ObjectiveTo investigate the effects of recombinant human erythropoietin (rhEPO) on the apoptosis of human mesenchymal stem cells induced by H2O2 and related cell signaling pathways.Methods After being cultured in vitro, human MSCs were treated with rhEPO.Phosphorylation of ERK1/2,p38 MAPK and PI3K/Akt was detected by using Western blotting.One h after pretreatment with1U/ml rhEPO,cells were cultured in the presence of1mmol/L H2O2 for1h.Cell morphology was observed under the inverted microscopy.Cell apoptosis was determined by using flow cytometry,migration assay was performed in Transwell chambers,and adhesion assay was performed by plastic dishes.ResultsRhEPO could increase phosphorylation of PI3K/Akt pathway in human MSCs,but reduce phosphorylation of p38MAPK.RhEPO had no obvious effect on ERK1/2 pathway and total proteins of Akt and p38MAPK.RhEPO could decrease apoptosis of human MSCs induced by H2O2 (P＜0.01) and the inhibitory effect was abrogated by Ly294002 but not anisomysin.Conclusion RhEPO could protect MSCs from apoptosis. Activation of PI3K/Akt pathway was involved in the effect of rhEPO on apoptosis.

OBJECTIVE To investigate the prevalence of metallo-?-lactamase in carbapenem-resistant clinical Pseudomonas aeruginosa isolates.METHODS Sixteen strains of P.aeruginosa isolated from Tianjin General Hospital during 2005-2006 which were resistant to all tested antibiotics besides carbapenem were studied.The general PCR was adopted to amplify the resistance genes of matallo-?-lactamae.Restriction endonucleases performed restriction analysis were used to certify the type of genes of matallo-?-lactamase by different endonuclease recognition site.RESULTS Two of the 16 clinical strains of P.aeruginosa(named P02 and P16) were confirmed bearing IMP-1 metallo-?-lactamase gene detected by PCR amplification and restriction analysis.CONCLUSIONS P.aeruginosa producing IMP-1 metallo-?-lactamase is the major type in our hospital.The hydrolyzing effect of the metallo-?-lactamase is not the main machanism in these carbapenem-resistant P.aeruginosa isolates.

Objective To determine the genotypes of human papillomavirus (HPV) and to estimate their recurrence of condyloma acuminatum (CA) among female in Xi'an.Methods HPV genotype was detected with human papilloma virus genotyping kit in 63 female patients with CA.Follow-up was carried out to observe the recurrence of CA in these patients after CO2 laser treatment.Results Of the 63 specimens,61 (96.83％) were positive for HPV,and a total of 16 HPV subtypes were identified.The predominant genotypes were HPV6 (59.2％),11 (22.95％),42 (8.20％),43 (8.20％),52 (8.20％),16 (6.56％) and 18 (4.92％) respectively.43 cases of single HPV infection was accounted for 70.49％ (43/61),mixed infection in 14 cases was accounted 22.95 ％ (14/61).61 cases of patients with laser treatment,observing the recurrence of cuses after 3-month later,it was found that the recurrence rate in higher risk group and mixed infection group was higher than that in low-risk group.Conclusion HPV6,11 is main HPV infection type of the female patients in Xi'an,the recurrence of CA is higher in patients with high risk HPV than in those with low risk HPV and mixted infection.Mixted infection of HPV and high risk HPV is a risk factor for recurrence of female genital warts in Xi'an region.

Dysarthria ; etiology ; Encephalitis, Viral ; complications ; Facial Paralysis ; etiology ; Humans ; Syndrome

Acupuncture Points ; Acupuncture Therapy ; instrumentation ; Adolescent ; Adult ; Female ; Humans ; Male ; Middle Aged ; Needles ; Urticaria ; therapy ; Young Adult

OBJECTIVETo investigate the reproducibility of Gleason scores for prostate cancer.

METHODSBased on the revised Gleason Scoring System of the International Society of Urological Pathology ( ISUP) , we analyzed the reproducibility and difference of Gleason scores in 49 cases of prostate cancer using the methods of combination and grouping.

RESULTSThe total reproducibility of Gleason scores among 15 pathologists was good (κ = 0.642), 62.2% by the combination method, the highest in Gleason 5 + 5 (81.2%) and 5 +4 (73.3%), then in Gleason 4 + 4 (67.5%), 3 + 3 (64.0%), 4 +3 (61.3%), and 3 + 4 (44.0%), and the lowest in Gleason 4 + 5 (38.9%) and 3 + 5 (33.3%). The total reproducibility of Gleason scores by the grouping method was 71.4%, the highest in Gleason 9-10 (84.9%) , then in Gleason 7 (76.7%) and 6 (64.0%), and the lowest in Gleason 8 (60.7%).

CONCLUSIONThe reproducibility of Gleason scores remains to be further improved in prostate cancer, mainly concerning the understanding of Gleason 3 and 4 carcinoma.

Non-alcoholic fatty liver disease (NAFLD) is an important type in chronic liver diseases. Its incidence is growing. It is regarded as the liver manifestation of metabolic syndrome, which is closely correlated with the onset of cardiocerehrovascular diseases. The prevention of stroke in patients with NAFLD is very important. Statius are the most important type of medications. They decrease cholesterol synthesis, upregulate low-density lipoprotein (LDL) receptor in the liver and lower the levels of low-density lipoprotein cholesterol (LDL-C) in circulation through inhibiting hydroxy-methyl-glutaryl coenzyme A (HMG-CoA) reductase, and thus effectively reduce the risks of stroke. Besides, the pleiotropy of statins and their effects on cholesterol-related cell signaling pathway may relieve or prevent the progression of NAFLD. There is greater controversy about whether statins can be used in patients with chronic liver diseases because they have some adverse reactions on liver, The evidence available has demonstrated that statins can he used safely in patients with NAFLD, and usually liver enzymes have no need to be monitored. Too much attention on the liver toxicity of statins may result in inappropriate drug withdrawal, and brings about the increased risk of cardiovascular events. So further evaluation is needed for the efficacy and safety of statins in patients with NAFLD.

After the first recognition occurs between the activated sperm and zona pellucida of the oocyte from the mammalians, ?-1,4-galactosyltransferase I (? 4GalT I) combines the N-GlcNAc terminals by O-ligands on the ZP3 of the zona pellucida, which plays a difunctional role in the fertilization. The G protein signal system on the sperm membrane then is consequently activated by ZP3 via ?4GalT I, contributing to the induction of acrosome reaction. It was proved that in the activation of the G protein system, both the BBXB and BBXXB motifs on the N terminal of the long ? 4GalT I are necessary.

Objective To study MRI characteristics of viral encephalitis and the possibility of differentiating diagnosis with acute disseminated encephalomyelitis(ADEM).Methods MRI findings in 56 patients with viral encephalitis and 50 patients with ADEM were analysed and emphasized on MR findings of viral encephalitis.Results The abnormal signal lesions were found in 56 patients with viral encephalitis,including multiple or single,symmetrical or asymmetrical large patch shape lesions,the lesions located mainly in cortices,subcortical and basal ganglia and thalami.The lesions appeared as long T_1 and long T_2 on MRI.Enhanced MRI was performed in 27 cases,and demonstrated abnormal enhancement in 17 cases,including large patch or gyrus shape enhancement in 10 cases.Multiple and asymmetrical spot-patch white matter lesions were revealed in the frontal lobe,parietal lobe,occipital lobe,temporal lobe,and periventricular areas with ADEM.The lesions were long T_1 and long T_2 on MRI.Enhanced MRI was performed in 20 cases,and demonstrated abnormal enhancement in 15 cases,peripheral ring-type or patch shape enhancement was observed in all cases.Conclusion MRI is one of the important methods in diagnosis of viral encephalitis,it can provide evidences for early diagnosis and differentiation in the certain degree.