OBJECTIVE: To establish a bacterial endotoxin test method for fluorescein sodium injection.METHODS: Based on the bacterial endotoxin test method stated in Chinese Pharmacopoeia 2005 edition(second part),an interference test was conduced on different batches of fluorescein sodium samples using tachypleus amebocyte lysate from different companies.RESULTS: At a concentration of no more than 0.75 mg?mL-1,fluorescein sodium showed no interfere with the bacterial endotoxin test.CONCLUSION: It is feasible for bacterial endotoxin test of fluorescein sodium injection be conducted in a limit value of 0.15 EU?mL-1.

Currently targeted therapy of breast cancer therapy has become the hot research field and a kind of brand-new biological treatment mode after three traditional pattern of the surgery, radiotherapy and chemotherapy for breast cancer. Molecular targeted therapy is aimed at the target that may cause cancer cells, such as protocarcinogenic genes and tumor-suppressor genes, cell signaling pathways, cytokines and receptors, antiangiogenesis etc. It can reverse malignant biology behavior to inhibit tumor cell growth from the molecular level and has the advantages of high specific effects and low side effects. This paper focuses on the drug of the molecular targeted therapy for breast cancer and the latest progress of targeted therapy.

Objective To establish the quality standard for Kangyuan Granula. Methods Radix Astragali, Radix Paeoniae Alba, Fructus Lycii and Radix et Rhizoma Glycyrrhizae in this prescription were identified by TLC. The content of the Paeoniflorin was determined by HPLC. The column of AichromBond-AQ-C18 (4.6 mm?150 mm, 5 ?m) was used, the mobile phase was Water-Acetonitrile (85∶15), at the flow of 1.0 mL/min, the column temperature of 30 ℃, and peaks were detected at 230 nm. Results The characteristic of identification by TLC was distinct and highly specific. The linear range of Paeoniflorin was 0.0545～0.545 ?g, r =0.999 5, the average recovery was 97.90%, RSD=1.06% (n =6). Conclusion The method was easy to operate with accurate result and good reproducibility. It can be used effectively for the quality control of this preparation.

Ischemic stroke is one of the major diseases of causing adult disability and death.Age,sex,smoking,hypertension,and diabetes,etc.are the risk factors for ischemic stroke,but they can only partially explain the reasons of stroke onset.Twins,families,and single-gene genetic stroke studies have shown that ischemic stroke has obvious genetic predisposition.In recent years,genomics has made remarkable progress in ischemic stroke study and has shown that many new single nucleotide polymorphisms or genes are associated with ischemic stroke and its risk factors.However,the contribution of these genetic genes for ischemic stroke is still too small and the repetitive studies are needed for further confirmation.

The incidence of bronchial asthma in children is increasing nowadays.More and more studies have showed that epigenetics plays an important role in the pathogenesis of asthma.One of them are histone modification(acetylation and deacetylation),which is catalyzed by histone acetyltransferase (HAT) and histone deacetylase (HDAC).HAT and HDAC can mediate asthma via causing Th1/Th2 imbalance,increased airway hyperresponsiveness,regulation of various inflammatory medium and so on.This paper reviews the HAT and HDAC mediated incidence of asthma and other possible mechanisms,the latest of glucocorticoid in the treatment of children with asthma epigenetic mechanisms.

Kawasaki disease,also known as mucocutaneous lymph node syndrome,is an acute,febrile,multisystem disease in children.The coronary artery lesions caused by Kawasaki disease has become the most common acquired heart disease in children.In recent years,epidemiological investigation on Kawasaki disease has been launched in the world.It can provide the basis for clinical diagnosis and treatment of Kawasaki disease through summarizing the epidemiological characteristics of Kawasaki disease in the different regions in China in recent 20 years,including the characteristics of incidence,age,gender,nationality,family history,seasonal onset,relapse rate,mortality and heart damage.

[Objective] To study the combination of traditional Chinese and western medicine treatment of tibial plateau fractures cases for clinical observation.[Methods] Randomly select 72 cases of patients in our hospital in 2010-2011, April, make a diagnosis and give treatment with fracture of tibial plateau, divide into two groups, control group 36 cases were treated by routine operation fixed and functional recovery exercise therapy; The team of 36 patients based on treatment with TCM. [Results] The research group patients, excellence rate was significantly higher than the control group, there was significantly statistical differences. Two groups of patients in adverse reactions, through statistical analysis of clinic, research group was better than control group, with statistical difference.[Conclusion] Combining traditional Chinese and western medicine treatment of tibial plateau fracture, the clinical curative effect is distinct, can accurately determine fracture position, select the appropriate clinical treatment, help patients recover at an early date, worthy of clinical application for a long time.

Locally advanced non-small cell lung cancer (LA-NSCLC) usually is inoperable because of tumor progress.At present,concurrent chemoradiotherapy has become standard regimen of these patients,which has gotten remarkable effectiveness.This article is a summarization on concurrent chemoradiotherapy for LA-NSCLC.

ObjectiveTo explore the influence of different hemostatic measures of laparoscopic ovarian cyst removed on ovarian function.MethodsSeventy patients with ovarian cyst who performed laparoscopic ovarian cysts removed between January 2009 and December 2010 were randomly divided into group Ⅰ (23 cases) and group Ⅱ (47 cases) by mechanical sampling.Group Ⅰ with bleeding electrocautery,group Ⅱ with suture.Estradiol,follicle puncture hormone (FSH),luteinizing hormone (LH),number of sinusoidal follicles,normal ovarian function and the number of declining ovarian reserve were compared in two groups before operation and after 6 months operation.ResultsAfter operation,the proportion of group Ⅱ with normal ovarian function was significantly higher than that of group Ⅰ[80.9％(38/47) vs.39.1％(9/23),P ＜ 0.05 ],estradiol and number of sinusoidal follicles were significantly higher than those of group Ⅰ [( 198.4 ± 24.4) pmol/L vs.( 153.4 ± 16.8) pmol/L,7.1 ± 2.4 vs.5.8 ± 0.9](P＜ 0.05),FSH and FSH/LH were significantly lower than those of group Ⅰ[ ( 10.58 ± 4.57) U/L vs. ( 13.72 ± 2.58) U/L,2.34 ± 1.32 vs.2.82 ± 0.88 ] (P ＜ 0.05).ConclusionDuring laparoscopic ovarian cyst removed,using electrocautery can significantly reduce residual ovarian function,but using suture hemostasis can effectively protect residualovarian function.

Objective To evaluate the clinical efficacy of procedure for prolapse and hemorrhoids (PPH)plus Milligan-Morgan methods for circumferential mixed hemorrhoid.Methods A total of 74 cases with circumferential mixed hemorrhoid were treated by PPH plus Milligan-Morgan methods.Results The anal form of all cases were changed correctly.There was no complication such as anal edema,incontinence,anal stenosis,postoperative massive bleeding and infection.Conclusion PPH plus Milligan-Morgan methods is a safe and effective way for circumferential mixed hemorrhoid.This way can decrease postoperative complications and improve the curative effect.