In this experimental study, Wistar rats were divided into 3 groups: Group A, 33 rats fed with diet purchased from endemic goitre area and iodine-free distilled water; Group B, 33 rats fed with the same diet of group A but 50% of casein was added and iodine-free distilled water; Group G, 33 rats fed with general rat's diet and ordinary tap water as control. After three and a half months, blood taken from ocular vein was measured for thyroid hormone and thyroid-stimulating hormone, and thyroid glands were taken for histologic examination.The results have proved that typical iodine deficient goitre and hypothyroid models have been successfully established in rats from group A and casein can prevent rats from iodine-deficient goitre and hypothyroidism. The mechanism might be that casein may enhance iodine transportation and utilization in thyroid.

AIM: To investigate the effect of Astragalus membranaceus on the balance of oxidation and antioxidation in bleomycin-induced pulmonary fibrosis in mice and the possible anti-fibrosis mechanism of Astragalus membranaceus.METHODS: Female KM mice (n=36) were randomly divided into 3 groups.The mice in control group were administered with saline aerosol intratracheally.The mice in fibrosis group were administered with bleomycin at dose of 3 mg/kg aerosol intratracheally.The mice in Astragalus membranaceus group were administered with bleomycin at dose of 3 mg/kg aerosol intratracheally and then intraperitoneal injected with Astragalus membranaceus parenteral solution at daily dose of 1.7 g/kg.All mice were sacrificed 14 d after the treatment, and the lungs and serum were collected for detection.Hematoxylin-eosin staining was performed in the lung tissue.The mRNA expression of superoxide dismutase 1/2/3 (SOD1/2/3), catalase (CAT), NADPH oxidase 2/4 (NOX2/4) and α-smooth muscle actin (α-SMA) was detected by RT-PCR, and the protein expression of α-SMA and NOX2/4 was determined by Western blot.The concentration of malondialdehyde (MDA) and total antioxidant capacity (T-AOC) in the serum were measured by a colorimetric method.RESULTS: The pathological injury was obviously observed in bleomycin group compared with control group, but was attenuated in Astragalus membranaceus group.The α-SMA mRNA and protein expression, MDA/T-AOC, NOX2, NOX4 and SOD3 mRNA expression, and NOX2 protein expression in bleomycin group were significantly higher than those in control group, while those in Astragalus membranaceus group were significantly lower than those in bleomycin group.The protein expression of NOX4 in bleomycin group was significantly lower than that in control group, while that in Astragalus membranaceus group was higher than that in bleomycin group.The mRNA expression of SOD1 and CAT in Astragalus membranaceus group and bleomycin group were decreased compared with control group.No significant difference of SOD2 mRNA expression among the 3 groups was observed.CONCLUSION: Astragalus membranaceus inhibits bleomycin-induced pulmonary fibrosis in mice by maintaining the balance of oxidation and antioxidation.

The ability to construct knowledge from data is displayed more significantly in data literacy than that of traditional information literacy.The connotation of medical data education was summarized,the current situation of domestic and foreign medical data literacy education was reviewed,and the necessity of data literacy education in medical colleges and universities was analyzed,with suggestions put forward for the target of data literacy teaching,allocation of teaching resources,and design of teaching.

Fifty cases of HCC with 105 procedures of TAI/TAE performed by DSA system were analysed retrospectively.Improperly operative technique used in the procedure of TAI/TAE was found in sixteen cases of this series,included:unsuccessfully superselec- tive catheterization of the hepatic artery in 6 cases,resulting from the unskillful catheteriza- tion or the abnormality of target arteries,etc.Failure recatheterizations of the hepatic arter- ies in the initial or subsequent procedures of TAI/TAE were occurred in the other 6 cases. (because of the stenosis/obstruction of hepatic artery resulted from the intimal injury of the target artery by repeated catheterization,the infusion of anticancer drugs(MMC,5-Fu)and excessive embolization of hepatic artery with gelatine sponge,ete);In the remaining 4 cas- es,the tumor's parasitic vessels-inferior phrenic arterier were neglected due to no abdominal aortography was performed.The results of 16 cases with improperly operative technique of TAI/TAE showed that the decrease of tumor's size was frequently less than 50 per cent,or no change,and in few cases the size of tumor became larger than before.Comparing the de- crease rate of tumor in this 16 cases with the comparative group also confirmed the presence of the significant difference(P

Object To study the chemical constituents in Ligustrum sinense Lour.Methods One new and four known phenolic glycosides were isolated and identified by column chromatography, MS, IR and NMR data.Results Five phenolic and two lignan components were isolated from water soluble part in methanol extract of the stem and leaves of L.sinense.The obtained compounds were identified as sinenosides I, 8′-?-hydroxyl-lariciresinol-4′-O-?-L-glucopyranoside (Ⅰ), a new compound; liriodendrin (Ⅱ); kaempferol-3-O-?-D-glucopyranoside (Ⅲ); 7-O-?-L-rhamnopyransyl-kaempeferol-3-O-?-D-glucopyranoside (Ⅳ), 7-O-?-D-rhamnopyransyl-kaempeferol-3-O-?-D-rhamnopyranoside (Ⅴ).Conclusion All the compounds were isolated from this plant for the first time.

Objective:To assess the feasibility of dilute sulfuric acid injected into the rabbit liver in vivo,and to provide experimental proof for the interventional therapy on hepatocellular carcinoma.Methods:10 big white rabbits were randomly divided into two groups.10% sulfuric acid was injected into liver at a dose of 1ml.In the first group,the biochemical changes in serum were examined before and immediately,3,7,and 15 days after the procedure.In the second group,histopathological findings of the liver were observed after 10% sulfuric acid injected into liver immediately,3,7,15 and 30days postoperatively.Results:The ALT and AST level in serum had transient elevation,but shortly returned to the same level as before the procedure.There was no significant elevation of ALB,ALP,?-GGT,TBIL,DBIL and BUN level after the procedure.Pathological changes indicated hepatocellular degeneration,coagulative necrosis,inflammatory cells infiltration and granulation tissue proliferation.Conclusion:Diluted sulfuric acid have strong power of cells inactivation,but without obvious toxicity and side-effect,which could be a new type of tumor cells inactivation material for the treatment of hepatocellular carcinoma local ablation.

As an important branch of interventional radiology and pediatric radiology, pediatric interventional radiology has been widely accepted by both clinicians and patients' parents due to its marked efficacy and less invasiveness, but its prevalence is still yet to be recommended. This paper overviews the current status of pedatric interventional radiology in our country, its specificity during the clinical management, and existing problems to be solved.

Objective:To Amplify,purify and identify the cDNA library of human K562 cell in E.coli DH5? and to transform it into yeast cell for subsequent target protein screening.Methods:The cDNA library of human K562 cell was amplified.Then the library plasmids were extracted and transformed into Y187 yeast cell.The results of transformation were verified by PCR and restriction enzyme analysis.Results:The cDNA library of human K562 cell was amplified successfully and the diversities were verified.The cDNA library of human K562 cell was also successfully transformed into Y187 yeast cell.Conclusion:Successfully amplification,purification and identification of the cDNA library of human K562 cell lay the foundation for the subsequent protein screening.

Transcatheter hepatic arterial chemoembolization (THACE)has been widely used in the treatment of advanced primary hepatic carcinoma (PHC)and metastatic hepatic carcinoma (MHC). Although the incidence of severe complications associated with THACE is unusual (0% ～ 5% ),its prognosis is generally worse and mortality is higher than that of postembolization syndrome.Therefore,to minimize the risk associated with THACE has to be achieved through the understanding of these severe complications related to the selection of indication,the use of chemoembolic agents and manipulation of a cathether or guide wire,etc. In this paper,according to the involved anatomic organs,these severe complications are divided into six categories as follows:(1)complications of the liver,including acute hepatic failure,liver infarction (necrosis),liver abcess and liver (or tumor)ruptrure;(2)complications of the celiac artery and its branches,included of iatrogenic dissection,stricture or occlusion,perforation or pseudoaneurysm and multiple intrahepatic aneurysms; (3)complications of intrahepatic biliary system,included of cholecystitis and gallbladder infarction,bile duct necrosis and intrahepatic biloma formation; (4)complications of extrahepatic structures,included of nontarget embolism or infarction of the brain,spinal cord,lung,spleen,gastroduodenum and pancreas; (5)bleeding of upper digestive tract; (6)the orthers. And the incidence,pathogenesis,predisposing factors,clinical and imaging manifestations of these complications are also discussed in detail,it may be of great advantage to its correct diagnosis promptly and appropriate management.

There were increasing studies on the association of periodontal diseases with coronary heat disease (CHD) in the recent 20 years. This article reviewed the evidence supporting the association between periodontal disease and CHD, the possible mechanisms explaining the association, and the possible effect of periodontal treatment on the risk of CHD. In general, it is suggested that periodontal disease especially periodontitis is modestly associated with CHD. Besides there are some common risk factors, such as smoking, stress, elder age, male gender and low socioeconomic status, between these two diseases, long chronic periodontal infections by periodontal pathogens, the systemic acute-phase response and host immuno-inflammatory response to the exposures of periodontal infection appear to be the important mechanisms for connecting the periodontal disease and CHD. Periodontal treatment can decrease the infection of periodontal pathogens and therefore, reduce the systemic inflammatory burden. It was observed in some studies that periodontal treatment could reduce the serum inflammatory biomarkers such as C-reactive protein and improve endothelial function. Even though the studies on effect of periodontal interfere on CHD are still limited now, it appears hopeful that periodontal treatment could reduce the risk of CHD and therefore become one of the preventing strategies for CHD.