Acupuncture Points ; Acupuncture Therapy ; Adolescent ; Adult ; Female ; Humans ; Hysteria ; therapy ; Male ; Middle Aged ; Paralysis ; therapy ; Young Adult

The silver doped poly ( L-lysine ) modified glassy carbon electrode was fabricated by cyclic voltammetry, the surface of the electrode was characterized by scanning electron microscopy and electrochemical impedance spectroscopy. The electrochemical behaviors and the simultaneous detection of xanthine and uric acid were studied by cyclic voltammetry and differential pulse voltammetry. The results indicated that this modified electrode exhibited excellent electrocatalytic activity towards the oxidation of xanthine and uric acid. The stable oxidation peaks of xanthine and uric acid appeared with the peak potential of 0. 980V and 0. 600V respectively at the modified electrode in pH 3. 0 phosphate buffer solution. The oxidation peaks of xanthine and uric acid were separated at 380 mV. Under the optimum conditions, the linear ranges for the determination of xanthine and uric acid were 1 . 00 × 10-6-2 . 50 × 10-4 mol/L respectively by differential pulse voltammetry. The detection limits were 5. 0×10-7mol/L. The method has been applied to the simultaneous detection of xanthine and uric acid in healthy human urine with satisfactory results.

Objective To evaluate the efficacy of deanxit in treatment of post-stroke depression (PSD).Methods60 patients with PSD were randomly divided into treatment group ( plus with deanxit) and control group (plus with estazolam necessarily). At the baseline and at 2 weeks,4 weeks, 8 weeks after the treatment, the efficacy was assessed with HAMD, SSS, and ADL rating scale respectively. Results After 2,4,8 weeks treatment, HAMD and SSS scores of the treatment group significantly decreased compared with the control group ( all P ＜ 0. 01 ), and ADL scores of the treatment group increased significantly compared with the control group ( P ＜ 0. 01 ). Conclusion Deanxit could effectively relieve depressing symptoms and neurological impairment of PSD, and clinical side effects of deanxit were less. Deanxit was suitable for acute phase and long-term maintenance therapy in PSD.

Objective To observe the value of detection of pulmonary arterial hypertension(PAH) with curvature ratio measured at cardiac MR.Methods We performed a retrospective study of 48 patients with cardiac MR,systemic systolic blood pressure and right heart catheterization(RHC) data.The parameter curvature ratio (Rc) was measured at cardiac MR and transseptal/transmural pressure ratio (RP) was calculated.By using the regression analysis between Rc and RP,the SPAP was calculated.Bland-Altman analysis was used to compare the SPAP calculated with SPAP obtained by RHC.The accuracy of the SPAP calculated in detecting PAH was analyzed by using receiver operating characteristic (ROC) curves (SPAP higher than 40 mmHg as cutoff).The normality of parameter is tested by K-S test.Bivariate correlation Pearson test was used to test the consistency of parameters obtained by different measurers.Linear regression analysis was performed to find the relationship between Rc and RP.Results Parameter RP =0.28 ± 0.37 (-0.50-0.83) and RC =0.31 ± 0.57 (-0.78-0.97).Linear relationship was found between Rc and RP (r =0.94,P ＜ 0.01) and regression equation:Rc =1.44 × RP-0.09.Bland-Altman analysis showed satisfactory limits of agreement with the SPAP calculated and obtained by RHC.ROC analysis of the diagnostic value in PAH revealed 94.4％ (34/36) of sensitivity and 100.0％ of specificity (12/12,area under ROC curve =0.942,P ＜ 0.01).Conclusions Our study suggests that with curvature ratio measured at cardiac MR,the SPAP estimated have considerable sensitivity and specificity for detecting PAH.It may be a useful non-invasive assessment in patients that suspected of PAH.

Objective:To examine the expression of integrin ?V?3 in periodontium during experimental tooth movement, and then to propose the possible role of ?V?3 in bone remodeling during tooth movement. Methods:Thirty-five rabbits were divided into 7 groups (5 rabbits per group):untreated(control), 1,3, 5, 7, 14 and 21 d groups. A elastic coil spring was ligated between the incisors and the first molar and exert a force of approximately 0.78 N to pull the first molar mesially. The animals were sacrificed at each time intervals(1,3,5,7,14 and 21 d) respectively and specimens were made to observe the expression of integrin ?V?3 in periodontium through in situ hybridization. Results:Different extent expression of integrin ?V?3 were seen in osteoclasts, osteoblast precursors, osteoblasts,odontoclasts and odontoblast except bone cells. Conclusions: Integrin ?V?3 may play an important role in bone modeling, cementum resorption and its reparation during experimental tooth movement.

[A Review] Many clinical and basic researches have revealed that brain damage can be deteriorated by diabetes significantly. However, its pathogenesis remains unclear. Recently, apoptosis have become the focus of research on brain damage. This article introduces the related investigations.

Objective To explore the test-retest reliability of elevated plus maze (EPM) which is taken as an animal model of state anxiety in Kunming mice.Methods The behavior of adult,male and female,Kunming mice in EPM was recorded twice with one-week inter-trial interval for lasting five minutes.The following parameters were evaluated:percentage of time on EPM open arms (Otime％),percentage of time on EPM closed arms (Ctime％),time on EPM open arms (Otime),time on EPM closed arms (Ctime),percentage of entries into EPM open arms (Oentries％),entries into EPM open arms (Oentries),entries into EPM closed arms (Centries),total entries into EPM open and closed arms (Entries).Subsequently,the intraclass correlation coefficient (ICC) and the kappa agreement index (κ) were calculated for these parameters.Results ICC for Otime％ (♂ ICC=0.506,P＜0.05;♀ ICC=0.456,P＜0.05;♂ + ♀ ICC=0.492,P ＜0.01),Oentries (♂ ICC=0.673,P＜0.01;♀ ICC=0.648,P＜0.01;♂ + ♀ ICC=0.665,P＜0.01),Centries (♂ ICC=0.704,P＜0.01;♀ ICC=0.661,P＜0.01;♂ + ♀ ICC=0.686,P＜0.01),Entries (5 ICC=0.701,P＜0.01;♀ ICC=0.661,P＜0.01;5 + ♀ ICC=0.685,P＜0.01) showed a fair test-retest reliability,respectively.Only κ for Entries (♂ κ=0.253,P＜0.05;♀ κ=0.339,P＜0.05;♂ + ♀ κ=0.298,P＜0.01)displayed a significant but poor reliability between the test-retest trials.Conclusion The Otime％ Entries parameters as an acceptable index for Kunming mice in EPM can be used for exploratory and locomotion activity.Sex and repeated measurement have different effects on the Otime％ and Entries parameters,especially the more on the former.Therefore,Otime％ and Entries can be considered when EPM as an animal model of state anxiety in Kunming mice;meanwhile,more attention should be paid to both the sex (male animals recommended to EPM,especially) and repeated measurement effects.

Heart Septal Defects, Ventricular ; genetics ; pathology ; Humans

Objective To investigate the causes of the complications occurred after repeated embolization therapy for huge cerebral arteriovenous malformations and to discuss their nursing interventions. Methods A total of 54 embolization procedures were performed in 17 patients with huge cerebral arteriovenous malformations. The clinical data were retrospectively analyzed. The causes of complications were carefully examined and the preventive measures were discussed. The prompt and necessary nursing interventions were formulated in order to prevent the complications or serious consequences. Results Among the total 17 patients, one patient gave up the treatment because of the cerebral hemorrhage which occurred two months after receiving 3 times of embolization therapy. One patient experienced cerebral vascular spasm during the procedure, which was relieved after antispasmodic medication and no neurological deficit was left behind. Two patients developed transient dizziness and headache, which were alleviated spontaneously. One patient presented with nervousness, fear and irritability, which made him hard to cooperate with the operation and the basis intravenous anesthesia was employed. No complications occurred in the remaining cases. Conclusion The predictive nursing interventions for the prevention of complications are very important for obtaining a successful repeated embolization therapy for huge cerebral arteriovenous malformations, which will ensure that the patients can get the best treatment and the complications can be avoided.

Objective To establish the optimal extraction technology for Yishen Jiangu Granules.Methods The orthogonal test was employed for selecting the optimum of extraction technology. The investigation factors were the consumption of water, the times and time of extraction. The extraction technology of Yishen Jiangu Granules was screened by the transfer rate of icariin in herb epimedii and yield of extraction.Results The best extraction condition was as follows:10 times of water, extracted for 3 times , each time was 3 h,2 h and 2 h. Conclusion This extracting technology for Yishen Jiangu Granule was objective, feasible, stable,reasonable and with good reproducibility.