BACKGROUND:Under normal physiological conditions, there is a homeostasis between the osteogenic and adipogenic differentiation of human bone marrow mesenchymal stem cel s. Osteogenic differentiation is an important process in the formation of skeleton in which differentiated and mature osteoblasts are indispensable. OBJECTIVE:To explore the role of miR-106a and its target gene, bone morphogenetic protein receptor 2 (BMPR2) in the differentiation of human bone marrow mesenchymal stem cel s into osteoblasts. METHODS:Human bone marrow mesenchymal stem cel s were induced to differentiate into osteoblasts by osteoblast-specific induction medium, and this process was detected by western blot and alkaline phosphatase staining. Overexpression of miR-106a was elicited by transfecting miR-106a mimics and the BMPR2 knockdown achieved by RNA interference. The expression levels of miR-106a and BMPR2 were detected by real-time PCR and western blot, respectively. The interaction of miR-106a and BMPR2 was verified by TargetScan software and dual luciferase report experiment assay. RESULTS AND CONCLUSION:The expression of miR-106a was decreased whereas the expression of BMPR2 increased with the progress of osteogenesis differentiation. When miR-106a was overexpressed, alkaline phosphatase activity was declined and the expressions of runt-related transcription factor 2 and osteocalcin, markers of osteogenesis differentiation, both decreased. The expression of BMPR2 was decreased as wel . BMPR2 was predicted to be the target gene of miR-106a by TargetScan software and this prediction proved by dual luciferase report experiments assay. Additional y, osteogenesis differentiation was inhibited by knocking down the expression of BMPR2. These results indicate that miR-106a regulates the differentiation of bone marrow mesenchymal stem cel s into osteoblasts by targeting BMPR2.

Objective To study the clinical efficacy of acupuncture combined with Rood therapy for post-stroke upper limb functional disorder. Methods Ninety patients which met the inclusion criteria were randomly divided into integrated group (acupuncture +Rood therapy), acupuncture group, and Rood therapy group, 30 in each group. The therapeutic effect of the three groups was evaluated after treatment for 6 courses, one course covering 7 days. Results (1) After treatment for 6 courses, the total effective rate of the integrated group, acupuncture group, and Rood therapy group was 93.33%, 76.67%, 63.33%, respectively. The results of Mann-Whitney test showed that the integrated group had better effect than acupuncture group and Rood therapy group, the difference being significant(P<0.05).(2) After treatment, muscle tension of the integrated group and acupuncture group was much improved as compared with that before treatment(P<0.01), but Rood therapy group showed no obvious changes in muscle tension before and after treatment(P>0.05). (3) After treatment, FMA scores and MBI scores were markedly increased in the three groups(P<0.001 compared with those before treatment). The integrated group had better effect on improving FMA scores and MBI scores than acupuncture group and Rood therapy group , and the resul ts of ANOVO test showed that t he differences were signficiant(P<0.05). Conclusion Acupuncture combined with Rood therapy has certain clinical efficacy for post-stroke upper limb functional disorder, and the effect is better than acupuncture or Rood therapy alone.

Objective:To explore the influence of cardiac pacing at different sites on left atrial structure and function . Methods:A total of 62 patients ,who needed cardiac pacing therapy because of bradycardia in our hospital from Jan 2012 to Jan 2014 ,were selected .According to the implanted sites of pacing electrode ,patients were equally divided into right ventricular cardiac apex group (RVA group) and right ventricular outflow tract group (RVOT group) . Left atrial volume before atrial contraction (LAVp) ,minimum left atrial volume (LAVmin) ,maximum left atrial volume (LAVmax) ,left atrial active emptying fraction (LAAEF) and left atrial passive emptying fraction (LA-PEF) etc .were compared between two groups before and after cardiac pacing therapy .Results:There were no sig-nificant difference in all indexes between two groups before treatment ,P>0.05 all .Compared with RVA group af-ter six-month pacing treatment ,there were significant reductions in LAVp [ (29.5 ± 3.2) ml vs .(27.1 ± 3.5) ml] , LAVmin [ (18.6 ± 2.0) ml vs . (17.2 ± 2.7) ml] ,LAVmax [ (48.9 ± 4.8) ml vs .(46.1 ± 5.1) ml] and LAAEF [(47.2 ± 4.5)% vs .(43.5 ± 4.2)% ] ,and significant rise in LAPEF [(40.9 ± 5.3)% vs .(43.6 ± 3.9)% ] in RVOT group ,P<0.05 or <0.01. Conclusion:Effect of right ventricular outflow tract pacing is more than that of right ventricular cardiac apex pacing and may be used as a replacement of right ventricular cardiac apex .

The iodine deficiency disorders surveillances were performed in different historical periods according to different surveillance plans in China as various monitoring purposes.Compared with other editions of surveillance plan,the biggest changes were to observe the urinary iodine level,the salt iodine level and the goiter rate in children and pregnant women in the county level in this National Iodine Deficiency Disorders Surveillance Plan (the 2016 edition).In this paper,the implementation details on this surveillance plan were further interrelated,which would play an instrumental role for the staff in iodine deficiency disorders prevention and control.

ICU is the important place of ventilator associated pneumonia, the occurrence of fatality rate in patients with ventilator associated pneumonia is higher. In this paper, the cause of the ventilator associated pneumonia in ICU and nursing research progress are summarized, that enhance their awareness of the importance of nursing care and the use of normative evidence-based nursing in clinical practice thinking and nursing process, explore the nursing methods of more appropriate, to reduce the incidence of ventilator associated pneumonia in ICU is of great help.

Objective To study the health situation of the rural women in Luokeng town of Jiangmen city and provide the reference for the prevention and control of rural women to disease .Methods The 2 000 cases of rural women in Luokeng town of Jiangmen city during the period from Jan 2012 to Dec 2013 were received the routine gyne-cological examination and questionnaire survey .The data were retrospectively analyzed .Results The 1066 women were gynecological diseases (53.3%) in the 2 000 cases including 504 cases of vaginitis, 344 cases of cervicitis, 72 cases of uterine fibroids , 66 cases of pelvic inflammatory disease , 52 cases of cervical polyp and 28 cases of others . The high prevalence was in the 20~35 age group .The rural women′s health knowledge was low and the treatment compliance was relatively poor .Conclusion The prevalence rate of ural women in Luokeng town of Jiangmen city is relatively high .The gynecological health education should strengthen and improve the levels of prevention of gyneco -logical diseases .

Objective To probe the application and values of multilayer spiral CT three -dimensional recon-struction of post-processing in the diagnosis of bone tumor images .Methods 54 bone tumor patients in our hospital from August 2013 to August 2014 were taken as research objects and they were randomly into two groups with same members.Patients in observation group were conducted with multilayer spiral CT three -dimensional reconstruction diagnosis while patients in control group were conducted with MRI diagnosis .The diagnosis Results were analyzed . Result Diagnosis effective rate in observation group was 85.2%, with 59.3%in control group.Differences were evi-dent ( p<0.05) and had statistical significance .Conclusion Applying multilayer spiral CT three -dimensional re-construction of post-processing in diagnosis of bone tumor can improve diagnosis accuracy rate , and correctly judge disease extents , which can provide effective gist for clinical treatment .

The patients with primary enuresis complicating with hemirachischisis were treated by routine manipulations as well as the acupoints and manual methods to unblock and regulate Governor Vessel. Excellent effects have been obtained.

microRNA (miRNA) refers to small (19-25 nt) single-stranded noncoding RNA molecules that work as post-transcriptional regulator for gene expression by binding to 3' untranslated regions of target mRNA,thus inducing its digestion,degradation and translational repression.Many studies have confirmed that dysregulation of miRNA expression can cause disruption of the hematopoietic system and contribute to leukemogenesis by regulating the expression of some oncogenes and anti-oncogenes.The unique miRNA expression profiles associated with cytogenetic aberrations and mutational status of protein-coding genes may reveal insight into the biology of these leukemia types.This review discussed the latest studies on miRNA expression in different cytogenetic and molecular subtypes of acute myelocytic leukemia and acute lymphoblastic leukemia.

Saxagliptin belongs to the class of dipeptidyl peptidase-4(DPP-4) inhibitors which are novel oral glucose-lowering agents with uniquely mechanism in patients with type 2 diabetes mellitus. Clinical efficacy without increasing hypoglycemia of saxagliptin as single agent as well as in combination with other medications used for the treatment of type 2 diabetes mellitus has been well established in several randomized trials. In addition, a cardiovascular outcome study of SAVOR shows that saxagliptin does not increase the risk of a composite of non-fatal myocardial infarction, non-fatal stroke or cardiovascular death meeting the primary safety objective. The efficacy and safety studies were hereby reviewed.