To evaluate the forensic determination of post cerebral traumatic epilepsy. Methods In 21 patients, traumatic history and previous history were analysied combined with the demonstrations of electroencephalogram(EEG), X-ray, CT and MRI. Results Post-traumatic epilepsy,manily in late stage, usually occurred following serious cerebral trauma. The type of traumatic epilepsy was determined by the traumatic location and extent. Abnormal epileptic wave in scalp EEG and 24h dynamic EEG and medical image examinations were helpful for qualitative analysis. Conclusion The forensic determination of post traumatic epilepsy must be on the basis of traumatic and previous history combined with EEG, CT and MRI analysis.

The serous anti-corneum antibodies(ACAs)of 50 psoriatic patients and 32 normal con- trols were measured by Biotin Avidim Enzyme Linked Immunosorbent Assay (BA-ELISA).The results showed that ACAs of patients were significantly lower than that of the controls(P

Objective To investigate the effects of genistein on the epithelium-mesenchyme transition of alveolar type II cells; To discuss its mechanism of improving pulmonary fibrosis. Methods The activity of genistein with different concentrations and TGF-β1 intervention cells after 72 h were determined by CCK-8 method; epithelial and mesenchymal markers as well as the key regulatory factors in the transformation process were determined by RT-PCR; changes of p38 and JNK signaling pathways were determined by Western blot. Results The A549 cells were transformed into fibroblast phenotype stimulated by TGF-β1 for 72 h; mesenchymal markers increased and endothelial marker decreased. The results of RT-PCR indicated that genistein inhibited this phenomenon in a concentration-dependent manner. The results of Western blot showed that, genistein possibly inhibited the epithelial-mesenchymal transition through inhibiting the phosphorylation of p38 and JNK signaling pathways and its downstream transcription factors. Conclusion Genistein has anti-fibrosis effects through inhibiting A549 cells undergoing epithelial-mesenchymal transition.

Objective To find out the clinical characteristics related to neonate food allergy.Methods Ten subjects were diagnosed as neonate food allergy at the Third Hospital of Peking University from January 201 3 to January 201 5.The following clinical data:the gestational age,genter,age of onset,feeding,clinical manifestations,white blood cell count,eosinophilic granulocyte count,C -reactive protein(CRP),routine stool test and occult blood test,blood spe-cific IgE of cow′s milk and clinical outcomes were investigated.Results There were 4 term infants,and 6 preterm in-fants,including 2 very -preterm subjects.The gender ratio between male and female cases was 73.The age of onset from 1 day after birth to 43 days after birth,clinical investigation of neonate food allergy in 1 0 cases,and the average time was 20.3 d,the median age was 1 8.5 d,and the SD was 1 3.38 d.Their rectified gestational ages were from 30 to 37 weeks in 6 cases at onset.Four infants received breast feeding,3 infants received formula milk feeding,and the other 3 infants received mixed feeding.At onset,the symptoms were almost involved in the digestive system.The white blood cell counts of lab test were almost normal in 1 0 subjects.And eosinophilic granulocytes were elevated in 8 subjects.CRP elevated in 1 case,and those of the other 9 subjects were normal.The cow′s milk specific IgE antibody was elevated in 1 subject,and that of the other 9 subjects was normal.Two subjects were performed opened food challenge test and the finding were positive.Conclusions Both the term infants and the preterm infants can have food allergy,but the age of onset ranged widely.Breast feeding infants can also be attacked.The symptoms of the digestive tract were the main clinical manifestations of such neonates.The open -food challenge test is beneficial to diagnose but difficult to practice in the neonates.Dietetic treatment is the only method can therapy the disease.And mostly the clinical outcomes are desirable.

Objective: To investigate the clinical effect of mind-regulating acupuncture therapy on peripheral facial paralysis. Methods: Two hundred subjects were divided into observation group (treated by mind-regulating acupuncture therapy) and control group (treated by points of Yang-ming meridians) with one hundred in each group. The points of Yang-ming meridians were mainly selected for control group, observation group were treated by points with the function of regulating mind besides the points of control group, for two courses with 10 treatments in course. Results: The cure rate was 65.0% and total effective rate was 99.0% in observation group, while in control group, the cure rate was 55.0% and total effective rate was 91.0%, the curative effect in observation group was prior to control group (P＜0.05). Conclusion:The therapy of mind-regulating acupuncture gives a quicker response, a shorter course of treatment and a higher cure rate.

The factors influencing the integration of MOOC and information literacy education in academic libraries were divided into objective factor, subjective factor and social factor according to their connotation and significance. Certain measures for their integration were proposed, such as strengthening network construction, perfecting literacy education systems, and carrying out systemic training.Integration of MOOC and information literacy education in academic libraries can improve information service, promote wide application of MOOC and information literacy ed-ucation in academic libraries.

Objective To summarize the clinical data of patients with diabetic ketoacidosis (DKA), and improve the ability of diagnosis and treatment for patients with DKA. Methods The clinical data of 224 patients with DKA were retrospectively analyzed. Results After the positive diagnosis and comprehensive treatment including liquid-supplementing and small doses of insulin therapy, in 224 patients, 210 cases were cured, and the treatment success rate was 93.75%. Fourteen cases were dead, and the fatality rate was 6.25%. Conclusions Early diagnosis, liquid-supplementing and reasonable application of insulin therapy is the key to the success of DKA treatment.

Isocitrate dehydrogenases(IDHs)are considered as key enzymes in the tricarboxylic acid cycle. Recurrent mutations in the IDH1 and IDH2 genes are recently found in several human cancers. Those point mutations specifically affect IDH1 and IDH2 active site arginine residues and confer a neomorphic enzyme function of directly catalyzing α-ketoglutarate(α-KG)to R-2-hydroxyglutarate(R-2-HG). R-2-HG can com-petitively inhibits α-KG-dependent enzymes and may therefore contribute to the occurrence and development of tumor. In addition,Mutation status of IDH1 and IDH2 are closely relative to the progress and prognosis of cer-tain tumor. Thus IDH1 and IDH2 are considered to be promising biomarkers for early diagnosis and prognosis and targeted therapy.

Objective To investigate the effects of Genistein (GEN) acting on human lung adenocarcinoma A549 cells induced by LPS;To discuss its action of mechanism for improving injuries of lung. Methods The activity of cell treated with different concentrations of Genistein was detected by CCK-8 method and/or LPS intervention for 12 h. The expressions of inflammatory cytokines IL-1β, IL-6, and TNF-α induced by LPS were detected by RT-PCR. TUNEL was used to detect apoptosis, and Western blot was used to detect the changes of signal pathway. Results GEN (1, 5, 10μmol/L) alone had no effects on cell activity;LPS (1μg/mL) reduced cell viability significantly, while co-treated the A549 cells with GEN and LPS could reverse this condition in a concentration-dependent manner;RT-PCR results showed that LPS could significantly increase the level of gene expression of inflammatory factors, while GEN could inhibited this phenomenon in both concentration-and time-dependent manner;the results of TUNEL staining showed that GEN (10μmol/L) combined with LPS for 12 h could significantly decrease the apoptosis rate;the results of Western blot showed that GEN possibly inhibited the inflammation and apoptosis through inhibiting the phosphorylation of IκBα, p65 signaling pathways. Conclusion GEN has anti-inflammation and anti-apoptosis effects on A549 cells induced by LPS, so as to play a protective rate.