Objective:To investigate the expressions of SEDL(X-linked spondyloepiphyseal dysplasiatarda,SEDL)gene and its encoded protein sedlin in a variety of tissues in mice.Methods:Real-Time PCR and Western Blot were used to detect the expression levels of the SEDL mRNA and sedlin protein in 9 tissues in mice,respectively.And the expression levels of them in bone tissues in mice of 1,2 and 4-week-old were also measured in the same way.Results:①Expressions of SEDL gene and its corresponding sedlin protein were existed in 9 tissues detected in mice.The relative expression levels of SEDL mRNA and sedlin protein in tissues in mice were significantly different (P

Objective To explore the clinical effect and application value of early acupressure combined with hot belly(hot compress on abdomen)to promote the recovery of gastrointestinal function after abdominal surgery. Methods A prospective random controlled study was conducted from January 2012 to October 2012,200 hospitalized patients having undergone abdominal surgery were randomly divided into a control group(100 cases)and an observation group(100 cases). Traditional nursing method was applied to take care of the abdominal postoperative patients in the control group,that was at post-operative 6 hours the patient may begin to turn over in bed,once 2 hours,and on the post-operative 1 day,according to the patient's disease situation,he or she may be allowed to ambulate in the room without any other additional measures,waiting for the recovery of gastrointestinal function. In the observation group, beside the traditional nursing method used in the control group,additionally,acupressure and abdominal fomentation was applied twice a day for 4 days. The postoperative bowel sound recovery time,the anus spontaneous exhaust time, the incidences of abdominal distension and intestinal obstruction were observed. Results In the observation group, the gurgling sound recovery time and anus spontaneous exhaust time were significantly shorter than those in the control group〔gurgling sound recovery time(hour):17.77±2.67 vs. 18.87±3.18,anus spontaneous exhaust time(hour):28.27±6.30 vs. 30.85±6.74,both P<0.01〕;the incidences of postoperative abdominal distention and intestinal obstruction were obviously lower than those in the control group(bloating:9.0%vs. 23.0%,intestinal obstruction:1.0% vs. 9.0%,both P<0.01). Conclusion Early application of acupressure combined with hot belly for abdominal postoperative patients can significantly promote the recovery of gastrointestinal function,that is beneficial to the reduction of the incidences of complications.

Objective To observe the infecion status and drug resistant rate of mycoplasma in infertility patients in Puyang city and to guide useing drug rationally.Methods A total of 500 women in reproductive period in Puyang city were recruited in this study,collected their vaginal discharge specimen,then cultured and detected ureaplasma urealyticum(Uu) and mycoplasma hominis(Mh) by liquid culture method and solid culture method.The women with positive results were divided as the pregnancy group and infertility group.The sensitivity rate of mycoplasma to 12 kinds of antibiotic were tested.Results The positive rate of mycoplasma in all the 500 women was 44.0%(220/500),the constituent ratio of pregnancy group(60 cases) was 27.3%,and the constituent ratio of infertility group (160 cases) was 72.7%.The difference was statistically significant between infertility group and pregnancy group(P<0.05).The Uu infection rates in pregnancy group and infertility group were 71.6% and 76.2% respectively,and the Mh infection rates were 11.6%,11.2% respectively.The rates of infecting Uu and Mh at the same time were 16.6% and 12.5%.The mycoplasma in and infertility group the pregnancy group were sensitive to spectinomycin,doxycycline and capminocim,which were 91.8%,92.1%,88.3%;90.9%,90.9%,87.3%.Conclusion The infection rate of Uu is higher than Mh in patients with infertility,which is resistant to quinolone classes and large ring lactone class antibiotic.

Objective To investigate the clinical and skeletal muscular pathological features of chronic progressive external ophthalmoplegia(CPEO).Methods The clinical and skeletal muscular pathological data of 6 patients with CPEO were analyzed retrospectively.Results All of the 6 patients onset were 4 to 18 years old.The first symptom was blepharoptosis.The clinical mainly manifestations were limited activity of eyeball,accompanied with limbs muscle fatigue,weakness and level elevatation of serum creatine kinase(CK).5 cases had myogenic changes in electromyography(EMG).The skeletal muscular pathological examination showed that the ragged red fibers(RRF)were scattered among the muscle fibers,and the deficient or absent of COX activity in many fibers.A few degenerating and necrotic fibers were observed in 4 cases.Lipid-drops were increased by oil red "O" stain in 3 cases.Electromicroscope showed that abnormal mitochondria increased in number and aggregated under sarcolemma or in cytoplasm.Crystalloid inclusion body could be observed.Conclusions The clinical mainly features of CPEO are external ophthalmoplegia and accompanied with limbs weakness.RRF and COX activity deficiency or absence according to skeletal muscle pathology can be suggest and support the diagnosis of CPEO.Gene sequencing based on skeletal muscle biopsy is necessary to make the final diagnosis of CPEO.

Functional dyspepsia is a common disease in clinical service.Its etiology and pathogenesis are related to a variety of factors,and among them,gastric motility and gastrointestinal motility dysfunction are major causes.At present,diagnosis of functional dyspepsia depends maindy on the complaint of patients and no gastric motility detection method or means are effective enough to complete the diagnosis objectively.The electrical bioimpedance technique extracts information of gastric electrical activity and mechanical movement base on the recngnition of electricity-mechanism compound system and studies complicated procedure of gastric electrical activity,mechanical contraction and gastric peristalsis.The electrical bio-impedance technique has shown a promising clinical application and development prospects.

OBJECTIVE:To evaluate the cost-effectiveness of3Chinese and western medicines in treating senile func?tional constipation.METHODS:186patients with senile functional constipation were randomly assigned to receive Forlax powder preparation for infusion,Liuweianxiao capsule and Buzhongyiqitang respectively,the therapeutic efficacies of the3groups were observed and the cost-effectiveness analysis were conducted12weeks after treatment.RESULTS:The cure rates for the3groups were77.6%,83.3%and85.5%,respectively;the costs for the3groups were963.52yuan,877.00yuan and850.12yuan,respectively;the ratio of cost to effectiveness were1241,1052and975,respectively;the incremental cost-effectiveness ratio of Group A and B were1435and1221as compared with Group C.CONCLUSION:Liuweianxiao capsule(Group B)was the preferred option for the treatment of senile functional constipation.

Objective To investigate the expression of COX-2 in multiple myeloma(MM)and the relationship between myeloma cells proliferation and apoptosis.To provide a new prognosis factor and therapeutic target.Methods COX-2 from the 22 newly diagnosed MM,14 relapsed MM and PCNA,HSP70 of the newly diagnosed patients were detected by immunohistochemistry method.Results All the newly diagnosed MM exhibited positive COX-2 immunoreactivity.50% had strong COX-2 and 50% showed weak COX-2.Relapsed MM exhibited strong COX-2.COX-2 was related with serum β2 microglobulin,marrow plasma cells,hemoglobin,PCNA,HSP70(P=0.019,0.003,0.048,0.006,0.034).Conclusion COX-2 was overexpressed in MM.Prognosis of patients with strong COX-2 is poorer than those with weak COX-2.COX-2 may promote the proliferation and inhibit the apoptosis of myeloma cells.

Objective To verify the effect of activin A neutralizing antibody (AANA) on inhibition of hepatic fibrosis and mRNA expression of various cytokines. Methods Mouse hepatic fibrosis model was induced by CCl_4. Male Kun-ming mice were divided into 5 groups: normal control group,olive oil control group, CCl_4 model group, AANA group and antibody control group. The mRNA expressions of activin A, transfer growth factor (TGF)-?_1, platelet derived growth factor (PDGF) and tissue inhibitors of metalloproteinas (TIMP)-1 in liver tissue were determined by semiquantitative reverse transcription-polymerase chain reaction(RT-PCR) analysis. Results Compared with CCl_4 model group and antibody control group, the pathologic changes of hepatic fibrosis were ameliorated in AANA group, the mRNA expressions of activin A,TGF-?_1, PDGF and TIMP-1 were down-regulated in AANA group ( P

Objective:To develop an economical,simple and portable device for imitation of ship shock motion produced by non-contacting under water explosion.Methods:The device was developed based on aerodynamical principles and sensor detection technology,and was tested with animal experiments to verify its performance.The injuries caused by 4 grades of shock motions with different accelerations,duration and displacement were observed in 40 black rabbits(10 for each grade).Results:The device was safe and functionally stable,with a peak acceleration of 1 000 m/s~2,a shock duration of about 2 ms and a device displacement of 100 mm.The accelerations of 4 shock motion grades were significantly different and with good reproducibility.The damage to the rabbits were mainly haemorrhage of different extents in organs of pleuroperitoneal cavity,especially in the lungs,but no rupture of organs was found.The degree and involvement of damage had an increasing tendency with the increase of acceleration of shock motion.Conclusion:The device we developed can imitate the effects of ship shock motion.

To investigate the alterations of contractile function of VSM and it's primary mechanism in hemorrhagic shock rats, contractile tension of vascular rings induced seperately by NE,PE,Caffeine and K + was measured with tension conductor and physiological recorder. The results showed that after 2h and 4h of shock, contractile tension of vascular rings to NE was 76 17% and 66 50% as compared to the control group; contractile tension to 20 mmol/L caffeine and over 20 mmol/L K + was significantly decreased after 2h of shock;and decreased to 3?10 -6 M PE after 4h of shock. The data suggested that contractile tension of VSM would decrease after shock and it maybe at least in part related to the decreased function of both calcium influx from ex cells and calcium release from sarcoplasmic reticulum of vascular smooth muscle cells.