Child ; Child, Preschool ; China ; Drug Therapy ; standards ; Drug-Related Side Effects and Adverse Reactions ; Humans ; Infant ; Pediatrics ; Pharmacology, Clinical ; Pharmacovigilance ; Societies, Pharmaceutical

As one of the chronic diseases,asthma,plays a serious impact on human daily life.Asthma in children has showed an increasing trend in recent years,but the mechanisms of asthma are not yet clear.Studies have found that store-operated calcium entry(SOCE) plays an important role in the physiological activity of the body.The enhanced SOCE activity can promote cell growth,proliferation,and migration of a variety of cell types.SOCE important molecules STIM1 and ORAI1 may be involved in the asthmatic airway occurrence of hyperresponsiveness and airway remodeling,and closely to the asthmatic development.

Cytomegalovirus (CMV),a DNA herpusvirusesw,is the most common virus leading to intrauterine infetcion.It is transmitted from mother to fetus through placenta,and the neonates who suffering from congenital symptomatic infections may have sensorineural hearing loss,hepatosplenomegaly,microcephaly,chorioretinitis etc.Studies have suggested that the characters of infections on pregnant women,such as the time of infection,the serological results and virus load in amniotic fluid are associated with the outcome of offsprings.This paper is to review the risk of CMV infections on women at childbearing age,screening of CMV infections on pregnant women and the prenatal diagonosis of fetal CMV infection.

Objective To analyze the effect of Smad7 on the expression of TGF-?R,R Smads and STRAP in the process of malignant transformation of immortalized human bronchial epithelial cells BEP2D.Methods Cells were cultured and protein were extracted from BEP2D,BERP35T2,BS7 and RS7 cells,then Western blotting was performed to examine the expression level of TGF-?RⅠ,TGF-?RⅡ,Smad2/3,Smad4 and STRAP.(Results) Stable transfection of Smad7 gene led to decreased expression of TGF-?RⅡ and increased expression of Smad2 and STRAP in both BEP2D and BERP35T2 cells.There were no changes in the expression of Smad3 and Smad4.Conclusion Overexpression of Smad7 leads to decreased expression of TGF-?R Ⅱ and increased expression of STRAP in BEP2D and BERP35T2 cells,which could be one of the mechanisms of the malignant transformation of BEP2D cells.

[Objective] To observe the effect of electroacupuncture (EA) on learning and cerebral heme oxygenase (HO) activity in rats with vascular dementia (VD). [Methods] Sixty rats were randomly allocated to mimic group (Group A), EA group (Group B), nimotone group (Group C) and model group (Group D) . Rat models of VD were established by 4 - vessel occulusion (4 - VO) method. Ten days after modeling, Group B was trested with EA on Baihui (GV20) and Dazhui (GV14), one time per day; Group C with nimotone, 20ml/kg body weight, ig, qd. The treatment course lasted 15 days. And then the learning was estimated with Morris water maze and HO activity in cortex and hippocampus (HC) of rats was also measured. [Results] Mean escape latency within 6 days in EA group was shorter than that in model group ( P 0.05). In EA group, the swimming times in the original platform quadrant were more than that in the right, left or opposite quadrant and also more than that in the original platform quadrant in the model group (P

Objective:To discuss the different expression of AQP8 in cultured normal human trophocyte and JAR cell line,and the relationship between the changed expression of the channel protein and nosogenesis of chorionic carcinoma.Methods:The human cultured normal trophocyte was primarily cultured,purified and identified.JAR cell line was cultured.The expression of AQP8 was detected in cultured normal human trophocyte and JAR cell line by immunohistochemistry immunofluorescence confocal laser and RT-PCR.Results:(1)In the control group,the limitied expression of AQP8 was observed in cultured normal human trophocyte and JAR cell line,but in the exprimental group,the rate of positive cell of AQP8 in JAR cell line were 93.27%?14.45%,much higher than that in the control group.(2)By immunofluorescence staining confocal laser imaging,it was revealed that,in the same condition and excitation intensity,AQP8 in the JAR choriocarcinoma cell lines showed positive expression of the green,while in normal trophoblast cells,no green light,but when the excitation intensity was strengthened,it may still explore AQP8 in normal human villous trophoblast cell expression(.3)The expression of AQP8mRNA was detected in both cultured normal human trophocyte and JAR cell line.The OD value(0.98?0.35)of AQP8 in the experimental group was significantly higher than that(0.32?0.04)in the control group.Conclusion:Abnormal overexpression of AQP8 will destroy the external water and electrolyte balance of trophoblast cells,which might be one of the reasons for the occurrence of choriocarcinoma.

Minimal residual disease(MRD)iS the origin of relapse in leukemia.So it's significant to quantitate MRD in clinical.At present, there are three MRD quantitative assays, such as flow cytometric detection of abnormal immuno-phenotype,polymerase chain reaction(PCR)amplification of antigenreceptor genes and fusion transcripts.Because every measure has its own principle and feature.the clinical applications are different.Selecting suitable assays based on the characteristic of patients can help to quantitate MRD exactly.

Child ; Environmental Pollution ; Feeding Behavior ; Humans ; Lead ; pharmacokinetics ; Lead Poisoning ; diagnosis ; etiology ; Sanitation

The research on the clinical characteristic and epidemiology in Henoch-Schonlein purpara of children indicates that genetic factors are closely connected with the disease and pathological process.In recent years,molecular biology studies show that C1 GALT1 gene,IL gene,vasomotor and endothelial function regulation genes,angiotensin-converting enzyme gene,angiotensinogen gene,MEFV gene and so on,which have aberrant IgAl giycosylation,are closely related with pathogenesis of Henoch-Schonlein purpura in children.The paper reviews the progress of genetic mechanism associated with Henoch-Schonlein purpura in recent years.

In recent studies,it has been demonstrated that the clinical efficacy of surfactant is poor for late protein infants.Besides,this kind of infants have not infection or other risk factors that may lead to respiratory distress syndrome(RDS).Thus,it is speculated that the pathogenesis of RDS of late preterm infants is different from preterm infants.New researches show that,as the key point of Lung fluid transport via Epithelium,α-ENaC plays an important role in the pathogenesis of RDS of late preterm infants through the inhibition of lung fluid clearance and activity of surfactant.This review highlights the most important mechanisms of Lung fluid absorption barrier in RDS of late preterm infants.