In order to explore the cellular mechanism of antishock effect of anisodamine (654-2), the changes of intramitochondrial magnesium during late hemorrhagic shock were observed. Results showed that there exists a negative correlation between the blood plasma magnesium concentration and arterial blood pressure in shocked animals and at the same time the magneium content of liver mitochondria reduced significantly; 654-2 prevented the decrease of mitochondria magnesium and the increase of blood plasma magnesium, and improved the prognosis of the shocked animals. These results indicate that 654-2 may protect the liver mitochondria of shocked animals at a cellular and subcellular levels.

Hand, foot, and mouth disease (HFMD) is a viral infectious disease regarded to be a public-health problem worldwide. Since the 1990s, HFMD began to spread in the Asia-Pacific region (especially in South-East Asia). HFMD outbreaks have occurred in mainland China frequently since 2008, and the morbidity and mortality of HFMD has continued to increase in recent years. In mainland China, enterovirus A serotype enterovirus A71 (EV-A71) and coxsackievirus A16 (CVA16) have been the major pathogens of HFMD during these years. However, the etiological spectrum of HFMD changes with time. This review focuses mainly on the etiological spectrum of HFMD and changes in epidemic patterns in mainland China.
China ; epidemiology ; Disease Outbreaks ; Enterovirus ; classification ; genetics ; isolation & purification ; Hand, Foot and Mouth Disease ; epidemiology ; virology ; Humans ; Prevalence

Objective To study the clinical effects of femtosecond laser surgery and phacoemulsification in patients with cataract. Methods A total of 80 patients were randomly divided into study group and control group. 40 patients with 44 cataract eyes in control group were treated by routine phacoemulsification and 40 patients with 47 cataract eyes in control group were treated by femtosecond laser additionally. The capsulotomy diameter, capsular tension ring, capsule size and operation time difference were compared between two groups. The vision and Intraocular lens decentration rate were compared between two groups. Results The capsulotomy diameter in study group was significantly less than control group (P < 0.01). The apsular tension ring in study group was significantly more than control group(P < 0.05). The capsule size in study group was significantly less than control group (P < 0.01). The operation time in study group was significantly less than control group (P >0.05). The vision in study group was significantly better than those in control group during a year (P < 0.05) and the adverse reaction rate was significantly less (P < 0.05). Conclusion Femtosecond laser combined with phacoemulsification has a great clinical effects which improve patients′ vision and reduce adverse reaction rate makes it worth for clinical application.

Objective To observe the effects of Donepezil on cognitive function of delyed ephalophthy after acute carbon monoxide poisoning ( DEACMP) .Methods Eighty patients with DEACMP were randomly divided into control group ( 40 cases ) and treatment group ( 40 cases ) .The control group was given treatment of regular medicine, placebo and hyperbaric oxygenation.The treatment group was given treatment of regular medicine, Donepezil and hyperbaric oxygenation.The dose of Donepezil was 5.0 mg per night, and 90 d was a period of treatment.The cognitive function of patients was evaluated respectively by MMSE and Barthel Index rating scale ( BI) .And the results were compared between the two groups.Results Compared with before treatment, the MMSE and BI scores of the two groups after treatment were significantly increased (P<0.05 -0.01).And after the treatment, the MMSE and BI scores of the treatment group were significantly higher than those in the control group (P<0.05-0.01).Conclusion Donepezil can greatly improve the cognitive function of DEACMP patients, it is worthy to be clinical promoted.

The most important characteristic of optical coherence tomography (OCT)is high resolution (about 10~15μm),so it can perform high resolution cross section tomography on internal microstructure of biological tissue. From 2000 OCT first used to detect coronary artery lesions,it already gradually popularized in diagnosis and treat-ment field of coronary artery intervention,It has become a main method for intravascular detection,diagnosis and guiding treatment of coronary diseases.

Purpose CT spectroscopy imaging was used in the preoperative differentiation evaluation of renal clear cell carcinoma, to access its malignant degree preoperatively, and to guide the operation treatment. Materials and Methods The spectral characteristics of 40 patients with renal clear cell carcinoma (RCCC) were analyzed retrospectively, all the RCCC patients underwent gemstone spectral imaging (GSI) scans, to obtain spectral serial images for the arterial phase and medulla phase. Spectral characteristic parameters and spectrum curve between different grades of renal cell carcinoma was compared, and the results were compared with pathology. Results Among the 40 cases of patients, carcinoma of grade I, II and III were 13 cases, 15 cases and 12 cases respectively. CT value ratio of renal clear cell carcinoma of grade I, II and III under 70 keV were 1.17±0.25, 0.84±0.85 and 0.64±0.19 (F=23.697, P<0.05) in arterial phase and 0.83±0.12, 0.64±0.07 and 0.54±0.08 (F=30.975, P<0.05) in medulla phase;iodine concentration ratio was 1.19±0.40, 0.60±0.10 and 0.25±0.94 (F=32.932, P<0.05) in arterial phase, and 0.69±0.18, 0.43±0.94 and 0.26±0.51 (F=28.673, P<0.05) in medulla phase. Spectrum curve slope of renal clear cell carcinoma of grade I, II, III in arterial phase was 4.00±1.24, 2.16±0.95 and 0.92±0.64 (F=23.392, P<0.05), and 3.54±1.20, 2.70±0.71 and 1.20±0.44 (F=24.272, P<0.05) in medulla phase. Differences of water concentration in arterial and medulla phase between renal clear cell carcinoma with different grade were not statistically signiifcant (P>0.05). Conclusion The differences of CT value, energy spectrum curve slope, iodine value under CT spectroscopy single energy imaging between renal clear cell carcinoma with different nuclear grade were statistically signiifcant, which can be expected to provide the basis for preoperative therapy selection.

Objective To evaluate the clinical value and safety of radiotherapy combined with tyrosine kinase inhibitors (TKI) in non-small cell lung cancer (NSCLC) with brain metastasis driven by epidermal growth factor receptor (EGFR) mutation.Methods A retrospective cohort of 21 non-small cell lung cancer (NSCLC) patients with EGFR mutation-driven brain metastasis was recruited.10 patients (treatment group) were randomly selected for radiotherapy combined with TKI treatment,and 11 patients (control group) were treated with radiotherapy.Tumor size was measured and clinical efficacy was evaluated by MRI in the four weeks after radiotherapy.Since then,a clinical response was evaluated every three months until disease progression,and the median overall survival time was calculated.Results The objective remission was 8 cases in treatment group and 4 cases in control group.The disease control was 9 cases in treatment group and 8 cases in control group.The median overall survival time of treatment and control groups was 11.2 months and 6.4 months,respectively.Adverse reactions in treatment group mainly contained nausea,diarrhea and rash,which all were tolerated.Conclusion Radiotherapy combined with TKI is superior to radiotherapy alone in effect and safety for treatment of NSCLC with EGFR mutation-driven brain metastasis.

Objective: To investigate the efficacy of acupuncture and point injection in cooperation with Tuina and reposition for treating lumbar intervertebral disc protrusion.Methods: Treatment was given by first acupuncture, then point injection and last Tuina and reposition. It was administered once daily, 6 times as a course and compared with simple Tuina and reposition. Results: Both the effective rate in the clinical treatment and the cure rate in the same period were significantly higher in the treatment group than in the control group. Conclusion: A composite treatment by acupuncture and point injection in cooperation with Tuina and reposition is better in curative effect and shorter in the course of treatment than simple Tuina and reposition.

Tension-free hernioptasty is a major method for treating inguinal hernia, characterizing by less wound, tension-free incision, sample processing, less dissection and separation, mild pain, rapid recovery, and short length of stay. In particular, transabdominal preperitoneal inguinal hernioplasty is considered as the most ideal method at physiologicoanatomical level. However, the effect of patch materials on postoperative recovery is generally ignored for a long time. Recent studies demonstrate that patch weight and mesh diameter are important factors for abdominal wall repairing and reconstruction. Polypropylene patch, characterizing by strong stability, high intensity, great unreactiveness, and sample processing, has become a major material for hernioptasty. However, there are still some problems of clinical application, including that polypropylene patch may induce intensive foreign body reaction and chronic inflammation as well as compliance decreasing of abdominal wall. Therefore, polypropylene patch should be chosen according to patient requirement, i.e., light, soft, and wide-aperture patch, so as to reduce onset of complication. Nerve and ligament tissues could be clearly observed from light and soft patch, and this might and prevent hurt by mistake, improve repairing operability, enhance correct putting of patch, and decrease incidence of pain. Furthermore, the structure was beneficial for in-growth of fiber tissue, longitudinal and transversal movement induced by body position alternation and muscle contraction, and reduction of discomfortableness.

Objective To investigate the characteristics and diagnostic value of fundus fluorescein angiography(FFA)for familial exudative vitreoretinopathy(FEVR).Methods 34 children(68 eyes)with FEVR and 64 parents(1 28 eyes)were included.All the clients were received examinations of slit-lamp biomicroscopy and indirect ophthalmoscopy.Meanwhile the children were examined by Retcam Ⅱ,the best corrected visual acuity of parents were recorded.The children and their parents were classified according to the ocular findings.Among 68 eyes of children,3 eyes(4.41% )were normal,4 eyes(5.88% )were in stage 1,7 eyes(10.29% )were in stage 2,2 eyes(2.94% )were in stage 3,8 eyes(11.76% )were in stage 4 and 44 eyes(64.71% )were in stage 5.Among 128 eyes of parents,74 eyes(57.81% )were normal,51eyes(39.84% )were in stage 1,1 eyes(O.78% )were in stage 2 and 2 eyes(1.56% )were in stage 5.FFA was performed on the children with RetcamⅡunder anesthesia and on the parents with HR2 in order to observe the FFA characteristics in different stage.Results FFA characte ristics in children included uncompleted vascularization of the periphery,peripheral avascular zone(stage 1);neovascularization and/or peripheral subretinal and intraretinal exudation(stage 2);subtotal retinal detachment with attached foyea (stage 3);subtotal retinal detachment with detached foyea(stage 4)and total retinal detachment(stage 5).FFA characteristics in parents included abrupt cessation of the peripheral retinal capillary network and a peripheral avascular zone(stage 1); abnormal peripheral arteriovenous shunts, neovascularization or exudation(stage 2)and atrophia bulbi(stage 5).Conclusions FEVR in different stage has different FFA characteristics.FFA plays an important role in early diagnosis of FEVR.