OBJECTIVE: To study the protective effects of nimesulide, a COX- 2 selective inhibitor on experimental gastric ulcer in rats. METHODS: The gastric ulcer model was established in rats by intragastric administration them with indometacin, 5 min later, which were assigned to receive nimesulide at different dosages ( 200, 100, and 50mg? kg- 1) , 6h later, all the rats were sacrificed. The primary outcome measures were the area of ulcer, levels of SOD, MDA, and GSH in sera and gastric mucosa. RESULTS: As compared with model group, in nimesulide ( at different dosage) groups, the area of ulcer was markedly decreased, level of MDA was decreased while activities of SOD and GSH were increased ( P

With the improvement of the therapy and the drugs we known more results in a therapeutic ambitious goals of juvenile idiopathic arthritis.Selection of individualized sequential therapy is beneficial to the recovery of the disease.

Objective To explore the relationship between early neonatal respiratory faster and asthma in children in the future.Methods 82 cases of unexplained breathing faster after babies born were selected as observa-tion group,and 82 normal newborns at the same period were selected as the control group.The newborns were followed up for 4 -5 years,the incidence of neonatal asthma in the future were compared between the two groups.Results The indicators between observation group and control group had statistically significant differences (all P <0.05).In the observation group,there were 11 cases of clinical diagnosis of infantile asthma,the incidence rate was 13.41%(11 /82),and in the control group,there were 2 cases of clinical diagnosis of infantile asthma,the incidence rate was 2.44%(2 /82).The difference between two groups was statistically significant (χ2 =5.35,P <0.05).Conclusion Early neonatal respiratory faster is closely associated with asthma in children in the future.

Objective To analyze the effect of fiber bronchoscope bronchial lavage technique for the children with lobar pneumonia.Methods 110 cases of lobar pneumonia were divided into the bronchial lavage group (55 cases) and the control group (55 cases).The clinical effect of two groups was observed.Results The tempera-ture recovery time, chest rales recovery time, clinical presentation time, X -ray change recovery time of bronchial lavage groups were significantly shorter than those of the control group (all P<0.05).The total effective rate of the lavage group was 85.5%,which was 65.5% of the control group (χ2 =10.632,P <0.01).Conclusion The children with lobar pneumonia taken bronchoscopy and bronchoalveolar lavage reached the diagnosis and achieved the goal of treatment,it could significantly improve the clinical symptoms,promote disease recovery,conducive to rehabili-tation of children.

OBJECTIVE:To evaluate the status quo and the tendency of three-stage analgestics for patients with cancer in our hospitals.METHODS:The use of three-stage analgestics for patients with cancer during the period2002～2005in our hospital were analyzed statistically in respect to consumption sum,DDDs and average daily costs,etc.RESULTS:The con-sumption sum and DDDs of three-stage analgesics increased year on year,of which,morphine and related drugs had gradually dominated the first places.The prices of sustained and controlled release preparations were high.CONCLUSION:The use of three-stage analgestics for patients with cancer in our hospital was rational on the whole,yet the application of three-stage analgestic therapy for pain relief in cancer patients should be further publicized.

OBJECTIVE:To observe the changes of mitochondrial membrane potential(MMP)at different time in the process of simvastatin-induced apoptosis of K562 cells.METHODS:K562 cells cultivated by routine method for 24h were treated in the 20? mol? L-1 simvastatin for 12,24,48,and 72h,respectively.And theh cell morphological changes were observed.Vitality of K562 cells was detected with MTT;flow cytometry were performed to detect cell apoptotic ratio and changes of mitochondrial membrane potential,and the detection results were compared with those in solvent control group.RESULTS:As compared with control group,the morphology changes of K562 cells such as karyopyconosis,nuclear fragmentation and apoptotic body emerged after treatment with 20? mol? L-1 simvastatin for 48h;the apoptotic ratio at 12,24,48,and 72h increased by(2.55? 0.35)%,(6.1? 0.35)%,(14.15? 0.42)%,and(30.70? 0.65)%,respectively;and the apoptotic ratio of K562 cells increased time-dependently.The cell membrane potential was decreased by(0.7? 0.24)%,(39.6? 4.80)%,(24.4? 2.45)%,and(6.0? 1.62)%,respectively,with that at 24h showing the highest decreasing rate.CONCLUSION:The change of MMP is the early events of apoptosis.The potential mechanism of K562 cells apoptosis induced by simvastatin might be related to the collapse of MMP.

OBJECTIVE: To investigate the dissolution in vitro of cefradine capsules from seven pharmaceutical factories for clinical reference in drug choice. METHODS: The dissolution tests were carried out using basket-rotating method. UV spectrophotometry was used to determine the concentration of cefradine. The cumulative dissolution was calculated and dual-index model was used to fit the dissolution parameters (a, b, Td, and T80). The differences among groups were statistically evaluated by variance analysis. RESULTS: The dissolution in vitro of cefradine capsules from seven different pharmaceutical factories were all up to the related standards specified in Chinese Pharmacopoeia(2005 edition). But significant differences were noted in dissolution parameters a,b,Td and T80(P

OBJECTIVE:To validate the feasibility of nonaqueous perchloric acid titration and high performance liquid chromatography(HPLC)for the determination of the content of dipyridamole.METHODS:In the nonaqueous titration,the titration end-point was indicated potentiometrically,and the effects of different solvents on the titration were investigated.As to HPLC method,the separation of sample was performed on C18 column with the mobile phase consisted of acetonitrile-20 mmol?L-1 potassium dihydrogen phosphate(containing 1.0% triethylamine,adjusted pH to 4.0 with phosphoric acid,35∶65).The potassium bromate titration stated in Chinese Pharmacopoeia was taken as the reference.RESULTS:By nonaqueous titration,HPLC and potassium bromate method,the contents of dipyridamole were 101.36%,101.11% and 100.56%,respectively.CONCLUSIONS:Both nonaqueous titration and HPLC methods are suitable for the determination of the content of dipyridamole in its pharmaceutical products.Compared with potassium bromate titration,nonaqueous titration is more simple and convenient.

OBJECTIVE:To provide reference for upgrading emergency management of pharmaceutical affairs and enhancing the safety and effectiveness of clinical medication.METHODS:The guiding effect of evidence-based parmacy(EBP)on emergency management of pharmaceutical affair security especially on the choice of drugs in clinical medication were discussed by elaborating the example of seeking sovereign remedy for tetramine acute poisoning.RESULTS & CONCLUSIONS:Emplying the principle of EBP to guide clinical medication can help to choose more effective and safe drugs and to find the best emergency treatment scheme.

High-quality medical talents must possess high accomplishment of logical thinking,but the current logic thinking standard of medical students is far from the needs of modern medical development.Therefore,setting up of a "logical thinking training" course for medical students is necessary and significant for the innovation,diagnosis,nursing care and management of modern medicine.The theoretical and practical conditions for the new course are both mature with feasibility.