Objective To observe the effect of SIRT1 on the proliferation of ovarian cancer CAOV3 cells and its possible mechanism. Methods Ovarian cancer CAOV3 cells were transfected the Lipofectamine 2000 and were divided into normal control group,negative control group and SIRT1-siRNA group.The mRNA and protein expression levels of SIRT1 were detected by RT-PCR and Western blot.MTT method was used to detect the proliferation of RNA interference at 24h,48h and 72h,respectively.The distribution of CAOV3 cell cycle phase after RNA interference of SIRT1 for 72 hours was detected by flow cytometry.SIRT1,p53,p21 protein expression and p53 acetylation status were detected by Western blot with RNA interference of SIRT1 after 72 hours.The interaction between SIRT1 and p53 protein was verified by immunoprecipitation in CAOV3 cells.Results Compared with negative control group,the expression of mRNA and protein of SIRT1 in SIRT1-siRNA group decreased after transfection of SIRT1 for 72 hours.The survival rate of CAOV3 cells in SIRT1-siRNA group decreased significantly at 48 hours and 72 hours (P<0.01).The proportion of Sphase cells in CAOV3 cells transfected with SIRT1-siRNA for 72h was significantly decreased (P<0.01),while the G1 phase cells were significantly increased (P<0.01),and the cells arrested in the G1 phase.The expression of p53 and p21 proteins were significantly increased and the p53 was in higher acetylation status after transfection with SIRT1-siRNA for 72 hours ( P<0.01 ) .SIRT1 was interacted with p53 protein in CAOV3 cells confirmed by co-immunoprecipitation. Conclusion Downregulation of SIRT1 expression can inhibit the proliferation of ovarian cancer CAOV3 cells, which maybe connected with p53 at high acetylation status,and enhancing p53-dependent p21 transcriptional activation,thus blocking the cell cycle and inhibiting the cell proliferation.

Objective To summarize the clinical data of patients with diabetic ketoacidosis (DKA), and improve the ability of diagnosis and treatment for patients with DKA. Methods The clinical data of 224 patients with DKA were retrospectively analyzed. Results After the positive diagnosis and comprehensive treatment including liquid-supplementing and small doses of insulin therapy, in 224 patients, 210 cases were cured, and the treatment success rate was 93.75%. Fourteen cases were dead, and the fatality rate was 6.25%. Conclusions Early diagnosis, liquid-supplementing and reasonable application of insulin therapy is the key to the success of DKA treatment.

Objective To observe the induction of tolerogenic dendritic cells (DCs) by curcumin (Cur) and its mechanism.Methods After immature DCs from bone marrow cells of Wistar rats were treated with different concentrations of Cur (0,10,20 and 30?mol/L) respectively,and then the DCs were tested by flow cytometry for the surface molecules expression.After the immature DCs were treated by 30?mol/L Cur with or without stimulation of LPS,endocytosis of DCs to dextran was tested by flow cytometry.The production of IL-12 in DC culture supernatant was determined by ELISA.The levels of NF-?B p65 and RelB translocation to the nucleus were investigated by Western- blot.The activity of NF-?B was detected by NF-?B-binding ELISA and luciferase reporter gene analy- sis.The ability of DCs to stimulate the proliferation of T cells from Lewis rats were analyzed by mixed leukocyte reactions (MLR).Results Cur suppressed LPS-indueed cell-surface expression of costimu- latory molecules (CD80,CD86 and CD40) in a dose-dependent manner.When Cur was used at a con- centration of 30?mol/L,there was no marked difference in the surface molecules expression of LPS- inducing DCs as compared with immature DCs.After DCs were induced by LPS (LPS group),the positive rate of FITC-Dextran uptake was (36.6?7.2)%,and the secretory amounts of IL-12 were (415.9?42.7) pg/ml.In DCs of LPS group,the intranuclear RelB and p65 were highly expressed and their DNA binding activity was 0.65?0.08 and 0.74?0.07 respectively.The luciferase activity of reporter gene in LPS group DCs was remarkably increased to 435% as compared with that in the controls.DCs in LPS group showed strong capacity to stimulate T cells proliferation.When DCs were treated with 30?mol/L Cur followed by induction with LPS (Cur+LPS group),the positive rate of Dextran uptake was (78.6?14.2)% and remarkably higher than in LPS group (P

Objective To investigate the effect of hypoxia on vascular endothelial growth factor (VEGF) expression and secretion in human renal fibroblasts. Methods Human renal fibroblasts were primary cultured from donor kidney, and cells in passage 3 were used in this experiment. Hypoxia was induced by incubation in a gas-tight box gassed with 10% CO2/10% H2/80% N2 for 24 hours. VEGF mRNA levels were measured by semi-quantitative RT-PCR method. VEGF proteins expressed on cell surface were measured by flow cytometry. VEGF proteins secreted into supernatants were measured by ELISA method. Results Hypoxia caused a 2. 5-fold increase of VEGF mRNA level in human renal fibroblasts. Hypoxia also induced significant increases of VEGF proteins on cell surfaces (mean fluorescence intensities: 1. 053?0. 055 vs 0.763?0.057, n=4,P

Alkaloid is one of the main antineoplastic activities in Chinese Herbal Medicine.There has been big progress on the mechanism of antitumor in natural alkaloids and their derivatives in recent years,especially focusing on the targets of hypoxia inducible factor 1,telomere,and topoisomerase.The new trends of these three targets have been reviewed in this paper.

Objective To study the effect of temperature on the emission of tbrmaldehyde trom the blockboard.Methods A 0.25 m3 simulation chamber was used to explore the emission of formaldehyde from blockboard at the temperature of 15,17,19,21, 23,25,27,and 29 ℃,respectively.The relative humidity,the ventilation velocity and the loading factor in simulation chamber was (50?1)%,1.0 L/min,and(1?0.03)m~2/m~3 respectively.Results A hyperbolic logarithm variation in formaldehyde concentration with time in the chamber was observed at different temperatures.Formaldehyde concentration in the chamber increased remarkably once the temperature exceeded 25 C.The chamber stabilization formaldehyde concentration varied with temperature in accordance with an exponent equation of y=0.921 9?0.024 9e~(0.176 4x)(R~2=0.996 9).Conclusion The temperature can greatly influence formaldehyde emission from the blockboards.Higher temperature will facilitate formaldehyde emission from the blockboards.

Objective To study the effects of rehabilitation training on the regeneration of nerve cells in rats after intracerebral hemorrhage (ICH).Methods A total of 75 male SD rats were randomized into a training group,a control group and a sham operated group,25 rats/group.The ICH models were induced by stereotactical injection of collagenase type Ⅶ into the globus pallidus.The training group was trained with grasp,balancing and rotating exercise every day,the control group was restricted to their cages,and the sham operated group received normal saline injections.Each group was further subdivided into 1,4,7,14 and 28 day subgroups.Neurological function was measured in each group.Bromodeoxyuridine (BrdU) was used to label S phase cells,immunohistochemical single and double staining with antibodies against BrdU,microtubal-associated protein (MAP) and neuronal nuclei (NeuN) were used to determine neuronal proliferation,migration and differentiation in the subventricular zone ( SVZ ) and subgranular zone (SGZ) in the training and control groups.Results The motor function scores of the animals in the rehabilitation group were significantly lower than those of the control group.Proliferative BrdU + cells of the SVZ and SGZ in the control group rats were clearly less than those in the rehabilitation training rats at all time points.The results of the immunohistochemical double staining indicated that one week after ICH BrdU +/MAP + cells in the SVZ had increased significantly in the training group compared to the control group,and then decreased two weeks later.At the same time,BrdU +/MAP cells were found in the striatal boundary on the hemorrhage side,in numbers up to 8 times that in the control group.In the rehabilitation group striatal neuron differentiation on the hemorrhage side was 2 to 3 times that in the control group.Conclusion Rehabilitative training can enhance nerve cell proliferation,regeneration and neuron migration after ICH.

Objective To investigate the best dignostic cut-off point of non-typical ectopic pregnancy(EP) through the determination of vascular endothelial growth factor(VEGF)in 154 cases of suspected ectopic pregnancy patients.Methods The serum level of VEGF in 154 cases with suspected EP was determined by means of immunossay technique,and the results were made into ROC-Curve.Results The acreage behind the curve was 0.95,illustrating that the VEGF can be as a better marker for early diagnosis of EP.The serum VEGF level of 200pg/ml was the cut-off point for early diagnosis of EP throash ROC-Curve.Condusion The serum VEGF can be considered as an early diagnostic marker of EP,which cut-off point is benifical to reducing misdiagnosis of EP.

During the period of establishing and perfecting socialist market economy in current situation,the professional morality of medical trade emerges much more new matters and problems and faces severe challenges.This article analyses the causes of these problems and difficulties on improving the professional morality of medical trade nowudays,and raises some strategies to solve these problems,such as reforming the medical system in-depth,strengthening supervision and so on.

OBJECTIVE:To introduce coordinate localization method as a new pharmaceutical care method to provide reference for rational use of drug in cancer patients. METHODS:The way that clinical pharmacists assisted physicians in prescribing for cancer patients with coordinate localization method was interpreted combining with examples. RESULTS:Coordinate localization method was conducive to rational use of drug in cancer patients and pharmacist’s self- quality construction. CONCLUSION:Coordinate localization method can effectively assist the doctor to prescribe rationally.