STAT3, as a member of signal transducers and activators of transcription (STATs) family,which has been recognized as one of the common pathways in cancer cells. STAT3 has been found in many tumor cells that are critical to cell survival, cell proliferation, invasion, angiogenesis and immune evasion.Thus, STAT3 signaling pathway may be a potential therapeutic target for tumor metastasis.

Osteopontin (OPN), a phospborylated and glycosylated protein,is a prominent matrices component of the extracellular matrix of mineralized tissues of bones and teeth, in which they can regulate the formation and growth of hydroxyapatite crystals and influence a variety of cell activities.Recent studies have reported that the expression of OPN is not only correlated with bone formation and bone resorption but also with tumor aggression and metastasis in several types of human cancer,and OPN is even considered to be an index for the diagnosis and prognosis of tumor.This article reviews OPN's role in lung cancer' s infiltration, metastasis and prognosis.

Objective To investigate the effect and significance of cinobufagin on the expression of survivin of HHUA cells in the endometrial carcinoma cultured in vitro. Methods HHUA cells of endometrial carcinoma were cultured in vitro. After being intervened by cinobufagin with different concentration, the expression of survivin was measured by RT-PCR and the cellular apoptosis was tested by flow cytometry. Results In the control group: mRNA expression of survivin of HHUA cells in endometrial carcinoma was (1.22±0.23), cellular apoptutic rate was (2.31± 0.98)%; cinobufagin of 0.25 mg/ml didn't affect the survivin and cellular apoptotic rate (q=2.89, P>0.05: q=3.12, P>0.05) ; after being exposed to einobufagin of 2.5 mg/ml, the expression of survivin was (0.73± 0.26), and cellular apoptotic rate was (26.50± 6.36) %, with all of these data being different from the control group significantly (q=5.68, P<0.05; q= 10.23, P<0.05) ; the actions of cinobufagin of 25 mg/ml on mRNA expression of survivin and apoptotic rate were similar to cinobufagin of 2.5 mg/ml (q=3.49, P>0.05; q=4.35, P>0.05) . Conclusion Cinobufagin of 2.5 mg/ml inhibit the mRNA expression of survivin and proliferation of HHUA cells in endometrial carcinoma.

Objective To investigate the correlation between the expression of P-SAPK/JNK and neuronal apoptosis in the striatum during permanent middle cerebral artery occlusion (pMCAO) in rats.Methods Fifty-four male Sprague-Dawley rats were randomly divided into sham operation group and pMCAO 1-,3-,6-,12-,and 24-hour groups (n =9 in each group).Apoptotic neurons in the striatum during cerebral ischemia were detected by TUNEL assay,the nuclear translocation of P-SAPK/JNK in the striatum by immunohistochemical staining expressions of P-SAPK/JNK protein by Western blot.Results The numbers TUNEL- and P-SAPK/JNK-positive cells in the striatum at 1 hour after pMCAO increased significantly (P =0.000 1),and reached the peak at 6 hours.The numbers of TUNEL-positive cells decreased at 12 hours,however,it still higher than the sham operation group (P =0.000 2).Western blot analysis showed that the expression of P-SAPK/JNK after pMCAO increased significantly,and the time-course change was in accord with the result of immunohistochemical staining Neuronal apoptosis in the striatum was significantly positively correlated with the expression of P-SAPK/ JNK (r =0.984,P =0.000 4).Conclusions Cerebral ischemia may induce neuronal apoptosis in the striatum through the activation of P-SAPK/JNK.

Intracranial atherosclerotic stenosis mostly occurs in Asians,blacks and Hispanicsis,which is the most important reason for the occurrence and recurrence of ischemic stroke.The current studies mainly concentrate on the aspect of the relationship between the intracranial atherosclerotic stenosis and the traditional risk factors.With the development of genetic technology,the relationship between the genetic factors and intracranial atherosclerotic stenosis has also received increasing attention.This article reviews the advances in research on the traditional risk factors for intraeranial atherosclerotic stenosis and genetic research.

Objective: To measure the convergence angles of teeth in different teeth positions and different denture designs.Methods:Mesio-distal widths and labio-lingual widths were measured on 226 teeth according to three points in a line on three surfaces (gingival, middle and incise) of each tooth.The distance between every point and the counterpart point in the adjacent surface was measured.Then convergence angles were calculated. Results:The means of the angles were 4.13°±0.77°,4.14°±0.76°, 4.46°±0.95°, 4.76°±0.84° on the first and second premolar, the first and second molar,respectively . There were significant differences between the premolars and molars(P<0.05).On the dentures with the designs of monocrown, triunit-bridge and long bridge the means of the angles were 3.78°±0.74°,4.69°±0.75° and 5.08°±0.85° respectively,the angle on the denture with the design of monocrown was smaller than that of bridge (P<0.05).Conclusion:The convergence angles are different according to different teeth positions and different denture designs, so they should be adjusted according to the clinical requirements.

Objective To investigate the effect of mifepristone on the expression of β-endorphin in the ectopic focus of adenomyosis.Methods The expression of β-endorphin was measured by the immunochemistry method.Results During proliferative phase and secretory phase,the β-endorphin of mild dysmenorrhea were higher than that of midrange dysmenorrhea(t=2.672,2.711,all P＜0.05),the β-endorphin of midrange dysmenorrhea was higher than that of severe dysmenorrhea(t=2.341,2.365,all P＜0.05),the difference was significant.After being cured by mifepfistone,the expression of β-endorphin in the ectopic focus of adenomyosis was higher than that of control group (t=2.478,2.356,all P＜0.05),still lower than that of normal endometrial tissue,(t=2.123,2.233,all P＜0.05),the difference was significant.Conclusion The expression of β-endorphin in the ectopic focus of adenomyosis decreased significandy accompanied with the aggravation of the dysmenorrhea,and the mifepfistone could upregulate the β-endorphin and relieve the dysmenorrhea.

The mechanisms underlying early neurological deterioration (END) after ischemic stroke are not clear.There are no reliable predictive factors and effective preventive measures for END.The glutamatemediated excitotoxicity plays a very important role in the cascade reaction of ischemic events.High level of glutamate in plasma is one of the important predictors for END.Studies have shown that the polymorphism in the promoter of the excitatory amino acid transporter-2 gene is a potential cause for individual susceptibility to END.Some therapeutic strategies of interrupting the glutamatergic pathways may be as the strategies of intervention END.

ObjectiveTo evaluate the effect of compound oxymatrine injection combination with chemotherapy in patients with advanced ovarian carcinoma.MethodsEighty-six patients with advanced ovarian carcinoma were divided by random digits table method into observation group(43 cases) receiving compound oxymatrine injection plus chemotherapy(paclitaxel plus carboplatin) and control group (43cases) treated with chemotherapy(p aclitaxel plus carboplatin) alone.The change of white blood cell,adverse reaction and effect of the patients were observed.ResultsThe white blood cell of observation group after treatment was obviously higher than that of control group [ (4.7 ± 1.4) × 109/L vs.(3.2 ± 1.6) × 109/L,P ＜ 0.05 ].The rates of nausea and vomiting,transaminase elevating and kidney damage of observation group were obviously lower than those of control group[ 16.3％ (7/43),14.0％ (6/43),20.9％ (9/43) vs.34.9％(15/43),32.6％ (14/43),44.2％ ( 19/43 ),P ＜ 0.05 ].The complete remission rate of observation group was significantly higher than that of control group[55.8％(24/43) vs.27.9％(12/43),P ＜ 0.05].Conclusion Addition of compound oxymatrine injection to chemotherapy for patients with advanced ovarian carcinoma can effectively protect patients against the decreasing of the white blood cell and attenuate the side effects brought by chemotherapy.

ObjectiveTo investigate the effect of humanized nursing in ovarian cancer patients to improve treatment compliance and negative emotional state. Methods120 cases of ovarian cancer patients from October 2009 to October 2010 in our hospital were chosen as the research object.They were divided into the control group and the observation group according to randomization methods with 60 cases in each group.The control group patients were taken routine care.The patients in the observation group were taken humanized nursing on the basis of routine care.The treatment compliances during treatment were compared.The anxiety,depression scores for the two groups of patients before and after treatment were compared.Based on the three indicators,the effect of humanized nursing in ovarian cancer patients to improve treatment compliance and negative emotional state was evaluated. ResultsThe treatment compliance during treatment for the two groups was compared,the full compliance of patients of the observation group was significantly higher than the control group.The difference between the two groups was statistically significant.The anxiety,depression scores before treatment for the two groups were compared.There was no significant difference between the two groups.The anxiety and depression scores showed significant improvement after the treatment,but improvement level of the observation group was significantly higher than the control group.The difference between the two groups was statistically significant. ConclusionsHumanized nursing can improve the treatment compliance and negative mood of ovarian cancer patients effectively,it is conducive to the rehabilitation of patients,and is worthy of clinical application.