Objective To explore the role of leptin,COX-2 and p27 in bile-induced gastric mucosal injury in rats.Methods SD rats were divided into 4 groups: DGR(duodenogastric reflux) group,DGR+BDL(ligation of bile duct) group,BDL group and control group.The pathological changes of gastric mucosa and tight junction were observed,DIXON scores were warked out after 3 months.Immunohistochemistry was used to detect the expression of leptin,COX-2 and p27 in gastric mucosa with different lesions. Results The DIXON scores in DGR and DGR+BDL group were higher than those in BDL group and control group(P

Cotinine is the best biomarker for the assessment of the exposure to environmental tobacco smoke(ETS).With the"Framework Convention on Tobacco Control"implemented in China,the hazards from ETS are increasingly concerned,the determination of cotinine in human body which reflects the internal exposure to ETS received universal recognition day by day.It is imperative to create a standardized,simple and fast method to detect cotinine in human body.The recent determination techniques of cotinine,the application in different human samples and the direction of development were introduced in the present paper.

AIM:To study the sedation, diuresis and anticoagulation effects of Ji-Ming-San (JMS) in animal models for exploring the circadian variations of the dosing action and verifying the rationality of administering JMS on special time. METHODS: The sedation effect and circadian difference were determined by observing the time session of ambulation, raising double-forefoot test and autonomic activity of model mice. The diuretic effect and the circadian variation in rats were examined by metabolic cage test while the anticoagulation and the circadian change were observed by glass test. RESULTS: JMS produced significant sedation effect by reducing time session of ambulation, raising upper limbs frequencies/time and autonomic activity and the built-in rhythm with autonomic activity disappeared after JMS administration. JMS had satisfactory diuretic effect and total urinary output in water-loaded rats was increased after dosing. The diuretic effect of dosing showed a circadian rhythm with more significant output during the night than that during the daytime. Also, JMS prolonged the clotting time significantly and the action exhibited circadian difference. As compared with administration at the night, the clotting time was more prolonged at the daytime. CONCLUSION: JMS can produce obvious sedation, diuresis and anticoagulation effects with varied circadian rhythm. The findings suggest that the effect of administering JMS is better at the end of rest phase than other time session.

Keratocytes situated between the collagen lamellae in the corneal stroma,are mitotically quiescent cells.The cells can secrete collagens and keratan sulfate proteoglycans which contribute to the transparency of the cornea.Upon injury,keratocytes are stimulated to corrent apoptosis and transite into repair phenotypes,fibroblast and myofibroblast.These repair phenotypes can either promote regeneration or induce fibrotic scar,and the latter is detrimental to the otherwise transparent cornea.Moreover,a population of keratocytes can be induced to appear the stem cell-like characteristics.Therefore,keratocytes are no longer bystanders,but active participants in numerous functions.

Objective To evaluate clinical results of endoscopic vein harvesting(EVH) in patients who underwent coronary artery bypass grafting(CABG).Methods From July 2013 to October 2014,500 patients who underwent isolate CABG in our institution were divided into two groups:EVH group (n =250) and open vein harvesting(OVH) group(n =250).The surgical result and perioperative complications were compared between the two groups.Results The procedures were completed successfully in all the patients in both groups.There was no significant difference existed in the length of the harvested vein and vein injury between the two groups(P ＞ 0.05).Similar results were obtained in the in-hospital mortality.Compared to group OVH,group EVH showed significantly shorter harvesting time,smaller incision,and lower rates of perioperative leg complications (P ＜ 0.01).Group EVH had a less bed staying time after operation and a shorter post-operation hospital stay (P ＜ 0.05).EVH group achieved an improved patient satisfaction and postoperative pain when compared with OVH in CABG(P ＜0.05).Conclusion EVH can provide adequate vein graft for CABG,and also significantly reduce wound related complications when compared with OVH.EVH is recommended for vein harvesting to improve patient satisfaction.

OBJECTIVE:To observe the efficacy and safety of vitamin A acid combined with vitamin A,vitamin E,celecoxib in the treatment of oral leukoplakia. METHODS:104 patients with oral leukoplakia were randomly divided into control group(52 cases) and observation group (52 cases). Control group received 1 Vitamin A soft capsule,3 times a day+1 Vitamin E soft cap-sule,3 times a day+1 Celecoxib capsule,twice a day. Observation group additionally received 1 Vitamin A acid tablet,twice a day. 4-week was regarded as 1 treatment course,it lasted for 3 courses. Clinical efficacy,and leukoplakia number,maximum diam-eter of leukoplakia,immunoglobulin A(IgA),IgM,IgG before and after treatment,and the incidence of adverse reactions in 2 groups were observed. RESULTS:The total effective rate in observation group was significantly higher than control group,the dif-ference was statistically significant(P<0.05). Before treatment,there were no significant differences in leukoplakia number,maxi-mum diameter of leukoplakia,immunoglobulin levels in 2 groups(P>0.05). After treatment,leukoplakia number,maximum diam-eter of leukoplakia,IgA and IgM in 2 groups were significantly lower than before,and observation group was lower than control group,IgG was significantly higher than before,and observation group was higher than control group,the differences were statisti-cally significant (P<0.05). And there was no significant difference in the incidence of adverse reactions in 2 groups (P>0.05). CONCLUSIONS:Vitamin A acid combined with vitamin A,vitamin E,celecoxib shows obvious efficacy in the treatment of oral leukoplakia,it can effectively reduce clinical symptoms,improve immune function,with good safety.

AIM:To observe effects of Jiming Powder(Semen arecae,Pericarpium citri reticulatae,Fructus Chaenomelis,Fructus Evodiae,Folium Perillae,Radix Platycodonis,and Rhizoma Zingiberis recens) on anticoagulation and hemorheology and on the circadian variations in order to verify the rational practice of timing administering the drug by our ancestors. METHODS:The effect of Jiming Powder on clotting time(CT) was observed by using glass-tube method in mice. Quick test was used to determine the prothrombin time(PT) and circadian variations in rats. The experimental animal models of blood stasis syndrome were prepared by subcutaneous injection of adrenaline in combination with ice water bath for observing the effect of Jiming Powder on hemorheology in such types of animals,and the maximum rate of platelet aggregation was determined by method of Born turbidimetry. RESULTS:Jiming Powder could significantly delay CT and PT characteristic of circadian rhythm. As compared with the administration at daytime,CT and PT were greatly prolonged during the night. Besides,the hemorheological parameters were improved markedly in blood,plasma and fibrinogen viscosity,which suggested that the drug administration was more effective during the night than daytime. Also,the findings showed that Jiming Powder could prevent platelet aggregation induced by adenosine diphosphate. CONCLUSION:Jiming Powder plays a role in anticoagulation and improves the indication of hemorhelogy in the blood-stasis rats model,showing dosing time-dependent.

Objective Toinvestigatetheeffectoffolicacidonplasmahomocysteine(Hcy)levels and vascular endothelial function in elderly cerebral infarction patients with hyperhomocysteinemia. Methods Thisstudywasaprospectivecontrolledtrial.Atotalof112elderlylacunarinfarction patients with high hyperhomocysteinemia admitted to the Department of Neurology,Nanjing Municipal Government Hospital from November 2012 to October 2013 were enrolled. The patients were divided into two groups according to the method of random number table. The patients of both groups received the conventional drug treatment of ischemic stroke at the same time. Fifty-six patients were in group A,and they were treated with folic acid 20 mg,once a day;56 patients were in group B,and they did not treat with folic acid. The Hcy levels on admission and after 24 weeks treatment were measured. Brachial artery flow-mediated endothelium-dependent dilation (FMD)was measured by high resolution ultrasound,and nitric oxide (NO), nitricoxidesynthase(NOS),andendothelin(ET)weredetectedatthesametime.Results Twenty-four weeks after treatment,the plasma Hcy level in group A was significantly decreased as compared with prior treatment (12. 2 ± 4. 2 vs. 22. 6 ± 3. 7μmol/L;P<0. 05),moreover,it was lower than the level of group B at the same period (12. 2 ± 4. 2 vs. 21. 8 ± 4. 0μmol/L;P <0. 05 ). The FMD level of group A was significantly higher than that of prior treatment and group B (11. 2 ± 3. 6 vs. 9. 2 ± 3. 3,9. 3 ± 3. 3;P<0. 05). The NO and NOS levels of group A at 24 weeks after treatment were significantly higher than those of prior treatment and group B at the same period (76. 2 ± 9. 4 vs. 49. 2 ± 9. 0,50. 0 ± 9. 1μmol/L,47. 6 ± 9. 5 vs.38. 4 ± 7. 5,37. 8 ± 8. 8 kU/L;P<0.05). The ET level of group A was decreased as compared with that of prior treatment and group B at the same period (81. 3 ± 20. 5 vs. 105. 6 ± 25. 2,105. 3 ± 21. 8 ng/L;P<0.05).NoadversereactionwasfoundduringthetreatmentingroupA.Conclusion Alargerdoseof folic acid can significantly reduce the plasma Hcy level,decrease the FMD level,and ameliorate vascular endothelial function in elderly cerebral infarction patients with hyperhomocysteinemia.

Two-way choice is the main way for the seven-year-program clinical medical students and postgraduate supervisors.This article analyzes the influence factors and shortages of two-way choice for postgraduate supervisors and seven-year-program clinical medical students.This investigation discovers that the supervisor's abilities of research,teaching and clinical and their personal magnetism are important and key factors.The students' gender,character,hobbies and interests and the familiarity to profession are the secondary factors.The other factors that include employment status,the level of income and the advice of parents and friends also have influence on two-way choice for postgraduate supervisors and seven-year-program clinical medical students.In order to further improve the tow-way choice,the author gives some suggestions,including introducing profession and the scientific researches of supervisors in advance,encouraging the face-to-face communication between students and supervisors,promoting multiple profession choices and giving some aids to the students for choosing the supervisors.

Objective To learn the change of serum thymidine Kinase 1(TK1) level in the treatment process of the early cure nasopharyngeal carcinoma patients and its relationship to pathological features .Methods From January 2010 to December 2013 ,35 cases of nasopharyngeal carcinoma patients were collected into our study ,20 cases of nasopharyngeal inflammation patients were chosen as the control .Serum TK1 level in the 2 groups was compared before and after treatment .Results TK1 in control group had no significant differences before and after treatment (P>0 .05) .Compared with the control group ,serum TK1 in nasopharyngeal carcinoma group were higher before and after radiotherapy (P<0 .05);after radiotherapy ,serums TK1 in nasopharyngeal carcinoma group were lower(P<0 .05) .Before and after radiotherapy ,serum TK1 Ⅰ and Ⅱ stage were lower than in Ⅲ and Ⅳ stager(P<0 .05) ,lymph node metastasis group were higher than no lymph node metastasisr (P<0 .05) ,TK1 had higher expression in TNMⅣ than TNM Ⅱ(P<0 .05) ,compared with lymph node metastasis and cervical nerve violated groups ,TK1 expression was higher in none lymph node metastasis and none cervical nerve violated groups (P<0 .05) .Conclusion TK1 can reflect the tumor load and proliferative activity of nasopharyngeal carcinoma ,which could be as a factor in nasopharyngeal carcinoma diagnosis ,monitoring and prognosis .