砄bjective:To study the effects of superlong pedicled latissimus dorsi muscle transplantation (SPLDMT) in the treatment of facial paralysis.Method: 16 cases of unilateral long standing facial paralysis were treated with SPLDMT in 1991～1998;follow up was conducted and the effects were analysed. Results:After operation satisfied movement recovery of the facial muscles was observed in 12 and improvenent in 3 of the 16 patients,no change was found in only 1 case. Conclusion: SPLDMT is effective in the treatment of unilateral long standing facial paralysis.

Background and purpose:How to predict the survival length for terminally cancer patients is very important, it will help families and physicians to make decisions. This study aimed to reveal the factors related to the survival time of terminally ill cancer patients who received palliative care in our hospital. Methods:The clinical data of 271 dead patients treated in the Department of Palliative Care, Fudan University Shanghai Cancer Center from Mar. 2007 to Mar. 2012 were analyzed. The Kaplan-Meier method and the log-rank test were used to determine the corresponding factors with survival. Cox regression model was used to examine the independent prognostic factors. Different survival length of groups divided by different prognostic indexes was compared by log-rank test. Results:Seven factors were found to be related with the survival according to univariate analysis. The related factors were Karnofsky performance score (P<0.001), dyspnea (P=0.037), delirium (P=0.015), high white blood cell count (P=0.012), low lymphocyte percentage (P=0.030), high lactate dehydrogenase (P<0.001) and low serum albumin (P=0.001). The multivariate analysis selected four independent factors:Karnofsky performance score<30, high lactate dehydrogenase, low serum albumin and delirium.Conclusion:The study shows the clinical survival prognostics with Chinese characteristics. The combination of the seven factors may be useful but more studies in this area deserve further investigated.

Objective To compare the orthodontic efficacy of the self-locking appliance and the ordinary straight wire appliance.Methods A total of 144 patients from March 2010 to March 2012 at the Department of Stomatology Orthodontic Surgery,Zhejiang Dongyang City People's Hospital were divided into observation group (n =72) and control group (n =72) according to the random number table.The observation group were treated with the self-locking appliance,and the control group were treated with the ordinary straight wire appliance.Results (1) The number of referral unconventional visits had no statistically significant difference between two groups (U =-1.65,P =0.0990).The number of routine visits of observation group was significantly less than that of the control group (U =-2.80,P =0.0052).(2) The VAS score of observation group was significantly lower than that of the control group (U =-8.47,P =0.0000).Conclusions The orthodontic efficacy of the self-locking appliance is obviously better than the ordinary straight wire appliance,and the former-caused pain is lighter.Therefore,the self-locking appliance is more suitable for orthodontic surgery.

Colorectal Neoplasms ; pathology ; Humans ; Neoplasm Staging

Adaptor Proteins, Signal Transducing ; chemistry ; metabolism ; Animals ; Apoptosis ; Cell Differentiation ; Cell Line, Tumor ; Cell Proliferation ; Humans ; Neoplasms ; pathology ; Phosphorylation ; Protein Isoforms ; RNA-Binding Proteins ; chemistry ; metabolism ; Transcription Factors ; antagonists & inhibitors

Objective: To study the influence of inhibitor of differentiation 2 (Id2) on cell proliferation and migration in SKOV3 cells, and to investigate the influence of HLH domain deletion in 1d2 gene on SKOV3 cells. Methods: Human ovarian cancer cell line SKOV3 was transfected with pcDNA3.1-Id2, pcDNA3. 1-Id2-DBM and pcDNA3. 1-Id2-DBM-δHLH vectors by SuperFect Transfection Reagent. The cells transfected with blank vector pCDNA3. 1 were used as control. Id2 protein was detected by Western blot, and mRNA transcriptions of Id2, Id2-DBM (D-box mutant), and Id2-DBM-δHLH were measured by RT-PCR. Cell proliferation was assessed by MTT assay. Cell invasion was detected by scratch test and transwell chamber assay. The regulatory effect of Id2 on E-cadherin was evaluated by Western blot. Results: The mRNA and protein levels indicated that the plasmid was successfully transfected. Cell growth curve suggested that the proliferation of cells had no significant difference between each group. Compared with the control group, cell migration ability was significantly enhanced after transfection with pcDNA3. 1-Id2, pcDNA3. 1-Id2-DBM and pcDNA3. 1-Id2-DBM-δHLH vectors. The downregulation of E-cadherin protein was accompanied with increased migration capability in pcDNA3. 1-Id2-DBM and pcDNA3. 1-Id2-DBM-δHLH vectors-transfected cells. Conclusion: Overexpression of Id2 promotes the migration capability of SKOV3 cells, which might be related with the downregulation of E-cadherin. The action still exists after the HLH domain deletion in Id2 gene.

Objective To explore the influence of earlier nasal continuous positive airway pressure(NCPAP) applying on pulmonary function,and evaluate the clinical results of the early application of NCPAP.Methods Ninety newborn infants with pulmonary dynspnea treated with face mask from Jan.2000 to Nov.2002 were selected as control group.One hundred and ten patients treated with NCPAP from Dec.2002 to Dec.2006 were selected as treatment group.Treatment group applied KD-300 CPAP oxygen therapy equipment NCPAP delivering oxygen.Setting up and adjusting parameter: the beginning flow volume 6-8 L/min,the oxygen therapy bulk(FiO2)30%-50%,the NCPAP pressure was 0.195-0.488 kPa,parameter was adjusted according to the sickness and detecting the transcutancous oxygen saturation(TcSO2).If nasal cannulae Oxygen therapy couldn′t keep the TcSO2 in 85%-93%,the patients in control group were used face mask,small face mask,the oxygen flow vo-lume was 6 L/min,three holes,average FiO2 was 59%.The artery blood oxygen partial pressure [pa(O2)],artery blood carbon dioxide partial pressure [pa(CO2)],oxygenation index(OI),respiration rate(RR) of 2 groups after therapy 4,24 and 72 h were observed,also the difference of recovery rate between the 2 groups were analyzed.Results Compared with the control group,pa(O2) and OI after the application of NCPAP in treatment group were obviously improved(Pa

Monocytes,Kupffer cells,spleen macrophages,lung and peritoneal macrophageswere isolated from same rat simultaneously.The effector cells from diverse anato-mical sites were checked for their tumor cytostatic properties using an in vitro tar-get cell growth inhibition assay through measuring (~3H)-TdR incorporation intotumor cell DNA.All of them expressed spontaneous cytostatic activity against hu- man SMMC-7721 hepatoma and mouse YAC-1 lymphoma cells.The effector cells,except monocytes,were shown to exert inhibition on various target cells with diffe-rences in their susceptibility.The cytostasis against either tumor cell lines variedwith five types of effector cells.The most effective cells for inhibiting the growthof lymphoma were monocytes,and Kupffer cells for heptoma,The effector cellsstimulated by Corynebacterium parvum (CP) in vitro were shown that their cytostaticactivity against YAC-1 was increased up to 140-408%,but to heptoma,it wasdecreased to 48-64%.Furthermore,the efficiency of CP stimulation varied withdifferent effector cells also.It is concluded that monocytes and macrophages withsuch natural selective tumor cytostatic capacity were thus everywhere readily availa-ble for activation.CP may enhance or inhibit the cytostatic effect of monocytes andmacrophages on diverse tumor cell lines.It might suggest that the use of CP in thetumor immunotherapy should be considered with different type of tumors.

Objective For the purpose of exploring the influence exerued by the amount of template DNA on the results of automatic fluerescent detection. Method For the purpose of buiding the optimal amount of template DNA amplified, we have analyzed different amount of template DNA (9947A) by 3100 Genetic Analyzer with the multiplexing Profiler Plus Kit. Results The optimal amounts of template DNA amplified were between 0.31ng and 2. 5ng by using 3100 Genetic Analyzer. Conclusion It will produce the incorrect analytic results if the amounts of template DNA were too high or too low.

Heart Aneurysm ; mortality ; pathology ; surgery ; Humans ; Mitral Valve Insufficiency ; mortality ; physiopathology ; surgery ; Myocardial Infarction ; mortality ; physiopathology ; surgery ; Ventricular Dysfunction, Left ; pathology ; surgery