Objective Serum pharmacological assessment:the effect of Rubia yunnanensis extract on the immune function stimulated by superantigens of streptococcus. Methods Using cell culture technique, mouse spleen lymphocytes and peritoneal macrophages were culture with superantigens of streptococcus and serum of Rubia yunnanensis. Lymphocytes proliferation stimulated by superantigens of streptococcus measured by MTT assay. NO level in mouse peritoneal macrophages affected by Rubia yunnanensis at different concentrations were determined and compared. Results Serum of Rubia yunnanensis significantly inhibit the proliferation of lymphocytes stimulated by superantigens of streptococcus, compared with normal control. NO content of macrophages culture in Rubia yunnanensis extract group were lower than those of control. In these tests, the effect of the extract by alcohol is more obvious than that of extract by water. Conclusions Rubia yunnanensis extract has effect in inhibiting immune function and anti-infect activity, it could be applied in clinical practice for immunoregulation and treatment of psoriasis.

Individual dose monitoring and registry for radiation workers plays a significant role in ionizing radiation protection and occupational health management. Since the nationwide implementation of monitoring from the 1980s, individual dose monitoring for radiation workers in China has been going on nearly 30 years, which is of significance in radiation protection optimization, and occupational health and protection for radiation workers. In order to improve individual dose monitoring and registry, to prevent and control occupational radiation diseases, and to help build "Healthy China" program, this paper presents the evolution of individual dose monitoring and registry in China, discusses the key points and difficulties faced, and proposes suggestions.

Objective　To summarize the phenotypic and genotypic characteristics of mitochondrial combined oxidative phosphorylation deficiency type 1 (COXPD1), and to improve the clinicians' awareness of this mitochondrial encephalomyopathy. Methods　The clinical characteristics, physical examination, laboratory examination and other data of a child with COXPD1 were analyzed retrospectively. The diagnosis was confirmed by clinical whole exon sequencing and high-precision mitochondrial genome full-length PLUS gene detection, and the phenotype and genotype were analyzed by reviewing relevant literature. Results　A one-year and five-month-old boy mainly presented with hyperlactacidemia and abnormal liver function. Clinical whole exon sequencing showed that the child had homozygous variation of c. 688G>A(p.G230S) in the GFM1 gene. Sanger sequencing verified that the variation was respectively inherited from the parents of the child (both were heterozygous) with the autosomal recessive inheritance pattern. The high-precision mitochondrial genome full-length PLUS detection also did not find pathogenic mutations related to clinical phenotypes. The child was diagnosed with COXPD1. After "cocktail" therapy and liver protection therapy, the patient's condition improved. Conclusions　The phenotype of COXPD1 is complicated and variable, mainly liver type and brain type. The mutation of GFM1 gene affects mitochondrial translation system function, and early gene detection is helpful for definite diagnosis.

Lysimachiae Herba and Desmodii Styracifolii Herba are common traditional Chinese medicines for treating lithiasis. Both of them have efficacies of clearing heat, diuresis and eliminating calculi. However, there are some differences in their clinic applications. The former is mainly used to treat hepatolithiasis, gallstones, jaundice, stranguria and gout; Whereas the latter is mainly used to treat urinary calculus. In this paper, the pharmacological effects of Lysimachiae Herba and Desmodii Styracifolii Herba on removing calculus, choleresis, anti-inflammation and oxidation resistance were compared and analyzed based on document retrieval. In conclusion, both of them show the preventive and therapeutic effects on kidney stones and gallstones. Particularly, Desmodii Styracifolii Herba has a better effect in treating the kidney stones, while Lysimachiae Herba has a better effect in treating cholesterol gallstones.
Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Fabaceae ; Gallstones ; drug therapy ; Humans ; Kidney Calculi ; drug therapy ; Medicine, Chinese Traditional ; Phytotherapy ; Primulaceae

New military transformation leads to the transformation of the mechanism of military medical service,and stimulates the development of reserve hospitals.Based on its unique function of from basic medicine to clinical medicine,from innovation to practice,from research to application,translational medicine combines the concept of base on market and face battlefield,it has satisfied the need of an all-round development.Technical innovation is the core of translational medicine,it is also the basic way to meet the growing demand of medical support in war time.Thus,to strengthen the construction of the technical innovative system of translational medicine not only provides a new guidance to the development of reserve hospitals,but also offers an important guarantee to the realization of effective hospital mobilization and medical relief effort.

Objective:To observe the effects of a preparation of chinese traditional medicine Zengshengping (ZSP) in the treatment of oral lichen planus (OLP).Methods:200 patients with OLP were randomly divided into 2 groups with 100 in each.The patients in one group were treated with ZSP and those in another with levomisolum.The patients were followed up for two years.Results:The total effective rate in ZSP or levomisolum treated group were 90% and 62% respectively (P<0.025).Conclusion:ZSP is more effective in the treatment of OLP than levomisolum.

Objective To investigate the effect of IGF-1 on the TGF-β3 induced chondrogenesis of MSCs encapsulated in alginate beads and its application in cartilage tissue engineering.Methods MSC chondrogenesis in alginate beads was induced by TGF-β3 and/or IGF-1.Collagen type Ⅱ,aggrecan and Sox-9 expression was evaluated by immunostaining,RT-PCR and Western blot,respectively.Scanning electron microscope and laser confocal microscope were used to observe the differentiated chondrocytes when cultured on the chitosan-based scaffold.Results TGF-β3 with IGF-1 induced MSCs in alginate beads to express the higher level of collagen type Ⅱ,aggrecan and Sox-9 than any other growth factor alone (P＜0.05).The correlation coefficient between Sox9 and collagen type Ⅱ or aggrecan was 0.95 and 0.91,respectively.The chitosan-based scaffold supported the cell's adhesion,migration and proliferation.Conlusion IGF-1 enhances the TGF-β3-induced MSC chondrogenesis via upregulating Sox9 expression.The chitosan-based scaffold is biocompatible with the differentiated chondrocytes.

Objective To investigate the clinical effects of surgery treatment of the Tibia plateau fracture.Methods Thirty-two cases diagnosed Tibial plateau fracture were performed internal fixation and bone grafting surgery treatment.Results According to Rasmussen Evaluation,there were the gifted 22 cases(68.8%)excellent,6 cases(18.8%)good,2 cases(6.3%)ordinary,2 cases(6.3%)poor.The good rate Was 87.6%.Conclusions Surgery is effective to trot Tibial plateau fracture.A stable internal fixation,as far as possible,reduces the blood supply to fracture block interference and protects joint subsidiary structure to correct joint function rehabilitation treatment.

Objective To make an inquiry into how to reconstruct a lifelike ear, and which is one of the most thorny problem in plastic surgery. Methods The operation was divided into two stages: the first stage was that an expander was put into a patient's opisthotic subscutaneous region and lobe of the ear was transposed or laid aside; the second stage was that the expander was taken off by a 2.5～3.0 ㎝ incision at the top or bottom of the expanded flap. The reniform incision except the preauriclar pedical part which was 0.5～0.6 ㎝ away from the expander edge was slit until the deep fascia and dissected forword at the same layer in order to form a "bag-shape" flap. Then, an auricle model was put and fixed and aspirating tube was inserted in the "bag". The back of the reconstructive ear was repaired with the full thickness graft. Results It made operating procedure simple, negative pressure plasty looked good, the blood circuit of flap was increased and occurrence rate of complication reduced. The operation had been performed on 18 patients with a satisfactory results. Conclusion The success rate of the procedure is higher and the shape of the reconstructed ear is more lifelike than that of the traditional one.

Osteoarthritis (OA) is a kind of chronic and frequently-occurring disease in the elderly.It is also called degenerative disease.Pain,stiffness,swelling,deformity and dysfunction of joints are the main clinical manifestations,which seriously affect the quality of life.Kaschin-Beck Disease (KBD) is a kind of endemic diseases;its main feature is cartilage degeneration and necrosis.Although KBD becomes a typical OA in late stage,it is different from OA.Osteoprotegerin (OPG) and insulin-like growth factor (IGF)-1 participate in the bone growth,repair,and alteration.In this paper,we summarized clinical manifestations and pathological changes of OA,and reviewed the current situation and development of OPG and IGF-1 in OA bone remodeling.