Whether resection for colorectal cancer could achieve the same effect as open surgery had been doubted. Recently, the international large prospective random control tests and large retrospective studies have revealed that laparoscopic resection for colorectal cancer not only has short-term advantages such as minor injuries, less postoperative pain, quicker recovery of bowel function and less postoperative hospital stay, but also had at least the equivalent long-term effects in local recurrence, distant metastasis, incision implantation and long term survival. It is promising that the roll of laparoscopic surgery for colorectal cancer will be defined by more evidence-based medicine study in the near future.

It is widely accepted that diabetes is intimately linked to diet. The latest researches showed that diet-microbiota interactions acted as moderators of human diabetes. Gluten-free diet and hydrolyzed protein decreased the incidence of type 1 diabetes mellitus (T1DM)by the mechanism of changes in gut microbiota and immune micro-environment. Consuming more dairy products decreased the incidence of type 2 diabetes mellitus (T2DM). High fat diet can induce insulin resistance (IR),thus increasing the incidence of T2DM. Parental dietary affected on offspring diabetes by the epigenetic mechanism. Low carbohydrate diet,dietary fiber and legumes were effective to the treatment of diabetes. Prediction of postprandial glucose level and treatment based on gut microbiota contributed to control the glucose of diabetic patients. An in-depth understanding of these mechanisms provides new ideas for the individualized and precise treatment of diabetes.

AIM:The complications following liver transplantation consist of the liver damage and the function failure of other organs,among which lung is the most prone and the earliest affected lesion.By establishing an autologous orthotopic liver transplantation(OLT) model in rats,this study was to investigate the acute lung injury after liver transplantation and the role of alkaline phosphatase(AP) in protection of the injury.METHODS:①The experiments were performed in the Test Animal Center and Digestion Laboratory,Nanfang Hospital,Southern Medical University from May to October in 2007.All the proposals were in accordance with the ethical standard of animals.② Twenty-four SD rats were randomly divided into three groups:sham-operation control group,autologous OLT group and AP group,each containing 8 animals.Autologous OLT models were induced in OLT group and AP group,while control group,the abdomen was only opened and then closed besides the liver lobes were isolated.Lingual vein injection of AP was given in AP group 5 minutes before the blood supply of the liver restored.③The concentration of myeloperoxidase(MPO),malondialdehyde(MDA) and surfactant protein A(SP-A) in lung tissue were respectively examined in each group,lung dry-to-wet weight ratio was also measured.Furthermore,hepatic and lung pathological sections were observed.RESULTS:All the 24 rats were involved in the result analysis.①Compared to control group,the concentration of MPO and MDA in lung tissue were significantly increased in OLT group and AP group(P

Objective To develop developer with fast determining stopped spot of electrolyte electrode in wartime.Methods According to certain proportion configuration,the developer was prepared by using relative reagent and distilled water.Through injector to take 1ml developer and curve syringe needle,then straight insertion the type needle of electrolysis meter and pour into the developer to fill suction tube.Results The reagent can be seen various electrodes change and fast display the electrode breakdown anywhere.Conclusion The color developing reagent is low-cost,efficient and convenient to use.

Objective To explore the effect of baicalin on tumour necrosis factor-?(TNF-?) and soluble inter-cellular adhesion molecule-1(sICAM-1) of immunosuppressed rats infected with Pneumocystis carinii.Methods Forty-nine SD rats were randomly divided into 7 groups:normal control(A), immunosuppressed control(B), SMZ/TMP control(C), baicalin prevention(D), low dose(E), moderate dose(F) and high dose(G).Rats of group D received six injections at three-day intervals with 3.5 mg dexamethasone sodium phosphate for 3 weeks, while groups B, C, E, F and G received same immunosuppressor but for 6 weeks.Rats in group D were given 100 mg/kg baicalin daily for 5 weeks, and groups C, E, F and G were given 250 mg/kg SMZ+50 mg/kg TMP, low dose 100 mg/(kg?d), moderate dose 200 mg/(kg?d), and high dose 400 mg/(kg?d) baicalin daily for 2 weeks, respectively.At the end of 8th week after immunosppression, the contents of TNF-? and sICAM-1 in peripheral blood were detected by radioimmunoassay(RIA) and ELISA, respectively.The pathological change of lung was observed by lung imprint smear with gormori methenamine silver(GMS) staining and lung section with hematoxylin-eosin(HE) staining.Results The content of TNF-? in group D(2.14?0.14) ng/ml, group E(2.57?0.15) ng/ml, group F(1.46?0.14) ng/ml, group G(1.12?0.13) ng/ml andgroup C(1.59?0.14) ng/ml were higher than that of group A(0.70?0.21)ng/ml(P

The application of military medical equipment highly influences the effect of medical support.According to the investigation in the affiliated basic units,this paper puts forward improvement measures for the existing problems in the application of military medical equipment.

Strategic information reservation of medicinal materials is complementary to the strategic reservation of medicinal materials itself.This article discusses the advantages and disadvantages of the reservation of physical medicinal goods and mobilization of medical resources for national defense.The construction mode and operational characteristics of the information database for the strategic reservation of medicinal materials have also been discussed as well as the great significance of its widely application to the information reservation of medicinal materials.

Aim To study the regulation mechanisms of deacetylase inhibitor SAHA in p21WAF1/CIP1 promoter acetylation in breast cancer MCF-7 cells.Methods We used quantitative real-time PCR, Western blot and DNA-ChIP to determine the effects on the regulation of cell cycle with SAHA treatment in MCF-7 cells.By DNA-ChIP, we assessed the acetylation level of p21WAF1/CIP1 promoter.Results SAHA significantly affected the expression of cell cycle-related factors, and induced the mRNA and protein expression of p21WAF1/CIP1.SAHA could adjust the acetylation level of p21WAF1/CIP1 promoter.Conclusion SAHA regulates the cell cycle progression by adjusting the acetylation level of p21WAF1/CIP1 promoter in MCF-7 cells.

BACKGROUND:To delay the onset of Alzheimer’s disease, transplantation of viable and wel-differentiated stem cel s is expected to repair neural tissue, which has been an issue of concern. OBJECTIVE:To explore the effects of different doses of human umbilical cord mesenchymal stem cel s (hUCMSCs) on learning and memory ability of Alzheimer’s disease rats. METHODS:Fifty Sprague-Dawley rats, 7 months of age, were randomized into normal, model, high-, middle-and low-dose hUCMSCs groups (n=10 per group). Rats in model and UCMSCs groups were used to make Alzheimer’s disease animals through intraperitoneal injection of 150 mg/kg D-galactose for 90 days, and rats in the normal group were given intraperitoneal injection of normal saline for 90 days. In the three hUCMSCs group, passage 3 hUCMSCs at doses of 1×105/0.2 mL/20 g, 5×105/0.2 mL/20 g, and 1×106/0.2 mL/20 g were injected via the tail vein, respectively. Forty-five days after cel transplantation, Morris water maze test was used to detect rat’s learning and memory abilities, and hematoxylin-eosin staining was used to observe pathological changes of the rat hippocampal CA1 region. RESULTS AND CONCLUSION:Compared with the normal group, the rats in the model group showed significant reduction in the ability of learning and memory. Compared with the model group, the escape latency was significantly shortened in the middle-dose hUCMSCs group (P<0.05), while the number of passing times through the platform was increased significantly (P<0.05). In the model group, the cel s in the hippocampal CA1 region were arranged irregularly with unclear nucleoli and a part of cel s were concentrated and deeply stained. In the middle-dose hUCMSCs group, the cel s in the hippocampal CA1 region were arranged regularly with clear nucleoli, and only individual cel s were stained deeply. These findings indicate that middle-dose hUCMSCs transplantation can improve the learning and memory abilities of Alzheimer’s rats.