Currently targeted therapy of breast cancer therapy has become the hot research field and a kind of brand-new biological treatment mode after three traditional pattern of the surgery, radiotherapy and chemotherapy for breast cancer. Molecular targeted therapy is aimed at the target that may cause cancer cells, such as protocarcinogenic genes and tumor-suppressor genes, cell signaling pathways, cytokines and receptors, antiangiogenesis etc. It can reverse malignant biology behavior to inhibit tumor cell growth from the molecular level and has the advantages of high specific effects and low side effects. This paper focuses on the drug of the molecular targeted therapy for breast cancer and the latest progress of targeted therapy.

Objective To establish the quality standard for Kangyuan Granula. Methods Radix Astragali, Radix Paeoniae Alba, Fructus Lycii and Radix et Rhizoma Glycyrrhizae in this prescription were identified by TLC. The content of the Paeoniflorin was determined by HPLC. The column of AichromBond-AQ-C18 (4.6 mm?150 mm, 5 ?m) was used, the mobile phase was Water-Acetonitrile (85∶15), at the flow of 1.0 mL/min, the column temperature of 30 ℃, and peaks were detected at 230 nm. Results The characteristic of identification by TLC was distinct and highly specific. The linear range of Paeoniflorin was 0.0545～0.545 ?g, r =0.999 5, the average recovery was 97.90%, RSD=1.06% (n =6). Conclusion The method was easy to operate with accurate result and good reproducibility. It can be used effectively for the quality control of this preparation.

Ischemic stroke is one of the major diseases of causing adult disability and death.Age,sex,smoking,hypertension,and diabetes,etc.are the risk factors for ischemic stroke,but they can only partially explain the reasons of stroke onset.Twins,families,and single-gene genetic stroke studies have shown that ischemic stroke has obvious genetic predisposition.In recent years,genomics has made remarkable progress in ischemic stroke study and has shown that many new single nucleotide polymorphisms or genes are associated with ischemic stroke and its risk factors.However,the contribution of these genetic genes for ischemic stroke is still too small and the repetitive studies are needed for further confirmation.

OBJECTIVE: To establish a bacterial endotoxin test method for fluorescein sodium injection.METHODS: Based on the bacterial endotoxin test method stated in Chinese Pharmacopoeia 2005 edition(second part),an interference test was conduced on different batches of fluorescein sodium samples using tachypleus amebocyte lysate from different companies.RESULTS: At a concentration of no more than 0.75 mg?mL-1,fluorescein sodium showed no interfere with the bacterial endotoxin test.CONCLUSION: It is feasible for bacterial endotoxin test of fluorescein sodium injection be conducted in a limit value of 0.15 EU?mL-1.

Bronchial asthma is one of the most common chronic respiratory diseases in children.Airway remodeling that makes asthma difficult to control is an important pathological progress of asthma.Toll-like receptors (TLR), a kind of pattern recognition receptors, are expressed in a variety of human airway structure cells, such as airway epithelial cells, smooth muscle cells, mucus gland cells, etc.It is reported that TLRs play important roles in the regulation of airway remodeling in asthma.This article briefly summarizes the role of TLR in airway remodeling of asthma.

Objective To explore clinical efficacy of percutaneous tiansforaminal endoscopic discectomy on postoperative complications of patients with the protrusion of lumbar intervertebral disc.Methods Sixty cases of lumbar protrusion of the intervertebral disc were selected as our subjects from Nov.2012 to Nov.2013 who were hospitalized in the Seven People's Hospital of Zibo.Of which,30 cases were preformed lumbar protrusion of the intervertebral disc through transforaminal endoscopic treatment.Another 30 case were with small incision fenestration laminectomy discectomy.The information of hospitalized periods,bleeding volume,operation incision length and periods within the bed were collected.Visual analogue scale (VAS) was performed at postoperative 24,48 h.The Oswestry disability index (ODI) was applied to evaluated the daily life ability assessment of patients before surgery,1 month and 6 months after surgery.Results The incision length,intraoperative bleeding volume,postoperative bed and hospitalization periods in patients with transforaminal endoscopic treatment were ((0.7 ± 0.2) cm,(11.6 ± 2.2) ml,(27.5 ± 8.2) d,(3.3 ± 1.1) d respectively,less than that in patients with small incision fenestration laminectomy discectomy ((4.9 ± 0.2) cm,(102.9 ±16.3) ml,(94.1 ±19.7) d,(8.3 ±2.1) d).The differences were significant (t =81.332,30.403,17.095 and 11.552;P＜0.05).VAS in patients with transforaminal endoscopic treatment and in patients with small incision fenestration laminectomy discectomy were (3.7 ± 1.2) and (6.8 ± 1.9),(2.1 ± 1.1) and (5.3 ±1.6),(1.4 ±0.9) and (4.4 ± 1.5) respectively,and the differences were significant(t =7.555,9.026,9.393 ; P ＜ 0.05).The ODI in patients with transforaminal endoscopic treatment at 1 and 6 months after surgery were 12.1 ± 4.7 and 18.6 ± 3.6,higher than that in patients with small incision fenestration laminectomy discectomy(5.4 ± 2.3,13.2 ± 2.7),and the differences were significant (t =6.013,12.045 ; P ＜ 0.05).Conclusion Lumbar percutaneous transforaminal endoscopic discectomy is with obvious superiority than the method of small incision fenestration laminectomy discectomy in terms of the operation incision,intraoperative bleeding volume,postoperative bed and hospitalization time.Meanwhile,it shows the less damage to the spinal stability structure,and quick recovery.

The TCM spirit cultivation theory of the old is both extensive and profound. It can not only provide guidance to the establishment of the TCM spirit cultivation system of the old under the aging background, but also provides vast development prospects to develop the TCM spirit cultiva-tion education of the old in medical college. Some particular measures to be taken are as the followings:to establish relevant courses about the TCM spirit cultivation education of the old as a specialty course for clinical medicine about gerontology, nursing and rehabilitation therapeutics; students participate in social practice program in community, retirement organization and geriatric ward; to integrate teaching resources and set up the excellent teachers' team;to ensure teaching process designs scientific, includ-ing the improvement of teaching methods, diversified styles of assessment and online courses design.

The essence of the market-oriented reforms is to promote competition rather than privatization. Competition does not only exist between public and private ownership forms, but also among public hospital them-selves. The promotion of the internal competition among public hospitals will play a positive role for the market mechanism to take effect. Due to the unique in various aspects of health care market, competition can have positive or negative influences on performance, thus the policy makers need to guide the competition policies carefully to make sure they work in the right direction. In regard to the status quo, we provide the following policy suggestions which emphasize on stronger regulation, higher degree of autonomy for lower-level medical institutions, and more information disclosure.

Objective: Medical equipment acceptance check is very important for proper operation of new medical equipment. Methods: Acceptance of medical equipment is tender documents and contracts based on configuration parameters in the tenders and contracts, performance indicators, with the goods packing list, one by one detailed check. Results: In order to ensure equipment of early procurement is as same as the actual installation equipment. Conclusion: Medical equipment acceptance is the final step of the equipment entering the hospital, Which directly related to the quality of medical equipment and hospital benefit.

Objective To investigate the distribution of pathogenic microbes in blood culture and analysis of drug resis-tance of common pathogens in children with acute leukemia.Methods BACTE-FX system made by BD company was applied for the blood culture of children with acute leukemia. API or VITEK-2 Compact bacterial identiifcation system was used for iden-tiifcation of bacteria. Antimicrobial susceptibility testing was performed by disk diffusion method.Results Among 6400 blood culture samples in children with acute leukemia, 713 cases were positive at the rate of 11.1%. Among 655 strains ifrst isolated from the patient, Gram-positive bacteria were 52.5% (344/655) and dominated byStaphylococcus. Strains resistant to vancomy-cin and linezolid were not found. 278 strains (42.5%) were Gram-negative bacteria , andS.marcescens andE.coli were dominat-ed. Gram-negetive bacteria showed high antibacterial activity to imipenem with the sensitive rate of 100%. 33 strains (5%) were fungi.Conclusions Staphylococcus,S.marcescens, and E.coli were the most important bacteria in blood culture of children with acute leukemia. We can offer effective help for clinical treatment with the susceptibility testing.