1.Comparison of HRP-labeled Neurons in Living and Dead Mice with Light and Electron Microscopy
Journal of Third Military Medical University 1983;0(04):-
Crystal HRP was applied to the cut ends of the left infraorbital nerve and branches of the facial nerve of 34 adult mice. Then the animals were killed and perfused, and HRP solution was applied to the cut ends of the root of the right facial nerve and the right maxillary nerve. Frozen sections of the brain stem and the trigerminal ganglia were made and reacted with BDHC, TMB or DAB method for light and electrom microscopic examination.Under light microscopy, the neurons labeled when alive in the left trigerminal ganglion and the nucleus of the left facial nerve showed that more cell bodise but less nerve fibers were labeled. The reaction products with all the 3 methods in thess cells were granular. The neurons labeled after death in the right trigerminal ganglion and the nucleus of the right facial nerve showed that more fibers but less cell bodies were labeled. The reaction products in these cells were also granular with BDHC and TMB methods but diffuse with DAB method.Under electron microscopy, there were electron-dense positive products in all the cells labeled either when alive or after death. In those cells labeled when alive, the products were found in the round or oval secondary lysosomes or multive-sicular bodies, while in those labeled after death, they were mostly found on one side of vacuoles without membrane boundary. In the fibers labeled after death, many large or small positive granules were found in the axons and many minute positive granules in the myelin lamellae especially on their inner surface in contact with the axons.
2.Laparoscopy in Treatment of Post-laparoscopic Cholecystectomy Complications:Report of 21 Cases
Chinese Journal of Minimally Invasive Surgery 2005;0(07):-
Objective To investigate the value of laparoscopic treatment for post-laparoscopic cholecystectomy complications.Methods From October 1996 to October 2007,totally 754 patients suffering from gallbladder diseases underwent laparoscopic cholecystectomy in our hospital;21 of them developed postoperative complications and received laparoscopic intra-abdominal exploration.Results By laparoscopy,bile leakage was diagnosed in 11 cases,intra-abdominal bleeding in 6,intraperitoneal fluid in 2,duodenal bulb perforation in 1,and obstructive jaundice in 1.The patients who developed obstructive jaundice was converted to open surgery because of hepatic duct transection.The other 20 cases underwent laparoscopic treatment.All the patients were followed up for a mean of 6 years(1 to 11),during which none of them had biliary complications.Conclusion Laparoscopic treatment is a microinvasive,safe and effective method for post-laparoscopic cholecystectomy complications.
4.More attention should be paid to the understanding of gastroenteropancreatic neuroendocrine tumors.
Chinese Journal of Oncology 2012;34(2):158-160
Antibodies, Monoclonal, Humanized
;
therapeutic use
;
Antineoplastic Agents
;
therapeutic use
;
Antineoplastic Combined Chemotherapy Protocols
;
therapeutic use
;
Bevacizumab
;
Gastrointestinal Neoplasms
;
classification
;
epidemiology
;
therapy
;
Humans
;
Indoles
;
therapeutic use
;
Neuroendocrine Tumors
;
classification
;
epidemiology
;
therapy
;
Octreotide
;
therapeutic use
;
Pancreatic Neoplasms
;
classification
;
epidemiology
;
therapy
;
Peptides, Cyclic
;
therapeutic use
;
Pyrroles
;
therapeutic use
;
Sirolimus
;
analogs & derivatives
;
therapeutic use
;
Somatostatin
;
analogs & derivatives
;
therapeutic use
5.Toward more understanding of esophagogastric cancer.
Chinese Journal of Oncology 2011;33(11):801-803
Antibodies, Monoclonal, Humanized
;
therapeutic use
;
Antineoplastic Agents
;
therapeutic use
;
Chemoradiotherapy
;
Chemotherapy, Adjuvant
;
Esophageal Neoplasms
;
classification
;
pathology
;
surgery
;
therapy
;
Esophagogastric Junction
;
Humans
;
Stomach Neoplasms
;
classification
;
pathology
;
surgery
;
therapy
;
Trastuzumab
7.Difference of colon cancer and rectal cancer-from the view of an oncological physician.
Chinese Journal of Oncology 2010;32(5):321-323
Antineoplastic Combined Chemotherapy Protocols
;
therapeutic use
;
Chemotherapy, Adjuvant
;
Chromosomal Instability
;
Colonic Neoplasms
;
drug therapy
;
genetics
;
pathology
;
Deoxycytidine
;
analogs & derivatives
;
therapeutic use
;
Fluorouracil
;
administration & dosage
;
analogs & derivatives
;
therapeutic use
;
Humans
;
Leucovorin
;
administration & dosage
;
Microsatellite Instability
;
Neoadjuvant Therapy
;
Neoplasm Staging
;
Organoplatinum Compounds
;
administration & dosage
;
Prognosis
;
Rectal Neoplasms
;
drug therapy
;
genetics
;
pathology
;
Risk Factors
10.Raloxifene combined with calcium phosphate cement for repair of rabbit mandibular defects
Chinese Journal of Tissue Engineering Research 2014;(25):3993-3997
BACKGROUND:Raloxifene is the third generation of selective estrogen receptor modulators, which can decrease bone loss, increase bone mineral content, and reduce fracture risk. OBJECTIVE: To study the effects of raloxifene combined with self-setting calcium phosphate cement on the repair of rabbit mandibular defects. METHODS:Totaly 36 New Zealand white rabbits were selected to prepare 8 mm×4 mm×3 mm mandibular defect models, and then randomized equaly into experimental group (raloxifene, 7.5 mg/kg per day, combined with self-setting calcium phosphate cement), drug group (raloxifene, 7.5 mg/kg per day), artificial bone group (self-setting calcium phosphate cement). Rabbits were sacrificed 4, 8 and 12 weeks later, respectively, for measurement of bone morphogenetic protein 2 using immunohistochemistry method and transforming growth factor β using a laser scanning confocal microscope. RESULTS AND CONCLUSION:After 4 and 8 weeks, the expression of bone morphogenetic protein 2 was obviously higher in the experimental group than the drug and artificial bone groups; after 12 weeks, bone remodeling was basicaly complete in the experimental group, and the expression of bone morphogenetic protein 2 became lower than that in the other two groups. The expression of transforming growth factor β in the experimental group was gradualy increased and reached the peak at 8 weeks, while in the drug and artificial bone groups, the expression of transforming growth factor β exhibited an increasing trend within 4-12 weeks, which was close to the peak. These findings suggest that raloxifene can promote early expression of bone morphogenetic proteins and early calus formation as wel as accelerate the repair of bone defects with calcium phosphate cement.