Oral imaging techniques have been widely and increasingly used in clinical practice.This article reviews the functions,characteristics and principles of three kinds of oral imaging instruments including panoramic x-ray unit,intraoral x-ray unit and stomatology endoscope.

It is considered that the central nervous system (CNS) is an immunologically privileged organ, but its immunological privilege is not complete, and immunological rejection may also occurred after tissue transplantation. Neural stem cell (NSC) transplantation has developed a brand-new approach for the treatment of various CNS diseases. Despite the low immunogenicity of NSC, there are also troubles of immunological rejection. This article reviews the immunological characteristics of CNS, the mechanisms of immunological response and immunological rejection in CNS, as well as the problems of immunological rejection of NSC transplantation.

Objective To study the shape differences of upper epidermal cells in leaves of medicinal plants of Swertia L. and establish the new method for identifying them. Methods The epidermis were mounted in ordinary technique, and then scanned and binarized through HPIAS-1000 image analytic system. The waveness of the anticlinal walls (SFC) and the ratio of the Ferets diameter (SLF) of upper epidermal cells were detected. The leaf epidermal cells of 21 kinds of raw materials in 12 plants of Swertia L. from various regions were examined. Results The precision and reproducibility of software system were good, and the shape characters of the upper epidermal cells of the middle lamina in the third node arising from ground are the stablest inside the same species by the magnification 20?10 under microscope. The SFC and SLF of the upper epidermal cells of the same species of plants are relatively constant, conversely that of different species of plants are obviously different and distinguishable from each other. Conclusion The proposed method is a useful technique for identifying traditional Chinese medicinal materials originated from herbs and leaves of plants. HPIAS-1000 is simple, rapid, accurate, and practical for shape analysis of upper epidermal cells of plant leaf.

To prepare large naive phage antibody library, the host bacteria with high transformation efficiency is used in the Cre-LoxP recombination system. The variable regions of immunoglobulin light and heavy genes were amplified from lymphocytes collected from adult peripheral blood and newborn cord blood. The genes were spliced to form the single-chain variable fragments (scFv) by overlap PCR, cloned into pDAN5a vector and then transformed into XL2-blue MRF' with the Hte gene. Compared with XL1-blue strain, the size of the primary library was increased by 3.9 times. The primary library infected Cre recombinase-expressing bacteria, and the genes between phagemids created many new VH/VL combinations. The library was calculated to have a diversity of 1.7 x 10(11) and validated by the selection of antibodies against six different protein antigens. This library provides the basis for further selection of antibody-based drugs. It is the first time to report that XL2-blue MRF' can be used to improve the diversity of the library in the recombination system.

With the development of therapeutic antibodies over the past decade, they have become the treatment options for immunity and inflammation diseases. Major limitations of mouse antibodies as therapeutic agents - immunogenicity, lack of effectors' functions and short serum half-life -- were subsequently identified and largely overcome by the advent of humanized and fully human antibody technologies. The therapeutic antibodies for immunity and inflammatory diseases are primarily utilized in the treatment of allograft rejection, autoimmune disease, autoinflammatory syndromes, allergies and other chronic inflammation. The action mechanisms of therapeutic antibody include blocking ligands or receptors, regulating receptor activity, clearing the target cells or activating receptor. Strategies for generating the antibody drugs with high efficacy and low side effects can be realized by modulation of Fc-mediated activities and optimization of antigen-binding domains.

Objective To evaluate the preventive effects of dezocine on postoperative hyperalgesia in patients after remifentanil-based anesthesia.Methods One hundred ASA Ⅰ or Ⅱ patients,aged 20-64,weighing 45-65 kg,undergoing laparoscopic cholecystectomy,were randomly divided into 4 groups ( n =25 each): fentanyl group (group F),low-dose dezocine group (group D1 ),medium-dose dezocine group (group D2 ) and high-dose dezocine group (group D3 ).Anesthesia was induced with midazolam,propofol,remifentanil and cisatracurium.The patients were mechanically ventilated after tracheal intubation.Anesthesia was maintained with target-controlled infusion of remifentanil (target effect-site concentration 4 μg/L), intravenous infusion of propofol 4-6mg· kg-1· h-1 and intermittent intravenous injection of cisatracurium 0.03 mg/kg.Groups D1,D2 and D3 received intramuscular injection of dezocine 0.1,0.2,0.3 mg/kg at 30 min before the end of surgery respectively and group F received intravenous injection of fentanyl 1 μg/kg at 15 min before the end of surgery.The emergence time and extubation time were recorded.Visual analog scale(VAS),Bruggrmann comfort scale(BCS) and sedation and agitation scale (SAS) at immediately,1 h,2 h after emergence from anesthesia (T0,1,2),and the incidence of respiratory depression,nausea and vomiting and urinary retention after extubation were recorded.Results Compared with group F,the emergence time and extubation time were shortened,the incidence of respiratory depression and nausea and vomiting was decreased in groups D1,D2 and D3,VAS score was increased and BCS score was decreased at T0-2 in group D1,VAS score at T0 was increased,while VAS score at T1,2 and SAS score at T0-2 was decreased in groups D2 and D3 ( P ＜ 0.05).Compared with group D2,SAS score was decreased at T0-2,while the incidence of respiratory depression was increased in group D3 ( P ＜ 0.05 ).No patient showed urinary retention in the four groups.Conclusion Intramuscular injection of dezocine 0.2 mg/kg at 30 min before the end of surgery can reduce postoperative hyperalgesia with little side effects in patients after remifentanil-based anesthesia.

To investigate the effects of Yiniao Recipe, a compound traditional Chinese herbal medicine, on contents of serum antidiuretic hormone, and plasma cyclic adenosine monophosphate and cyclic guanosine monophosphate in rats with kidney-yang deficiency.

Objective To learn the emergency response capacity of health authorities at city level in Shandong province in order to discover loopholes and make improvement. Methods The health emergency questionnaire designed by China Health and Family Planning Commission was used for survey of 1 7 city-level health authorities in Shandong province.Results 52.94% of the health authorities were found with health emergency reserve funding; 100.00% of them with public health emergency contingency plans;94.12% of them having health emergency supplies in kind;100.00% of them having health emergency training for all of the staff;100.00% of them with direct web reporting for public health emergency.Conclusion Such authorities were found with satisfactory health emergency capacity in general.Defects were found with emergency funding shortage,poor training and drilling outcomes, poor material reserves management,and insufficient health emergency personnel.

This study aimed to summarize the diagnosis and treatment of uncommon complications in esophageal and cardiac cancer operations as well as obtain lessons from the failure. Methods:The etiology, clinical diagnosis, treatment, prevention measures, and factors that contribute to the failure of the uncommon complications were analyzed retrospectively. Results: Results showed ten cured cases and eight death cases (except for the reported eight cases). Conclusion:The following conclusions were ob-tained. 1) The complications described in this paper were rare, but they sometimes occur clinically. If wrong treatment was taken, seri-ous consequences would be expected. 2) Once a jet-like bleeding of aortic esophagus avulsion injury occurs, the surgeon must be calm and take proper treatment to successfully patch up the wounds. 3) The right thoracic approach is a new approach to prevent the injury of azygos vein. 4) Patients with anastomotic stoma fistula easily cause a delay in diagnosis if the leakage was wrapped in the inferior phrenic. 5) Taking preventive measures is the key method for the anastomotic aortoesophageal fistula. 6) Occurrence of pulmonary em-bolism after operation should be monitored. 7) Thoracic gastric mediastinal hernia would cause serious consequences if treatment was not taken at a proper time.