ObjectiveTo explore the diagnosis value of color Doppler ultrasound of the carotid atherosclerosis plaques in diabetic patients. MethodsColor Doppler ultrasonic assessment of carotid was performed in 124 diabetic patients(test group)and 100 normal controls(control group).The intima-media thickness(IMT)and the carotid atherosclerosis plaques parameters were measured.The results were compared and analyzed between two groups. ResultsThe IMT and the incidence rate of carotid atherosclerosis plaques in the test group were higher than those in the control group(all P＜0.05).There was no statistical difference in the site of the plaques between test and control group(P＞0.05). ConclusionThe incidence rate of the carotid atherosclerosis of type 2 diabetes was higher.Carotid color Doppler ultrasonography was an optimal technique for screening and diagnosing atherosclerosis.

Objective To explore the clinical efficacy of percutaneous vertebroplasty technique(PVP) in the patients with senile osteoporotic thoracolumbar compression fractures (OVCFs) and to explore the main factors of PVP.Methods PVP treatment of OVCFs 46 cases,in the perspective of C-arm X-ray,bone cement as a filling material,transpedicular puncture and injection of 59 vertebrae.Results The pain in 35 cases(76.09％) OVCFs patients apparent ease or disappear,the remaining 11 cases of moderate relief;follow-up period(3 to 15)months(mean 8.6months).After X-ray review,the patient' s bone cement dispersion was good and has improved vertebral height has not been lost,activity and quality of life significantly inproved.Conclusion PVP is simple,less invasive,etc.,which can quickly reduce pain and improve their quality of life.

Liver transplantation rapidly has become the best choice for pediatric patients with end-stage and metabolic liver disease, since the first liver transplantation succeeded in 1989. In pediatric liver transplantation, significantly improved short-term and long-term survival is attributed to advances in surgical techniques and reasonable immunosuppression regimens in the last 20 years. However, postoperative infections, which remain the most common complication following pediatric liver transplantation, severely affect posttransplantation morbidity and mortality. This paper summarizes the infections after liver transplantation in children and rational drug use.

ObjectiveTo investigate the clinical efficacy of primary and connecting point combination acupuncture in treating mild and moderate depression.MethodPatients meeting the inclusion criteria were randomly allocated to observation and control groups. Both groups of enrolled patients took fluoxetine hydrochloride (Prozac). The observation group received meridian-unblocking acupuncture at yuan-primary points and luo-connecting points and the control group, acupuncture at routinely selected points in the eleventh-five textbook. Treatment was given for four consecutive weeks. The Hamilton Depression Scale (HAMD) 24-item score was recorded in the patients before and after one month of treatment. The therapeutic effects were evaluated according to the score reduction rate in the two groups.ResultThere wasa statistically significant post-treatment difference in the HAMD 24-item score between the two groups (P<0.05). The total efficacy rate was83.3% in the treatment group and70.0% in the control group. There was a statistically significant post-treatment difference in therapeutic effect between the two groups (P<0.05). ConclusionMeridian-unblocking acupuncture at yuan-primary points and luo-connecting points is more effective than conventional acupuncture in proving the symptoms in patients with mild or moderate depression.

The American College of Gastroenterology( ACG)released the first clinical guideline for drug-induced liver injury( DILI)in June,2014:the diagnosis and management of idiosyncratic drug-induced liver injury( IDILI), which includes all aspects of IDILI,such as risk factors,diagnosis,differential diagnosis and treatment. The guideline discusses especially the issues on herbal and dietary supplements( HDS)induced liver injury and DILI in patients with chronic liver disease. The guideline is of great value for standardizing the clinical diagnosis and treatment,providing the best recommendations for management of the patients with DILI. Since the evidence of research in the field of DILI is relatively weak at this moment,the clinicians should consider the possible individual difference of the patients under the frame of guideline for making the best clinical decision.

Objective To investigate the clinical efficacy of trimetazidine combined with atorvastatin in the treatment of patients with coronary heart disease and its influence on serum lipid and hemorheology.Methods 140 cases with coronary heart disease were randomly divided into two groups.The control group was given trimetazidine on the basis of the conventional treatment,while the observation group was given atorvastatin on the basis of the control group.The clinical efficacy,changes of hemorheology and blood lipid were compared between the two groups.Results The effective rate of the observation group was 95.71％,which was significantly higher than 77.14％ of the control group (x2 =10.620,P ＜ 0.05).After treatment,TC,TG,LDL-C and HDL-C of the observation group were (2.54 ± 0.32) mmol/L,(1.41 ± 0.32) mmol/L,(1.53 ± 0.33) mmol/L and (0.73 ± 0.35) mmol/L,which were significantly lower than those of the control group (t =7.42,8.09,8.11,9.03,all P ＜ 0.05).After treatment,the nbh,nbl,np and HCT of the observation group were (5.11 ± 0.51) mPa/s,(8.03 ± 1.12) mPa/s,(1.02 ± 0.21) mPa/s and (40.34 ± 3.28) ％,which were significantly lower than those of the control group(t =8.33,9.14,8.08,9.44,all P ＜ 0.05).Conclusion Trimetazidine combined with atorvastatin in the treatment of coronary heart disease can significantly improve patients' lipid and hemorheology,and it can be promoted and applied in clinical practice.

AIM: To establish the quality standard of Wumeirendan (Fructus Mume, Radix et Rhizoma Glycyrrhizae, Mentholm, etc.) METHODS: Fructus Mume was identified by TLC. Menthol was determined by GC, Ammonium glycyrrhiziate was determined by HPLC. RESULTS: The average recoveries were 103.5% and 100.3%, RSD were 0.76% and 1.03%. The TLC sports developed were fairly clear, and the blank test showed no interference. CONCLUSION:The method developed is simple and accurate with good reproducibility, and the method can be used for quality control of Wumeirendan.

The isolation purification, iodination and identification of a-Bungarotoxin(a-BTX)were recorded.Purified a-BTX was prepared from the venom of Hunan Bu-ngarus multicinctus (Blyth) by CM-Sephadex C~50 ionexchange chromatography and Sephadex G~25 chromatography.The homogeneous a-BTX was obtained by rechromatography of purified ct-BTX (fraction 3) on a CM-Cellulose CM-32, and was labelled with 1251 by chloramine-T method.The 1251-?-BTX was then separated from Na125I and purified by Sephadex G-25 column.The results yield a specific activity of 90~100 Ci/mM.40~50% labelling.40~60% recovery of protein and less than 3 % contamination with free iodine in 125I-?-BTX.The purified a-BTX was identified by determining its N-terminal amino acid, amino acid composition, isoelectric and polyacrylamide gel electrophoresis.Biological activity of the ?-BTX was determined by its toxicity to mice, its neuromuscular blocking action and binding to acetylcholine receptors at the motor entplate of the rat diaphragm.

There are more complicated pathological changes in the developing brain with invasive infection of central nervous system(CNS) which absolutely play predominant role in neurodevelopmental disability and mortality in spite of a lower prevalence during neonatal period.Bacterial meningitis demonstrated by cerebrospinal fluid(CSF) culture only remain difficult since poor positive findings,in the severe condition more complications could be often found such as vasculitis,ventriculitis,cerebritis and abscess.Cerebral abscess could be found independently without abnormal CSF examination;while the cerebritis is one of the primary pathological changes in viral invasive infection of CNS,Enterovirus/human parechovirus often involved not only grey matter,but also the periventricular and subcortical white matter that sound similar to the findings of periventricular leukomalacia on magnetic resonance imaging(MRI).Finding the complications of CNS infections early and accurately describe pathological changes by reasonable using MRI can enhance the therapy and predictive ability.

OBJECTIVE:To establish a method for the simultaneous determination of geniposide,paeoniflorin,berberine hy-drochloride,baicalin and paeonol in Qingwei huanglian pill. METHODS:HPLC was performed on the column of Diamonsil C18 with mobile phase of acetonitrile-0.3% phosphate(gradient elution)at flow rate of 1.0 ml/min,detection wavelength was 238 nm, column temperature was 30 ℃ and the injection volume was 10 μl. RESULTS:The linear range was 15.36-153.6 μg/ml for genipo-side, 6.56-65.6 μg/ml for paeoniflorin, 22.74-227.4 μg/ml for berberine hydrochloride, 26.06-260.6 μg/ml for baicalin and 5.57-55.7 μg/ml for paeonol(r≥0.999 8);RSDs of precision,stability and reproducibility tests were not higher than 1.0%;recov-eries were 96.28%-99.38%(RSD=1.1%,n=6),97.13%-99.48%(RSD=1.2%,n=6),98.14%-100.25(RSD=0.7%,n=6), 97.38%-100.05%(RSD=1.0%,n=6) and 96.30%-99.12%(RSD=1.2%,n=6),respectively. CONCLUSIONS:The method is simple and good reproducibility,and suitable for the contents determination of geniposide,paeoniflorin,berberine hydrochloride, baicalin and paeonol in Qingwei huanglian pill.