This article analysis the present situation of less than 35 years of Sun Yat-sen university youth scientific and technological personnel,they declare、undertake to support basic research based science fundation related cases,discusses the science foundation for their assistance and training problems,put forward youth scientific talents to suit the science funds development requirements to promote their development proposals

OBJECTIVE:To compare the effects of2different treatment methods on the assaying of triptolide alcohol in tripterygium wilfordii cut crude drug.METHODS:After concentration,tripterygium wilfordii cut crude drug water decoction underwent column chromatographic separation after adsorption with neutral alumina(Method1)and neutral alumina column chromatographic separation after ethanol precipitation and extraction by chloroform(Method2),respectively.Triptolide alcohol was determined by HPLC and the assaying result underwent pairing and t tests.RESULTS:There was significant difference between the2methods with the content determined by Method1significantly higher than that by Method2(P

An experiment on seminal vesiculitis in rats induced by repeated injections of homologous and heterologous seminal vesicle tissue suspension with Freund's complete adjuvant was accomplished to produce an immune disease in the seminal vesicle for the purpose to find the cause of same type of lesion in human beings were divided into 7 In this study 65 healthy adult white rats which weighed from 150 to 250 gm. were divided into 7 groups are follows: Group 1. Six rats, control, injected with normal saline Group 2. Six rats, injected with adjuvant Group 3. Seven rats, injected with seminal vesicle tissue suspension Group 4. Six rats, injected with rat seminal vesicle tissue suspension and adjuvant with prednisolone Group 5. Seven rats, injected with rabbit seminal vesicle tissue suspension Group 6. Seven rats, injected with rabbit seminal vesicle tissue suspension with adjuvant. Rats in these 6 groups were injected weekly for 8 weeks and sacrificed on the sixty fourth day. Group 7. Twenty-six rate, rat seminal vesicle tissue suspension with adjuvant. Rats in this group were subdivided into four groups Group A. Five rats, injected 4 times and sacrificed on 64th day. Group B. Seven rats, injected 6 times and sacrificed on 64th day. Group C. Seven rats, injected 8 times and sacrificed on 64th day. Group D. Seven rats. injected 8 times and sacrificed on 92nd day. Seminal vesicles from all the rats sacrificed were histopathologically studied and results were obtained as follows. Significant histopathologic alterations suck as glandular atrophy, decreased secretoryactivity, interstitial fibrosis, edema. congestion and inflammatory cellinfiltration suggesting of noninfectious chronic seminal vesiculitis were obtained in most rats of Group 7. Severity of inflammatory reaction seemed to be accentuated according to the increase of frequency of the date of sacrifice not concerned. Those allergic reaction were appeared to be inhibited by administration of prednisolone.
Adult ; Animals ; Atrophy ; Edema ; Estrogens, Conjugated (USP) ; Fibrosis ; Humans ; Hypersensitivity ; Immune System Diseases ; Prednisolone ; Rats ; Seminal Vesicles*

Although alpha2-adrenoceptor agonists are widely used as postoperative sedatives in adults, the postoperative effects in pediatric patients with secondary pulmonary arterial hypertension (PAH) due to congenital heart disease are not well known. We experienced a case of successful ventilator weaning with continuous intravenous administration of dexmedetomidine (DEX) after surgical correction in a 46-month-old child with congenital ventricular septal defect (VSD) with severe PAH. She underwent VSD closure on cardiopulmonary bypass (CPB). After successful weaning from the CPB, hemodynamics and oxygenation were stabilized on DEX and nitroglycerin in the intensive care unit. The patient was successfully weaned from the ventilator 46 hours after surgery. The transthoracic echocardiogram two weeks after surgery showed a closed VSD with no residual shunt and trivial tricuspid regurgitation (Vmax = 2.5 m/sec) without PAH.
Administration, Intravenous ; Adult ; Cardiopulmonary Bypass ; Child* ; Child, Preschool ; Dexmedetomidine* ; Heart Defects, Congenital ; Heart Septal Defects, Ventricular* ; Hemodynamics ; Humans ; Hypertension* ; Hypnotics and Sedatives ; Intensive Care Units ; Nitroglycerin ; Oxygen ; Tricuspid Valve Insufficiency ; Ventilator Weaning ; Ventilators, Mechanical ; Weaning

Alteration of H-and M-isozymfs of Lactic Dehydrogenase(LDH) were observed in the various tissues after exposing the rats to 50ppm and 250ppm of sulfur dioxide. These isozymes of the respective tissue were separated by Diethlaminoethyl(DEAE)-cellulose from tile tissue homogenates of brain, lung and muscle, presenting the activities by rate of reduction of nicot inamids-adenine-dinucleotide (NAD+). Pure LDH and the coenzyme NAD (NAD) were directly treated with sulfur dioxide in vitro order to find out the direct to sulfur dioxide on LDH and NAD+ and the results were as follow. 1. In the normal tissues, the H-isozyme activity was dominant in the brain and heart, and tole M-isozyme in the muscle. 2. In the lung tissue of normal rats, there was no different between tole activity of H-and M-type of LDH. 3 When rats inhale sulfur dioxide gas in concentration of 50ppm and 230ppm, it appeared that the H-type tend to be suppressed in aerobic tissues and the M-type in anaerobic tissues. 4. In the lung tissue exposed to sulfur dioxide, both the LDH activities were suppressed. 5. It seems that LDH and the coenzyme (NAD ) are not directly affected by exposing in sulfur dioxide gas.
Animals ; Brain ; Heart ; Isoenzymes ; Lung ; NAD ; Rats ; Sulfur Dioxide* ; Sulfur*

To observe the 36 months follow-up outcome of zirconia all-ceramic posterior fixed dental prostheses(FDPs)designed and manu-factured by computer aided design and computer aided manufacture (CAD /CAM)from clinical aspect,and to evaluate the success rate of zirconia framework and veneering porcelain.78 patients with less than three teeth lost which had natural opposing teeth were included.A to-tal of ninty-two zirconia all-ceramic FDPs (59 three-unit FDPs,20 four-unit FDPs and 13 five-unit FDPs)were made by CAD /CAM.Three years after cementation of the restorations,the reasons for complication and failure of FDPs in different lengths were analysed.Six FDPs were removed (frame fracture:1;porcelain fracture:1;secondary caries:2;vertical root fractures and post-core fracture after root canal therapy of premolars:2).The success rate of these FDPs was 93.5%.The success rate of zirconia all-ceramic crowns and bridges was higher.Their clinical effect was satisfactory.The main complications of these FDPs were porcelain fracture and de-bonding.

Objective To improve the quality of the standard bazheng capsule.Methods TLC was used for identification of emodin and chrysophanol content determination, and determination of samples by high performance liquid chromatography.Results TLC spots were clear,and the blank without interference.Emodin showed a good linear relationship at range of 0.0267 ~0.4272 μg, Y =1425.7X +2.1521 ( r=0.9998 );chrysophanol showed a good linear relationship at range of 0.0417 ~0.6677 μg, Y =5 ×106X -8566.2(r=0.9999).The recovery was 97.35%, 98.39%,and RSD was 1.7%,0.4%.Conclusion The established methods are simple and with good repeatability, and can be used for the quality control of bazheng capluse.

The aim of extended Chinese pattern differetiation method by endoscope is to establish the new system of five methods for clinical Chinese pattern differetiation system. There were two modes of the new system: the first mode is a purely Chinese pattern differentiation of inspectioin of the mucosa by endoscope, and the other mode combines the TCM four methods (inspection, listening and smelling, interrogation, pulse feeling and palpation) with endoscopic inspection method to establish the extended Chinese pattern differentiation mode by endoscope. Key technology was that: (1) how to compare the traditional four methods with the new five methods in clinic; (2) how to set up the criteria and classification of extended Chinese pattern differentiation by endoscope mode; (3) How to improve the new system with the feedback of effect of extended Chinese pattern differentiation by endoscope mode. The new system was regarded as innovative in the fields of clinical practice, TCM theory and application, and it was believed that it would promote TCM in the process of modernaization.

Objective To compare the clinical effects of peritoneal cavity after laparoscopic renal pelvis and ureter lithotomy and traditional open renal pelvis incision nephrolithotomy.Methods 150 cases with renal pelvis and ureteral calculi were randomly divided into control group and observation group with 75 cases in each group,the control group was given traditional open pyelolithotomy for treatment while the observation group was given peritoneal cavity after laparoscopic renal pelvis and ureter lithotomy for treatment,clinical efficacy of the two groups were compared.Results The clinical effective rate of the observation group after treatment was 100％,which that of the control group was 92％,there was significant differences between the two groups (x2 =10.31,P ＜ 0.05).The operation time in the observation group was (90 ± 12) min,amount of bleeding during operation was (25 ± 7) mL,hospital stays was (10 ± 3) d,which were better than those of the control group,there were significant differences between the two groups (t =7.24,8.31,7.61,all P ＜ 0.05).Conclusion Retroperitoneal laparoscopic pyelolithotomy and ureterolithotomy is a minimally invasive operation,which has the advantages of definite curative effect,less trauma,less bleeding,rapid recovery,superior than the traditional open pyelolithotomy,so it is worthy of clinical application.

Objective]To observe the clinical effect of chief physician Zhao Runchen’s therapy of nerve root type cervical spondylosis.[Methods]According to the random and parallel control test principles, 48 cases of patients with nerve root type cervical spondylosis were divided into treatment group and control group. The treatment group was treated with conventional massage combined with pulling therapy, and the control group was treated with conventional massage. After treatment every other day for 20 times, the clinical effect of the two groups and the visual analogue scale before and after treatment were observed.[Results]15 cases in the treatment group were cured, 6 cases improved, so the basic cure rate was 63%, and total effective rate was 88%. 11 cases in the control group were cured, 9 cases improved, so the basic cure rate was 46%, and total effective rate was 83%. Cure rate between the two groups had significant difference(P<0.01), and VAS score of both groups were significantly improved after treatment: the control group decreased significantly(P<0.05), and the treatment group showed extremely significant decrease(P<0.01). [Conclusion]Chief physician Zhao Runchen’s pulling therapy of nerve root type cervical spondylosis has a satisfactory basic cure rate.