1.Serum Immunopharmacological Assessment of Effects of Rubia Yunnanensis on Murine Immune Cell Stimulated with Streptococcal Antigens
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(02):-
Objective Serum pharmacological assessment:the effect of Rubia yunnanensis extract on the immune function stimulated by superantigens of streptococcus. Methods Using cell culture technique, mouse spleen lymphocytes and peritoneal macrophages were culture with superantigens of streptococcus and serum of Rubia yunnanensis. Lymphocytes proliferation stimulated by superantigens of streptococcus measured by MTT assay. NO level in mouse peritoneal macrophages affected by Rubia yunnanensis at different concentrations were determined and compared. Results Serum of Rubia yunnanensis significantly inhibit the proliferation of lymphocytes stimulated by superantigens of streptococcus, compared with normal control. NO content of macrophages culture in Rubia yunnanensis extract group were lower than those of control. In these tests, the effect of the extract by alcohol is more obvious than that of extract by water. Conclusions Rubia yunnanensis extract has effect in inhibiting immune function and anti-infect activity, it could be applied in clinical practice for immunoregulation and treatment of psoriasis.
2.Mitochondrial combined oxidative phosphorylation deficiency type 1 in childrena case report and literature review
China Tropical Medicine 2023;23(2):200-
Objective To summarize the phenotypic and genotypic characteristics of mitochondrial combined oxidative phosphorylation deficiency type 1 (COXPD1), and to improve the clinicians' awareness of this mitochondrial encephalomyopathy. Methods The clinical characteristics, physical examination, laboratory examination and other data of a child with COXPD1 were analyzed retrospectively. The diagnosis was confirmed by clinical whole exon sequencing and high-precision mitochondrial genome full-length PLUS gene detection, and the phenotype and genotype were analyzed by reviewing relevant literature. Results A one-year and five-month-old boy mainly presented with hyperlactacidemia and abnormal liver function. Clinical whole exon sequencing showed that the child had homozygous variation of c. 688G>A(p.G230S) in the GFM1 gene. Sanger sequencing verified that the variation was respectively inherited from the parents of the child (both were heterozygous) with the autosomal recessive inheritance pattern. The high-precision mitochondrial genome full-length PLUS detection also did not find pathogenic mutations related to clinical phenotypes. The child was diagnosed with COXPD1. After "cocktail" therapy and liver protection therapy, the patient's condition improved. Conclusions The phenotype of COXPD1 is complicated and variable, mainly liver type and brain type. The mutation of GFM1 gene affects mitochondrial translation system function, and early gene detection is helpful for definite diagnosis.
3.Individual dose monitoring and registry for radiation workers in China
Chinese Journal of Radiological Medicine and Protection 2021;41(2):81-84
Individual dose monitoring and registry for radiation workers plays a significant role in ionizing radiation protection and occupational health management. Since the nationwide implementation of monitoring from the 1980s, individual dose monitoring for radiation workers in China has been going on nearly 30 years, which is of significance in radiation protection optimization, and occupational health and protection for radiation workers. In order to improve individual dose monitoring and registry, to prevent and control occupational radiation diseases, and to help build "Healthy China" program, this paper presents the evolution of individual dose monitoring and registry in China, discusses the key points and difficulties faced, and proposes suggestions.
4.Comparison between Lysimachiae Herba and Desmodii Styracifolii Herba in pharmacological activities.
Ying XIONG ; Jun-wen WANG ; Jun DENG
China Journal of Chinese Materia Medica 2015;40(11):2106-2111
Lysimachiae Herba and Desmodii Styracifolii Herba are common traditional Chinese medicines for treating lithiasis. Both of them have efficacies of clearing heat, diuresis and eliminating calculi. However, there are some differences in their clinic applications. The former is mainly used to treat hepatolithiasis, gallstones, jaundice, stranguria and gout; Whereas the latter is mainly used to treat urinary calculus. In this paper, the pharmacological effects of Lysimachiae Herba and Desmodii Styracifolii Herba on removing calculus, choleresis, anti-inflammation and oxidation resistance were compared and analyzed based on document retrieval. In conclusion, both of them show the preventive and therapeutic effects on kidney stones and gallstones. Particularly, Desmodii Styracifolii Herba has a better effect in treating the kidney stones, while Lysimachiae Herba has a better effect in treating cholesterol gallstones.
Drugs, Chinese Herbal
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pharmacology
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therapeutic use
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Fabaceae
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Gallstones
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drug therapy
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Humans
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Kidney Calculi
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drug therapy
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Medicine, Chinese Traditional
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Phytotherapy
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Primulaceae
5.A clinical observation of the effect of Zengshengping therapy for oral lichen planus
Jun CAO ; Rujun ZHOU ; Haiping DENG
Journal of Practical Stomatology 2001;17(1):50-51
Objective:To observe the effects of a preparation of chinese traditional medicine Zengshengping (ZSP) in the treatment of oral lichen planus (OLP).Methods:200 patients with OLP were randomly divided into 2 groups with 100 in each.The patients in one group were treated with ZSP and those in another with levomisolum.The patients were followed up for two years.Results:The total effective rate in ZSP or levomisolum treated group were 90% and 62% respectively (P<0.025).Conclusion:ZSP is more effective in the treatment of OLP than levomisolum.
6.Surgical treatment of Tibia plateau fracture:a clinical analysis of 32 cases
International Journal of Surgery 2008;35(7):465-467
Objective To investigate the clinical effects of surgery treatment of the Tibia plateau fracture.Methods Thirty-two cases diagnosed Tibial plateau fracture were performed internal fixation and bone grafting surgery treatment.Results According to Rasmussen Evaluation,there were the gifted 22 cases(68.8%)excellent,6 cases(18.8%)good,2 cases(6.3%)ordinary,2 cases(6.3%)poor.The good rate Was 87.6%.Conclusions Surgery is effective to trot Tibial plateau fracture.A stable internal fixation,as far as possible,reduces the blood supply to fracture block interference and protects joint subsidiary structure to correct joint function rehabilitation treatment.
7.Dynamic changes of the expression of HIF-1,VEGF and miRNA-210 in the kidney after unilateral ureteral occlusion
Jun DENG ; Renrui KUANG ; Suping CUI
Chongqing Medicine 2015;(30):4196-4198
Objective To observe the expression of inducible factor 1(HIF‐1) ,vascular endothelial growth factor(VEGF) and miRNA‐210 and explore the regulatory mechanism after unilateral ureteral occlusion (UUO) .Methods UUO models are made by ligation unilateral ureteral with silk ,and they were divided into sham group and UUO group .All the mice were sacrificed by cer‐vical dislocation after 2 ,5 ,9 and 14 d of UUO .The kidney samples were examined for HIF‐1 mRNA ,VEGF mRNA and miRNA‐210 by quantitative real time reverse transcription polymerase chain reaction (RT‐PCR) ,and HIF‐1 protein by Western blot . Results The expression level of HIF‐1 mRNA was gradually raised after UUO (P<0 .05) ,and compared with the sham group ,the expression level of VEGF mRNA and miRNA‐210 were up regulated after 2 d of UUO and down regulated after 5 ,9 and 14 d of UUO compared with the sham group (P<0 .05);the expression level of HIF‐1 protein increased after UUO compared to the sham group .Conclusion The results of the level of HIF‐1 protein increased and VEGF mRNA and miRNA‐210 were up regulated ,which might be related to the adaptive response of kidney hypoxia and ischemia after UUO .
8.Biological characteristics of adipose-derived stem cells derived from renal fat capsule and groin in vitro
Yongsheng ZHU ; Qingfu DENG ; Jun LI
Chinese Journal of Tissue Engineering Research 2016;20(41):6197-6202
BACKGROUND:Adipose-derived stem cel s have different sources, but it is unclear whether these cel s from different sources have difference in their biological properties.
OBJECTIVE:To detect the in vitro proliferation and chondrogenic differentiation of adipose-derived stem cel s derived from renal fat capsule and groin.
METHODS:Adipose-derived stem cel s from renal fat capsule and groin of rats were isolated, cultured and identified. MTT assay was used to detect in vitro proliferation ability of these cel s. Passage 3 cel s were under chondrogenic induction for 2 weeks. After induction, the expression of type II col agen was observed by immunofluorescence detection, and RT-PCR was employed to detect the expression levels of Aggrecan and type II col agen mRNA in the two groups.
RESULTS AND CONCLUSION:After primary culture and passage, adipose-derived stem cel s from the renal fat capsule and groin of rats exhibited similar morphology, and over 95%of cel s expressed CD44 in the two groups. Adipose-derived stem cel s from two sources showed an S-shaped growth curve in vitro and were positive for type II col agen. After RT-PCR detection, the expression levels of Aggrecan and type II col agen mRNA had no difference in adipose-derived stem cel s from renal fat capsule and groin (P>0.05). Experimental results show that adipose-derived stem cel s from both renal fat capsule and groin exhibit stable growth, rapid proliferation and chondrogenic differentiation under orient induction in vitro, indicating there is no difference between these cel s from two sources.
9.Study on Syndrome Development Rules before and after Treating Primary Nephrotic Syndrome with Hormone
Jun WANG ; Xu DENG ; Meichang XU
Journal of Zhejiang Chinese Medical University 2016;40(9):669-676
Objective]Based on 346 cases of PNS patients using glucocorticoids observed during treatment, the main symptoms, syndrome differentiation of syndrome type of traditional Chinese medicine(TCM) by induction, and discusses its the evolution regularity of TCM Syndromes.[Methods]Develop a unified observation table, then clinicians recorded symptoms, signs, tongue picture and type of pulse to log on to observe truthfully and un-mistakbly table, after the end of observation, comprehensive analysis by the four examination methods, using a unified standards for TCM syndrome type determination. Discussion after glucocorticoids treatment of patients with PNS TCM syndrome objective laws of evolution.[Result]First ,TCM syndrome evolution:the results show that:before hormone treatment, Qi deficiency of spleen and kidney and Yang deficiency of spleen and kidney have become the most important type of syndrome;hormone use initial stages 1~4 weeks, Qi deficiency of spleen and kidney, deficiency of Qi and Yin, when the hormone used for 5~8 weeks. Yin deficiency of liver and kidney and deficiency of Qi and Yin occupied the main position; during the hormone withdrawal stage, the deficiency of Qi and Yin increased significantly; in the hormone maintain stage it was mostly deficiency of Yin and Yang and Yang deficiency of spleen and kidney. As for demonstration, in hormone therapy before a lot of patients suffering from retention of water and turbid damp;After the treatment of 1~4 weeks it was mostly humid heat ,turbid damp and blood stasis;the accompanying symptoms were mostly humid heat, blood stasis and pathogenic factor of wind after the treatment of 5-8 weeks ;Miscellaneous accompanying symptoms were very conspicuous except retention of water in the treatment of hormone withdrawal stage; In the hormone maintaining stage, the highest proportion of blood stasis compared with others. The use of hormone therapy, humid heat and blood stasis were more prominent than others. Second, after the hormone treatment(1~8weeks) the relationship between efficacy and TCM syndromes: A total of 130 patients enrolled, the 96 patients with Yin deficiency syndrome, the rest of the patients had no deficiency of Yin syndrome. The results indicated that Yin deficiency syndrome group serum albumin and 24-hour urinary protein excretion efficacy than those without Yin deficiency syndrome group, with statistical significance.[Conclusion] Glucocorticoids should belong to pure Yang medicine in traditional Chinese medicine, the body under the action of pure Yang drugs are prone to Yin deficiency symptoms, suggests that the higher the sensitivity to drugs, the clinical remission rate is also higher. This can be applied in clinical glucocorticoids and observe the change of the patients with syndrome, early in the patients' sensitivity to the glucocorticoids.
10.Advances in differential roles of HIF-1αand HIF-2αin the pathogenesis of hypoxic pulmonary hypertension
Jun DENG ; Xu ZHANG ; Shanshan YU
Chinese Pharmacological Bulletin 2017;33(1):10-13
Hypoxia-induced factors(HIFs)are the main regula-tors for the response of hypoxic environment.They are involved in hypoxia-related lung tissue cell damage and abnormal cell pro-liferation,among which,HIF-1αand HIF-2αplay the most im-prominant roles.This paper reviews the current researches of HIF-1αand HIF-2α,focusing on their structural and functional similarities and diversities,as well as their roles in the patho-genesis of hypoxic pulmonary hypertension.