the extraction process of Guilong cataplasm .Methods:The yield of volatile oil was used as the index to optimize the volatile oil extraction technology by single factor tests. The yield of dry extract and the content of aconitine were used as the indices to optimize the water extraction technology by single factor tests and orthogonal test .Results:The optimal extraction conditions of volatile oil were as follows:the soaking time was 1h with 8-fold volume of water , and the extraction time was 6 hours.The optimal water extraction conditions were as follows:using 8-fold volume of water extracted for 3 times with 1 h for each time .Conclu-sion:The extraction process is simple , reasonable and reproducible ,which is suitable for the next research of Guilong cataplasm .

Objective To study the genetic polymorphism of Nanjiang yellow goat and to investigate the feasibility of this research by AFLP. Methods The genomic DNAs of 15 goats were analyzed with RAPD and AFLP molecule marking techniques. The effects of the two methods were compared. Results The rates of the polymorphic loci and the genetic similar index of population by RAPD marker were 26.98% and 0.942 respectively, and that by 31.4% AFLP and 0.874 respectively. Conclusion AFLP and RAPD technique are effective methods in genetic polymorphism monitoring of Nanjiang yellow goats, and supply useful parameter with appraise genetic stability.

Objective:To investigate the preventive effect of nalmefene on sufentanil-induced cough in the patients with general anesthesia. Methods:Eighty patients with general anesthesia were randomly divided into the control group and the nalmefene group. The nalmefene group was intravenously given 0. 25μg·kg-1 hydrochloric acid natrium nalmefene 5 minutes before the induction, and physiological saline with the same capacity was given in the control group. Cough number and intensity in one minute after the injection of nalmefene were observed, and the changes of hemodynamic indices such as the blood pressure, heart rate and pulse oxygen satura-tion before the anesthesia induction (T0), 1min after sufentanil injection (T1) and after the intubation (T2) were observed and com-pared between the groups. Results:The incidence rate of cough was 37. 5% in the control group and 0% in the nalmefene group, and the incidence rate and strength of cough in nalmefene group were both lower than those in the control group (P<0. 05). The hemody-namic parameters at T1 showed notable changes when compared with those at T0 in the control group (P<0. 05), and had significant differences when compared with those in the nalmefene group (P<0. 05), and at T2, all the parameters recovered to the levels at T0. The parameters in the nalmefene group were much more steady than those in the control group (P>0. 05). Conclusion:Pretreatment with hydrochloric acid natrium nalmefene can prevent sufentanil-induced cough response during the induction of anesthesia without weakening the inhibitory effect of sufentanil on intubation response.