Objective　To research the anterior corneal stromal puncture therapeutic effect for bullous keratopathy.Methods　The causes of the bullous keratopathy of the 11 cases were analysed and the bullous keratopathy was cured by the anterior stromal puncture methods.Results　Eleven cases were followed up for three months.The symptoms were relieved in all cases. The corneal bullous disappeared.Conclusion　The cases of the bullous keratopathy caused by mechanical or physical factors can be cured more effectively by using anterior corneal stromal puncture therapeutics.

NO is an important signal factor existing in expiratory air of normal person.Different kinds of airway inflammation can cause the increase of NO,especially eosinophilic inflammation.Fractional exhaled nitric oxide(FENO) can result in airway inflammation,which is noninvasive,repeatable and easily performed.It can be very helpful in the diagnosis and differential diagnosis of airway symptoms such as recurrent wheezing and chronic cough.It can also help determine the state of the disease and prognosis.It is a very useful tool in the assessment and management of airway diseases,especially asthma.

Zinc supplementation can reduce the mean duration,the recurrence rate and mortality of diarrhea.Nonetheless,it has no effect on stool frequency or stool output.Zinc supplementation results in different effort for the difference of the duration of diarrhea,pathogens,zinc type and doses of zinc,and shows no effect in infants younger than 6 months.Using iron as a co-intervention can reduce the effect of zinc,but vitamin A or ORS have a synergistic effect with the meeting.Average baseline zinc levels did not contribute to variations in the effect size.Zinc supplements are unlikely to improve compliance with the treatment for diarrheal disease.Longer course,zinc alone,malnutrition,diarrhea,breast-feeding children are prone to vomiting after oral administration of zinc.

In order to make the comprehensive evaluation work of intellectual property of TCM industry more scientific and reasonable in our country, promote the protection of intellectual property of TCM industry, based on the survey of the domestic and foreign literature about evaluation of intellectual property and TCM industry, this article built the evaluation index system of intellectual property of TCM industry from five aspects:the number of intellectual property, the quality of intellectual property, the development of intellectual property, the implementation of intellectual property, and the management of intellectual property, and elaborated each index in detail.

AIM: To analyse the main chemical constituents of Shuanghuanglian Injection Power(Flos lonicerae japanicae,Radix scutellariae and Fructus forsythiae) by high performance liquid chromatography-electrospray ionization-mass/mass spectrometry. METHODS: The sample solution was analysed by a Zorbax C_18 column with a gradient mobile phase comprised of acetonitrile and water.Electrospray ionization mass spectrometry detector was used,full-scan detection mode was evaluated for the identification of the liquid chromatography peaks. RESULTS: To analyse the mass spectrum of every liquid chromatography peaks,we identified 18 molecular mass from the ion chromatogram of Shuanghuanglian Injection Powder,9 compounds were identified,including quinic acid,caffeic acid,three monocaffeoylquinic acids,dicaffeoylquinic acid,forsythin,forsythoside and baicalin. CONCLUSION: Quinic acid is identified from Shuanghuanglian Injection Power for the first time.High performance liquid chromatography-mass/mass spectrometry is emerging as an important tool in traditional Chinese medicine.

Objective To evaluate the effect of open reduction and internal fixation in the treatment of 50 patients with tibial plateau fractures(FTP),and its influence on knee functional recovery.Methods 50 patients with FTP were treated with open reduction and internal fixation.The operation time,blood loss,gypsum braking time,hospitalization time and fracture healing time were recorded,and the incidence of complication was analyzed as well.3 days after operation,imaging evaluation was performed to evaluate the effect of operation reduction.The patients were followed up for 6 months.The HSS scores before and after operation were compared,and the clinical efficacy was evaluated by HSS score.Results 50 FTP patients treatment with internal fixation were successful.The operation time was (101.36±15.63)min,the blood loss was (291.45±34.98)mL,the hospitalization time was (6.85±1.21)d,the postoperative plaster brake time was (20.35±3.67)d,the fracture healing time was (87.63±2.36)d.50 patients were all satisfied to reset,excellent reduction in 40 cases,good reduction in 7 cases,satisfaction in 3 cases,postoperative incision infection in 2 cases,pulmonary embolism in 1 case,nonunion in 1 case and fascia compartment syndrome in 1 case,the total incidence rate of complications was 10.0%.6 months after operation,the HSS scores(pain,function,range of motion,muscle strength,flexion deformity,joint stability) were significantly higher than before operation(t=12.070,17.241,19.986,10.850,21.098,10.238,all P=0.000).Of 50 cases with FTP,excellent in 23 cases,good in 19 cases,fair in 7 cases and poor in 1 case,the excellent and good rate was 84.0%.Conclusion Open reduction and internal fixation in the treatment of FTP gets satisfactory reduction effect,it has certain safety,and it can effectively promote fracture healing and knee functional recovery and improvement,its clinical effect is remarkable.

Increased understanding of the role of incretin hormones in maintaining glucose homeostasis has enabled the development of pharmacotherapies that target deficient incretin activity in type 2 diabetes mellitus.lncretin-based therapies could be classified into 2 categories with different mechanisms of action:glucagon-like peptide 1 ( GLP-1 ) receptor agonists ( e.g.liraglutide,exenatide twice daily,and exenatide once weekly),and the dipeptidyl peptidase 4 ( DPP-4 ) inhibitors ( e.g.sitagliptin,linagliptin,saxagliptin,and vildagliptin),which inhibit the breakdown of endogenous GLP-1.Both categories share some specific characteristics,such as glucose-dependent insulin stimulation and low incidence of hypoglycemia.But there are also different profiles in efficacy between these 2 classes.This article reviews the pharmacokinetics and clinical aspects of efficacy and safety of these 2 classes of incretin-based therapies and elucidates their roles in treating type 2 diabetes mellitus.

Objective To investigate the feasibility of the gene chip technique and multiplex PCR technique for detecting and screening foodborne pathogens .Methods The primer sequences were designed to target cells ,the biotin was adopted to label the re‐verse primer 5′end and the amino group was adopted to label oligonucleotide probe 5′end .The probe spotted on a solid support for preparing microarray ,PCR product was hybridized with microarray probe region ,PCR and hybridization reaction system was opti‐mized .Results The microarray technique could simultaneously detect multiple pathogens of Shigella ,Salmonella ,Klebsiella pneu‐moniae ,Brucella ,Proteus mirabilis ,Staphylococcus aureus ,Campylobacter jejuni ,etc .,which was easy to operate and had strong specificity .The sensitivity of bacterial pure cultures was 5 .0 × 102 CFU/mL ,the sensitivity of DNA detection was 0 .1 pg ,the coin‐cidence rate for detecting isolated bacteria was 100 % .The PCR detection system was established and optimized by using primers , the concentration of Mg2+ and the annealing temperature Tm value of 1 .5 mmol/L and 56 ℃ were determined ,the detection sensi‐tivity reached to 10 pg ,all the specific primers amplified bands could be amplified under this sensitivity .Conclusion The gene chip technique and multiplex PCR technique can effectively detect foodborne pathogens ,which provide a new idea for detecting pathogens with the high‐throughput screening and are worth popularization and application in the field of food safety .

Seroprevalence of Bluetongue virus (BTV) in goats from Hubei was investigated by a commercial competitive enzyme-linked immunosorbent assay kits. Blood samples (n= 1157) were collected during the year 2014 and 2015. The results showed that 13.31% (CI 95% 11.4%–15.4%) serum samples were positive for BTV antibodies in goats in Hubei. The prevalence of BTV antibodies in each region ranged from 1.32% to 27.70%, and differences among the regions were statistically significant (p < 0.01). The prevalence of BTV in male and female goats was 14.23% (95% CI: 11.3, 17.6) and 12.58% (95% CI: 10.1, 15.4), respectively, no significant difference in genders (p > 0.05). In different seasons, the seroprevalence were 8.94% (95% CI: 5.6, 13.3) in spring; 18.31% (95% CI: 14.5, 22.7) in summer; 23.08% (95% CI: 17.0, 30.2) in autumn and 6.98% (95% CI: 4.6, 10.0) in winter, respectively with a significant difference of the prevalence in the different seasons (p < 0.01).