Currently targeted therapy of breast cancer therapy has become the hot research field and a kind of brand-new biological treatment mode after three traditional pattern of the surgery, radiotherapy and chemotherapy for breast cancer. Molecular targeted therapy is aimed at the target that may cause cancer cells, such as protocarcinogenic genes and tumor-suppressor genes, cell signaling pathways, cytokines and receptors, antiangiogenesis etc. It can reverse malignant biology behavior to inhibit tumor cell growth from the molecular level and has the advantages of high specific effects and low side effects. This paper focuses on the drug of the molecular targeted therapy for breast cancer and the latest progress of targeted therapy.

OBJECTIVE: To establish a bacterial endotoxin test method for fluorescein sodium injection.METHODS: Based on the bacterial endotoxin test method stated in Chinese Pharmacopoeia 2005 edition(second part),an interference test was conduced on different batches of fluorescein sodium samples using tachypleus amebocyte lysate from different companies.RESULTS: At a concentration of no more than 0.75 mg?mL-1,fluorescein sodium showed no interfere with the bacterial endotoxin test.CONCLUSION: It is feasible for bacterial endotoxin test of fluorescein sodium injection be conducted in a limit value of 0.15 EU?mL-1.

Ischemic stroke is one of the major diseases of causing adult disability and death.Age,sex,smoking,hypertension,and diabetes,etc.are the risk factors for ischemic stroke,but they can only partially explain the reasons of stroke onset.Twins,families,and single-gene genetic stroke studies have shown that ischemic stroke has obvious genetic predisposition.In recent years,genomics has made remarkable progress in ischemic stroke study and has shown that many new single nucleotide polymorphisms or genes are associated with ischemic stroke and its risk factors.However,the contribution of these genetic genes for ischemic stroke is still too small and the repetitive studies are needed for further confirmation.

Objective To establish the quality standard for Kangyuan Granula. Methods Radix Astragali, Radix Paeoniae Alba, Fructus Lycii and Radix et Rhizoma Glycyrrhizae in this prescription were identified by TLC. The content of the Paeoniflorin was determined by HPLC. The column of AichromBond-AQ-C18 (4.6 mm?150 mm, 5 ?m) was used, the mobile phase was Water-Acetonitrile (85∶15), at the flow of 1.0 mL/min, the column temperature of 30 ℃, and peaks were detected at 230 nm. Results The characteristic of identification by TLC was distinct and highly specific. The linear range of Paeoniflorin was 0.0545～0.545 ?g, r =0.999 5, the average recovery was 97.90%, RSD=1.06% (n =6). Conclusion The method was easy to operate with accurate result and good reproducibility. It can be used effectively for the quality control of this preparation.

Acupuncture Points ; Acupuncture Therapy ; Humans ; Male ; Middle Aged ; Multiple System Atrophy ; therapy ; Treatment Outcome

Objective To explore the change regulations of inorganic elements in smooth muscle cells of sever scalded rats′gas‐trointestinal tracts and the effects of rhubarb on promoting bowel movement .Methods A total of 68 healthy male rats were select‐ed ,8 rats were enrolled in the healthy control group ,the other 60 rats were selected to establishing 30% scalded models .The trea‐ting scalded group(30 rats) were treated by tube‐feeding of extracting solution of rhubarb ,while the scalded control group(30 rats) were given commensurable distilled water .The concentrations of Cu2+ 、Zn2+ 、Ca2+ and Mg2+ in smooth muscle cells of gastrointes‐tinal tracts were determined 6 ,12 ,24 ,48 and 72 h after establishing scalded rats .Results The concentrations of Cu2+ ,Zn2+ and Ca2+ were decreased ,while the concentration of Mg2+ was initially increased and then decreased ,statistically significant differences were found when compared with the healthy control group (P< 0 .05) .12 h after scalding ,concentrations of these inorganic ele‐ments of rats in the treating scalded group were almost back to normal levels ,and there were statistically significant differences when compared with the scalded control group at any time points (P<0 .05) .Conclusion It is shown that concentrations of inor‐ganic elements in smooth muscle cells of rats′gastrointestinal tracts change obviously at the early stage of sever scald ,and early treatment with rhubarb could regulate levels of inorganic elements .

Objective:To prepare dual anesthetic remifentanil-propofol microemulsion ( Rem-Pro MM) and evaluate the anesthetic effects in adult dogs by continuous infusion. Methods:The Rem-Pro MM was prepared by the high pressure homogenization technolo-gy. The Rem-Pro MM was characterized with the particle size distribution, zeta potential and morphology as the indices. The anesthetic effects in adult dogs were evaluated by continuous infusion of Rem-Pro MM. Results:The particle size distribution, polydispersion in-dex and zeta potential of Rem-Pro MM was (217. 6 ± 48. 2) nm, (0. 212 ± 0. 032) and ( -31. 2 ± 3. 8) mV, respectively. The Rem-Pro MM was found to be small and homogeneous under a transmission electron microscope. The pharmacodynamic studies showed that the Rem-Pro MM in adult dogs with partial spleen surgery had promising anesthetic effects during the anesthesia induction and mainte-nance procedure. Conclusion: The dual anesthetic remifentanil-propofol microemulsion has good effects of sedation and analgesia, which is expected to be used in clinics.

Objective To explore the effect of case checking and head nurses′ comment on clinical nurisng rounds. Methods Three hundred hospitalized patients from January to December of 2012 were set as the control group , which received the traditional nursing ward round model. Another three hundred and thirty patients hospitalized from January to December of 2013 were set as the observation group, which received case tracking method integrated with head nurses ward round model. The ward nursing management quality of both groups including the ward management, grading nursing, basic nursing, nursing safety, health and education and the qualified rate of nursing documents were compared between the two groups. Result The quality of nursing management and nursing documents qualification rate of the observation group were obviously higher than those of the control group. Conclusion The case tracking method and the head nurses′comments can improve the quality of clinical nursing rounds , enhance nurses comprehensive quality and increase management level of the head nurses.

Bronchial asthma is one of the most common chronic respiratory diseases in children.Airway remodeling that makes asthma difficult to control is an important pathological progress of asthma.Toll-like receptors (TLR), a kind of pattern recognition receptors, are expressed in a variety of human airway structure cells, such as airway epithelial cells, smooth muscle cells, mucus gland cells, etc.It is reported that TLRs play important roles in the regulation of airway remodeling in asthma.This article briefly summarizes the role of TLR in airway remodeling of asthma.

In monozygotic monochorionic diamnionic twin pregnancy,twin-twin transfusion syndrome is one of the most serious complications and often exhibit twin growth not well proportioned, abnormal amniotic fluid volume and other complications.Twin anemia-polycythemia sequence is a special type of Twin-twin transfusion syndrome, and both have great difference in clinical manifestations, diagnosis and prognosis of neonatal stages.This article compares with the pathogenesis, diagnostic criteria, treatment methods and prognosis of these two diseases.