1.Cord blood as a valuable source of hemopoietic stem cell for bone marrow transplantation
Journal of Vietnamese Medicine 1999;233(2):19-21
We studied on hematological parameters, lymphocytes distribution and stem cell (CD34 positive) count from cord blood of 43 healthy newborn at obstetrics department, Bach Mai hospital. The result showed that, stem cell count of cord blood is high (16.2% of nucleated cell, equal to 2.4+/- 0.3 G/l). this will be useful to allegoric stem cell transplantation. However, relatively high count of white cell (especially T lymphocyte) can be considerable to procedure and transplant result, free of graft versus host disease.
Fetal Blood
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Bone Marrow Transplantation
2.The results of studying procedure to reduce erythrocytes from cord blood using to long-term storage of CD34 cells
Dung Thi My Pham ; Phan Trung Do ; Tung Quang Nguyen ; Vinh Quang Pham ; Tri Anh Nguyen
Journal of Medical Research 2007;51(4):1-4
Background: The reduction of erythrocytes from cord blood is very need for long - term storage of C034 cells for transplantation. Reduced erythrocyte will reduces preservative blood volume, preservatives and freely HST when defrosting, so stem cells are better protected. Objectives: To study selection of the best centrifugal procedure to reduce maximal erythrocytes and lose minimal C034 cells from cord blood. Subjects and methods: 20 blood samples selected from 60 cord blood units was used for this study. The study was carried out through two steps. In the first step, the centrifugal speed was fixed and the centrifugal time was changed.In the second step, the centrifugal time was fixed, the centrifugal speed was changed. From collected results the best appropriate procedure to reduce erythrocytes from cord blood have been selected. Results: The procedure of gradient centrifuge with speed of 500g in 6 minutes isolated> 50% of erythrocytes, kept > 84% of CD34 cells and then centrifuge of 1000 g in 10 minutes reduced about 40% of volume of nuclear cell - suspension. Conclusion: The procedure can use for preparation of stem cell suspension from cord blood to storage in nitrogen liquid. \r\n', u'\r\n', u'
Erythrocytes/ pathology
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Fetal Blood/ chemistry
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drug effects
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immunology
3.Phenolic Constituents from Balanophora laxiflora with their Anti-inflammatory and Cytotoxic Effects
Nguyen Thi HONG ANH ; Nguyen Thuy DUONG ; Pham Duc VINH ; Do Thi HA
Natural Product Sciences 2021;27(1):49-53
Balanophora laxiflora Hemsl. (Balanophoraceae) is a traditional medicinal plant with a diverse array of biological activities. In our exploration of new bioactive constituents from B. laxiflora, we isolated five compounds, including a new lignan, balanophorone (5), and four known phenolic compounds (1–4). The chemical structures of these compounds were determined by extensive spectroscopic analyses, including 1D and 2D NMR, HR-ESI-MS, and CD. In addition, we evaluated the effects of each of the isolates (1–5) on the messenger RNA expression levels of tumor necrosis factor (TNF)-α and cyclooxygenase (COX)-2 in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and cytotoxicity against MCF-7 and MDA-MB-231 breast cancer cells. Compound 2 showed significant inhibition of LPS-induced COX-2 and TNF-α expression in RAW 264.7 macrophages, while compound 4 showed moderate cytotoxicity against MCF-7 and MDA-MB-231 breast cancer cells, with IC 50 values of 18.3 and 30.7 μM, respectively. No significant effects on the viability of normal mammary epithelial cells were observed.
4.Phenolic Constituents from Balanophora laxiflora with their Anti-inflammatory and Cytotoxic Effects
Nguyen Thi HONG ANH ; Nguyen Thuy DUONG ; Pham Duc VINH ; Do Thi HA
Natural Product Sciences 2021;27(1):49-53
Balanophora laxiflora Hemsl. (Balanophoraceae) is a traditional medicinal plant with a diverse array of biological activities. In our exploration of new bioactive constituents from B. laxiflora, we isolated five compounds, including a new lignan, balanophorone (5), and four known phenolic compounds (1–4). The chemical structures of these compounds were determined by extensive spectroscopic analyses, including 1D and 2D NMR, HR-ESI-MS, and CD. In addition, we evaluated the effects of each of the isolates (1–5) on the messenger RNA expression levels of tumor necrosis factor (TNF)-α and cyclooxygenase (COX)-2 in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and cytotoxicity against MCF-7 and MDA-MB-231 breast cancer cells. Compound 2 showed significant inhibition of LPS-induced COX-2 and TNF-α expression in RAW 264.7 macrophages, while compound 4 showed moderate cytotoxicity against MCF-7 and MDA-MB-231 breast cancer cells, with IC 50 values of 18.3 and 30.7 μM, respectively. No significant effects on the viability of normal mammary epithelial cells were observed.
5.The Effects of Irisin on the Interaction between Hepatic Stellate Cell and Macrophage in Liver Fibrosis
Dinh Vinh DO ; So Young PARK ; Giang Thi NGUYEN ; Dae Hee CHOI ; Eun-Hee CHO
Endocrinology and Metabolism 2022;37(4):620-629
Background:
Hepatic stellate cells (HSCs) are the central players interacting with multiple cell types in liver fibrosis. The crosstalk between HSCs and macrophages has recently become clearer. Irisin, an exercise-responsive myokine, was known to have a potentially protective role in liver and renal fibrosis, especially in connection with stellate cells. This study investigated the effects of irisin on the interaction between HSCs and macrophages.
Methods:
Tamm-Horsfall protein-1 (THP-1) human monocytes were differentiated into macrophages, polarized into the inflammatory M1 phenotype with lipopolysaccharide. Lieming Xu-2 (LX-2) cells, human HSCs, were treated with conditioned media (CM) from M1 macrophages, with or without recombinant irisin. HSCs responses to CM from M1 macrophages were evaluated regarding activation, proliferation, wound healing, trans-well migration, contractility, and related signaling pathway.
Results:
CM from M1 macrophages significantly promoted HSC proliferation, wound healing, transwell migration, and contractility, but not activation of HSCs. Irisin co-treatment attenuated these responses of HSCs to CM. However, CM and irisin treatment did not induce any changes in HSC activation. Further, irisin co-treatment alleviated CM-induced increase of phopho-protein kinase B (pAKT), matrix metalloproteinase-9 (MMP-9), and tissue inhibitor of metalloproteinases-1 (TIMP-1).
Conclusion
These findings suggested that irisin may play a protective role in the pathogenesis of liver fibrosis, especially when working in the crosstalk between HSCs and macrophages.
6.Gemigliptin Alleviates Succinate-Induced Hepatic Stellate Cell Activation by Ameliorating Mitochondrial Dysfunction
Giang NGUYEN ; So Young PARK ; Dinh Vinh DO ; Dae-Hee CHOI ; Eun-Hee CHO
Endocrinology and Metabolism 2022;37(6):918-928
Background:
Dipeptidyl peptidase-4 inhibitors (DPP-4Is) are used clinically as oral antidiabetic agents. Although DPP-4Is are known to ameliorate liver fibrosis, the protective mechanism of DPP-4Is in liver fibrosis remains obscure. In this study, gemigliptin was used to investigate the potential of DPP-4Is to alleviate the progression of liver fibrosis.
Methods:
To clarify the effects and mechanisms of gemigliptin, we conducted various experiments in LX-2 cells (immortalized human hepatic stellate cells [HSCs], the principal effectors of hepatic fibrogenesis), which were activated by succinate and exhibited elevated expression of α-smooth muscle actin, collagen type 1, and pro-inflammatory cytokines and increased cell proliferation. In vivo, we examined the effects and mechanisms of gemigliptin on a high-fat, high-cholesterol–induced mouse model of nonalcoholic steatohepatitis (NASH).
Results:
Gemigliptin decreased the expression of fibrogenesis markers and reduced the abnormal proliferation of HSCs. In addition, gemigliptin reduced the succinate-induced production of mitochondrial reactive oxygen species (ROS), intracellular ROS, and mitochondrial fission in HSCs. Furthermore, in the mouse model of NASH-induced liver fibrosis, gemigliptin alleviated both liver fibrosis and mitochondrial dysfunction.
Conclusion
Gemigliptin protected against HSC activation and liver fibrosis by alleviating mitochondrial dysfunction and ROS production, indicating its potential as a strategy for preventing the development of liver disease.
7.Dirofilariasis in Vietnam: A case report and brief review
Que, A.T. ; Nguyen, D.N. ; Do, N.A. ; Le, T.A.
Tropical Biomedicine 2019;36(2):475-481
This report describes a rare case of ophthalmic dirofilariasis in a 68-year-old
woman with red and foreign body sensation in the pterygium on her right eye. Slit lamp
examination demonstrated a long-slender worm moving in her pterygium. The worm was
removed surgically and then identified as Diroflaria repens by sequence analysis of the
small subunit ribosomal RNA (SSU) gene. The situation of dirofilariasis in Vietnam has
been reviewed. Since the first described case in 2010 there have been thirteen cases
reported that suggested the emerging trend of the disease. Most of the documented cases
of human dirofilariasis recorded in Vietnam presented with ocular infections and the
responsible agent was D. repens. With the increase of reported cases of human, much more
attention should be paid on control as well as diagnosis and treatment of dirofilariasis in
Vietnam.
8.Human Taeniasis and Cysticercosis and Related Factors in Phu Tho Province, Northern Vietnam
Vu Thi Lam BINH ; Do Trung DUNG ; Hoang Quang VINH ; Van Hul ANKE ; Praet NICOLAS ; Dorny PIERRE ; Dermauw VERONIQUE
The Korean Journal of Parasitology 2021;59(4):369-376
Several factors presumed to facilitate the transmission of Taenia spp. were reported in Vietnam. We conducted a cross-sectional study taking questionnaires from 1,185 participants, and collecting 1,151 sera and 1,036 stool samples in northern Vietnam. Sera were examined for circulating antigens of Taenia solium cysticerci using ELISA, stools for Taenia eggs by Kato-Katz smear, and copro-antigens by ELISA. Ag-ELISA revealed 4.6% antigen positivity, indicating infection with viable cysticerci. Taenia eggs were detected in 1.5% of participants. Copro-antigens were found in 2.8% of participants. Eating raw meat and/or vegetables was significantly associated with the presence of copro-antigen (OR=8.6, 95% CI: 1.16-63.9, P=0.01). Considering the high taeniasis prevalence and the associated threat, public health attention should be given to treat the tapeworm carriers in the projected areas.
9.Human Taeniasis and Cysticercosis and Related Factors in Phu Tho Province, Northern Vietnam
Vu Thi Lam BINH ; Do Trung DUNG ; Hoang Quang VINH ; Van Hul ANKE ; Praet NICOLAS ; Dorny PIERRE ; Dermauw VERONIQUE
The Korean Journal of Parasitology 2021;59(4):369-376
Several factors presumed to facilitate the transmission of Taenia spp. were reported in Vietnam. We conducted a cross-sectional study taking questionnaires from 1,185 participants, and collecting 1,151 sera and 1,036 stool samples in northern Vietnam. Sera were examined for circulating antigens of Taenia solium cysticerci using ELISA, stools for Taenia eggs by Kato-Katz smear, and copro-antigens by ELISA. Ag-ELISA revealed 4.6% antigen positivity, indicating infection with viable cysticerci. Taenia eggs were detected in 1.5% of participants. Copro-antigens were found in 2.8% of participants. Eating raw meat and/or vegetables was significantly associated with the presence of copro-antigen (OR=8.6, 95% CI: 1.16-63.9, P=0.01). Considering the high taeniasis prevalence and the associated threat, public health attention should be given to treat the tapeworm carriers in the projected areas.
10.Impact of long COVID-19 on posttraumatic stress disorderas modified by health literacy: an observational study inVietnam
Han Thi VO ; Tien Duc DAO ; Tuyen Van DUONG ; Tan Thanh NGUYEN ; Binh Nhu DO ; Tinh Xuan DO ; Khue Minh PHAM ; Vinh Hai VU ; Linh Van PHAM ; Lien Thi Hong NGUYEN ; Lan Thi Huong LE ; Hoang Cong NGUYEN ; Nga Hoang DANG ; Trung Huu NGUYEN ; Anh The NGUYEN ; Hoan Van NGUYEN ; Phuoc Ba NGUYEN ; Hoai Thi Thanh NGUYEN ; Thu Thi Minh PHAM ; Thuy Thi LE ; Thao Thi Phuong NGUYEN ; Cuong Quoc TRAN ; Kien Trung NGUYEN
Osong Public Health and Research Perspectives 2024;15(1):33-44
Objectives:
The prevalence of posttraumatic stress disorder (PTSD) has increased, particularly among individuals who have recovered from coronavirus disease 2019 (COVID-19) infection. Health literacy is considered a “social vaccine” that helps people respond effectively to the pandemic. We aimed to investigate the association between long COVID-19 and PTSD, and to examine the modifying role of health literacy in this association.
Methods:
A cross-sectional study was conducted at 18 hospitals and health centers in Vietnamfrom December 2021 to October 2022. We recruited 4,463 individuals who had recovered from COVID-19 infection for at least 4 weeks. Participants provided information about their sociodemographics, clinical parameters, health-related behaviors, health literacy (usingthe 12-item short-form health literacy scale), long COVID-19 symptoms and PTSD (Impact Event Scale-Revised score of 33 or higher). Logistic regression models were used to examine associations and interactions.
Results:
Out of the study sample, 55.9% had long COVID-19 symptoms, and 49.6% had PTSD.Individuals with long COVID-19 symptoms had a higher likelihood of PTSD (odds ratio [OR], 1.86; 95% confidence interval [CI], 1.63–2.12; p < 0.001). Higher health literacy was associated with a lower likelihood of PTSD (OR, 0.98; 95% CI, 0.97–0.99; p = 0.001). Compared to those without long COVID-19 symptoms and the lowest health literacy score, those with long COVID-19 symptoms and a 1-point health literacy increment had a 3% lower likelihood of PTSD (OR, 0.97; 95% CI, 0.96–0.99; p = 0.001).
Conclusion
Health literacy was found to be a protective factor against PTSD and modified the negative impact of long COVID-19 symptoms on PTSD.