Objective:To evaluate the effects of smokeless tobacco extract(ST) on number,morphology,ultrastructure and proliferation of human gingival fibroblasts(HGFs) on titanium in vitro.Methods:HGFs on titanium were cultured in the presence of ST at various concentrations.The cell changes in the morphology and ultrastructure were examined by scanning electrical microscope(SEM).The growth and attachment of the cells were measured by MTT method.Results:The size of the cells became smaller gradually and their shapes changed from spindle type to oval or round when the concentrations of ST increased.Ultrastructure showed that pseudopod decreased in number,the growth and attachment were dose-dependently inhibited.Conclusion:ST can change number,morphology and ultrastructure of HGFs on titanium,they can inhibit the cells growth,suggesting ST may play a pathological role on implant-gingiva interface.

The purpose of applying antibiotics prophylaxis in perioperative orthopedic surgery is to prevent or decrease the risk of infection.The opportune administration is the key point for prevention of infection.The application principle,classification and current situation are reviewed in this paper,its perspective and prospects are also investigated.

Objective To investigate the long-term outcome of intra-arterial thrombolysis in patients with acute ischemic stroke and its influencing factors.Methods Patients with acute ischemic stroke received intra-arterial thrombolysis were included in the study.The neurological outcome at day 90 was assessed using the modified Rankin Scale (mRS).They were divided into the good outcome group (mRS scores,0 to 2) and the poor outcome group (mRS scores,3 to 6)according to the evaluation results; the degree of recanalization after thrombolysis was assessed by the grading criteria of the Thrombolysis in Myocardial Infarction (TIMI) trial; the incidence of intracerebral hemorrhage within 7 days after thrombolytic therapy and the mortality at 3months were recorded.Univariate analysis and multivariate logistic regression analysis were used to screen the influencing factors of long-term outcome of arterial thrombolysis.Results A total of 42 patients were included,of them,19 (45.2％) with good outcome and 23 (54.8％) with poor outcome after intra-arterial thrombolysis; 27 patients (64.5％ ) with good recanalization (TIMI grade,2 to 3); 13 patients (31.0％) occurred intracranial hemorrhage within 7 days,and 8 of them (19.0％ ) had symptomatic intracranial hemorrhage; 11 (26.2％ ) died within 90 days.Univariate analysis showed that the baseline blood glucose levels (P=0.019),the baseline National Institutes of Health Stroke Scale (NIHSS) scores (P =0.014),symptomatic intracranial hemorrhage (P =0.005),and the degree of recanalization (P =0.002) could influence the longterm outcome of patients with intra-arterial thrombolysis.Multivariate logistic regression analysis indicated that the lower level of basdine glucose and good recanalization were the independent predictive factors of the good long-term outcome after intra-arterial thrombolysis in patients with acute ischemic stroke.Conclusion After the exclusion of contraindications,the intra-arterial thrombolysis was safe and effective for patients with acute ischemic stroke.The lower blood glucose levels on admission and the good recanalization after thrombolysis were associated with the good long-term outcome of intm-arterial thrombolysis.

Objective:To investigate the effects of sodium phenylbutyrate(NaPB) on the matrix metalloproteinase-9(MMP-9) and tissue inhibitor of metalloproteinase-1(TIMP-1) expression and invasive ability of human thyroid follicular carcinoma cell line CGTHW-1.Methods: CGTHW-1 cells were treated with different concentrations of NaPB,then the invasive ability of CGTHW-1 cells was assessed using Transwell assay.The expression of MMP-9 and TIMP-1 was examined by immunocytochemistry staining and RT-PCR in CGTHW-1 cells.Results: After treatment with NaPB(4 mmol/L) for 72 h,CGTHW-1 cells passing the Transwell were significantly reduced[(29.8?1.77) vs(11.00?2.59),P

In the past, panretinal photocoagulation (PRP) and vitrectomy (PPV) were the main treatments for proliferative diabetic retinopathy (PDR). In recent years, anti-vascular endothelial growth factor (VEGF) drugs have been used more and more widely in PDR due to their advantages in rapidly subtracting new blood vessels, reducing leakage, and promoting the absorption of blood. The combination of anti-VEGF drugs and PRP in the treatment of PDR, especially high-risk PDR, can increase the rate of neovascularization and prevent some patients with mild to moderate vitreous hemorrhage from PPV. The application of anti-VEGF drugs during the perioperative period of PPV can also reduce bleeding during the operation, shorten the operation time, and reduce surgical complications. Although clinical studies have confirmed that anti-VEGF drugs can be used as an alternative treatment for PRP, most patients require multiple and long-term treatments, which increase the psychological and economic burden of patients. It is expected that the cost of anti-VEGF drugs and the development of long-acting dosage forms can be reduced and bring better efficacy and benefits to PDR patients in the future.

Carcinosarcoma ; Female ; Humans ; Maxillary Sinus Neoplasms ; Middle Aged

Objective To investigate the value of digital image analysis (DIA) of endoscopic ultrasonography (EUS) in the differential diagnosis of benign and malignant pancreatic neuroendocrine neoplasm (PNEN).Methods The relation between various parameters of EUS-imaging and benign and malignant lesions in 47 patients clinically diagnosed PNEN were retrospectively analyzed.Photoshop CS5 software was performed for digital image processing,and lesions related parameters were collected,including area,perimeter,length,circularity,gray,gray ratio,and gray standard deviation.The statistical method of t test was performed for comparison between two groups.Receiver operating characteristic (ROC) curve was analyzed in length,circularity,average gray scale ratio and gray standard deviation.Results Among the 47 patients,35 cases and 12 cases were in benign group and malignant group,respectively.The mean gray scale ratio and the circularity of benign group were significantly higher than those of malignant group (0.80±0,05 vs 0.74±0.07,0.63±0.17 vs 0.40±0.09),and the differences were statistically significant (t=2.659 and 5.787,both P＜0.05).The gray standard deviation of benign group were lower than that of malignant group (9.90 ± 1.24 vs 12.55± 3.27),and the difference was statistically significant (t=-2.733,P=0.018).The area under the curve (AUC) of circularity was 0.724 (95％ confidence interval(CI):0.546 to 0.901),the cut-off value was 0.767,the sensitivity and specificity were 71.43 ％ and 66.67％,respectively.The AUC of average gray ratio was 0.888 (95％CI:0.785 to 0.991),the cut-off value was 0.412,the sensitivity and specificity were 94.29％ and 75.00％,respectively.The AUC of gray standard deviation was 0.811 (95％CI:0.647 to 0.974),the cut-off value was 11.02,the sensitivity and specificity were 66.67％ and 85.71％,respectively.When combined with the three parameters of circularity,average gray scale ratio and gray standard deviation,the sensitivity and specificity were 97.14％ and 83.33％,and the accuracy was 93.61％.Conclusions EUS with DIA technology can improve the detection of EUS images to PNEN,and which may be complementary to EUS guided fine needle aspiration.It also privided a noninvasive,objective,convenient,and effective option for the differential diagnosis of benign and malignant PNEN.

[Objective]To discuss the clinical results and treatments of the Monteggia's fracture in children.[Method]Total of 66 patients including 61 fresh and 5 old fractures were reviewed.There were 47 males and 19 females.According to the Bado classification system: 46 of type Ⅰ,4 of type Ⅱ,15 of type Ⅲ,1 of type Ⅳ,all patients received conservative or operatire treatment.The conservatively treated patients received manipulation and plaster immobilization.In the operative group,the patients were fixed with single or/and double Kirschner's wires after reduction of the radiohumeral joints or/and fractured ulna.Neither reparation nor reconstruction was needed.[Result]All patients were available at the final follow up with 0.5～5 years.According to the Li Hanmin's scoring system for the motion function in children: excellent and good rate was 95.7% in conservative group and 84.2% in operative group.There was no complication such as myositis ossificans,synostosis of radius and ulna,ulna nonunion,radial head dislocation and profundus radial nerve injury.The cases of nerve injury were complete recovered after operation two or four months later.[Conclusion]The clinical results of the Monteggia's fracture in children was very good with immediate reduction and internal fixation in proper cases,a good overall therapeutic outcome can be expected in children with Monteggia's fracture.It is very important to prevent missed diagnosis.

[Objective]To explore the clinical effect of the new stem-less total hip arthroplasty.[Method]Exposed the hip joint through posterior-exterior incision,dislocate the femoral head,clear away sick tissues inside joint,throw into the centric pin by guide of the centric localizer,repaired bone by machine of modeling bone,set the cup and fixed by centric screw and the screw of substantia spongiosa bone on trochanter major and trochanter minor of the femoral.The acetabulum prosthesis according to the traditional total hip arthroplasty were fixed.[Result]Nine cases were followed up for average 20 months,evaluated by Harris Score standard,excellent 5 cases,good 2 cases,fair 1 cases,poor 0 case,the rates of excellent and good is 88.89%.[Conclusion]Because of keeping entire neck and part head of the femur,biomechanics conduction and distribute of the stem-less hip of prosthesis achieves natural biomechanics state of the human body,the treatment effect of short-term is satisfying,the treatment effect of long-term will go on get observation in future.

Objective To evaluate influencing factors of the long-term maintenance therapy in patients with gastroesophageal reflux disease (GERD).Methods 156 Patients with GERD were divided into on-demand therapy group and continuous therapy group by their treatment,according to symptom relief and improvement of quality of life in the course of therapy.The relationship between treatment and course,symptoms, esophageal motility,24-h pH monitoring and endoscopic findings were analysed.Results (1)5 Patients showed no response to acid suppression therapy and were excluded.For the 151 patients,92 were in on-demand therapy group,and 59 were in continuous therapy group.(2)The disease course and symptoms score in on-demand therapy group were significantly longer and higher than those of continuous therapy group (P