Defensins, a family of 3-4 KD cationic peptides, have been found recently in the cytoplasmicgranules of human, rabbit, guinea pig and rat polymorphonuclear neutrophils and rabbit alveolarmacrophages. The defence roles of these molecules, such as antimicrobial activity, viral inactiva-tion, tumor cell cytotoxicity, chemotactic and opsonic actions, etc. have been adequately studied.In order to investgate the role of Defensins in the neutrphil-mediated pathological process ofsome diseases, we isolated and purified human neutrophil Defensins. By using Sephadex G100and Bio-gel P10 gel filtration chromatography, the purified molecules were obtained. The puritywas identified by AU-PAGE,SDS-PAGE, and amino acid composition analysis. In vitro fugicidalactivity of the purified human neutrophil Defensins against Candida albicans was also examined.When incubated with 33 Mol concentration of human Defensins, more than 90% of Candida,al-bicans were killed in the methylene blue assay system.

Antirheumatic Agents ; therapeutic use ; Arthritis, Rheumatoid ; drug therapy ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Etanercept ; Female ; Humans ; Immunoglobulin G ; therapeutic use ; Male ; Methotrexate ; therapeutic use ; Phytotherapy ; Prednisone ; therapeutic use ; Receptors, Tumor Necrosis Factor ; therapeutic use

Notch signal pathway is closely related to the organism's development and a variety of cancers. Current models available for screening modulators of Notch signal pathway all use mouse Notch protein as substrates and those models which use human Notch protein have not been reported. To make the screen results much more reliable, the authors cloned a truncated form of human Notch1 called N100, and built the screening models for the use of it instead of mouse Notch protein. The models included an in vitro screening model based on the purified gamma-secretase enzyme and a cell model using luciferase reporter system. The screening models then have been verified by the known modulators of Notch signal pathway and the IC50 values have been obtained. The verified models can be used to screen modulators of human Notch signaling pathway effectively and it can lay the foundation for finding new modulators of this kind effectively.
Amyloid Precursor Protein Secretases ; Animals ; Drug Evaluation, Preclinical ; methods ; Humans ; Luciferases ; Mice ; Receptors, Notch ; antagonists & inhibitors ; Signal Transduction

[Objective] This paper sums up professor Qi Xianghua's five psychological disorders of TCM scholar thought and clinical experience in diagnos-ing and treating insomnia. [Methods] By the pulse diagnosis, discern the five kings of psychological disorders, including irritable, anxiety, palpitation due to fright, depressed, and an medical record of professor Qi Xianghua is cited.[Results] The syndrome differentiation should be based on the five kings of psy-chological disorders.[Conclusions] Professor Qi Xianghua's five psychological disorders of TCM on treating insomnia are worth to extend and apply.

In recent years, with the further study of cell biology and genetics, the role of autophagy in bronchial asthma is becoming more and more obvious. Especially in the aspects of genetic factors, oxidative stress and environment, autophagy regulation may directly affect the occurrence and development of asthma, which may become a new direction of asthma treat-ment. In this review we will discuss what is autophagy, and the relationship between autophagy and asthma.

Objective To investigate the characteristics of the clinic manifestations and the electromyogram changes in patients with chronic N2hexane intoxication. Methods The clinic manifestations, the electromyography and nerve conduction velocities were analyzed in 9 patients with N2hexane intoxi2 cation. Results The main clinical manifestations of the disease were acroparesthesia and asthenia, especially in legs. The abnormality rate of electromyography in patients with N2hexane intoxication was 100%. The conduction velocity of the sensory nerve and the motor nerve decreased at a rate of 33.33% and 88.89% respectively. The distal latencies were prolonged( 100% ). The denervated electromyogram was 33.33%. Conclusion The main clinical manifestations of the disease were Peripheral neuropathy. The chronic N2hexane intoxication mainly led to the imparement, especially in the distal region of the motor nerve in legs,and the damages of the nerve could be related to the duration of exposure to N2hexane.

OBJECTIVE:To establish an HPLC method for determining the content of main components in minocycline hydrochloride sustained-release tablets.METHODS:The sample was separated on ODS-C_(18)column with the mobile phase consisted of 0.2 mol?L~(-1)ammonium oxalate-0.01 mol?L~(-1)edetate disodium-dimethylformamide-tetrahydrofuran (600:180:120:80)at a flow rate of 1.5 mL?min~(-1).The detection wavelength was set at 280 nm and the sample volume was 20?L.RESULTS:The linear range of minocycline was 0.05～0.5 mg?mL~(-1)(r=0.999 8)and the average recovery was 99.11%,RSD=0.5%.CONCLUSIONS:This method was proved to be accurate and reliable,sensitive and reproducible,and it can be used for determining the content of this tablets.

OBJECTIVE:To observe the clinical efficacy and safety of Shuanghuanglian oral liquid combined with cefuroxime axetil in the treatment of bacterial respiratory tract infection. METHODS:184 patients with bacterial respiratory tract infection were randomly divided into test group and control group. Test group was orally given 250 mg Cefuroxime axetil tablet,twice a day+20 ml Shuanghuanglian oral liquid,3 times a day. Control group was only given Cefuroxime axetil tablet (the same dosage as test group). The treatment course for 2 groups was 2 weeks. The cough duration,body temperature recovery time and runny nose disap-pearing time before and after treatment and bacterial clearance rate in 2 groups were observed,clinical efficacy and incidence of ad-verse reactions were recorded. RESULTS:After treatment,the total effective rate in test group was significantly higher than control group,cough duration,body temperature recovery time and runny nose disappearing time were significantly shorter than control group,the differences were statistically significant(P<0.05);and there were no significant differences in the bacterial clearance rate and incidence of adverse reactions(P>0.05). CONCLUSIONS:The efficacy of Shuanghuanglian oral liquid combined with ce-furoxime axetil is superior to cefuroxime axetil alone in the treatment of bacterial respiratory infections,with similar safety.

Objective To explore effect of clinical pathway in turnout gene therapy's health education,to promote turnout sufferer's acknowledge of gene therapy,thus to better cooperate the treatment.Methods To apply the clinical pathway to the in-patient health education,to explain and evaluate to the turnout gene therapy's sufferer.Resuits The sufferers'acknowledge of tumour gene therapy promoted and their cognition of the therapy's side effect promoted,so they can better regard gene therapy.Simultaneously the relationship of nurse-sufferer improve and the nurse's ability of health education improved.Conclusion Clinical pathway has good using value in the health educadon of promoting turnout gene therapy's sufferer's acknowledge.

BACKGROUND: The filling compactness can not be guaranteed due to apparatus retention in traditional lateral condensation.The warm gutter filling can achieve good results because of the easy changeable of gutta-percha in softening states.OBJECTIVE: To compare the warm gutta-percha fillings (Obtura Ⅱ and BeeFill 2 in1 filling) and cold lateral compacted gutta-percha with the bypassing root canal preparation in the intracanal separated instrument of oval canal.METHODS: Firstlythe 60 oval root canals of fiat mandibular central incisors were randomly divided into three groups, with 20 teeth in each group. After that, the root canal was filled with BeeFill 2 in1, Obtura Ⅱ condensation, and lateral condensation.The percentage of void at 5-mm to root tip versus cress section area was observed under a scanning electron microscope.RESULTS AND CONCLUSION: Under in vitro condition, the difference of percentage of void area versus root canal area between the Obtura Ⅱ condensation group and the lateral condensation group had significance (P=0.013), and the similar difference could be found between the BeeFill 2 in1 condensation group and the lateral condensation group (P=0.014), but the difference had no significance between the BeeFill 2 in1 condensation group and the Obtura Ⅱ condensation group (P=0.088).The results revealed that the root canal can be sealed accurately when establish hypass root next to the separated instrument and filled with BeeFill 2 in1 warm gutter filling.